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1.发明申请公开(公告)号：US20110003292A1
公开(公告)日：2011-01-06
申请号：US12747584
申请日：2008-12-11
申请人： Dimo Dietrich , Volker Liebenberg , Reimo Tetzner , Juergen Distler , Joern Lewin , Thomas Schlegel
发明人： Dimo Dietrich , Volker Liebenberg , Reimo Tetzner , Juergen Distler , Joern Lewin , Thomas Schlegel
CPC分类号： C12Q1/6886 , C12Q2600/154 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16
摘要： The invention provides methods, nucleic acids and kits for detecting lung carcinoma. The invention discloses genomic (FOXL2, ONECUT1, TFAP2E, EN2-2, EN2-3, SHOX2-2 and BARHL2) sequences the methylation patterns of which have utility for the improved detection of said disorder, thereby enabling the improved diagnosis and treatment of patients.
摘要翻译： 本发明提供用于检测肺癌的方法，核酸和试剂盒。 本发明公开了基因组（FOXL2，ONECUT1，TFAP2E，EN2-2，EN2-3，SHOX2-2和BARHL2）序列，其甲基化模式可用于改善所述病症的检测，从而能够改善患者的诊断和治疗 。
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2.发明申请公开(公告)号：US20090203011A1
公开(公告)日：2009-08-13
申请号：US12332703
申请日：2008-12-11
申请人： Volker Liebenberg , Juergen Distler , Joern Lewin , Fabian Model , Reimo Tetzner , Rene Cortese , Dimo Dietrich , Thomas Schlegel
发明人： Volker Liebenberg , Juergen Distler , Joern Lewin , Fabian Model , Reimo Tetzner , Rene Cortese , Dimo Dietrich , Thomas Schlegel
CPC分类号： C12Q1/6886 , C12Q2600/154 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16
摘要： Particular aspects provide methods, nucleic acids and kits for detecting cell proliferative disorders. Preferred aspects provide genomic sequences, the methylation patterns of which have substantial utility for the improved detection of said disorders, providing for improved diagnosis and treatment of same in patients.
摘要翻译： 具体方面提供用于检测细胞增殖性疾病的方法，核酸和试剂盒。 优选的方面提供基因组序列，其甲基化模式具有用于改善所述疾病的检测的实质性用途，从而提供对患者的相似性的改善的诊断和治疗。
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公开(公告)号：US20100092951A1
公开(公告)日：2010-04-15
申请号：US12310051
申请日：2007-08-01
申请人： Reimo Tetzner , Joern Lewin
发明人： Reimo Tetzner , Joern Lewin
CPC分类号： C12Q1/6858 , C12Q2537/113 , C12Q2535/119 , C12Q2523/125
摘要： The present invention relates to a method for methylation analysis. It comprises the providing of a double stranded nucleic acid; its conversion, whereby unmethylated bases become distinguishable in their base-pairing behavior from methylated bases, and the analysis of both of the converted nucleic acid strands.
摘要翻译： 本发明涉及甲基化分析方法。 它包括提供双链核酸; 其转化，其中未甲基化的碱基在其碱基配对行为与甲基化碱基之间变得可区分，以及两个转化的核酸链的分析。
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公开(公告)号：US08771939B2
公开(公告)日：2014-07-08
申请号：US12310051
申请日：2007-08-01
申请人： Reimo Tetzner , Joern Lewin
发明人： Reimo Tetzner , Joern Lewin
CPC分类号： C12Q1/6858 , C12Q2537/113 , C12Q2535/119 , C12Q2523/125
摘要： The present invention relates to a method for methylation analysis. It comprises the providing of a double stranded nucleic acid; its conversion, whereby unmethylated bases become distinguishable in their base-pairing behavior from methylated bases, and the analysis of both of the converted nucleic acid strands.
摘要翻译： 本发明涉及甲基化分析方法。 它包括提供双链核酸; 其转化，其中未甲基化的碱基在其碱基配对行为与甲基化碱基之间变得可区分，以及两个转化的核酸链的分析。
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公开(公告)号：US20100003680A1
公开(公告)日：2010-01-07
申请号：US12309555
申请日：2006-07-18
申请人： Joern Lewin , Ralf Lesche , Matthias Schuster , Dimo Dietrich
发明人： Joern Lewin , Ralf Lesche , Matthias Schuster , Dimo Dietrich
CPC分类号： C12Q1/6869
摘要： The invention relates to a method for quantitatively determining the methylation rate of a nucleic acid through sequencing. According to the invention, the method comprises at least the following steps: a) treating the nucleic acid with a chemical reagent or an enzyme containing solution, whereby the base pairing behavior of methylated cytosine bases and/or unmethylated cytosine bases of the nucleic acid are altered such that methylated cytosine bases become distinguishable from unmethylated cytosine bases, and b) introducing into the nucleic acid at least one base for generating a sequencing signal to be used as a reference signal for normalization, and c) sequencing the nucleic acid, whereby a signal from each cytosine base of the nucleic acid, or a signal from each guanine base of the nucleic acid and a reference signal from the at least on introduced base is obtained, and d) normalizing the signal obtained from each cytosine base of the nucleic acid, or the signal obtained from each guanine base of the nucleic acid to the reference signal from the at least one introduced base.
摘要翻译： 本发明涉及通过测序定量测定核酸的甲基化速率的方法。 根据本发明，该方法至少包括以下步骤：a）用化学试剂或含酶溶液处理核酸，由此核酸的甲基化胞嘧啶碱基和/或未甲基化的胞嘧啶碱基的碱基配对行为是 改变为使得甲基化胞嘧啶碱基与非甲基化胞嘧啶碱基区分开，以及b）将至少一个碱基引入核酸，以产生用作标准化参考信号的测序信号，和c）对核酸进行测序，由此 获得来自核酸的每个胞嘧啶碱基的信号或来自核酸的每个鸟嘌呤碱基的信号和来自至少一个引入的碱基的参考信号，以及d）使从核酸的每个胞嘧啶碱基获得的信号归一化 或从核酸的每个鸟嘌呤碱基获得的信号至至少一个引入的碱基的参考信号。
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公开(公告)号：US20080286778A1
公开(公告)日：2008-11-20
申请号：US11885706
申请日：2006-03-02
申请人： Joern Lewin , Juergen Distler , Ralf Lesche , Matthias Schuster
发明人： Joern Lewin , Juergen Distler , Ralf Lesche , Matthias Schuster
CPC分类号： C12Q1/683 , C12Q1/6848 , C12Q1/6858 , C12Q2523/125 , C12Q2521/331
摘要： The invention relates to a method for sensitively and specifically detecting cytosine methylations. For this purpose, DNA is first analysed by reacting with the aid of a methylation specific restriction enzyme. In such a way, the background DNA is removed from a reaction preparation. At a next step, a specific conversion of a non-methylated cytosine is carried out, while a methylated cytosine remains unchanged. The converted DNA can be analysed according to different methods, in particular by means of real time PCR method.
摘要翻译： 本发明涉及敏感地和特异性检测胞嘧啶甲基化的方法。 为此，首先通过与甲基化特异性限制性内切酶的反应进行DNA分析。 以这种方式，从反应制备物中除去背景DNA。 在下一步骤中，进行非甲基化胞嘧啶的特异转化，而甲基化胞嘧啶保持不变。 可以根据不同的方法，特别是通过实时PCR方法分析转化的DNA。
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7.发明申请公开(公告)号：US20110136115A1
公开(公告)日：2011-06-09
申请号：US12744318
申请日：2008-11-11
申请人： Andrew Z. Sledziewski , Shannon Payne , Matthias Schuster , Joern Lewin , Thomas Schlegel , Andrew M. Morotti
发明人： Andrew Z. Sledziewski , Shannon Payne , Matthias Schuster , Joern Lewin , Thomas Schlegel , Andrew M. Morotti
CPC分类号： C12Q1/6886 , C12Q2600/106 , C12Q2600/118 , C12Q2600/154 , C12Q2600/158
摘要： The invention provides methods, nucleic acids and kits for detecting prostate cell proliferative disorders. The invention discloses genomic sequences the methylation patterns of which have utility for the improved detection of said disorder, thereby enabling the improved diagnosis and treatment of patients.
摘要翻译： 本发明提供用于检测前列腺细胞增殖性疾病的方法，核酸和试剂盒。 本发明公开了基因组序列，其甲基化模式可用于改善所述病症的检测，从而能够改善患者的诊断和治疗。
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8.发明授权Method for the determination of the DNA methylation level of a CPG position in identical cells within a tissue sample 有权确定组织样品中相同细胞中CPG位置的DNA甲基化水平的方法公开(公告)号：US08912129B2
公开(公告)日：2014-12-16
申请号：US12085212
申请日：2006-11-17
申请人： Joern Lewin , Kurt Berlin
发明人： Joern Lewin , Kurt Berlin
CPC分类号： C12Q1/6827
摘要： Aspects of the present invention relate to the determination of the DNA methylation level at one or more CpG position within cells of a defined type in a tissue sample. This methylation level is deduced from the total DNA methylation level of all cells of the sample and from the content of said cells of interest. In aspects of the invention, the cell content is determined by means of histopatholoy, staining methods, antibodies, expression analysis or DNA methylation analysis.
摘要翻译： 本发明的方面涉及在组织样品中限定类型的细胞内的一个或多个CpG位置处的DNA甲基化水平的确定。 该甲基化水平是从样品所有细胞的总DNA甲基化水平以及所述感兴趣细胞的含量推断的。 在本发明的方面，通过组织病理学，染色方法，抗体，表达分析或DNA甲基化分析来确定细胞含量。
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9.发明申请METHODS AND COMPOSITIONS FOR DIFFERENTIATING TISSUES OR CELL TYPES USING EPIGENETIC MARKERS 审中-公开使用染色体标记分化组织或细胞类型的方法和组合物公开(公告)号：US20090170089A1
公开(公告)日：2009-07-02
申请号：US12036030
申请日：2008-02-22
申请人： Joern Lewin , Kurt Berlin , Thomas Hildmann , Alexander Olek , Stephan Beck , Karen Novik
发明人： Joern Lewin , Kurt Berlin , Thomas Hildmann , Alexander Olek , Stephan Beck , Karen Novik
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6881 , C12Q2600/154 , C12Q2600/16
摘要： The present invention provides, inter alia, a method for generating a genome-wide epigenomic map, comprising a correlation between methylation variable CpG positions (MVP) and genomic DNA sample types. MVP are those CpG positions that show a variable quantitative level of methylation between sample types. Particular genomic regions of interest (ROI) provide preferred marker sequences that comprise multiple, and preferably proximate MVP, and that have novel utility for distinguishing sample types. The epigenic maps have broad utility, for example, in identifying sample types, or for distinguishing between and among sample types. In a preferred embodiment the epigenomic map is based on methylation variable regions (MVP) within the major histocompatibility complex (MHC), and has utility, for example, in identifying the cell or tissue source of a genomic DNA sample, or for distinguishing one or more particular cell or tissue types among other cell or tissue types. Analysis of epigenetic characteristics of one, or of a set of nucleic acid sequences, in the context of an inventive epigenomic map, allows for the determination of an origin of the nucleic acids.
摘要翻译： 本发明尤其提供了一种生成全基因组表观遗传学图谱的方法，其包括甲基化可变CpG位点（MVP）和基因组DNA样品类型之间的相关性。 MVP是显示样品类型之间甲基化可变定量水平的那些CpG位置。 特定的感兴趣的基因组区域（ROI）提供优选的标记序列，其包含多个，优选接近MVP，并且具有用于区分样品类型的新颖效用。 表征性地图具有广泛的用途，例如，在识别样本类型中，或用于区分样本类型和样本类型。 在优选的实施方案中，表观基因组图基于主要组织相容性复合物（MHC）内的甲基化可变区（MVP），并且可用于例如鉴定基因组DNA样品的细胞或组织来源，或用于区分一个或 更特定的细胞或组织类型与其他细胞或组织类型。 在本发明表观遗传学图谱的上下文中，一种或一组核酸序列的表观遗传学特征的分析允许确定核酸的来源。
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公开(公告)号：US20110027789A1
公开(公告)日：2011-02-03
申请号：US12848239
申请日：2010-08-02
申请人： Joern Lewin
发明人： Joern Lewin
CPC分类号： C12Q1/6806 , C12Q1/6858 , C12Q1/6886 , C12Q2600/154 , C12Q2523/125 , C12Q2521/125
摘要： The present invention relates to methods and kits for preserving genomic DNA sequence complexity within chemically and/or enzymatically converted DNA by an enzyme or series of enzymes that adds a methyl group to a cytosine outside of CpG dinucleotide sequences of genomic DNA. Further, the present invention relates to methylation analysis of the genomic DNA.
摘要翻译： 本发明涉及通过酶或一系列酶在基因组DNA的CpG二核苷酸序列之外的胞嘧啶中添加甲基而在化学和/或酶转化的DNA中保存基因组DNA序列复杂性的方法和试剂盒。 此外，本发明涉及基因组DNA的甲基化分析。
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