-
1.发明申请公开(公告)号：US20060057573A1
公开(公告)日：2006-03-16
申请号：US10504696
申请日：2003-02-10
申请人： Larry Gold , Jonathan Smith , Dominic Zichi , Daniel Schneider , Chad Greef
发明人： Larry Gold , Jonathan Smith , Dominic Zichi , Daniel Schneider , Chad Greef
CPC分类号： C12N15/115 , G01N33/58
摘要： The present invention provides novel methods and reagents for detecting the binding of protein targets to nucleic acid ligands. Using Universal Protein Stains (UPS), proteins bound by nucleic acid ligands may be labeled with a detectable moiety. The methods and reagents are particularly useful for the detection of protein targets bound to multiplexed arrays of nucleic acid ligands. The present invention also provides novel methods for the multiplexed evaluation of photocrosslinking nucleic acid ligands. The methods allow one simultaneously to: (1) evaluate the performance (dynamic range) of a plurality of photocrosslinking nucleic acid ligands; and (2) assess the specificity of each photocrosslinking nucleic acid ligand for its cognate target protein. Photocrosslinking nucleic acid ligands with the most desirable properties can then be selected for use in diagnostic and prognostic medical assays. The present invention also provides a photocrosslinking nucleic acid ligand that binds specifically to HIV gp120MN.
摘要翻译： 本发明提供用于检测蛋白质靶标与核酸配体的结合的新型方法和试剂。 使用通用蛋白污渍（UPS），可以用可检测的部分标记由核酸配体结合的蛋白质。 所述方法和试剂对于检测与核酸配体复合阵列结合的蛋白质靶标特别有用。 本发明还提供了用于光交联核酸配体的多重评价的新方法。 该方法同时允许：（1）评估多个光交联核酸配体的性能（动态范围）; 和（2）评估每个光交联核酸配体对其同源靶蛋白的特异性。 然后可以选择具有最理想特性的光交联核酸配体用于诊断和预后医学测定。 本发明还提供了与HIV gp120MN特异性结合的光交联核酸配体。
-
2.发明授权公开(公告)号：US08071288B2
公开(公告)日：2011-12-06
申请号：US12030024
申请日：2008-02-12
CPC分类号： C12N15/115 , G01N33/58
摘要： The present invention provides novel methods and reagents for detecting the binding of protein targets to nucleic acid ligands. Using Universal Protein Stains (UPS), proteins bound by nucleic acid ligands may be labeled with a detectable moiety. The methods and reagents are particularly useful for the detection of protein targets bound to multiplexed arrays of nucleic acid ligands. The present invention also provides novel methods for the multiplexed evaluation of photocrosslinking nucleic acid ligands. The methods allow one simultaneously to: (1) evaluate the performance (dynamic range) of a plurality of photocrosslinking nucleic acid ligands; and (2) assess the specificity of each photocrosslinking nucleic acid ligand for its cognate target protein. Photocrosslinking nucleic acid ligands with the most desirable properties can then be selected for use in diagnostic and prognostic medical assays. The present invention also provides a photocrosslinking nucleic acid ligand that binds specifically to HIV gp120MN.
摘要翻译： 本发明提供用于检测蛋白质靶标与核酸配体的结合的新型方法和试剂。 使用通用蛋白污渍（UPS），可以用可检测的部分标记由核酸配体结合的蛋白质。 所述方法和试剂对于检测与核酸配体复合阵列结合的蛋白质靶标特别有用。 本发明还提供了用于光交联核酸配体的多重评价的新方法。 该方法同时允许：（1）评估多个光交联核酸配体的性能（动态范围）; 和（2）评估每个光交联核酸配体对其同源靶蛋白的特异性。 然后可以选择具有最理想特性的光交联核酸配体用于诊断和预后医学测定。 本发明还提供了与HIV gp120MN特异性结合的光交联核酸配体。
-
3.发明申请公开(公告)号：US20080160535A1
公开(公告)日：2008-07-03
申请号：US12030024
申请日：2008-02-12
CPC分类号： C12N15/115 , G01N33/58
摘要： The present invention provides novel methods and reagents for detecting the binding of protein targets to nucleic acid ligands. Using Universal Protein Stains (UPS), proteins bound by nucleic acid ligands may be labeled with a detectable moiety. The methods and reagents are particularly useful for the detection of protein targets bound to multiplexed arrays of nucleic acid ligands. The present invention also provides novel methods for the multiplexed evaluation of photocrosslinking nucleic acid ligands. The methods allow one simultaneously to: (1) evaluate the performance (dynamic range) of a plurality of photocrosslinking nucleic acid ligands; and (2) assess the specificity of each photocrosslinking nucleic acid ligand for its cognate target protein. Photocrosslinking nucleic acid ligands with the most desirable properties can then be selected for use in diagnostic and prognostic medical assays. The present invention also provides a photocrosslinking nucleic acid ligand that binds specifically to HIV gp120MN.
摘要翻译： 本发明提供用于检测蛋白质靶标与核酸配体的结合的新型方法和试剂。 使用通用蛋白污渍（UPS），可以用可检测的部分标记由核酸配体结合的蛋白质。 所述方法和试剂对于检测与核酸配体复合阵列结合的蛋白质靶标特别有用。 本发明还提供了用于光交联核酸配体的多重评价的新方法。 该方法同时允许：（1）评估多个光交联核酸配体的性能（动态范围）; 和（2）评估每个光交联核酸配体对其同源靶蛋白的特异性。 然后可以选择具有最理想特性的光交联核酸配体用于诊断和预后医学测定。 本发明还提供了与HIV gp120MN特异性结合的光交联核酸配体。
-
公开(公告)号：US20070166740A1
公开(公告)日：2007-07-19
申请号：US11623535
申请日：2007-01-16
申请人： James Heil , Daniel Schneider , Daniel Nieuwlandt , Sheri Wilcox , Dominic Zichi , Todd Gander , Bruce Eaton , Larry Gold
发明人： James Heil , Daniel Schneider , Daniel Nieuwlandt , Sheri Wilcox , Dominic Zichi , Todd Gander , Bruce Eaton , Larry Gold
CPC分类号： G01N33/5308 , C12N15/115 , C12N2310/16 , C12N2310/3519 , C12Q1/6816 , C12Q1/6832 , C12Q1/6834 , G01N33/58 , C12Q2565/514 , C12Q2525/205 , C12Q2525/161 , C12Q2537/101 , C12Q2523/313
摘要： The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. In one embodiment, a test sample is contacted with an aptamer that includes a tag and has a specific affinity for a target molecule. An aptamer affinity complex that includes an aptamer bound to its target molecule is allowed to form. If the test sample contains the target molecule, an aptamer affinity complex will generally form in the test sample. The aptamer affinity complex is optionally converted to an aptamer covalent complex that includes an aptamer covalently bound to its target molecule. The aptamer affinity complex (or optional aptamer covalent complex) can then be detected and/or quantified using any of a variety of methods known to one skilled in the art, including using a solid support, using mass spectrometry, and using quantitative polymerase chain reaction (Q-PCR).
摘要翻译： 本公开描述了用于检测可能存在于测试样品中的一种或多种靶分子的方法，装置，试剂和试剂盒。 在一个实施方案中，将测试样品与包含标签的适体接触并对靶分子具有特异性亲和力。 允许形成包含与其靶分子结合的适体的适体亲和复合体。 如果测试样品含有目标分子，通常将在测试样品中形成适体亲和复合体。 适体亲和复合物任选地转化为适体共价复合物，其包括与其靶分子共价结合的适体。 然后可以使用本领域技术人员已知的各种方法中的任一种检测和/或定量适体亲和复合物（或任选的适体共价复合物），包括使用固体支持物，使用质谱法和使用定量聚合酶链反应 （Q-PCR）。
-
公开(公告)号：US20070166741A1
公开(公告)日：2007-07-19
申请号：US11623580
申请日：2007-01-16
申请人： James Heil , Daniel Schneider , Daniel Nieuwlandt , Sheri Wilcox , Dominic Zichi , Todd Gander , Bruce Eaton , Larry Gold
发明人： James Heil , Daniel Schneider , Daniel Nieuwlandt , Sheri Wilcox , Dominic Zichi , Todd Gander , Bruce Eaton , Larry Gold
摘要： The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. In one embodiment, a test sample is contacted with an aptamer that includes a tag and has a specific affinity for a target molecule. An aptamer affinity complex that includes an aptamer bound to its target molecule is allowed to form. If the test sample contains the target molecule, an aptamer affinity complex will generally form in the test sample. The aptamer affinity complex is optionally converted to an aptamer covalent complex that includes an aptamer covalently bound to its target molecule. The aptamer affinity complex (or optional aptamer covalent complex) can then be detected and/or quantified using any of a variety of methods known to one skilled in the art, including using a solid support, using mass spectrometry, and using quantitative polymerase chain reaction (Q-PCR).
-
公开(公告)号：US08975026B2
公开(公告)日：2015-03-10
申请号：US13382493
申请日：2010-07-09
申请人： Dominic Zichi , Sheri K. Wilcox , Chris Bock , Daniel J. Schneider , Bruce Eaton , Larry Gold , Thale C. Jarvis , Jeffrey D. Carter
发明人： Dominic Zichi , Sheri K. Wilcox , Chris Bock , Daniel J. Schneider , Bruce Eaton , Larry Gold , Thale C. Jarvis , Jeffrey D. Carter
IPC分类号： C12N15/115
CPC分类号： C12N15/1048 , C12N15/115 , C12N2310/16 , C12Q1/6811 , G01N33/5308 , C12Q2525/117 , C12Q2525/205 , C12Q2541/101
摘要： The present disclosure describes the identification and use of aptamers and photoaptamers having slower dissociation rate constants than those obtained using previously described methods. Specifically, the present disclosure describes methods for the identification and use of aptamers to one or more targets within a histological or cytological sample, which have slow rates of dissociation. The aptamers may be used to assess localization, relative density, and presence or absence of one or more targets in cytological and histological samples. Targets may be selected that are specific and diagnostic of a given disease state for which the sample was collected. The aptamers may also be used to introduce target specific signal moieties. In addition to target identification, the aptamers may be used to amplify signal generation through a variety of methods.
摘要翻译： 本公开描述了具有比使用先前描述的方法获得的那些更快的解离速率常数的适配体和光致抗体的鉴定和使用。 具体地，本公开描述了用于鉴定和使用适配体到组织学或细胞学样品中的一种或多种目标的方法，其具有缓慢的解离速率。 适配体可用于评估细胞学和组织学样品中的一种或多种靶标的定位，相对密度和存在或不存在。 可以选择对于样品收集的给定疾病状态的特异性和诊断性的靶标。 适体也可以用于引入目标特异性信号部分。 除目标识别之外，适体可用于通过各种方法放大信号产生。
-
公开(公告)号：US08945830B2
公开(公告)日：2015-02-03
申请号：US12859930
申请日：2010-08-20
申请人： James R. Heil , Daniel J. Schneider , Daniel T. Nieuwlandt , Sheri K. Wilcox , Dominic Zichi , Todd Gander , Bruce Eaton , Larry Gold
发明人： James R. Heil , Daniel J. Schneider , Daniel T. Nieuwlandt , Sheri K. Wilcox , Dominic Zichi , Todd Gander , Bruce Eaton , Larry Gold
CPC分类号： G01N33/54306 , C07B2200/11 , C12Q1/6832 , C12Q1/6834 , C12Q1/6837 , C40B40/00 , G01N33/58 , C12Q2537/101 , C12Q2525/205 , C12Q2523/313 , C12Q2525/161 , C12Q2565/514 , C12Q2565/101 , C12Q2541/101
摘要： The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. In one embodiment, a test sample is contacted with an aptamer that includes a tag and has a specific affinity for a target molecule. An aptamer affinity complex that includes an aptamer bound to its target molecule is allowed to form. If the test sample contains the target molecule, an aptamer affinity complex will generally form in the test sample. The aptamer affinity complex is optionally converted to an aptamer covalent complex that includes an aptamer covalently bound to its target molecule. The aptamer affinity complex (or optional aptamer covalent complex) can then be detected and/or quantified using any of a variety of methods known to one skilled in the art, including using a solid support, using mass spectrometry, and using quantitative polymerase chain reaction (Q-PCR).
摘要翻译： 本公开描述了用于检测可能存在于测试样品中的一种或多种靶分子的方法，装置，试剂和试剂盒。 在一个实施方案中，将测试样品与包含标签的适体接触并对靶分子具有特异性亲和力。 允许形成包含与其靶分子结合的适体的适体亲和复合体。 如果测试样品含有目标分子，通常将在测试样品中形成适体亲和复合体。 适体亲和复合物任选地转化为适体共价复合物，其包括与其靶分子共价结合的适体。 然后可以使用本领域技术人员已知的各种方法中的任一种检测和/或定量适体亲和复合物（或任选的适体共价复合物），包括使用固体支持物，使用质谱法和使用定量聚合酶链反应 （Q-PCR）。
-
公开(公告)号：US20100086948A1
公开(公告)日：2010-04-08
申请号：US12574341
申请日：2009-10-06
申请人： Larry Gold , Marty Stanton , Edward N. Brody , Rachel M. Ostroff , Dominic Zichi , Alex A.E. Stewart
发明人： Larry Gold , Marty Stanton , Edward N. Brody , Rachel M. Ostroff , Dominic Zichi , Alex A.E. Stewart
IPC分类号： G01N33/567 , G01N33/53 , G06F19/00 , G06F17/18
CPC分类号： G01N33/57449 , G16B40/00 , Y10T436/143333
摘要： The present application includes biomarkers, methods, devices, reagents, systems, and kits for the detection and diagnosis of ovarian cancer. In one aspect, the application provides biomarkers that can be used alone or in various combinations to diagnose ovarian cancer or permit the differential diagnosis of a pelvic mass as benign or malignant. In another aspect, methods are provided for diagnosing ovarian cancer in an individual, where the methods include detecting, in a biological sample from an individual, at least one biomarker value corresponding to at least one biomarker selected from the group of biomarkers provided in Table 1, wherein the individual is classified as having ovarian cancer, or the likelihood of the individual having ovarian cancer is determined, based on the at least one biomarker value.
-
公开(公告)号：US08409795B2
公开(公告)日：2013-04-02
申请号：US12175388
申请日：2008-07-17
申请人： Daniel J. Schneider , Sheri K. Wilcox , Dominic Zichi , Dan Nieuwlandt , Jeff Carter , Larry Gold
发明人： Daniel J. Schneider , Sheri K. Wilcox , Dominic Zichi , Dan Nieuwlandt , Jeff Carter , Larry Gold
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6811 , C12N15/1048 , C12N15/111 , C12N15/115 , C12N2310/16 , C12N2320/13 , C12Q2541/101 , C12Q2525/205 , C12Q2525/117
摘要： The present disclosure describes improved SELEX methods for generating nucleic acid ligands that are capable of binding to target molecules and improved photoSELEX methods for generating photoreactive nucleic acid ligands that are capable of both binding and covalently crosslinking to target molecules. The disclosure further describes nucleic acid libraries having expanded physical and chemical properties and their use in SELEX and photoSELEX; methods for increasing the crosslinking efficiencies of photoaptamers; methods for producing photoaptamers having selective modifications that enhance functionality and minimize non-specific photoreactions; and methods for generating truncated nucleic acid ligands from nucleic acid ligands of longer length. The disclosure further describes aptamers and photoaptamers obtained by using any of the foregoing.
摘要翻译： 本公开描述了用于产生能够结合靶分子的核酸配体的改进的SELEX方法和用于产生能够与靶分子结合和共价交联的光反应性核酸配体的改进的photoSELEX方法。 本公开进一步描述了具有扩展的物理和化学性质及其在SELEX和photoSELEX中的用途的核酸文库; 提高光催化剂交联效率的方法; 制备具有增强功能并使非特异性光反应最小化的选择性修饰的光致抗体的方法; 以及用于从较长长度的核酸配体产生截短的核酸配体的方法。 本公开进一步描述了通过使用上述任何一种获得的适体和光致抗体。
-
公开(公告)号：US20120143805A1
公开(公告)日：2012-06-07
申请号：US13390648
申请日：2010-03-05
申请人： Larry Gold , Edward N. Brody , Rachel M. Ostroff , Dominic Zichi , Alex A.E. Stewart , Michael Riel-Mehan , Mark Messenbaugh , Randee S. Schwartz , Jeffery Walker , Stephen Alaric Williams , Malti Nikrad
发明人： Larry Gold , Edward N. Brody , Rachel M. Ostroff , Dominic Zichi , Alex A.E. Stewart , Michael Riel-Mehan , Mark Messenbaugh , Randee S. Schwartz , Jeffery Walker , Stephen Alaric Williams , Malti Nikrad
CPC分类号： G01N33/57423 , G01N33/574 , G01N2800/60 , G16H50/20
摘要： The present disclosure includes biomarkers, methods, devices, reagents, systems, and kits for the detection and diagnosis of cancer. In one aspect, the disclosure provides biomarkers that can be used alone or in various combinations to diagnose cancer. In another aspect, methods are provided for diagnosing cancer in an individual, where the methods include detecting, in a biological sample from an individual, at least one biomarker value corresponding to at least one biomarker selected from the group of biomarkers provided in Table 47, wherein the individual is classified as having cancer, or the likelihood of the individual having cancer is determined, based on the at least one biomarker value.
摘要翻译： 本公开包括用于癌症的检测和诊断的生物标志物，方法，装置，试剂，系统和试剂盒。 一方面，本公开提供可以单独使用或以各种组合使用以诊断癌症的生物标志物。 在另一方面，提供了用于诊断个体癌症的方法，其中所述方法包括在来自个体的生物样品中检测至少一个对应于选自表47中提供的生物标志物组的生物标志物的生物标记值， 其中所述个体基于所述至少一种生物标志物值被分类为具有癌症或确定个体患有癌症的可能性。
-
-
-
-
-
-
-
-
-
搜索结果
223 条记录 (耗时 0.046 秒)
