公开(公告)号：US06935617B2

公开(公告)日：2005-08-30

申请号：US10625449

申请日：2003-07-23

申请人： Dennis E. Mead ,  Zbigniew T. Bryning

发明人： Dennis E. Mead ,  Zbigniew T. Bryning

IPC分类号： G01N37/00 ,  B01J19/00 ,  B01L3/00 ,  B01L3/02 ,  B81B3/00 ,  F16K1/00 ,  F16K1/10 ,  F16K13/10 ,  F16K31/02 ,  G01N1/10

CPC分类号： F16K99/0001 ,  B01L3/502738 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/10 ,  B01L2300/0803 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0409 ,  B01L2400/0487 ,  B01L2400/0655 ,  B01L2400/0683 ,  F16K99/0026 ,  F16K99/0034 ,  F16K99/0036 ,  F16K2099/0069 ,  F16K2099/0084

摘要： A normally open fluid manipulation valve assembly and system and a method for closing, re-opening and re-closing same. The normally open valve assembly can include a substrate including a first surface, with first and second recesses formed in the first surface. A recessed channel can be formed in the first surface. The recessed channel can extend from the first recess to the second recess and can be at least partially defined by a first deformable material having a first modulus of elasticity. The valve assembly can also include an elastically deformable cover. The elastically deformable cover can include a layer of an elastically deformable material having a modulus of elasticity that is greater than the modulus of elasticity of the first deformable material, and an adhesive layer that contacts the first surface of the substrate.

摘要翻译： 常开流体操纵阀组件和系统以及关闭，重新打开和重新关闭的方法。 常开阀组件可以包括包括第一表面的基板，其中第一和第二凹槽形成在第一表面中。 可以在第一表面中形成凹槽。 凹槽可以从第一凹槽延伸到第二凹槽，并且可以由具有第一弹性模量的第一可变形材料至少部分地限定。 阀组件还可以包括可弹性变形的盖。 可弹性变形的盖可以包括弹性变形材料层，其弹性模量大于第一可变形材料的弹性模量，以及接触基板的第一表面的粘合剂层。

公开(公告)号：US5384024A

公开(公告)日：1995-01-24

申请号：US850764

申请日：1992-03-13

申请人： Stephen E. Moring ,  Michael S. Albin ,  Reid B. Kowallis ,  Thomas E. Lee ,  Dennis E. Mead ,  John H. Nickel ,  Mark F. Oldham ,  Richard T. Reel ,  Timothy S. Orpin ,  Janice C. Woods

发明人： Stephen E. Moring ,  Michael S. Albin ,  Reid B. Kowallis ,  Thomas E. Lee ,  Dennis E. Mead ,  John H. Nickel ,  Mark F. Oldham ,  Richard T. Reel ,  Timothy S. Orpin ,  Janice C. Woods

IPC分类号： G01N27/447 ,  B01D61/46 ,  B01D57/02 ,  G01N27/26

CPC分类号： G01N27/44782

摘要： An instrument for capillary electrophoresis has a vertically translatable carrier holding one end of the capillary and an electrode connected to a power supply, and a rotary carousel for presenting containers of sample and buffer solutions to a load position where the end of the capillary and the electrode may be inserted by operating the carrier. The other end of the capillary and a second electrode connected to the power supply are immersed in an outlet buffer reservoir. The instrument has a vacuum supply for providing a relative vacuum over the solution in the outlet buffer reservoir, so simple material may be injected into the capillary either by electromigration or by differential pressure. After injection, the first end of the capillary and the electrode may be immersed in buffer to accomplish electrophoresis. By manipulating the carousel and carrier, multiple samples may be electrophoresed in series. In a preferred embodiment the instrument includes a flexible duck-billed septum for closing containers while permitting easy access for the electrode and the capillary tube. Also in a preferred embodiment the instrument is controlled by a microprocessor-based controller.

摘要翻译： 用于毛细管电泳的仪器具有保持毛细管的一端和连接到电源的电极的可垂直平移的载体，以及用于将样品和缓冲溶液的容器呈现到毛细管和电极的端部的负载位置的旋转转盘 可以通过操作载体来插入。 毛细管的另一端和连接到电源的第二电极浸入出口缓冲储存器中。 该仪器具有用于在出口缓冲储存器中的溶液上提供相对真空的真空源，因此可以通过电迁移或通过差压将简单的材料注入毛细管。 注射后，将毛细管和电极的第一端浸入缓冲液中以完成电泳。 通过操纵转盘和载体，可以将多个样品串联进行电泳。 在优选实施例中，该仪器包括用于封闭容器的柔性鸭嘴孔隔膜，同时允许容易地接近电极和毛细管。 同样在优选实施例中，仪器由基于微处理器的控制器控制。

公开(公告)号：US5597468A

公开(公告)日：1997-01-28

申请号：US324359

申请日：1994-10-17

申请人： Henk H. Lauer ,  Paul D. Grossman ,  Dennis E. Mead

发明人： Henk H. Lauer ,  Paul D. Grossman ,  Dennis E. Mead

IPC分类号： G01N27/447 ,  G01N27/26

CPC分类号： G01N27/44756 ,  G01N27/44743

摘要： An apparatus is disclosed for providing capillary electrophoresis, which includes an electronically controlled valve system for automatically introducing a sample into the capillary by means of a vacuum at the end of the capillary tube. This approach of sucking in the sample is extremely accurate and reproducible, and results in a minimum of band broadening. Furthermore, it enables the entire capillary electrophoresis sytem to be easily automated. An automated temperature control system is provided which enables the temperature of the capillary tube (and hence the solvent/solute system) to be controlled during electrophoresis, thereby very directly controlling pH and electrophoretic mobility. In another embodiment, the capillary is prewashed and equilibrated to achieve substantially zero charge on the capillary wall, thereby essentially eliminating electroosmotic flow and substantially improving resolution.

摘要翻译： 公开了一种用于提供毛细管电泳的装置，其包括电子控制阀系统，用于通过毛细管末端的真空将样品自动引入毛细管。 吸入样品的这种方法是非常准确和可重复的，并且导致最小的带宽变宽。 此外，它使得整个毛细管电泳系统能够容易地自动化。 提供了一种自动温度控制系统，其能够在电泳期间控制毛细管（因此溶剂/溶质系统）的温度，从而非常直接地控制pH和电泳迁移率。 在另一个实施方案中，毛细管被预洗和平衡以在毛细管壁上实现基本上零的电荷，从而基本上消除电渗流并显着提高分辨率。

公开(公告)号：US5240576A

公开(公告)日：1993-08-31

申请号：US461568

申请日：1990-01-05

申请人： Henk H. Lauer ,  Paul D. Grossman ,  Dennis E. Mead

发明人： Henk H. Lauer ,  Paul D. Grossman ,  Dennis E. Mead

IPC分类号： G01N27/447

CPC分类号： G01N27/44756 ,  G01N27/44743

摘要： an apparatus is disclosed for providing capillary electrophoresis, which includes an electronically controlled valve system for automatically introducing a sample into the capillary by means of a vacuum at the end of the capillary tube. This approach of sucking in the sample is extremely accurate and reproducible, and results in a minimum of band broadening. Furthermore, it enables the entire capillary electrophoresis sytem to be easily automated. An automated temperature control system is provided which enables the temperature of the capillary tube (and hence the solvent/solute system) to be controlled during electrophoresis, thereby very directly controlling pH and electrophoretic mobility. In another embodiment, the capillary is prewashed and equilibrated to achieve substantially zero charge on the capillary wall, thereby essentially eliminating electroosmotic flow and substantially improving resolution.

摘要翻译： 公开了一种用于提供毛细管电泳的装置，其包括用于通过毛细管末端的真空将样品自动引入毛细管的电子控制阀系统。 吸入样品的这种方法是非常准确和可重复的，并且导致最小的带宽变宽。 此外，它使得整个毛细管电泳系统能够容易地自动化。 提供了一种自动温度控制系统，其能够在电泳期间控制毛细管（因此溶剂/溶质系统）的温度，从而非常直接地控制pH和电泳迁移率。 在另一个实施方案中，毛细管被预洗和平衡以在毛细管壁上实现基本上零的电荷，从而基本上消除电渗流并显着提高分辨率。

公开(公告)号：US5239360A

公开(公告)日：1993-08-24

申请号：US561366

申请日：1990-07-31

申请人： Stephen E. Moring ,  Dennis E. Mead

发明人： Stephen E. Moring ,  Dennis E. Mead

IPC分类号： G01N21/25 ,  G01N27/447 ,  G01N30/74

CPC分类号： G01N27/44721 ,  G01N21/255 ,  G01N2201/0638 ,  G01N30/74

摘要： An apparatus for enhancing signal to noise ratio in the detection of electromagnetic radiation traversing a capillary tube includes a ball lens and a holding element for holding the ball lens and the capillary tube, the holding element having an aperture so that the aperature and the lens together define an optic axis. The holding element is configured to hold the capillary tube such that the center of the capillary tube traverses the optic axis. The lens has a focal length and is held at such a position relative to that focal length by the holding element such that electromagnetic radiation incident on the aperature is focussed to pass radially through the capillary tube. Thus, the effective path length is 100% of the inside diameter of the capillary tube.

摘要翻译： 在穿过毛细管的电磁辐射的检测中提高信噪比的装置包括球透镜和用于保持球透镜和毛细管的保持元件，保持元件具有孔，使得孔和透镜在一起 定义光轴。 保持元件被配置为保持毛细管，使得毛细管的中心横穿光轴。 透镜具有焦距并通过保持元件保持在相对于该焦距的这样的位置，使得入射在温度上的电磁辐射被聚焦以径向通过毛细管。 因此，有效路径长度为毛细管内径的100％。

公开(公告)号：US5207886A

公开(公告)日：1993-05-04

申请号：US767345

申请日：1991-09-27

申请人： Henk H. Lauer ,  Paul D. Grossman ,  Dennis E. Mead

发明人： Henk H. Lauer ,  Paul D. Grossman ,  Dennis E. Mead

IPC分类号： G01N27/447

CPC分类号： G01N27/44756 ,  G01N27/44743

摘要： An apparatus is disclosed for providing capillary electrophoresis, which includes an electronically controlled valve system for automatically introducing a sample into the capillary by means of a vacuum at the end of the capillary tube. This approach of sucking in the sample is extremely accurate and reproducible, and results in a minimum of band broadening. Furthermore, it enables the entire capillary electrophoresis system to be easily automated. An automated temperature control system is provided which enables the temperature of the capillary tube (and hence the solvent/solute system) to be controlled during electrophoresis, thereby very directly controlling pH and electrophoretic mobility. In another embodiment, the capillary is prewashed and equilibrated to achieve substantially zero charge on the capillary wall, thereby essentially eliminating electroosmotic flow and substantially improving resolution.

摘要翻译： 公开了一种用于提供毛细管电泳的装置，其包括电子控制阀系统，用于通过毛细管末端的真空将样品自动引入毛细管。 吸入样品的这种方法是非常准确和可重复的，并且导致最小的带宽变宽。 此外，它使得整个毛细管电泳系统能够容易地自动化。 提供了一种自动温度控制系统，其能够在电泳期间控制毛细管（因此溶剂/溶质系统）的温度，从而非常直接地控制pH和电泳迁移率。 在另一个实施方案中，毛细管被预洗和平衡以在毛细管壁上实现基本上零的电荷，从而基本上消除电渗流并显着提高分辨率。

公开(公告)号：US3995733A

公开(公告)日：1976-12-07

申请号：US313123

申请日：1972-12-07

申请人： Dennis E. Mead

发明人： Dennis E. Mead

IPC分类号： B65G27/04 ,  B65G27/00

CPC分类号： B65G27/04

摘要： A trough-shaped element is vibrated in a motion that includes a rise and fall of the trough, and the inside of the trough is lined with a pile material of flexible filaments uniformly inclined from the vertical toward the direction of feed along the trough. The filaments have a size, density and resilience so the tips of the filaments flexibly support and feed objects along the trough.

摘要翻译： 槽状元件在包括槽的上升和下降的运动中振动，并且槽的内部衬有柔性细丝的绒毛材料，其从垂直方向沿着槽沿着进给方向均匀地倾斜。 长丝具有尺寸，密度和弹性，使得长丝的尖端灵活地支撑并沿着槽输送物体。