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    Methods, compositions, and kits for detecting protein aggregates
    1.
    发明授权
    Methods, compositions, and kits for detecting protein aggregates 有权
    用于检测蛋白质聚集体的方法，组合物和试剂盒

    公开(公告)号：US08535878B2

    公开(公告)日：2013-09-17

    申请号：US11743517

    申请日：2007-05-02

    申请人： David W. Ruff ,  Mark E. Shannon ,  Kenneth J. Livak ,  Karl J. Guegler ,  Kevin M. Hennessy

    发明人： David W. Ruff ,  Mark E. Shannon ,  Kenneth J. Livak ,  Karl J. Guegler ,  Kevin M. Hennessy

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6876 ,  C07K14/47 ,  C07K14/4711 ,  C12Q1/6816 ,  C12Q2533/107

    摘要： The present teachings provide methods, compositions, and kits for detecting the presence of protein aggregates. In some embodiments, the protein aggregate is treated with a labeled precursor, and the labeled precursor is incorporated into the protein aggregate to form a labeled protein aggregate. The labeled protein aggregate is then measured, thus detecting the presence of the protein aggregate. In some embodiments, the labeled protein aggregate is detected by interaction of labeled precursors, for example by a proximity ligation assay.

    摘要翻译： 本教导提供了用于检测蛋白质聚集体的存在的方法，组合物和试剂盒。 在一些实施方案中，用标记的前体处理蛋白质聚集体，并将标记的前体掺入蛋白质聚集体中以形成标记的蛋白质聚集体。 然后测量标记的蛋白质聚集体，从而检测蛋白质聚集体的存在。 在一些实施方案中，标记的蛋白质聚集体通过标记的前体的相互作用来检测，例如通过邻近连接测定。

    Systems and methods for isolating nucleic acids
    2.
    发明授权
    Systems and methods for isolating nucleic acids 失效
    用于分离核酸的系统和方法

    公开(公告)号：US08568580B2

    公开(公告)日：2013-10-29

    申请号：US12179455

    申请日：2008-07-24

    申请人： Charles S. Vann ,  Maxim G. Brevnov ,  David W. Ruff ,  Kenneth J. Livak

    发明人： Charles S. Vann ,  Maxim G. Brevnov ,  David W. Ruff ,  Kenneth J. Livak

    IPC分类号： G01N27/453 ,  B01D61/42

    CPC分类号： C12N15/101 ,  G01N27/44782

    摘要： A system for collecting target nucleic acids from a sample can include at least one sample chamber configured to receive a sample containing target nucleic acids and other material, at least one collection chamber removably mountable relative to the at least one sample chamber and configured to collect target nucleic acids separated from the other material, a filter removably mountable relative to the at least one sample chamber and configured to be disposed between the at least one sample chamber and the at least one collection chamber when the at least one collection chamber is mounted relative to the at least one sample chamber. The system may further include a pair of electrodes configured to generate an electric field sufficient to cause target nucleic acids in the at least one sample chamber to migrate via electrophoresis from the at least one sample chamber through the filter into the at least one collection chamber, wherein the filter may be configured to permit passage of target nucleic acids and to block passage of material of a size larger than the target nucleic acids.

    摘要翻译： 用于从样品收集目标核酸的系统可以包括至少一个样品室，其被配置为接收含有靶核酸和其它材料的样品，至少一个收集室，其相对于至少一个样品室可拆卸地安装并且被配置成收集目标 与其他材料分离的核酸，相对于至少一个样品室可拆卸地安装的过滤器，并且被配置为当所述至少一个收集室相对于所述至少一个收集室安装时，被设置在所述至少一个样品室和所述至少一个收集室之间 所述至少一个样品室。 该系统可以进一步包括一对电极，其被配置为产生足以使所述至少一个样品室中的靶核酸经由电泳从所述至少一个样品室通过所述过滤器迁移到所述至少一个收集室中的电场， 其中所述过滤器可以被配置为允许靶核酸的通过并阻止尺寸大于所述靶核酸的材料的通过。

    Systems and Methods for Isolating Nucleic Acids
    3.
    发明申请
    Systems and Methods for Isolating Nucleic Acids 失效
    用于分离核酸的系统和方法

    公开(公告)号：US20090071830A1

    公开(公告)日：2009-03-19

    申请号：US12179455

    申请日：2008-07-24

    申请人： Charles S. Vann ,  Maxim G. Brevnov ,  David W. Ruff ,  Kenneth J. Livak

    发明人： Charles S. Vann ,  Maxim G. Brevnov ,  David W. Ruff ,  Kenneth J. Livak

    IPC分类号： B01D57/02 ,  B01D61/42 ,  C25B9/08

    CPC分类号： C12N15/101 ,  G01N27/44782

    摘要： A system for collecting target nucleic acids from a sample can include at least one sample chamber configured to receive a sample containing target nucleic acids and other material, at least one collection chamber removably mountable relative to the at least one sample chamber and configured to collect target nucleic acids separated from the other material, a filter removably mountable relative to the at least one sample chamber and configured to be disposed between the at least one sample chamber and the at least one collection chamber when the at least one collection chamber is mounted relative to the at least one sample chamber. The system may further include a pair of electrodes configured to generate an electric field sufficient to cause target nucleic acids in the at least one sample chamber to migrate via electrophoresis from the at least one sample chamber through the filter into the at least one collection chamber, wherein the filter may be configured to permit passage of target nucleic acids and to block passage of material of a size larger than the target nucleic acids.

    摘要翻译： 用于从样品收集目标核酸的系统可以包括至少一个样品室，其被配置为接收含有靶核酸和其它材料的样品，至少一个收集室，其相对于至少一个样品室可拆卸地安装并且被配置成收集目标 与其他材料分离的核酸，相对于至少一个样品室可拆卸地安装的过滤器，并且被配置为当所述至少一个收集室相对于所述至少一个收集室安装时，被设置在所述至少一个样品室和所述至少一个收集室之间 所述至少一个样品室。 该系统可以进一步包括一对电极，其被配置为产生足以使所述至少一个样品室中的靶核酸经由电泳从所述至少一个样品室通过所述过滤器迁移到所述至少一个收集室中的电场， 其中所述过滤器可以被配置为允许靶核酸的通过并阻止尺寸大于所述靶核酸的材料的通过。

    Normalization of Gene Expression Data
    4.
    发明申请
    Normalization of Gene Expression Data 审中-公开
    基因表达数据的归一化

    公开(公告)号：US20100036616A1

    公开(公告)日：2010-02-11

    申请号：US12558428

    申请日：2009-09-11

    申请人： Mark E. Shannon ,  Mark F. Oldham ,  David W. Ruff

    发明人： Mark E. Shannon ,  Mark F. Oldham ,  David W. Ruff

    IPC分类号： G06F19/00

    CPC分类号： G06F19/20 ,  C12Q1/686 ,  C40B30/04

    摘要： A method for determining bias across two domains comprising gene expression data. The method can comprise (a) providing a first domain and a second domain; (b) obtaining information indicative of a bias within the first domain; (c) obtaining information indicative of a bias within the second domain; and (d) using the information indicative of the bias within the first domain and the information indicative of the bias within the second domain to produce an indication of bias across the two domains.

    摘要翻译： 一种用于确定包含基因表达数据的两个结构域之间的偏差的方法。 该方法可以包括（a）提供第一域和第二域; （b）获得指示第一域内偏倚的信息; （c）获得指示第二域内偏倚的信息; 以及（d）使用指示第一域内的偏差的信息和指示第二域内的偏差的信息来产生跨越两个域的偏差的指示。

    Method for cross-instrument comparison of gene expression data
    5.
    发明授权
    Method for cross-instrument comparison of gene expression data 有权
    交叉仪器比较基因表达数据的方法

    公开(公告)号：US09026371B2

    公开(公告)日：2015-05-05

    申请号：US12558428

    申请日：2009-09-11

    申请人： Mark E. Shannon ,  Mark F. Oldham ,  David W. Ruff

    发明人： Mark E. Shannon ,  Mark F. Oldham ,  David W. Ruff

    IPC分类号： G01N33/48 ,  C40B30/04 ,  G06F19/20 ,  C12Q1/68

    CPC分类号： G06F19/20 ,  C12Q1/686 ,  C40B30/04

    摘要： A method for determining bias across two domains comprising gene expression data. The method can comprise (a) providing a first domain and a second domain; (b) obtaining information indicative of a bias within the first domain; (c) obtaining information indicative of a bias within the second domain; and (d) using the information indicative of the bias within the first domain and the information indicative of the bias within the second domain to produce an indication of bias across the two domains.

    摘要翻译： 一种用于确定包含基因表达数据的两个结构域之间的偏差的方法。 该方法可以包括（a）提供第一域和第二域; （b）获得指示第一域内偏倚的信息; （c）获得指示第二域内偏倚的信息; 以及（d）使用指示第一域内的偏差的信息和指示第二域内的偏差的信息来产生跨越两个域的偏差的指示。

    Methods for the analysis of proximity binding assay data
    8.
    发明授权
    Methods for the analysis of proximity binding assay data 有权

    公开(公告)号：US10208335B2

    公开(公告)日：2019-02-19

    申请号：US12851532

    申请日：2010-08-05

    申请人： Shiaw-Min Chen ,  David W. Ruff ,  Harrison M. Leong

    发明人： Shiaw-Min Chen ,  David W. Ruff ,  Harrison M. Leong

    IPC分类号： G06F19/24 ,  G06F19/00 ,  G06F17/00 ,  C12Q1/6844 ,  G06F19/20

    摘要： Various embodiments of methods for analyzing proximity binding assay (PBA) data are disclosed. Proximity binding assays as a class of analyses offer the advantages of the sensitivity and specificity of biorecognition binding, along with the exponential signal amplification offered by a variety of oligonucleotide amplification reactions, such as the polymerase chain reaction (PCR). However, as various proximity binding assays have reaction kinetics governed by an additional step of the binding of a biorecognition probe (BRP) with a target molecule, there is a need for methods for the analysis of PBA data that are particularly suited to the unique characteristics of such data.

    Methods for the Analysis of Proximity Binding Assay Data
    10.
    发明申请
    Methods for the Analysis of Proximity Binding Assay Data 审中-公开
    接近结合测定数据分析方法

    公开(公告)号：US20110208441A1

    公开(公告)日：2011-08-25

    申请号：US12851532

    申请日：2010-08-05

    申请人： Shiaw-Min Chen ,  David W. Ruff ,  Harrison M. Leong

    发明人： Shiaw-Min Chen ,  David W. Ruff ,  Harrison M. Leong

    IPC分类号： G06F19/00

    CPC分类号： C12Q1/6844 ,  G06F19/20 ,  G06F19/24 ,  C12Q2545/114

    摘要： Various embodiments of methods for analyzing proximity binding assay (PBA) data are disclosed. Proximity binding assays as a class of analyses offer the advantages of the sensitivity and specificity of biorecognition binding, along with the exponential signal amplification offered by a variety of oligonucleotide amplification reactions, such as the polymerase chain reaction (PCR). However, as various proximity binding assays have reaction kinetics governed by an additional step of the binding of a biorecognition probe (BRP) with a target molecule, there is a need for methods for the analysis of PBA data that are particularly suited to the unique characteristics of such data.

    摘要翻译： 公开了用于分析邻近结合测定（PBA）数据的方法的各种实施方案。 作为一类分析的接近结合测定提供了生物识别结合的灵敏度和特异性以及由多种寡核苷酸扩增反应（例如聚合酶链反应（PCR））提供的指数信号扩增的优点。 然而，由于各种邻近结合测定具有由生物识别探针（BRP）与靶分子结合的附加步骤所决定的反应动力学，因此需要分析特别适合于独特特征的PBA数据的方法 的数据。