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223 条记录 (耗时 0.041 秒)

    Method for generating aptamers with improved off-rates
    1.
    发明授权
    Method for generating aptamers with improved off-rates 有权
    用于产生具有改善的脱离率的适配体的方法

    公开(公告)号：US08975026B2

    公开(公告)日：2015-03-10

    申请号：US13382493

    申请日：2010-07-09

    申请人： Dominic Zichi ,  Sheri K. Wilcox ,  Chris Bock ,  Daniel J. Schneider ,  Bruce Eaton ,  Larry Gold ,  Thale C. Jarvis ,  Jeffrey D. Carter

    发明人： Dominic Zichi ,  Sheri K. Wilcox ,  Chris Bock ,  Daniel J. Schneider ,  Bruce Eaton ,  Larry Gold ,  Thale C. Jarvis ,  Jeffrey D. Carter

    IPC分类号： C12N15/115

    CPC分类号： C12N15/1048 ,  C12N15/115 ,  C12N2310/16 ,  C12Q1/6811 ,  G01N33/5308 ,  C12Q2525/117 ,  C12Q2525/205 ,  C12Q2541/101

    摘要： The present disclosure describes the identification and use of aptamers and photoaptamers having slower dissociation rate constants than those obtained using previously described methods. Specifically, the present disclosure describes methods for the identification and use of aptamers to one or more targets within a histological or cytological sample, which have slow rates of dissociation. The aptamers may be used to assess localization, relative density, and presence or absence of one or more targets in cytological and histological samples. Targets may be selected that are specific and diagnostic of a given disease state for which the sample was collected. The aptamers may also be used to introduce target specific signal moieties. In addition to target identification, the aptamers may be used to amplify signal generation through a variety of methods.

    摘要翻译： 本公开描述了具有比使用先前描述的方法获得的那些更快的解离速率常数的适配体和光致抗体的鉴定和使用。 具体地，本公开描述了用于鉴定和使用适配体到组织学或细胞学样品中的一种或多种目标的方法，其具有缓慢的解离速率。 适配体可用于评估细胞学和组织学样品中的一种或多种靶标的定位，相对密度和存在或不存在。 可以选择对于样品收集的给定疾病状态的特异性和诊断性的靶标。 适体也可以用于引入目标特异性信号部分。 除目标识别之外，适体可用于通过各种方法放大信号产生。

    Methods and reagents for detecting target binding by nucleic acid ligands
    4.
    发明申请
    Methods and reagents for detecting target binding by nucleic acid ligands 审中-公开
    用于检测核酸配体靶结合的方法和试剂

    公开(公告)号：US20060057573A1

    公开(公告)日：2006-03-16

    申请号：US10504696

    申请日：2003-02-10

    申请人： Larry Gold ,  Jonathan Smith ,  Dominic Zichi ,  Daniel Schneider ,  Chad Greef

    发明人： Larry Gold ,  Jonathan Smith ,  Dominic Zichi ,  Daniel Schneider ,  Chad Greef

    IPC分类号： C12Q1/68 ,  C12Q1/42

    CPC分类号： C12N15/115 ,  G01N33/58

    摘要： The present invention provides novel methods and reagents for detecting the binding of protein targets to nucleic acid ligands. Using Universal Protein Stains (UPS), proteins bound by nucleic acid ligands may be labeled with a detectable moiety. The methods and reagents are particularly useful for the detection of protein targets bound to multiplexed arrays of nucleic acid ligands. The present invention also provides novel methods for the multiplexed evaluation of photocrosslinking nucleic acid ligands. The methods allow one simultaneously to: (1) evaluate the performance (dynamic range) of a plurality of photocrosslinking nucleic acid ligands; and (2) assess the specificity of each photocrosslinking nucleic acid ligand for its cognate target protein. Photocrosslinking nucleic acid ligands with the most desirable properties can then be selected for use in diagnostic and prognostic medical assays. The present invention also provides a photocrosslinking nucleic acid ligand that binds specifically to HIV gp120MN.

    摘要翻译： 本发明提供用于检测蛋白质靶标与核酸配体的结合的新型方法和试剂。 使用通用蛋白污渍（UPS），可以用可检测的部分标记由核酸配体结合的蛋白质。 所述方法和试剂对于检测与核酸配体复合阵列结合的蛋白质靶标特别有用。 本发明还提供了用于光交联核酸配体的多重评价的新方法。 该方法同时允许：（1）评估多个光交联核酸配体的性能（动态范围）; 和（2）评估每个光交联核酸配体对其同源靶蛋白的特异性。 然后可以选择具有最理想特性的光交联核酸配体用于诊断和预后医学测定。 本发明还提供了与HIV gp120MN特异性结合的光交联核酸配体。

    Selex and photoSELEX
    5.
    发明授权
    Selex and photoSELEX 有权
    Selex和photoSELEX

    公开(公告)号：US08409795B2

    公开(公告)日：2013-04-02

    申请号：US12175388

    申请日：2008-07-17

    申请人： Daniel J. Schneider ,  Sheri K. Wilcox ,  Dominic Zichi ,  Dan Nieuwlandt ,  Jeff Carter ,  Larry Gold

    发明人： Daniel J. Schneider ,  Sheri K. Wilcox ,  Dominic Zichi ,  Dan Nieuwlandt ,  Jeff Carter ,  Larry Gold

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6811 ,  C12N15/1048 ,  C12N15/111 ,  C12N15/115 ,  C12N2310/16 ,  C12N2320/13 ,  C12Q2541/101 ,  C12Q2525/205 ,  C12Q2525/117

    摘要： The present disclosure describes improved SELEX methods for generating nucleic acid ligands that are capable of binding to target molecules and improved photoSELEX methods for generating photoreactive nucleic acid ligands that are capable of both binding and covalently crosslinking to target molecules. The disclosure further describes nucleic acid libraries having expanded physical and chemical properties and their use in SELEX and photoSELEX; methods for increasing the crosslinking efficiencies of photoaptamers; methods for producing photoaptamers having selective modifications that enhance functionality and minimize non-specific photoreactions; and methods for generating truncated nucleic acid ligands from nucleic acid ligands of longer length. The disclosure further describes aptamers and photoaptamers obtained by using any of the foregoing.

    摘要翻译： 本公开描述了用于产生能够结合靶分子的核酸配体的改进的SELEX方法和用于产生能够与靶分子结合和共价交联的光反应性核酸配体的改进的photoSELEX方法。 本公开进一步描述了具有扩展的物理和化学性质及其在SELEX和photoSELEX中的用途的核酸文库; 提高光催化剂交联效率的方法; 制备具有增强功能并使非特异性光反应最小化的选择性修饰的光致抗体的方法; 以及用于从较长长度的核酸配体产生截短的核酸配体的方法。 本公开进一步描述了通过使用上述任何一种获得的适体和光致抗体。

    Method for generating aptamers with improved off-rates
    7.
    发明授权
    Method for generating aptamers with improved off-rates 有权
    用于产生具有改善的脱离率的适配体的方法

    公开(公告)号：US07964356B2

    公开(公告)日：2011-06-21

    申请号：US12499967

    申请日：2009-07-09

    申请人： Dominic Zichi ,  Sheri K. Wilcox ,  Chris Bock ,  Daniel J. Schneider ,  Bruce Eaton ,  Larry Gold

    发明人： Dominic Zichi ,  Sheri K. Wilcox ,  Chris Bock ,  Daniel J. Schneider ,  Bruce Eaton ,  Larry Gold

    IPC分类号： C12Q1/68 ,  C07H21/02

    CPC分类号： C12N15/1048 ,  C12Q1/6811 ,  C12Q2525/205

    摘要： The present disclosure describes methods for producing aptamers and photoaptamers having slower dissociation rate constants than are obtained using prior SELEX and photoSELEX methods. The disclosure further describes aptamers and photoaptamers having slower dissociation rate constants than those obtained using prior methods. This invention relates to the field of diagnostic histology, cytology, histopathology, and cytopathology methods and reagents for the detection of various disease states. More specifically, the invention relates to the use of aptamers in histologic, cytologic, histopathic, and/or cytopathic diagnostic methods. Aptamers may be provided that react with specific target molecules contained within a histological or cytological sample. Aptamers may be used to assess localization, relative density, and presence or absence of one or more target. Targets may be selected that are specific and diagnostic of a given disease state for which the sample was collected. Aptamers may be used to introduce target specific signal moieties. Antigen retrieval methods may be applied to the sample prior to reaction with the specific aptamer/s to improve interaction of the aptamer and target within the sample. Or aptamers may be developed for the specific target that eliminates the need for the antigen retrieval process. In addition to target identification, aptamers may be used to amplify signal generation through a variety of methods.

    摘要翻译： 本公开描述了用于产生具有比使用先前的SELEX和photoSELEX方法获得的解离速率常数更慢的适体和光催化剂的方法。 本公开进一步描述了具有比使用现有方法获得的那些更快的解离速率常数的适体和光致抗体。 本发明涉及用于检测各种疾病状态的诊断组织学，细胞学，组织病理学和细胞病理学方法和试剂领域。 更具体地，本发明涉及适配体在组织学，细胞学，组织病理学和/或细胞病变诊断方法中的用途。 可以提供与包含在组织学或细胞学样品中的特定靶分子反应的适配子。 可以使用适配器来评估定位，相对密度以及一个或多个靶的存在或不存在。 可以选择对于样品收集的给定疾病状态的特异性和诊断性的靶标。 适配子可用于引入目标特异性信号部分。 抗原检索方法可以在与特异性适体反应之前应用于样品，以改善样品中适体和靶标的相互作用。 或者可以针对特定靶标开发适配体，从而消除对抗原检索过程的需要。 除目标识别之外，适体可用于通过各种方法放大信号产生。

    NUCLEIC ACID LIGAND DIAGNOSTIC BIOCHIP
    9.
    发明申请
    NUCLEIC ACID LIGAND DIAGNOSTIC BIOCHIP 审中-公开
    核酸配对生物鉴定

    公开(公告)号：US20070166742A1

    公开(公告)日：2007-07-19

    申请号：US11623822

    申请日：2007-01-17

    申请人： Larry Gold ,  Dominic Zichi

    发明人： Larry Gold ,  Dominic Zichi

    IPC分类号： C12Q1/68 ,  C12M3/00

    CPC分类号： C12Q1/6839 ,  C07B2200/11 ,  C12Q1/6834 ,  C12Q1/6837 ,  C40B40/00 ,  Y10S977/924 ,  C12Q2565/514 ,  C12Q2565/101 ,  C12Q2541/101 ,  C12Q2525/205

    摘要： A nucleic acid ligand “biochip” is disclosed, consisting of a solid support to which one or more specific nucleic acid ligands is attached in a spatially defined manner. Each nucleic acid ligand binds specifically and avidly to a particular target molecule contained within a test mixture, such as a bodily fluid. The target molecules include, but are not limited to, proteins (cellular, viral, bacterial, etc.) hormones, sugars, metabolic byproducts, cofactor, and intermediates, drugs, and toxins. Contacting the test mixture with the biochip leads to the binding of a target molecule to its cognate nucleic acid ligand. The biochip may then be contacted with a reagent(s) that reacts covalently with proteins and not with nucleic acids. Each protein target in the test mixture may then detected by detecting the presence of the reagent at the appropriate address on the biochip.

    摘要翻译： 公开了一种核酸配体“生物芯片”，其由以空间限定的方式连接一个或多个特定核酸配体的固体支持物组成。 每个核酸配体特异性地并且强烈地结合到测试混合物（例如体液）中所含的特定靶分子。 靶分子包括但不限于蛋白质（细胞，病毒，细菌等）激素，糖，代谢副产物，辅因子和中间体，药物和毒素。 将测试混合物与生物芯片接触导致靶分子与其同源核酸配体的结合。 然后生物芯片可以与与蛋白质共价反应的试剂与不与核酸反应的试剂接触。 然后可以通过检测生物芯片上适当地址处的试剂的存在来检测测试混合物中的每个蛋白质靶标。

    MULTIPLEXED ANALYSES OF TEST SAMPLES
    10.
    发明申请
    MULTIPLEXED ANALYSES OF TEST SAMPLES 审中-公开
    测试样本的多重分析

    公开(公告)号：US20070166740A1

    公开(公告)日：2007-07-19

    申请号：US11623535

    申请日：2007-01-16

    申请人： James Heil ,  Daniel Schneider ,  Daniel Nieuwlandt ,  Sheri Wilcox ,  Dominic Zichi ,  Todd Gander ,  Bruce Eaton ,  Larry Gold

    发明人： James Heil ,  Daniel Schneider ,  Daniel Nieuwlandt ,  Sheri Wilcox ,  Dominic Zichi ,  Todd Gander ,  Bruce Eaton ,  Larry Gold

    IPC分类号： C12Q1/68 ,  G01N33/53

    CPC分类号： G01N33/5308 ,  C12N15/115 ,  C12N2310/16 ,  C12N2310/3519 ,  C12Q1/6816 ,  C12Q1/6832 ,  C12Q1/6834 ,  G01N33/58 ,  C12Q2565/514 ,  C12Q2525/205 ,  C12Q2525/161 ,  C12Q2537/101 ,  C12Q2523/313

    摘要： The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. In one embodiment, a test sample is contacted with an aptamer that includes a tag and has a specific affinity for a target molecule. An aptamer affinity complex that includes an aptamer bound to its target molecule is allowed to form. If the test sample contains the target molecule, an aptamer affinity complex will generally form in the test sample. The aptamer affinity complex is optionally converted to an aptamer covalent complex that includes an aptamer covalently bound to its target molecule. The aptamer affinity complex (or optional aptamer covalent complex) can then be detected and/or quantified using any of a variety of methods known to one skilled in the art, including using a solid support, using mass spectrometry, and using quantitative polymerase chain reaction (Q-PCR).

    摘要翻译： 本公开描述了用于检测可能存在于测试样品中的一种或多种靶分子的方法，装置，试剂和试剂盒。 在一个实施方案中，将测试样品与包含标签的适体接触并对靶分子具有特异性亲和力。 允许形成包含与其靶分子结合的适体的适体亲和复合体。 如果测试样品含有目标分子，通常将在测试样品中形成适体亲和复合体。 适体亲和复合物任选地转化为适体共价复合物，其包括与其靶分子共价结合的适体。 然后可以使用本领域技术人员已知的各种方法中的任一种检测和/或定量适体亲和复合物（或任选的适体共价复合物），包括使用固体支持物，使用质谱法和使用定量聚合酶链反应 （Q-PCR）。