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    Novel hydrazone-based and oxime-based fluorescent and chromophoric/pro-fluorescent and pro-chromophoric reagents and linkers
    1.
    发明申请
    Novel hydrazone-based and oxime-based fluorescent and chromophoric/pro-fluorescent and pro-chromophoric reagents and linkers 有权
    新型腙基和肟基荧光和发色/前荧光和原发色体试剂和接头

    公开(公告)号：US20080221343A1

    公开(公告)日：2008-09-11

    申请号：US11787932

    申请日：2007-04-18

    申请人： David A. Schwartz ,  Leopoldo Mendoza

    发明人： David A. Schwartz ,  Leopoldo Mendoza

    IPC分类号： C07D303/02 ,  C07C241/00

    CPC分类号： G01N33/582 ,  C07D303/02

    摘要： Conjugationally extended hydrazine compositions of the formula (RR2)N(H)n(NH2)n, fluorescent hydrazone compositions of the formula (RR2)NN═C(R1R2), methods of the formation of hydrazones from the reaction of conjugationally extended hydrazines with conjugationally extended carbonyls and methods of their use in assays systems are described. Use of these conjugationally extended hydrazine and oxime compositions for direct calorimetric and fluorometric assays wherein a chromophore or the fluorophore is incorporated into the linker that is positioned between a reactive linking moiety and a biotin molecule. More specifically the linker comprises one molecule of a high affinity binding pair such as for example biotin of the biotin/avidin high affinity binding pair, connected to a spacer molecule such as for example a length of polyethyleneglycol followed by a pro-chromophoric, chromophoric, pro-fluorescent or fluorescent moiety connected to an amino-, thiol- or carbohydrate-reactive moiety such as for example succinimidyl, maleimido or aminoxy group respectively, that may covalently link to a biomolecule.

    摘要翻译： 式（R 2 N）N（H）n（NH 2）n）的共轭延伸的肼组合物， 式（RR 2 N）NN-C（R 1 R 2 R 2）的荧光腙组合物，由反应形成腙的方法 描述了具有共轭延伸的羰基的共轭延伸的肼及其在测定系统中的用途。 使用这些共轭延伸的肼和肟组合物用于直接量热和荧光测定，其中发色团或荧光团被引入位于反应性连接部分和生物素分子之间的连接体中。 更具体地，接头包含一个高亲和力结合对分子，例如生物素/抗生物素蛋白高亲和力结合对的生物素，连接到间隔分子，例如长度的聚乙二醇，随后是发色团，发色团， 分别连接到可以共价连接到生物分子的氨基，硫醇或碳水化合物反应性部分的前荧光或荧光部分，例如琥珀酰亚胺基，马来酰亚胺基或氨氧基。

    Hydrazone-based and oxime-based fluorescent and chromophoric/pro-fluorescent and pro-chromophoric reagents and linkers
    2.
    发明授权
    Hydrazone-based and oxime-based fluorescent and chromophoric/pro-fluorescent and pro-chromophoric reagents and linkers 有权
    腙基和肟基荧光和发色/前荧光和原色发色试剂和接头

    公开(公告)号：US08541555B2

    公开(公告)日：2013-09-24

    申请号：US11787932

    申请日：2007-04-18

    申请人： David A. Schwartz ,  Leopoldo Mendoza

    发明人： David A. Schwartz ,  Leopoldo Mendoza

    IPC分类号： C07K16/00 ,  C07H21/04 ,  C07D235/02

    CPC分类号： G01N33/582 ,  C07D303/02

    摘要： Conjugationally extended hydrazine compositions of the formula (RR2)N(H)n(NH2)n, fluorescent hydrazone compositions of the formula (RR2)NN═C(R1R2), methods of the formation of hydrazones from the reaction of conjugationally extended hydrazines with conjugationally extended carbonyls and methods of their use in assays systems are described. Use of these conjugationally extended hydrazine and oxime compositions for direct calorimetric and fluorometric assays wherein a chromophore or the fluorophore is incorporated into the linker that is positioned between a reactive linking moiety and a biotin molecule. More specifically the linker comprises one molecule of a high affinity binding pair such as for example biotin of the biotin/avidin high affinity binding pair, connected to a spacer molecule such as for example a length of polyethyleneglycol followed by a pro-chromophoric, chromophoric, pro-fluorescent or fluorescent moiety connected to an amino-, thiol- or carbohydrate-reactive moiety such as for example succinimidyl, maleimido or aminoxy group respectively, that may covalently link to a biomolecule.

    摘要翻译： 式（RR2）N（H）n（NH2）n的共轭延伸的肼组合物，式（RR2）NN = C（R1R2）的荧光腙组合物，从共轭延伸的肼与 描述了缀合扩展的羰基及其在测定系统中的用途。 使用这些共轭延伸的肼和肟组合物用于直接量热和荧光测定，其中发色团或荧光团被引入位于反应性连接部分和生物素分子之间的连接体中。 更具体地，接头包含一个高亲和力结合对分子，例如生物素/抗生物素蛋白高亲和力结合对的生物素，连接到间隔分子，例如长度的聚乙二醇，随后是发色团，发色团， 分别连接到可以共价连接到生物分子的氨基，硫醇或碳水化合物反应性部分的前荧光或荧光部分，例如琥珀酰亚胺基，马来酰亚胺基或氨氧基。

    Methods and/or Use of Oligonucleotide Conjugates for Assays and Microscopy/Imaging Detections
    5.
    发明申请
    Methods and/or Use of Oligonucleotide Conjugates for Assays and Microscopy/Imaging Detections 审中-公开
    用于测定和显微镜/成像检测的寡核苷酸缀合物的方法和/或用途

    公开(公告)号：US20120258881A1

    公开(公告)日：2012-10-11

    申请号：US13302890

    申请日：2011-11-22

    申请人： David A. Schwartz ,  Jimmy Williams ,  Xinfang Zhao ,  Chunfang Zhao ,  William B. Busa ,  Stephen J. Kron ,  Amy Catherine Flor

    发明人： David A. Schwartz ,  Jimmy Williams ,  Xinfang Zhao ,  Chunfang Zhao ,  William B. Busa ,  Stephen J. Kron ,  Amy Catherine Flor

    IPC分类号： C12Q1/68 ,  G01N21/76 ,  G01N21/00 ,  G01N21/59 ,  G01N21/47 ,  G01N21/64 ,  G01N21/25 ,  G01N21/66 ,  G01N21/65 ,  G01N24/00 ,  G01N27/72 ,  C40B30/04 ,  C12Q1/70 ,  B82Y15/00

    CPC分类号： C12Q1/6804 ,  C07H21/00 ,  C12Q1/6834 ,  C12Q2565/113

    摘要： The present disclosure is directed to methods and/or uses of oligonucleotide conjugates for assays and microscopy/imaging detections and related systems and/or kits. Certain methods are directed to a method for detecting one or more biological targets of a sample in a detection assay, comprising: providing a molecular probe, comprising a binding moiety and an oligonucleotide sequence, to a sample comprising one or more biological targets; binding the one or more biological targets with the binding moiety; providing a detectable component to the sample, wherein the detectable component comprises a signal generating moiety conjugated to an oligonucleotide sequence complementary to the oligonucleotide sequence of the molecular probe; hydridizing the oligonucleotide sequence of the target-bound molecular probe to the detectable component; and detecting a signal generated from the hydridized detectable component. Various other embodiments, applications etc. are disclosed herein.

    摘要翻译： 本公开涉及用于测定和显微镜/成像检测和相关系统和/或试剂盒的寡核苷酸缀合物的方法和/或用途。 某些方法涉及用于在检测测定中检测样品的一种或多种生物学靶标的方法，其包括：向包含一种或多种生物靶标的样品提供包含结合部分和寡核苷酸序列的分子探针; 将一种或多种生物靶与结合部分结合; 向所述样品提供可检测组分，其中所述可检测组分包含与与所述分子探针的寡核苷酸序列互补的寡核苷酸序列缀合的信号产生部分; 将靶结合分子探针的寡核苷酸序列杂交到可检测组分; 以及检测由所述可混合的可检测组分产生的信号。 本文公开了各种其它实施例，应用等。

    Animal model for detection of vulnerable plaques
    7.
    发明授权
    Animal model for detection of vulnerable plaques 失效
    用于检测脆弱斑块的动物模型

    公开(公告)号：US06580016B2

    公开(公告)日：2003-06-17

    申请号：US09760182

    申请日：2001-01-12

    申请人： Paul S. Teirstein ,  Kenneth W. Carpenter ,  Istvan Szinai ,  David A. Schwartz

    发明人： Paul S. Teirstein ,  Kenneth W. Carpenter ,  Istvan Szinai ,  David A. Schwartz

    IPC分类号： A01K6700

    CPC分类号： A61K49/126 ,  A61B6/4057 ,  A61B6/481 ,  A61B6/508 ,  A61K49/0442 ,  A61K49/085 ,  A61K51/065 ,  G01N33/92 ,  G01N2400/40 ,  G01N2800/323

    摘要： The present invention provides in vivo methods for detection of vulnerable plaque in a subject in need thereof. In the invention method the subject is administered a diagnostic amount of a biologically compatible detectable lipid-avid agent, the detectable lipid-avid agent is allowed to penetrate arterial walls and attach to any lipid accumulations of oxidized LDL-cholesterol in arterial walls in the wall of an artery; unbound detectable lipid-avid agent is allowed to clear from the body by natural processes, and the presence of the detectable lipid-avid agent attached to the lipid accumulation in the wall of the artery is detected. Detection of bound lipid-avid agent indicates the presence of a vulnerable plaque and predicts a heightened risk of lethal heart attack or thrombus. The detectable lipid-avid agent is selected for its ability to penetrate arterial walls and bind with oxidized LDL-cholesterol in the lipid accumulation in a vulnerable plaque. Alternatively, macrophage-avid agents, for example a lipid-avid agent attached to a macrophage-specific antibody, can be used in the invention methods. The invention further includes methods for in vitro assays for detecting vulnerable plaque and a porcine animal model of vulnerable plaque useful for testing treatment modalities.

    摘要翻译： 本发明提供了在有需要的受试者中检测易损斑块的体内方法。 在本发明方法中，给予受试者诊断量的生物相容性可检测的脂质 - 抗体试剂，允许可检测的脂质激动剂渗透动脉壁并附着于壁中动脉壁中氧化的LDL-胆固醇的任何脂质积聚 的动脉 通过天然过程使未结合的可检测的脂质 - 抗体试剂从身体中清除，并且检测附着在动脉壁中的脂质积聚的可检测的脂质激动剂的存在。 检测结合的脂质抗体剂表明存在易受伤害的斑块，并预测致死性心脏病发作或血栓的风险增加。 选择可检测的脂质抗体试剂，以其穿透动脉壁并与氧化的LDL-胆固醇结合在易损斑块中的脂质积聚中。 或者，巨噬细胞激动剂，例如与巨噬细胞特异性抗体连接的脂质 - 抗体试剂可用于本发明的方法中。 本发明还包括用于检测易损斑块的体外测定方法和用于测试治疗方式的易损斑块的猪动物模型。

    Wide memory architecture vector processor using nxP bits wide memory bus for transferring P n-bit vector operands in one cycle
    8.
    发明授权
    Wide memory architecture vector processor using nxP bits wide memory bus for transferring P n-bit vector operands in one cycle 失效
    宽存储器架构矢量处理器，使用nxP位宽的存储器总线，用于在一个周期内传输P n位向量操作数

    公开(公告)号：US5928350A

    公开(公告)日：1999-07-27

    申请号：US840178

    申请日：1997-04-11

    申请人： David B. Shu ,  David A. Schwartz ,  Lap-Wai Chow

    发明人： David B. Shu ,  David A. Schwartz ,  Lap-Wai Chow

    IPC分类号： G06F9/30 ,  G06F9/38 ,  G06F15/78 ,  G06F9/312

    CPC分类号： G06F15/8069 ,  G06F9/30141 ,  G06F9/3826 ,  G06F9/3885 ,  G06F9/3887

    摘要： A wide memory architecture is provided for storing data associated with a vector processor. Additionally, a method for accessing a wide memory architecture is provided. The wide memory architecture includes a memory for storing an array of vector operands. The memory is coupled to a data bus which provides an access pathway connecting the memory to a processor. The wide memory architecture further includes at least one staging buffer disposed between the memory and the processor. The staging buffer is capable of providing intermediate storage of a vector operand upon which a function can be performed by the processor.

    摘要翻译： 提供了用于存储与矢量处理器相关联的数据的宽存储器架构。 另外，提供了用于访问宽存储器架构的方法。 宽存储器架构包括用于存储向量操作数的阵列的存储器。 存储器耦合到数据总线，数据总线提供将存储器连接到处理器的访问路径。 宽的存储器架构还包括设置在存储器和处理器之间的至少一个分级缓冲器。 分段缓冲器能够提供向量操作数的中间存储器，由此可以由处理器执行功能。

    Computer processing element having first and second functional units accessing shared memory output port on prioritized basis
    9.
    发明授权
    Computer processing element having first and second functional units accessing shared memory output port on prioritized basis 失效
    计算机处理元件具有第一和第二功能单元，以优先级为基础访问共享存储器输出端口

    公开(公告)号：US5481736A

    公开(公告)日：1996-01-02

    申请号：US018683

    申请日：1993-02-17

    申请人： David A. Schwartz ,  James J. Radigan

    发明人： David A. Schwartz ,  James J. Radigan

    IPC分类号： G06F9/38 ,  G06F9/30

    CPC分类号： G06F9/3885

    摘要： A processing element (42) design is provided for improving performance and reducing the number (30') of memory ports by eliminating the dedication of ports to specific functional units (22, 24, 26, 28) and by providing data paths (46, 48, 50, 52) to other forward results from functional unit outputs directly to other functional unit inputs.

    摘要翻译： 提供了一种处理元件（42）设计，用于通过消除对特定功能单元（22,24,26,28）的端口的贡献来提高性能并减少存储器端口的数量（30'），并且通过提供数据路径（46， 48,50,52）将功能单元输出的其他前向结果直接传送到其他功能单元输入。