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公开(公告)号：US08945835B2
公开(公告)日：2015-02-03
申请号：US13444654
申请日：2012-04-11
申请人： Eric Hans Vermaas , Graham John Worsley , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , Geoffrey Paul Smith , Vincent Peter Smith , Tobias William Barr Ost , David Bentley
发明人： Eric Hans Vermaas , Graham John Worsley , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , Geoffrey Paul Smith , Vincent Peter Smith , Tobias William Barr Ost , David Bentley
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6869 , C12Q2565/518 , C12Q2537/149
摘要： The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.
摘要翻译： 本发明提供了双链多核苷酸模板的成对测序的方法，该方法导致多核苷酸模板的两个不同和分离的区域中的核苷酸序列的顺序测定。
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公开(公告)号：US20130165327A1
公开(公告)日：2013-06-27
申请号：US13444654
申请日：2012-04-11
申请人： Eric Hans Vermaas , Graham John Worsley , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , Geoffrey Paul Smith , Vincent Peter Smith , Tobias William Barr Ost , David Bentley
发明人： Eric Hans Vermaas , Graham John Worsley , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , Geoffrey Paul Smith , Vincent Peter Smith , Tobias William Barr Ost , David Bentley
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6869 , C12Q2565/518 , C12Q2537/149
摘要： The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.
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公开(公告)号：US08192930B2
公开(公告)日：2012-06-05
申请号：US12223759
申请日：2007-02-08
申请人： Eric Hans Vermaas , Graham John Worsley , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , Geoffrey Paul Smith , Vincent Peter Smith , Tobias William Barr Ost , David Bentley
发明人： Eric Hans Vermaas , Graham John Worsley , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , Geoffrey Paul Smith , Vincent Peter Smith , Tobias William Barr Ost , David Bentley
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6869 , C12Q2565/518 , C12Q2537/149
摘要： The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.
摘要翻译： 本发明提供了双链多核苷酸模板的成对测序的方法，该方法导致多核苷酸模板的两个不同和分离的区域中的核苷酸序列的顺序测定。
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公开(公告)号：US20110009276A1
公开(公告)日：2011-01-13
申请号：US12223759
申请日：2007-02-08
申请人： Eric Hans Vermaas , Graham John Worsley , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , Geoffrey Paul Smith , Vincent Peter Smith , Tobias Willaim Barr Ost , David Bentley
发明人： Eric Hans Vermaas , Graham John Worsley , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , Geoffrey Paul Smith , Vincent Peter Smith , Tobias Willaim Barr Ost , David Bentley
IPC分类号： C40B30/00
CPC分类号： C12Q1/6874 , C12Q1/6869 , C12Q2565/518 , C12Q2537/149
摘要： The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.
摘要翻译： 本发明提供了双链多核苷酸模板的成对测序的方法，该方法导致多核苷酸模板的两个不同和分离的区域中的核苷酸序列的顺序测定。
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公开(公告)号：US20140349891A1
公开(公告)日：2014-11-27
申请号：US14166670
申请日：2014-01-28
申请人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
发明人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
IPC分类号： C12Q1/68
CPC分类号： B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
摘要： The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction,
摘要翻译： 本发明涉及产生用于核酸测序反应的模板的方法，其包括：提供至少一个双链核酸分子，其中双链核酸分子的两条链在5'末端连接到固体支持物上 切割双链核酸分子的一条或两条链，并使切割的链进行变性条件以除去未连接到固体支持物上的部分切割的链，从而产生部分或基本上 用于核酸测序反应的单链模板，
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公开(公告)号：US09085802B2
公开(公告)日：2015-07-21
申请号：US14166670
申请日：2014-01-28
申请人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
发明人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
IPC分类号： C12Q1/68
CPC分类号： B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
摘要： The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
摘要翻译： 本发明涉及产生用于核酸测序反应的模板的方法，其包括：提供至少一个双链核酸分子，其中双链核酸分子的两条链在5'末端连接到固体支持物 切割双链核酸分子的一条或两条链，并使切割的链进行变性条件以除去未连接到固体支持物上的部分切割的链，从而产生部分或基本上 用于核酸测序反应的单链模板。
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公开(公告)号：US08715966B2
公开(公告)日：2014-05-06
申请号：US13450920
申请日：2012-04-19
申请人： Liu Xiaohai , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
发明人： Liu Xiaohai , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
CPC分类号： B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
摘要： The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
摘要翻译： 本发明涉及产生用于核酸测序反应的模板的方法，其包括：提供至少一个双链核酸分子，其中双链核酸分子的两条链在5'末端连接到固体支持物上 切割双链核酸分子的一条或两条链，并使切割的链进行变性条件以除去未连接到固体支持物上的部分切割的链，从而产生部分或基本上 用于核酸测序反应的单链模板。
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公开(公告)号：US20090118128A1
公开(公告)日：2009-05-07
申请号：US11989169
申请日：2006-07-20
申请人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
发明人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
CPC分类号： B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
摘要： The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
摘要翻译： 本发明涉及产生用于核酸测序反应的模板的方法，其包括：提供至少一个双链核酸分子，其中双链核酸分子的两条链在5'末端连接到固体支持物上 切割双链核酸分子的一条或两条链，并使切割的链进行变性条件以除去未连接到固体支持物上的部分切割的链，从而产生部分或基本上 用于核酸测序反应的单链模板。
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公开(公告)号：US20120208194A1
公开(公告)日：2012-08-16
申请号：US13450920
申请日：2012-04-19
申请人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
发明人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Barnes , Isabelle Marie Julia Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
CPC分类号： B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
摘要： The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction,
摘要翻译： 本发明涉及产生用于核酸测序反应的模板的方法，其包括：提供至少一个双链核酸分子，其中双链核酸分子的两条链在5'末端连接到固体支持物上 切割双链核酸分子的一条或两条链，并使切割的链进行变性条件以除去未连接到固体支持物上的部分切割的链，从而产生部分或基本上 用于核酸测序反应的单链模板，
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公开(公告)号：US07741463B2
公开(公告)日：2010-06-22
申请号：US11486953
申请日：2006-07-14
CPC分类号： C12Q1/6855 , C12N15/1068 , C12N15/1096 , C12Q1/686 , C40B40/06 , Y10T436/143333 , C12Q2565/525 , C12Q2525/313
摘要： The present invention relates to a method for preparing a library of template polynucleotides and use thereof in methods of solid-phase nucleic acid amplification. More specifically, the invention relates to a method for preparing a library of template polynucleotides that have common sequences at their 5′ ends and at their 3′ ends.
摘要翻译： 本发明涉及一种制备模板多核苷酸文库的方法及其在固相核酸扩增方法中的应用。 更具体地，本发明涉及一种制备在其5'末端和3'末端具有共同序列的模板多核苷酸文库的方法。
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