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公开(公告)号：US09335532B2
公开(公告)日：2016-05-10
申请号：US11764064
申请日：2007-06-15
CPC分类号： G02B21/06 , G02B21/0032 , G02B21/0088 , G02B21/32
摘要： A laser assembly is provided which includes an objective assembly having an objective and a dichroic mirror, a collimating assembly having a collimating lens and a selectively displaceable mount, and a laser supply assembly having a laser source. The laser assembly can provide laser light through the collimating lens, which collimates the laser light into a collimated beam. The laser beam is preferably transmitted onto the dichroic mirror, which reflects the laser light through the objective along the optical path of the objective which converges into a focal point. Such a laser assembly preferably provides substantial control of the laser beam, for example, for micromanipulation of a sample.
摘要翻译： 提供了一种激光组件，其包括具有物镜和二向色镜的物镜组件，具有准直透镜和可选择性位移安装件的准直组件，以及具有激光源的激光供给组件。 激光组件可以通过准直透镜准直激光，该准直透镜将激光准直成准直光束。 激光束优选地被传送到二向色镜上，该分色镜反射激光通过物镜沿物镜的光路收敛成焦点。 这样的激光组件优选地提供激光束的实质控制，例如用于样品的显微操作。
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公开(公告)号：US06445451B1
公开(公告)日：2002-09-03
申请号：US09249943
申请日：1999-02-12
IPC分类号： H04J1500
CPC分类号： G01J3/50 , G01J3/0243 , G01J3/501
摘要： A cassette reader for detecting the status of stained samples is provided. The colorimeter includes one or more high power emitting diodes as the source of illuminating the sample. An elliptical mirror, with a central aperture to permit the light to penetrate on to the sample, directs the scattered light on to its focal point at a detector. The colorimeter includes a microprocessor which compares an output signal for the percent ratio value of the detected values of a sample and a standard sample. The colorimeter housing provides position sensors which allow the identification of the well being examined. Finally, the system is modified to measure cell motility by determining the ratio of motile sperms to total cell concentration.
摘要翻译： 提供了用于检测染色样品的状态的盒式读取器。 色度计包括一个或多个高功率发光二极管作为照明样品的源。 具有允许光穿透到样品的中心孔径的椭圆形反射镜将散射的光引导到检测器处的其焦点。 色度计包括微处理器，其比较了样品和标准样品的检测值的百分比值的输出信号。 色度计壳体提供位置传感器，其允许识别被检查的井。 最后，通过确定运动精子与总细胞浓度的比例来修改该系统以测量细胞运动性。
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公开(公告)号：US08422128B2
公开(公告)日：2013-04-16
申请号：US12615036
申请日：2009-11-09
IPC分类号： G02B21/06
CPC分类号： G02B21/082 , G02B21/02 , G02B27/141 , G02B27/34
摘要： A modular laser objective for use with a microscope is provided. A mounting modular body permits the modular objective to be releasably mounted to the turret of a microscope. The objective has an optical axis that permits an image beam to be emitted through the objective toward the eyepiece of a microscope. The modular body supports a mirror positioned at an angle to the optical axis of the objective. A modular laser assembly is mounted on the modular body on a first side of the mirror for directing a laser beam toward the mirror so that the energy is reflected off the mirror and through the objective in a direction that is substantially aligned with the optical axis of the objective. A modular indicator assembly is received in the modular body and includes a source of light positioned with the light incident on the other side of the mirror to reflect a beam of light in a direction opposite to the direction of the laser beam to provide an optical representation at the eyepiece of a microscope or on a camera of the position on the target of the laser beam being emitted by the objective.
摘要翻译： 提供了一种用于显微镜的模块化激光物镜。 安装的模块化主体允许模块化物镜可拆卸地安装在显微镜的转台上。 该目的具有允许图像束通过物镜朝向显微镜的目镜发射的光轴。 模块化主体支撑一个与物镜的光轴成角度定位的反射镜。 模块化激光组件安装在反射镜的第一侧上的模块体上，用于将激光束引向反射镜，使得能量被反射离开反射镜并通过物镜沿着基本上与光轴 的目标。 模块化指示器组件被接收在模块体中，并且包括定位成入射在反射镜的另一侧上的光的光源，以在与激光束的方向相反的方向上反射光束，以提供光学表示 在显微镜的目镜上或在目标发射的激光束的目标上的位置的照相机上。
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公开(公告)号：US20100118395A1
公开(公告)日：2010-05-13
申请号：US12615036
申请日：2009-11-09
IPC分类号： G02B21/06
CPC分类号： G02B21/082 , G02B21/02 , G02B27/141 , G02B27/34
摘要： A modular laser objective for use with a microscope is provided. A mounting modular body permits the modular objective to be releasably mounted to the turret of a microscope. The objective has an optical axis that penults an image beam to be emitted through the objective toward the eyepiece of a microscope. The modular body supports a mirror positioned at an angle to the optical axis of the objective. A modular laser assembly is mounted on the modular body on a first side of the mirror for directing a laser beam toward the mirror so that the energy is reflected off the mirror and through the objective in a direction that is substantially aligned with the optical axis of the objective. A modular indicator assembly is received in the modular body and includes a source of light positioned with the light incident on the other side of the mirror to reflect a beam of light in a direction opposite to the direction of the laser beam to provide an optical representation at the eyepiece of a microscope or on a camera of the position on the target of the laser beam being emitted by the objective.
摘要翻译： 提供了一种用于显微镜的模块化激光物镜。 安装的模块化主体允许模块化物镜可拆卸地安装在显微镜的转台上。 该物镜具有一个光轴，使得通过物镜朝向显微镜的目镜发射的图像光束。 模块化主体支撑一个与物镜的光轴成角度定位的反射镜。 模块化激光组件安装在反射镜的第一侧上的模块体上，用于将激光束引向反射镜，使得能量被反射离开反射镜并通过物镜沿着基本上与光轴 的目标。 模块化指示器组件被接收在模块体中，并且包括定位成入射在反射镜的另一侧上的光的光源，以在与激光束的方向相反的方向上反射光束，以提供光学表示 在显微镜的目镜上或在目标发射的激光束的目标上的位置的照相机上。
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公开(公告)号：US09040272B2
公开(公告)日：2015-05-26
申请号：US13158843
申请日：2011-06-13
摘要： The present invention provides a method and system for using eye-safe infrared energy from a Class I laser to manipulate cells in culture. The laser energy produces one or more phase boundary propulsion events, which generate hydrodynamic forces sufficient to manipulate cells at the focal point.
摘要翻译： 本发明提供一种使用来自I类激光的眼睛安全的红外能量来操纵培养细胞的方法和系统。 激光能量产生一个或多个相边界推进事件，其产生足以在焦点处操纵细胞的流体动力。
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公开(公告)号：US08149504B2
公开(公告)日：2012-04-03
申请号：US12481363
申请日：2009-06-09
IPC分类号： G02B21/06
CPC分类号： G02B21/04 , G02B21/0012
摘要： An objective assembly for use with a microscope is provided. The objective has an optical axis that permits an image beam to be emitted through the objective toward the eyepiece of a microscope. A mirror is positioned at an angle to the optical axis of the objective. A laser assembly is positioned on a first side of the mirror for directing a laser beam toward said mirror so that the energy is reflected off the mirror and through the objective in a direction that is substantially aligned with the optical axis of the objective. An indicator assembly including a source of light is positioned with the light incident on the other side of the mirror to reflect a beam of light in a direction opposite to the direction of the laser beam to provide an optical representation at the eyepiece of a microscope of the position of the laser beam being emitted by the objective.
摘要翻译： 提供了一种用于显微镜的目标组件。 该目的具有允许图像束通过物镜朝向显微镜的目镜发射的光轴。 反射镜与物镜的光轴成一角度定位。 激光器组件位于反射镜的第一侧上，用于将激光束引向所述反射镜，使得能量以与物镜的光轴基本对准的方向从镜子反射并穿过物镜。 包括光源的指示器组件定位成入射在反射镜的另一侧上的光以与激光束的方向相反的方向反射光束，以在显微镜的目镜处提供光学表示 激光束由物镜发射的位置。
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公开(公告)号：US20110306108A1
公开(公告)日：2011-12-15
申请号：US13158843
申请日：2011-06-13
摘要： The present invention provides a method and system for using eye-safe infrared energy from a Class I laser to manipulate cells in culture. The laser energy produces one or more phase boundary propulsion events, which generate hydrodynamic forces sufficient to manipulate cells at the focal point.
摘要翻译： 本发明提供一种使用来自I类激光的眼睛安全的红外能量来操纵培养细胞的方法和系统。 激光能量产生一个或多个相边界推进事件，其产生足以在焦点处操纵细胞的流体动力。
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公开(公告)号：US20090316259A1
公开(公告)日：2009-12-24
申请号：US12481363
申请日：2009-06-09
IPC分类号： G02B21/06
CPC分类号： G02B21/04 , G02B21/0012
摘要： An objective assembly for use with a microscope is provided. The objective has an optical axis that permits an image beam to be emitted through the objective toward the eyepiece of a microscope. A mirror is positioned at an angle to the optical axis of the objective. A laser assembly is positioned on a first side of the mirror for directing a laser beam toward said mirror so that the energy is reflected off the mirror and through the objective in a direction that is substantially aligned with the optical axis of the objective. An indicator assembly including a source of light is positioned with the light incident on the other side of the mirror to reflect a beam of light in a direction opposite to the direction of the laser beam to provide an optical representation at the eyepiece of a microscope of the position of the laser beam being emitted by the objective.
摘要翻译： 提供了一种用于显微镜的目标组件。 该目的具有允许图像束通过物镜朝向显微镜的目镜发射的光轴。 反射镜与物镜的光轴成一角度定位。 激光器组件位于反射镜的第一侧上，用于将激光束引向所述反射镜，使得能量以与物镜的光轴基本对准的方向从镜子反射并穿过物镜。 包括光源的指示器组件定位成入射在反射镜的另一侧上的光以与激光束的方向相反的方向反射光束，以在显微镜的目镜处提供光学表示 激光束由物镜发射的位置。
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9.发明授权Apparatus for measurement of cell concentration in a biological sample employing a magnetic slide loading apparatus 失效用于使用磁性载玻片加载装置测量生物样品中细胞浓度的装置
公开(公告)号：US5306467A
公开(公告)日：1994-04-26
申请号：US18734
申请日：1993-02-17
CPC分类号： B01L9/50 , G01N35/00029 , G01N2035/00039 , G01N2035/0477 , Y10S211/01 , Y10S359/903
摘要： A magnetic slide loading is used with automated cell counting equipment or manual optical microscopes to measure the number of cells in a fluid biological sample. A slide having a defined volume region holds the sample for measurement. A cover slip is placed over the defined volume region creating an enclosed volume. An actuable holder clamp is placed over the slide securing it as well as the cover slip in position by virtue of magnets secured to the bottom of the device exerting a magnetic force upon the holder clamp such that it is attracted toward the slide forcing the cover slip to flatten, thereby removing any defects or distortions from the volume. This action ensures that the volume has a uniform depth allowing accurate measurement of cell concentration within the biological sample.
摘要翻译： 使用磁性载玻片加载自动细胞计数设备或手动光学显微镜来测量流体生物样品中的细胞数量。 具有限定体积区域的载玻片保持用于测量的样品。 将盖子放置在限定的体积区域上，创建封闭的体积。 通过固定在装置的底部的磁体将固定它的滑盖以及盖子滑动件固定在滑块上，使可靠的夹具夹紧在支架夹具上施加磁力，使得它被朝着滑块吸引，迫使盖滑 变平，从而消除体积中的任何缺陷或变形。 该动作确保体积具有均匀的深度，可以准确测量生物样品中的细胞浓度。
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10.发明授权Method and device for cooling, preserving and safely transporting biological material 失效用于冷却，保存和安全运输生物材料的方法和装置
公开(公告)号：US4530816A
公开(公告)日：1985-07-23
申请号：US504578
申请日：1983-06-15
CPC分类号： B01L3/508 , F25D3/08 , B01L2300/1883 , F25D2303/081 , F25D2331/804 , F25D2600/04
摘要： A container for cooling, preserving and safely transporting a biological specimen includes a thermally insulating over-all container having as contents a container for ice, a container for the specimen including an isothermal metal cup, and a thermally insulating sheet interposed between the specimen container and the ice, the over-all container and the insulating sheet having thermal constants chosen to control the cooling rate, preferably to approximately one to three minutes per degree Centigrade, and to achieve a steady state temperature of the specimen near, but above freezing, preferably in the range of 4.degree. C. to 10.degree. C.The method of the invention includes the steps of packing biological specimens just after they are obtained into a specimen container and placing the specimen container near ice in an over-all insulating container, with a thermally insulating sheet between the ice and the specimen container, the thermal constants being chosen to cool down the specimen at an optimum cooling rate and to achieve an optimum steady state temperature for the specimen.
摘要翻译： 用于冷却，保存和安全运输生物样本的容器包括：具有作为内容物的容器的隔热整体容器，用于冰的容器，用于包括等温金属杯的试样的容器以及置于样品容器和 冰，整体容器和具有选择用于控制冷却速率的热常数的绝缘片，优选为每摄氏度约1至3分钟，并且为了使试样接近或高于冰点，优选地达到稳态 在4℃至10℃的范围内。本发明的方法包括以下步骤：将生物样品在获取到样品容器中之后进行包装，并将样品容器放置在冰附近的绝热容器中， 在冰和样品容器之间的隔热片，选择热常数以在o处冷却样品 最大冷却速率，并达到试样的最佳稳态温度。
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