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1.发明授权Non-human carbonyl hydrolase mutants, vectors encoding same and hosts transformed with said vectors 失效非人羰基水解酶突变体，编码相同的载体和用所述载体转化的宿主
公开(公告)号：US5182204A
公开(公告)日：1993-01-26
申请号：US807786
申请日：1991-12-11
CPC分类号： C12R1/07 , C11D3/386 , C12N15/102 , C12N15/75 , C12N9/54 , Y10S435/832
摘要： Novel carbonyl hydrolase mutants derived from the DNA sequences of naturally-occurring or recombinant non-human carbonyl hydrolases. The mutant carbonyl hydrolases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant carbonyl hydrolase to encode the substitution of an amino acid in the amino acid sequence of a precursor carbonyl hydrolase. Such mutants have properties which are different than the precursor hydrolase.
摘要翻译： 衍生自天然或重组非人羰基水解酶的DNA序列的新型羰基水解酶突变体。 突变体羰基水解酶通常通过体外修饰编码天然存在或重组的羰基水解酶的前体DNA序列获得，以编码前体羰基水解酶的氨基酸序列中的氨基酸的取代。 这些突变体具有与前体水解酶不同的性质。
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公开(公告)号：US5441882A
公开(公告)日：1995-08-15
申请号：US521010
申请日：1990-05-09
申请人： David A. Estell , James A. Wells , Richard R. Bott
发明人： David A. Estell , James A. Wells , Richard R. Bott
CPC分类号： C12R1/07 , C11D3/386 , C12N15/102 , C12N15/75 , C12N9/54
摘要： There are described methods for making mutant subtilisins, the methods comprising obtaining a DNA fragment from a Bacillus subtilisin and introducing a mutation into the fragment by substituting at least one amino acid, transforming a suitable host cell with the mutated DNA, recovering a mutant subtilisin and screening the mutant subtilisin for certain altered enzymatic properties.
摘要翻译： 描述了制备突变型枯草杆菌蛋白酶的方法，所述方法包括从枯草杆菌蛋白酶获得DNA片段，并通过用至少一个氨基酸取代突变进入该片段，用突变的DNA转化合适的宿主细胞，回收突变型枯草杆菌蛋白酶和 筛选突变的枯草杆菌蛋白酶以获得某些改变的酶特性。
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公开(公告)号：US5310675A
公开(公告)日：1994-05-10
申请号：US805605
申请日：1991-12-10
CPC分类号： C12R1/07 , C11D3/386 , C12N15/102 , C12N15/75 , C12N9/54
摘要： Recombinant host bacteria and plasmids for making the bacteria using recombinant DNA techniques are described. The plasmids contain DNA coding for subtilisin with an amino acid substitution. Expression of the plasmid DNA results in production of a modified subtilisin.
摘要翻译： 描述了使用重组DNA技术制备细菌的重组宿主细菌和质粒。 质粒含有编码具有氨基酸取代的枯草杆菌蛋白酶的DNA。 质粒DNA的表达导致修饰的枯草杆菌蛋白酶的产生。
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公开(公告)号：US5346823A
公开(公告)日：1994-09-13
申请号：US36592
申请日：1993-03-24
申请人： David A. Estell , James A. Wells , Richard R. Bott
发明人： David A. Estell , James A. Wells , Richard R. Bott
CPC分类号： C12R1/07 , C11D3/386 , C12N15/102 , C12N15/75 , C12N9/54
摘要： There are provided methods for making a mutant Bacillus subtilisin having altered oxidative stability, the methods comprising obtaining DNA fragment consisting of a region coding for a Bacillus subtilisin, and introducing a mutation into said DNA fragment such that the mutation is introduced in a region encoding a methionine, tryptophan, cysteine or lysine, sensitive to oxidation, such that upon expression of the mutant subtilisin one or more codon regions encoding for methionine, tryptophan, cysteine or lysine is replaced with an amino acid other than methionine, tryptophan, cysteine or lysine, preferably alanine or serine.
摘要翻译： 提供了制备具有改变的氧化稳定性的突变型枯草杆菌蛋白酶的方法，所述方法包括获得由编码枯草杆菌蛋白酶的区域组成的DNA片段，并将突变引入所述DNA片段，使得突变引入到编码 敏感的蛋氨酸，色氨酸，半胱氨酸或赖氨酸，使得突变型枯草杆菌蛋白酶表达后，编码甲硫氨酸，色氨酸，半胱氨酸或赖氨酸的一个或多个密码子区域被甲硫氨酸，色氨酸，半胱氨酸或赖氨酸以外的氨基酸替代， 优选丙氨酸或丝氨酸。
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公开(公告)号：USRE34606E
公开(公告)日：1994-05-10
申请号：US556918
申请日：1990-07-20
申请人： David A. Estell , James A. Wells , Richard R. Bott
发明人： David A. Estell , James A. Wells , Richard R. Bott
CPC分类号： C12R1/07 , C11D3/386 , C12N15/102 , C12N15/75 , C12N9/54
摘要： A cloned subtilsin gene has been modified at specific sites to cause amino acid substitutions at certain spots in the enzyme. The modified enzyme, preferably produced by Bacillus, is useful in combination with detergents.
摘要翻译： 已经在特定位点修饰了克隆的subtilisin基因，以在酶中的某些点处引起氨基酸取代。 优选由芽孢杆菌产生的修饰酶可与洗涤剂组合使用。
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公开(公告)号：US4760025A
公开(公告)日：1988-07-26
申请号：US614612
申请日：1984-05-29
申请人： David A. Estell , James A. Wells
发明人： David A. Estell , James A. Wells
CPC分类号： C12R1/07 , C11D3/386 , C12N15/102 , C12N15/75 , C12N9/54
摘要： A cloned subtilisin gene has been modified at specific sites to cause amino acid substitutions at certain spots in the enzyme. The modified enzyme, preferably produced by Bacillus, is useful in combination with detergents.
摘要翻译： 克隆的枯草杆菌蛋白酶基因已在特定位点被修饰，以在酶中的某些位点引起氨基酸取代。 优选由芽孢杆菌产生的修饰酶可与洗涤剂组合使用。
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公开(公告)号：US08685893B2
公开(公告)日：2014-04-01
申请号：US12181222
申请日：2008-07-28
IPC分类号： C40B40/02
CPC分类号： C40B40/02 , C07K14/005 , C07K2319/00 , C12N15/1037 , C12N2795/10022 , C12N2795/10043
摘要： The transformation yield of electroporation is increased by using higher DNA concentrations and DNA affinity purification. Fusion proteins of a viral coat protein variant and a heterologous polypeptide are useful in phage display systems.
摘要翻译： 通过使用更高的DNA浓度和DNA亲和纯化，提高了电穿孔的转化产率。 病毒外壳蛋白变体和异源多肽的融合蛋白可用于噬菌体展示系统。
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8.发明授权公开(公告)号：US08679771B2
公开(公告)日：2014-03-25
申请号：US12524557
申请日：2008-01-24
申请人： James A. Wells , Sami Mahrus
发明人： James A. Wells , Sami Mahrus
IPC分类号： G01N33/535
CPC分类号： G01N33/6803 , C12Q1/34
摘要： This invention provides general methods for selective labeling of proteins on their N-termini with synthetic peptides. The methods of this invention can be applied to the global proteomic profiling of complex mixtures of proteins and polypeptides.
摘要翻译： 本发明提供了用合成肽在其N-末端上选择性标记蛋白质的一般方法。 本发明的方法可以应用于蛋白质和多肽的复合物混合物的全局蛋白质组学分析。
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公开(公告)号：US20120328628A1
公开(公告)日：2012-12-27
申请号：US12307906
申请日：2007-07-06
申请人： James A. Wells , Junjun Gao
发明人： James A. Wells , Junjun Gao
IPC分类号： C07K1/00 , C07H21/04 , C07H21/02 , C07K16/00 , C07K2/00 , A61K38/02 , A61K39/395 , A61K31/7105 , A61K31/711 , C07K1/14 , A61K39/00 , A61P25/16 , A61P35/00 , A61P25/00 , A61P9/10 , A61P29/00 , A61P31/00 , C40B30/04 , G01N33/566 , C07H1/00
CPC分类号： G01N33/68 , C07K16/40 , C07K2299/00 , C07K2317/55 , C07K2317/565 , C07K2317/92 , G01N33/6845 , G01N33/6848 , G01N2500/00
摘要： The present invention provides methods for generating antibodies to specific conformations of proteins. The conformation specific antibodies of the invention can be put to a variety of uses including diagnosis and treatment of diseases and for screening for compounds that induce conformational changes in proteins upon binding.
摘要翻译： 本发明提供了产生针对特定蛋白质构象的抗体的方法。 本发明的构象特异性抗体可以用于各种用途，包括疾病的诊断和治疗以及用于筛选在结合时诱导蛋白质构象变化的化合物。
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公开(公告)号：US07375193B2
公开(公告)日：2008-05-20
申请号：US11537015
申请日：2006-09-29
IPC分类号： C07K16/00
CPC分类号： C07K16/22 , C07K2317/24 , C07K2317/55 , C07K2317/56 , C07K2317/565 , C07K2317/567 , C07K2317/73 , C07K2317/76 , C07K2317/92
摘要： Humanized and variant anti-VEGF antibodies and various uses therefor are disclosed. The anti-VEGF antibodies have strong binding affinities for VEGF; inhibit VEGF-induced proliferation of endothelial cells in vitro; and inhibit tumor growth in vivo.
摘要翻译： 公开了人源化和变异的抗VEGF抗体及其各种用途。 抗VEGF抗体对VEGF具有很强的结合亲和力; 体外抑制VEGF诱导的内皮细胞增殖; 并在体内抑制肿瘤生长。
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