公开(公告)号：US20130195831A1

公开(公告)日：2013-08-01

申请号：US13826598

申请日：2013-03-14

申请人： TRUSTEES OF TUFTS COLLEGE ,  Eidgenossisches Technische Hochschule (The Swiss Federal Institute of Technology)

发明人： David L. Kaplan ,  Lorenz Meinel

IPC分类号： A61K47/42

CPC分类号： A61K47/42 ,  A61K9/0002 ,  A61K9/7007 ,  A61K31/00 ,  A61K47/46

摘要： The present invention provides for novel sustained release silk-based delivery systems. The invention further provides methods for producing such formulations. In general, a silk fibroin solution is combined with a therapeutic agent to form a silk fibroin article. The article is then treated in such a way as to alter its conformation. The change in conformation increases its crystallinity or liquid crystallinity, thus controlling the release of a therapeutic agent from the formulation. This can be accomplished as single material carriers or in a layer-by-layer fashion to load different therapeutic agents or different concentrations of these agents in each layer.

公开(公告)号：US10548981B2

公开(公告)日：2020-02-04

申请号：US14095366

申请日：2013-12-03

申请人： TRUSTEES OF TUFTS COLLEGE ,  EIDGENOSSISCHES TECHNISCHE HOCHSCHULE (THE SWISS FEDERAL INSTITUTE OF TECHNOLOGY)

发明人： David L. Kaplan ,  Lorenz Meinel

IPC分类号： A61K9/24 ,  A61K9/00 ,  A61K47/42

摘要： The present invention provides for novel sustained release silk-based delivery systems. The invention further provides methods for producing such formulations. In general, a silk fibroin solution is combined with a therapeutic agent to form a silk fibroin article. The article is then treated in such a way as to alter its conformation. The change in conformation increases its crystallinity or liquid crystallinity, thus controlling the release of a therapeutic agent from the formulation. This can be accomplished as single material carriers or in a layer-by-layer fashion to load different therapeutic agents or different concentrations of these agents in each layer.

公开(公告)号：US20140093580A1

公开(公告)日：2014-04-03

申请号：US14095366

申请日：2013-12-03

申请人： Eidgenossisches Technische Hochschule (The Swiss Federal Institute Of Technology) ,  Trustees Of Tufts College

发明人： David L. Kaplan ,  Lorenz Meinel

IPC分类号： A61K47/42

摘要： The present invention provides for novel sustained release silk-based delivery systems. The invention further provides methods for producing such formulations. In general, a silk fibroin solution is combined with a therapeutic agent to form a silk fibroin article. The article is then treated in such a way as to alter its conformation. The change in conformation increases its crystallinity or liquid crystallinity, thus controlling the release of a therapeutic agent from the formulation. This can be accomplished as single material carriers or in a layer-by-layer fashion to load different therapeutic agents or different concentrations of these agents in each layer.

摘要翻译： 本发明提供了新颖的持续释放的基于丝的递送系统。 本发明还提供了生产这种制剂的方法。 通常，将丝素蛋白溶液与治疗剂组合以形成丝素蛋白制品。 然后对该文章进行处理，以改变其构象。 构象的变化增加其结晶度或液晶性，从而控制治疗剂从制剂中的释放。 这可以作为单一材料载体或以逐层方式实现，以在每个层中加载不同的治疗剂或不同浓度的这些试剂。

公开(公告)号：US20240132836A1

公开(公告)日：2024-04-25

申请号：US18352366

申请日：2023-07-14

申请人： Trustees of Tufts College

发明人： David L. Kaplan ,  Jugal Kishore Sahoo ,  Onur Hasturk ,  Jaewon Choi

IPC分类号： C12N5/00

CPC分类号： C12N5/0068 ,  C12N2533/50 ,  C12N2537/10

摘要： Saccharide-substituted silk fibroin compositions as well as methods for making and using the same are provided. The compositions can include at least one saccharide coupled to silk fibroin. The coupling can be via various residues on the silk fibroin.

公开(公告)号：US11576862B2

公开(公告)日：2023-02-14

申请号：US15646601

申请日：2017-07-11

申请人： Trustees of Tufts College

发明人： David L. Kaplan ,  Tuna Yucel ,  Xiaoqin Wang ,  Michael Lovett

IPC分类号： A61K9/16 ,  A61K9/00 ,  A61K31/13 ,  C07K16/22 ,  A61K47/38

摘要： Provided herein relates to methods and compositions for preparing a silk microsphere and the resulting silk microsphere. In some embodiments, the methods and compositions described herein are all aqueous, which can be used for encapsulating an active agent in a silk microsphere, while maintaining activity of the active agent during processing. In some embodiments, the resulting silk microsphere can be used for sustained delivery of an active agent encapsulated therein.

公开(公告)号：US20220299494A1

公开(公告)日：2022-09-22

申请号：US17753312

申请日：2020-08-28

申请人： Trustees of Tufts College

发明人： David L. Kaplan ,  Qiaobing Xu

IPC分类号： G01N33/15 ,  C12N5/071 ,  A61K9/51 ,  G01N33/50

摘要： The present disclosure provides a method for screening drug delivery vehicles for use in delivering cargo via oral delivery. The method includes introducing a drug delivery vehicle comprising an imaging agent into a lumen of an artificial intestine system composed of a scaffold matrix material. The scaffold matrix material includes an interconnected network of pores, intestinal epithelial cells positioned on an inner surface of the lumen, and human-based cells positioned within the pores and surrounding the intestinal epithelial cells. The method includes maintaining the artificial intestine system in physiologically relevant conditions for a predetermined length of time, and detecting a color change induced by the imaging agent within at least a portion of the human-based cells.

公开(公告)号：US11266339B2

公开(公告)日：2022-03-08

申请号：US16408122

申请日：2019-05-09

申请人： Trustees of Tufts College

发明人： David L. Kaplan ,  Lee W. Tien ,  Gary G. Leisk ,  Tim Jia-Ching Lo ,  Cinzia Metallo ,  Fiorenzo Omenetto

IPC分类号： A61L31/10 ,  A61B5/291 ,  A61B5/00 ,  A61L27/22 ,  A61L27/36 ,  A61L31/04 ,  A61L31/14 ,  A61N1/05 ,  A61L31/16

摘要： Provided herein relates to implantable devices and systems with dynamic silk coatings. In some embodiments, the dynamic silk coatings can be formed in situ or in vivo.

公开(公告)号：US20210333178A1

公开(公告)日：2021-10-28

申请号：US17302069

申请日：2021-04-22

申请人： Trustees of Tufts College

发明人： David L. Kaplan ,  Rachael N. Parker

IPC分类号： G01N1/30

摘要： Compositions and methods for improved histological analysis of organoids are disclosed. The compositions and methods include silk-elastin-like polymers have temperature-induced shape change properties.

公开(公告)号：US20200282106A1

公开(公告)日：2020-09-10

申请号：US16406538

申请日：2019-05-08

申请人： Trustees of Tufts College

发明人： David L. Kaplan ,  Ung-Jin Kim ,  Jaehyung Park ,  Hyoung-Joon Jin

IPC分类号： A61L27/22 ,  A61L27/36 ,  A61L27/38 ,  A61L27/52 ,  A61L27/56 ,  C07K14/435 ,  C08J3/07 ,  C08J3/075 ,  C08J5/18 ,  C08J9/00 ,  C08J9/26 ,  C08J9/28 ,  C08L89/00 ,  D01D5/00 ,  D01F4/02 ,  B29C39/00 ,  B29C39/20 ,  B29C55/00 ,  B29D7/01

摘要： The present invention provides for concentrated aqueous silk fibroin solutions and an all-aqueous mode for preparation of concentrated aqueous fibroin solutions that avoids the use of organic solvents, direct additives, or harsh chemicals. The invention further provides for the use of these solutions in production of materials, e.g., fibers, films, foams, meshes, scaffolds and hydrogels.

公开(公告)号：US10736943B2

公开(公告)日：2020-08-11

申请号：US13888605

申请日：2013-05-07

申请人： TRUSTEES OF TUFTS COLLEGE

发明人： David L. Kaplan ,  Xiaoqin Wang

IPC分类号： A61K9/16 ,  A61K9/19 ,  C07K14/435 ,  A61K38/44 ,  A61K47/42 ,  A61K47/34 ,  A61K9/50 ,  A61K31/7076 ,  A61K9/127

摘要： A method was developed to prepare silk fibroin microspheres using lipid vesicles as templates to efficiently load therapeutic agents in active form for controlled release. The lipids are subsequently removed through the use of a dehydration agent, such as methanol or sodium chloride, resulting in β-sheet structure dominant silk microsphere structures having about 2 μm in diameter. The therapeutic agent can be entrapped in the silk microspheres and used in pharmaceutical formulations for controlled-release treatments.