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    METHOD FOR UTILIZING MONOTERPENE GLYCOSYLTRANSFERASE
    3.
    发明申请
    METHOD FOR UTILIZING MONOTERPENE GLYCOSYLTRANSFERASE 有权
    利用单稳态糖蛋白酶的方法

    公开(公告)号：US20140020137A1

    公开(公告)日：2014-01-16

    申请号：US13997683

    申请日：2011-12-27

    申请人： Eiichiro Ono ,  Nobuo Tsuruoka

    发明人： Eiichiro Ono ,  Nobuo Tsuruoka

    IPC分类号： C12P19/44 ,  C12N9/10

    CPC分类号： C12P19/44 ,  A23L27/36 ,  A61K8/602 ,  A61K8/64 ,  A61Q19/00 ,  C12N9/1048 ,  C12N9/1051 ,  C12N15/8257 ,  C12P19/18 ,  C12Y204/01 ,  Y02P20/52

    摘要： The object of the present invention is to provide a novel method for producing a terpene 8-glycoside.The present invention provides a method for producing a terpene 8-glycoside by means of glycosyltransferase acting on the 8-position of terpenes. The present invention provides a transformant transformed with a gene for the glycosyltransferase acting on the 8-position of terpenes and a method for producing such a transformant. The present invention provides a plant modified to suppress the expression of a protein having glycosylation activity on the 8-position of a monoterpene compound.

    摘要翻译： 本发明的目的是提供一种制备萜烯8-糖苷的新方法。 本发明提供了通过作用于萜烯8位的糖基转移酶来制备萜烯8-糖苷的方法。 本发明提供了用作用于萜烯8位的糖基转移酶的基因转化的转化体及其生产方法。 本发明提供了修饰以抑制在单萜化合物的8-位具有糖基化活性的蛋白质的表达的植物。

    Gene Encoding an Enzyme for Catalyzing Biosynthesis of Lignan, and Use Thereof
    4.
    发明申请
    Gene Encoding an Enzyme for Catalyzing Biosynthesis of Lignan, and Use Thereof 有权
    编码催化木质素生物合成酶的基因及其用途

    公开(公告)号：US20070271624A1

    公开(公告)日：2007-11-22

    申请号：US10573885

    申请日：2004-09-29

    申请人： Eiichiro Ono ,  Yoshikazu Tanaka

    发明人： Eiichiro Ono ,  Yoshikazu Tanaka

    IPC分类号： A01H5/00 ,  C07H21/04 ,  C12Q1/68 ,  C12P21/06 ,  C12N9/02 ,  C12N9/06

    CPC分类号： C12P17/181 ,  C12N9/0071 ,  C12N15/8243

    摘要： The present invention provides an enzyme that catalyzes the reaction producing piperitol from pinoresinol, and a reaction producing sesamin from piperitol. The invention also provides a gene that encodes such enzyme. Further, the invention provides a vector and transformant including a gene encoding the enzyme, and a producing method of the protein using the transformant.

    摘要翻译： 本发明提供了一种催化由哌嗪醇产生胡椒醇的反应的酶，以及由胡椒醇生成芝麻素的反应。 本发明还提供了编码这种酶的基因。 此外，本发明提供了包含编码该酶的基因的载体和转化体，以及使用该转化体的蛋白质的制造方法。

    Polynucleotide encoding UDP-glucuronyl transferase
    5.
    发明授权
    Polynucleotide encoding UDP-glucuronyl transferase 失效
    编码UDP-葡糖醛酸转移酶的多核苷酸

    公开(公告)号：US08628938B2

    公开(公告)日：2014-01-14

    申请号：US12678161

    申请日：2008-09-29

    申请人： Eiichiro Ono ,  Akio Noguchi ,  Yuko Fukui ,  Masako Mizutani

    发明人： Eiichiro Ono ,  Akio Noguchi ,  Yuko Fukui ,  Masako Mizutani

    IPC分类号： C12P21/06

    CPC分类号： C12N9/10 ,  C12N9/1051 ,  C12Y204/01017

    摘要： The present invention provides a novel UDP-glucuronosyltransferase and a polynucleotide encoding the same, for example, a polynucleotide comprising nucleotides 1 to 1359 of SEQ ID NO: 4, nucleotides 1 to 1365 of SEQ ID NO: 10, nucleotides 1 to 1371 of SEQ ID NO: 12, and nucleotides 1 to 1371 of SEQ ID NO: 22; or a polynucleotide encoding a protein having the amino acid sequence of SEQ ID NOS: 5, 11, 13 or 23. The invention provides a novel UDP-glucuronosyltransferase with a broad substrate specificity.

    摘要翻译： 本发明提供新的UDP-葡糖醛酸糖基转移酶和编码该多核苷酸的多核苷酸，例如包含SEQ ID NO：4的核苷酸1至1359，SEQ ID NO：10的核苷酸1至1365，SEQ ID NO：10的核苷酸1至1371，SEQ ID NO： SEQ ID NO：12和SEQ ID NO：22的核苷酸1至1371; 或编码具有SEQ ID NO：5,11,13或23的氨基酸序列的蛋白质的多核苷酸。本发明提供了具有广泛底物特异性的新型UDP-葡糖醛酸基转移酶。

    LIGNAN HYDROXYLASE
    6.
    发明申请
    LIGNAN HYDROXYLASE 失效
    LIGNAN羟化酶

    公开(公告)号：US20110104360A1

    公开(公告)日：2011-05-05

    申请号：US12745292

    申请日：2008-12-17

    申请人： Eiichiro Ono ,  Asako Okada ,  Yuko Fukui

    发明人： Eiichiro Ono ,  Asako Okada ,  Yuko Fukui

    IPC分类号： C12N15/63 ,  C12N9/02 ,  C07H21/04 ,  C12N5/10 ,  A01H5/00 ,  C12P21/02 ,  C12P1/00 ,  C12N15/82 ,  C07D493/04 ,  C12P17/18 ,  A23L1/305

    CPC分类号： C12P17/181 ,  A23L33/18 ,  C07D493/04 ,  C12N9/0004 ,  C12N9/0071 ,  C12N15/8255

    摘要： The invention provides an enzyme having a lignan-hydroxylating activity, particularly an enzyme capable of catalyzing the reaction of transferring a hydroxyl group to a lignan, an enzyme capable of catalyzing the hydroxylation of piperitol to 9-hydroxylpiperitol or pinoresinol to 9-hydroxylpinoresinol. The invention provides a polypeptide having a lignan-hydroxylating activity; a polynucleotide encoding the same; a vector or transformant comprising the polynucleotide; a method for producing a polypeptide having a lignan-hydroxylating activity which comprises using the transformant; and so on. The transformant wherein the polynucleotide is expressibly introduced is useful for the hydroxylation of a lignan or for the production of a product using the same in the food sector and a variety of industry sectors.

    摘要翻译： 本发明提供了具有木酚素羟化活性的酶，特别是能够催化将羟基转移到木脂素的反应的酶，能够催化胡椒醇羟基化为9-羟基哌啶醇或者尖晶石酚至9-羟基苯甲醇的酶。 本发明提供了具有木酚素羟化活性的多肽; 编码该多核苷酸的多核苷酸; 包含所述多核苷酸的载体或转化体; 一种生产具有木酚素羟化活性的多肽的方法，其包括使用所述转化体; 等等。 其中可表达引入的多核苷酸的转化体可用于木质素的羟基化或用于在食品部门和各种工业部门中使用其的产品的生产。

    Gene encoding an enzyme for catalyzing biosynthesis of lignan, and use thereof
    7.
    发明授权
    Gene encoding an enzyme for catalyzing biosynthesis of lignan, and use thereof 有权
    编码用于催化木脂素生物合成的酶的基因及其用途

    公开(公告)号：US07811823B2

    公开(公告)日：2010-10-12

    申请号：US10573885

    申请日：2004-09-29

    申请人： Eiichiro Ono ,  Yoshikazu Tanaka

    发明人： Eiichiro Ono ,  Yoshikazu Tanaka

    IPC分类号： C12N15/83 ,  C12N15/11 ,  C12N15/00 ,  A01H5/00

    CPC分类号： C12P17/181 ,  C12N9/0071 ,  C12N15/8243

    摘要： The present invention provides an enzyme that catalyzes the reaction producing piperitol from pinoresinol, and a reaction producing sesamin from piperitol. The invention also provides a gene that encodes such enzyme. Further, the invention provides a vector and transformant including a gene encoding the enzyme, and a producing method of the protein using the transformant.

    摘要翻译： 本发明提供了一种催化由哌嗪醇产生胡椒醇的反应的酶，以及由胡椒醇生成芝麻素的反应。 本发明还提供了编码这种酶的基因。 此外，本发明提供了包含编码该酶的基因的载体和转化体，以及使用该转化体的蛋白质的制造方法。

    Lignan glycosidase and utilization of the same
    9.
    发明授权
    Lignan glycosidase and utilization of the same 有权
    木糖苷酶和利用率相同

    公开(公告)号：US07935802B2

    公开(公告)日：2011-05-03

    申请号：US11666157

    申请日：2005-11-02

    申请人： Eiichiro Ono ,  Yoshikazu Tanaka

    发明人： Eiichiro Ono ,  Yoshikazu Tanaka

    IPC分类号： C07H21/04 ,  C12N9/42 ,  C12P21/02 ,  C07D307/80

    CPC分类号： C12N9/2402 ,  C12N9/1048 ,  C12N15/8245 ,  C12N15/8255 ,  C12P19/60

    摘要： The present invention provides an enzyme having the lignan glycosidation activity by identifying the enzyme that is involved in the production of lignan glycosides, identifying the amino acid sequence of the enzyme polypeptide and the base sequence for a polynucleotide encoding the polypeptide, and based on the information of these sequences, preparing the transformants capable of producing the lignan glycosides.

    摘要翻译： 本发明通过鉴定参与木素糖苷生产的酶，鉴定酶多肽的氨基酸序列和编码多肽的多核苷酸的碱基序列，提供了具有木素素糖苷结合活性的酶，并且基于该信息 的这些序列，制备能够产生木脂糖苷的转化体。

    UDP-GLUCURONYL TRANSFERASE AND POLYNUCLEOTIDE ENCODING THE SAME
    10.
    发明申请
    UDP-GLUCURONYL TRANSFERASE AND POLYNUCLEOTIDE ENCODING THE SAME 失效
    UDP-GLUCURONYL转移和编码它们的多核苷酸

    公开(公告)号：US20100323402A1

    公开(公告)日：2010-12-23

    申请号：US12678161

    申请日：2008-09-29

    申请人： Eiichiro Ono ,  Akio Noguchi ,  Yuko Fukui ,  Masako Mizutani

    发明人： Eiichiro Ono ,  Akio Noguchi ,  Yuko Fukui ,  Masako Mizutani

    IPC分类号： C12P19/60 ,  C07H21/04 ,  C07H21/00 ,  C12N9/10 ,  C12N15/63 ,  C12N1/21

    CPC分类号： C12N9/10 ,  C12N9/1051 ,  C12Y204/01017

    摘要： The present invention provides a novel UDP-glucuronosyltransferase and a polynucleotide encoding the same (for example, a polynucleotide comprising a polynucleotide consisting of one nucleotide sequence selected from the group consisting of the nucleotide sequence at positions 1 to 1359 in the nucleotide sequence represented by SEQ ID NO: 4, the nucleotide sequence at positions 1 to 1365 in the nucleotide sequence represented by SEQ ID NO: 10, the nucleotide sequence at positions 1 to 1371 in the nucleotide sequence represented by SEQ ID NO: 12, and the nucleotide sequence at positions 1 to 1371 in the nucleotide sequence represented by SEQ ID NO: 22; or a polynucleotide comprising a polynucleotide encoding a protein having one amino acid sequence selected from the group consisting of SEQ ID NOS: 5, 11, 13 and 23), etc. This provides a novel UDP-glucuronosyltransferase with a broad substrate specificity.

    摘要翻译： 本发明提供新的UDP-葡糖醛酸糖基转移酶及其编码的多核苷酸（例如，包含多核苷酸的多核苷酸，所述多核苷酸由选自SEQ ID NO：1所示的核苷酸序列中的1〜1359位的核苷酸序列的一个核苷酸序列组成 SEQ ID NO：10所示核苷酸序列中的1〜1365位核苷酸序列，SEQ ID NO：12所示核苷酸序列中1〜1371位核苷酸序列， 或包含编码具有选自SEQ ID NO：5,11,13和23的一个氨基酸序列的蛋白质的多核苷酸的多核苷酸等），等等。 这提供了具有广泛底物特异性的新型UDP-葡糖醛酸基转移酶。