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1.发明授权Plasmid-encoded neurotoxin genes in Clostridium botulinum serotype A subtypes 有权肉毒毒素肉毒杆菌血清型A亚型中的质粒编码的神经毒素基因公开(公告)号：US08435759B2
公开(公告)日：2013-05-07
申请号：US12664800
申请日：2008-06-27
CPC分类号： C12N9/52 , C07K14/33 , C12N15/74 , C12Y304/24069
摘要： The present invention provides a novel isolated plasmid, wherein the plasmid is a native plasmid found in unique C. botulinum type A strains and encode either BoNT/A3 or BoNT/A4 and BoNT/B. The present invention also provides a method of obtaining a plasmid-encoded botulinum neurotoxin and botulinum neurotoxin complex comprising the step of isolating a plasmid encoding the cntA/A or cntA/B neurotoxin gene and genes encoding protein components of the toxin complex from a C. botulinum type A strain. The inventors performed comparative analyzes of representative BoNT/A subtype strains by pulsed-field gel electrophoresis (PFGE) and Southern hybridizations with probes specific for the BoNT/A and B genes, cntA/A and cntA/B. Unexpectedly, the inventors determined that the genes encoding BoNT/A3 in the A3 strain, and BoNT/A4 and BoNT/B in the A4 strain, are on plasmids.
摘要翻译： 本发明提供了一种新的分离质粒，其中质粒是在独特的A型肉毒杆菌中发现的天然质粒，并且编码BoNT / A3或BoNT / A4和BoNT / B。 本发明还提供了获得质粒编码的肉毒杆菌神经毒素和肉毒杆菌神经毒素复合物的方法，包括分离编码cntA / A或cntA / B神经毒素基因的质粒和编码来自C的毒素复合物的蛋白质组分的基因的步骤。 A型肉毒杆菌。 本发明人通过脉冲场凝胶电泳（PFGE）和针对BoNT / A和B基因cntA / A和cntA / B特异性探针的Southern杂交进行了代表性的BoNT / A亚型菌株的比较分析。 出人意料的是，本发明人确定了A3菌株中编码BoNT / A3的基因以及A4菌株中的BoNT / A4和BoNT / B在质粒上。
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2.发明申请PLASMID-ENCODED NEUROTOXIN GENES IN CLOSTRIDIUM BOTULINUM SEROTYPE A SUBTYPES 有权UM ID ES ES ES ES ES ES ES ES ES ES ES ES ES ES ES ES ES ES ES ES ES公开(公告)号：US20100311118A1
公开(公告)日：2010-12-09
申请号：US12664800
申请日：2008-06-27
CPC分类号： C12N9/52 , C07K14/33 , C12N15/74 , C12Y304/24069
摘要： The present invention provides a novel isolated plasmid, wherein the plasmid is a native plasmid found in unique C. botulinum type A strains and encode either BoNT/A3 or BoNT/A4 and BoNT/B. The present invention also provides a method of obtaining a plasmid-encoded botulinum neurotoxin and botulinum neurotoxin complex comprising the step of isolating a plasmid encoding the cntA/A or cntA/B neurotoxin gene and genes encoding protein components of the toxin complex from a C. botulinum type A strain. The inventors performed comparative analyses of representative BoNT/A subtype strains by pulsed-field gel electrophoresis (PFGE) and Southern hybridizations with probes specific for the BoNT/A and B genes, cntA/A and cntA/B. Unexpectedly, the inventors determined that the genes encoding BoNT/A3 in the A3 strain, and BoNT/A4 and BoNT/B in the A4 strain, are on plasmids.
摘要翻译： 本发明提供了一种新的分离质粒，其中质粒是在独特的A型肉毒杆菌中发现的天然质粒，并且编码BoNT / A3或BoNT / A4和BoNT / B。 本发明还提供了获得质粒编码的肉毒杆菌神经毒素和肉毒杆菌神经毒素复合物的方法，包括分离编码cntA / A或cntA / B神经毒素基因的质粒和编码来自C的毒素复合物的蛋白质组分的基因的步骤。 A型肉毒杆菌。 本发明人通过脉冲场凝胶电泳（PFGE）和针对BoNT / A和B基因cntA / A和cntA / B特异性探针的Southern杂交进行了代表性的BoNT / A亚型菌株的比较分析。 出人意料的是，本发明人确定了A3菌株中编码BoNT / A3的基因以及A4菌株中的BoNT / A4和BoNT / B在质粒上。
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3.发明申请Method of detecting botulinum neurotoxin and antibodies that neutralize botulinum neurotoxin action 有权检测肉毒杆菌神经毒素的方法和中和肉毒杆菌神经毒素作用的抗体公开(公告)号：US20120164657A1
公开(公告)日：2012-06-28
申请号：US12291411
申请日：2008-11-10
申请人： Eric A. Johnson , Sabine Pellett , William H. Tepp , Gary E. Borodic , David J. Beebe , Amy Paguirigan
发明人： Eric A. Johnson , Sabine Pellett , William H. Tepp , Gary E. Borodic , David J. Beebe , Amy Paguirigan
IPC分类号： G01N33/566 , C12N5/071 , C12Q1/02
CPC分类号： C12Q1/37 , G01N2333/33
摘要： A highly sensitive method of analyzing a sample for the presence or activity of botulinum neurotoxin (BoNT) or antibodies specific for botulinum neurotoxin is disclosed. In one embodiment, the method comprises the steps of preparing primary non-human mammalian or avian spinal cord cells, and exposing the cells to a test sample, in parallel with a control sample, and examining the extent of cleavage of the intracellular neuronal target protein in both the test and control sample.
摘要翻译： 公开了一种分析样品对肉毒杆菌神经毒素（BoNT）或特异于肉毒杆菌神经毒素的抗体的存在或活性的高度灵敏的方法。 在一个实施方案中，该方法包括以下步骤：与对照样品平行地制备初级非人哺乳动物或禽脊髓细胞，并将细胞暴露于测试样品，并检查细胞内神经元靶蛋白的切割程度 在测试和对照样品中。
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4.发明授权Regulation of specific spinal neurons regulating pain transmission via chimeric toxins 有权调节通过嵌合毒素调节疼痛传播的特定脊髓神经元公开(公告)号：US09447405B2
公开(公告)日：2016-09-20
申请号：US14181241
申请日：2014-02-14
申请人： Eric A. Johnson , Sabine Pellett , William H. Tepp , Tony L. Yaksh , Marc Marino , Qinghao Xu
发明人： Eric A. Johnson , Sabine Pellett , William H. Tepp , Tony L. Yaksh , Marc Marino , Qinghao Xu
IPC分类号： A61K38/16 , A61K39/08 , C07K14/33 , C12N9/96 , C07K7/22 , A61K47/48 , C07K14/575 , C07K14/70 , C07K14/72 , A61K38/48
CPC分类号： A61K47/62 , A61K9/0085 , A61K38/164 , A61K38/4893 , C07K7/22 , C07K14/33 , C07K14/57545 , C07K14/70 , C07K14/72 , C07K2319/01 , C07K2319/33 , C07K2319/55 , C12N9/96 , C12Y304/24069
摘要： A chimeric toxin is disclosed comprising a peptide ligand specifically targeting neurons involved in pain processing; and a clostridial neurotoxin light chain, wherein the ligand is linked to the light chain. The methods of preparing such chimeric toxin and the method of using the chimeric toxin to regulate pain transmission are also disclosed.
摘要翻译： 公开了一种嵌合毒素，其包含特异性靶向涉及疼痛加工的神经元的肽配体; 和梭菌神经毒素轻链，其中所述配体与轻链连接。 还公开了制备这种嵌合毒素的方法和使用嵌合毒素调节疼痛传递的方法。
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5.发明授权Method of detecting botulinum neurotoxin and antibodies that neutralize botulinum neurotoxin action 有权检测肉毒杆菌神经毒素的方法和中和肉毒杆菌神经毒素作用的抗体公开(公告)号：US08940477B2
公开(公告)日：2015-01-27
申请号：US12291411
申请日：2008-11-10
申请人： Eric A. Johnson , Sabine Pellett , William H. Tepp , Gary E. Borodic , David J. Beebe , Amy Paguirigan
发明人： Eric A. Johnson , Sabine Pellett , William H. Tepp , Gary E. Borodic , David J. Beebe , Amy Paguirigan
IPC分类号： C12Q1/00 , C12Q1/68 , G01N33/567 , C12Q1/37
CPC分类号： C12Q1/37 , G01N2333/33
摘要： A highly sensitive method of analyzing a sample for the presence or activity of botulinum neurotoxin (BoNT) or antibodies specific for botulinum neurotoxin is disclosed. In one embodiment, the method comprises the steps of preparing primary non-human mammalian or avian spinal cord cells, and exposing the cells to a test sample, in parallel with a control sample, and examining the extent of cleavage of the intracellular neuronal target protein in both the test and control sample.
摘要翻译： 公开了一种分析样品对肉毒杆菌神经毒素（BoNT）或特异于肉毒杆菌神经毒素的抗体的存在或活性的高度灵敏的方法。 在一个实施方案中，该方法包括以下步骤：与对照样品平行地制备初级非人哺乳动物或禽脊髓细胞，并将细胞暴露于测试样品，并检查细胞内神经元靶蛋白的切割程度 在测试和对照样品中。
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公开(公告)号：US20140255376A1
公开(公告)日：2014-09-11
申请号：US14181241
申请日：2014-02-14
申请人： Eric A. Johnson , Sabine Pellett , William H. Tepp , Tony L. Yaksh , Marc Marino , Qinghao Xu
发明人： Eric A. Johnson , Sabine Pellett , William H. Tepp , Tony L. Yaksh , Marc Marino , Qinghao Xu
CPC分类号： A61K47/62 , A61K9/0085 , A61K38/164 , A61K38/4893 , C07K7/22 , C07K14/33 , C07K14/57545 , C07K14/70 , C07K14/72 , C07K2319/01 , C07K2319/33 , C07K2319/55 , C12N9/96 , C12Y304/24069
摘要： A chimeric toxin is disclosed comprising a peptide ligand specifically targeting neurons involved in pain processing; and a clostridial neurotoxin light chain, wherein the ligand is linked to the light chain. The methods of preparing such chimeric toxin and the method of using the chimeric toxin to regulate pain transmission are also disclosed.
摘要翻译： 公开了一种嵌合毒素，其包含特异性靶向涉及疼痛加工的神经元的肽配体; 和梭菌神经毒素轻链，其中所述配体与轻链连接。 还公开了制备这种嵌合毒素的方法和使用嵌合毒素调节疼痛传递的方法。
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