-
公开(公告)号：US5599907A
公开(公告)日：1997-02-04
申请号：US240712
申请日：1994-05-09
IPC分类号： A61K38/00 , A61K47/48 , C07K14/805 , C12N15/12 , C12N15/81 , A61K35/14 , C07H21/04 , C12P21/00
CPC分类号： C12N15/81 , A61K47/48307 , C07K14/805 , A61K38/00 , C07K2319/00 , C07K2319/50 , C07K2319/92 , Y10S530/829
摘要： Multimeric hemoglobin-like proteins are obtained by crosslinking cysteines of the component tetramers, or by genetically fusing globin-like domains of one tetramer with those of another, by means of an interdomain spacer sequence. Artificial cysteines are introduced selectively in a single globin-like domain per tetramer to control polymerization.
摘要翻译： PCT No.PCT / US92 / 09752 Sec。 371日期1994年5月9日 102（e）日期1994年5月9日PCT提交1992年11月6日PCT公布。 公开号WO93 / 09143 日期1993年5月13日多聚体血红蛋白样蛋白质通过交联组分四聚体的半胱氨酸，或通过间隔区间序列通过将一个四聚体的球蛋白样结构域与另一个四聚体的球蛋白样结构域交联而获得。 人造半胱氨酸被选择性地引入单个珠蛋白样结构域每四聚体以控制聚合。
-
公开(公告)号：US06184356B2
公开(公告)日：2001-02-06
申请号：US09058562
申请日：1998-04-13
IPC分类号： C07K14805
CPC分类号： C12N15/81 , A61K38/00 , A61K47/6445 , C07K14/805 , C07K2319/00 , C07K2319/50 , C07K2319/92 , Y10S530/829
摘要： DNA molecules which encode pseudodimeric globin-like polypeptides with an asymmetric cysteine mutation suitable for crosslinking two tetramers, or which encode pseudooligomeric globin-like polypeptides comprising four or more globin-like domains, are useful in the preparation of multimeric hemoglobin-like proteins.
摘要翻译： 编码具有适用于交联两个四聚体的不对称半胱氨酸突变的伪二聚球蛋白样多肽的DNA分子或编码包含四个或更多个珠蛋白样结构域的假寡聚寡核苷酸样多肽的DNA分子可用于制备多聚体血红蛋白样蛋白。
-
公开(公告)号：US5844088A
公开(公告)日：1998-12-01
申请号：US444991
申请日：1995-05-19
申请人： Stephen J. Hoffman , Douglas L. Looker , Mary S. Rosendahl , Gary L. Stetler , Michael Wagenbach , David C. Anderson , Antony James Mathews , Kiyoshi Nagai
发明人： Stephen J. Hoffman , Douglas L. Looker , Mary S. Rosendahl , Gary L. Stetler , Michael Wagenbach , David C. Anderson , Antony James Mathews , Kiyoshi Nagai
IPC分类号： A61K38/16 , A61K38/00 , A61K47/48 , A61P7/08 , C07K20060101 , C07K1/113 , C07K14/805 , C12N15/00 , C12N15/09 , C12N15/12 , C12N15/81 , C12P21/02 , C12R1/19 , C12R1/91
CPC分类号： C07K14/805 , A61K47/48307 , C12N15/81 , A61K38/00 , C07K2319/00 , C07K2319/50 , C07K2319/92
摘要： The alpha subunits of hemoglobin, which in nature are formed as separate polypeptide chains which bind noncovalently to the beta subunits, are here provided in the form of the novel molecule di-alpha globin, a single polypeptide chain defined by connecting the two alpha subunits either directly via peptide bond or indirectly by a flexible amino acid or peptide linker. Di-alpha globin may be combined in vivo or in vitro with beta globin and heme to form hemoglobin. Di-alpha globin is expressed by recombinant DNA techniques. Di-beta globin may be similarly obtained. We further describe the production of tetrameric human hemoglobin and di-alpha/beta.sub.2 hemoglobin in the yeast Saccharomyces cerevisiae. The synthesis of the protein is directed by a synthetic promotor consisting of two functional parts, an upstream activator sequence (UAS) that confers inducible transcription by galactose from a consensus yeast transcriptional initiation site. The expression construct is designed such that translation is expected to initiate at the same position as the human wild-type genes for .alpha.- and .beta.-globin. Three different types of expression vectors have been used: (1) .alpha.-globin and .beta.-globin contained on two separate plasmids (pGS4688 and pGS4988) in a diploid yeast strain; (2) .alpha.-globin and .beta.-globin each contained on a single plasmid (pGS289 and pGS389) and expressed in either haploid or diploid strains; and (3) di-alpha-globin and beta globin contained on a single plasmid (pGS 3089)and expressed in haploid strains. Finally, we describe the co-expression of alpha and beta globin chains. The chains are folded together and combined intracellularly with heme to form active tetrameric hemoglobin. The hemoglobin may be recovered from the cells' soluble fraction. The invention thus obviates the need to express alpha and beta globin separately, solubilize, renature and purify them, and combine them in vitro with heme to obtain an artificial hemoglobin. By way of comparison, the separately expressed beta globin known in the art is deposited in inclusion bodies. Polycistronic co-expression of alpha and beta globins is particularly preferred.
-
4.发明授权DNA encoding fused di-beta globins and production of pseudotetrameric hemoglobin 失效编码融合的二-β珠蛋白的DNA和伪三聚体血红蛋白的产生
公开(公告)号：US5744329A
公开(公告)日：1998-04-28
申请号：US444942
申请日：1995-05-19
申请人： Stephen J. Hoffman , Douglas L. Looker , Mary S. Rosendahl , Gary L. Stetler , Michael Wagenbach , David C. Anderson , Antony James Mathews , Kiyoshi Nagai
发明人： Stephen J. Hoffman , Douglas L. Looker , Mary S. Rosendahl , Gary L. Stetler , Michael Wagenbach , David C. Anderson , Antony James Mathews , Kiyoshi Nagai
IPC分类号： A61K38/16 , A61K38/00 , A61K47/48 , A61P7/08 , C07K20060101 , C07K1/113 , C07K14/805 , C12N15/00 , C12N15/09 , C12N15/12 , C12N15/81 , C12P21/02 , C12R1/19 , C12R1/91 , C12P21/06 , C07H17/00
CPC分类号： C07K14/805 , A61K47/48307 , C12N15/81 , A61K38/00 , C07K2319/00 , C07K2319/50 , C07K2319/92
摘要： The alpha subunits of hemoglobin, which in nature are formed as separate polypeptide chains which bind noncovalently to the beta subunits, are here provided in the form of the novel molecule di-alpha globin, a single polypeptide chain defined by connecting the two alpha subunits either directly via peptide bond or indirectly by a flexible amino acid or peptide linker. Di-alpha globin may be combined in vivo or in vitro with beta globin and heme to form hemoglobin. Di-alpha globin is expressed by recombinant DNA techniques. Di-beta globin may be similarly obtained.
摘要翻译： 在本文中，血红蛋白的α亚基本质上被形成为与β-亚基非共价结合的分离的多肽链，这里以新分子二-α球蛋白的形式提供，单链多肽链通过将两个α亚基连接而定义 直接通过肽键或通过柔性氨基酸或肽接头间接。 Di-α珠蛋白可以在体内或体外与β球蛋白和血红素组合以形成血红蛋白。 Di-α球蛋白通过重组DNA技术表达。 可以类似地获得二β-珠蛋白。
-
公开(公告)号：US5739011A
公开(公告)日：1998-04-14
申请号：US443890
申请日：1995-05-31
CPC分类号： C12N15/81 , A61K47/48307 , C07K14/805 , A61K38/00 , C07K2319/00 , C07K2319/50 , C07K2319/92 , Y10S530/829
摘要： DNA molecules which encode pseudodimeric globin-like polypeptides with an asymmetric cysteine mutation suitable for crosslinking two tetramers, or which encode pseudooligomeric globin-like polypeptides comprising four or more globin-like domains, are useful in the preparation of multimeric hemoglobin-like proteins.
摘要翻译： 编码具有适用于交联两个四聚体的不对称半胱氨酸突变的伪二聚球蛋白样多肽的DNA分子或编码包含四个或更多个珠蛋白样结构域的假寡聚寡核苷酸样多肽的DNA分子可用于制备多聚体血红蛋白样蛋白。
-
6.发明授权
公开(公告)号：US5844089A
公开(公告)日：1998-12-01
申请号：US450733
申请日：1995-05-25
申请人： Stephen J. Hoffman , Douglas L. Looker , Mary S. Rosendahl , Gary L. Stetler , Michael Wagenbach , David C. Anderson , Antony James Mathews , Kiyoshi Nagai
发明人： Stephen J. Hoffman , Douglas L. Looker , Mary S. Rosendahl , Gary L. Stetler , Michael Wagenbach , David C. Anderson , Antony James Mathews , Kiyoshi Nagai
IPC分类号： A61K38/16 , A61K38/00 , A61K47/48 , A61P7/08 , C07K20060101 , C07K1/113 , C07K14/805 , C12N15/00 , C12N15/09 , C12N15/12 , C12N15/81 , C12P21/02 , C12R1/19 , C12R1/91
CPC分类号： C07K14/805 , A61K47/48307 , C12N15/81 , A61K38/00 , C07K2319/00 , C07K2319/50 , C07K2319/92
摘要： The alpha subunits of hemoglobin, which in nature are formed as separate polypeptide chains which bind noncovalently to the beta subunits, are here provided in the form of the novel molecule di-alpha globin, a single polypeptide chain defined by connecting the two alpha subunits either directly via peptide bond or indirectly by a flexible amino acid or peptide linker. Di-alpha globin may be combined in vivo or in vitro with beta globin and heme to form hemoglobin. Di-alpha globin is expressed by recombinant DNA techniques. Di-beta globin may be similarly obtained.
摘要翻译： 在本文中，血红蛋白的α亚基本质上被形成为与β-亚基非共价结合的分离的多肽链，这里以新分子二-α球蛋白的形式提供，单链多肽链通过将两个α亚基连接而定义 直接通过肽键或通过柔性氨基酸或肽接头间接。 Di-α珠蛋白可以在体内或体外与β球蛋白和血红素组合以形成血红蛋白。 Di-α球蛋白通过重组DNA技术表达。 可以类似地获得二β-珠蛋白。
-
公开(公告)号：US5717058A
公开(公告)日：1998-02-10
申请号：US199508
申请日：1994-02-18
IPC分类号： A61K38/00 , A61K48/00 , C07K14/02 , C07K14/15 , C12N15/67 , C07K14/155 , C07K4/02 , C07K5/00 , C07K7/00
CPC分类号： C07K14/005 , C12N15/67 , A01K2217/05 , A61K38/00 , A61K48/00 , C07K2319/00 , C12N2730/10122 , C12N2740/14022
摘要： The present invention relates to regulators of cellular gene transcription, particularly inhibitors of cellular gene transactivating factors and in particular to inhibition of gene transcription in a viral host cell that is subject to regulation by proteins or factors that originate from a virus as well as conjugates or fusion products of the inhibitors and internalization molecules, pharmaceutical compositions that can be used to alleviate or prevent the manifestation of disease states that are the result of unregulated DNA transcription as a result of transactivation, methods of treating diseases that are caused or exacerbated by the presence of transactivating factors, and regulated gene therapy to achieve long term drug delivery of the inhibitors of the present invention. This invention can be applied both to cells with genetic abnormalities or to cells infected with a virus. Preferably, at least one protein of the protein-protein interactions is a transactivating factor.
摘要翻译： 本发明涉及细胞基因转录的调节剂，特别是细胞基因反式激活因子的抑制剂，特别是抑制病毒宿主细胞中的基因转录，所述基因转录受来自病毒的蛋白质或因子的调节，以及缀合物或 抑制剂和内化分子的融合产物，可用于缓解或预防作为反式激活导致的不受调节的DNA转录的结果的疾病状态的表现的药物组合物，治疗由于存在引起或加剧的疾病的方法 的转录因子和调节基因治疗以实现本发明的抑制剂的长期药物递送。 本发明可以应用于具有遗传异常的细胞或用于感染病毒的细胞。 优选地，蛋白质 - 蛋白质相互作用的至少一种蛋白质是反式激活因子。
-
公开(公告)号：US6150506A
公开(公告)日：2000-11-21
申请号：US973629
申请日：1998-08-24
申请人： Stephen P. Trimble , Antony J. Mathews , Bruce A. Kerwin , David A. Marquardt , Spencer Anthony-Cahill , Janet K. Epp , Dominic G. Madril , David C. Anderson
发明人： Stephen P. Trimble , Antony J. Mathews , Bruce A. Kerwin , David A. Marquardt , Spencer Anthony-Cahill , Janet K. Epp , Dominic G. Madril , David C. Anderson
IPC分类号： A61K38/00 , A61K47/48 , C07K14/805 , C12N15/81 , A61K35/14
CPC分类号： C12N15/81 , A61K47/48307 , C07K14/805 , A61K38/00 , C07K2319/00
摘要： The present invention relates to modified hemoglobin-like polypeptides containing multiple dialpha (or dibeta) domains. The present invention also relates to multimeric hemoglobin-like proteins comprising covalently joined hemoglobin-like moieties. Another aspect of the inention is directed at a purification method of hemoglobin-like polypeptides utilizing ion exchange chromatography.
摘要翻译： PCT No.PCT / US96 / 10420 Sec。 371日期：1998年8月24日 102（e）1998年8月24日PCT PCT 1996年6月6日PCT公布。 公开号WO96 / 40920 PCT 日期：1996年12月19日本发明涉及含有多个（或二）β域的修饰的血红蛋白样多肽。 本发明还涉及包含共价连接的血红蛋白样部分的多聚体血红蛋白样蛋白。 本发明的另一方面涉及使用离子交换层析的血红蛋白样多肽的纯化方法。
-
公开(公告)号：US5844090A
公开(公告)日：1998-12-01
申请号：US487431
申请日：1995-06-07
IPC分类号： C12N15/09 , A61K38/00 , A61K38/16 , A61K38/42 , A61P7/08 , C07K1/18 , C07K14/805 , C12N15/12 , A61K35/14 , C12P21/02
CPC分类号： C07K14/805 , A61K38/00 , C07K2319/00 , Y10S530/829
摘要： The present invention relates to modified hemoglobin-like compounds. The novel compounds include a globin-like polypeptide containing at least two di-alpha domains and multimeric hemoglobin-like proteins having a core hemoglobin-like moiety directly attached to at least two other hemoglobin-like moieties. The invention also relates to nucleic acid molecules encoding the novel polypeptides. Methods of making the multimeric hemoglobin-like proteins are also provided, as well as compositions containing the proteins.
摘要翻译： 本发明涉及修饰的血红蛋白样化合物。 新化合物包括含有至少两个二-α结构域的球蛋白样多肽和具有直接连接至至少两个其它血红蛋白样部分的核心血红蛋白样部分的多聚体血红蛋白样蛋白。 本发明还涉及编码新型多肽的核酸分子。 还提供了制备多聚体血红蛋白样蛋白的方法，以及含有蛋白质的组合物。
-
公开(公告)号：US07425418B2
公开(公告)日：2008-09-16
申请号：US10098243
申请日：2002-03-15
IPC分类号： G01N33/53
CPC分类号： C40B30/04 , G01N33/566 , G01N33/6845 , G01N33/6875 , G01N2333/70567 , G06F19/18
摘要： The present provides compounds capable of modulating IL-4 receptor-mediated IgE production, as well as IL-4 induced processes associated therewith, methods and kits for identifying such compounds that utilize a retinoid X receptor as a surrogate analyte and methods of using the compounds in a variety of in vitro, in vitro and ex vivo contexts.
摘要翻译： 本发明提供了能够调节IL-4受体介导的IgE产生的化合物以及与其相关的IL-4诱导的过程，用于鉴定利用类视黄醇X受体作为替代分析物的化合物的方法和试剂盒以及使用该化合物的方法 在各种体外，体外和体外上下文中。
-
-
-
-
-
-
-
-
-
搜索结果
41 条记录 (耗时 0.036 秒)