公开(公告)号：US09249216B2

公开(公告)日：2016-02-02

申请号：US13475553

申请日：2012-05-18

申请人： Ester Fernandez-Salas ,  Joanne Wang ,  Patton Garay ,  Lina S. Wong ,  D. Diane Hodges ,  Kei Roger Aoki

发明人： Ester Fernandez-Salas ,  Joanne Wang ,  Patton Garay ,  Lina S. Wong ,  D. Diane Hodges ,  Kei Roger Aoki

IPC分类号： G01N33/53 ,  C07K16/12 ,  G01N33/68 ,  G01N33/566 ,  C12Q1/37 ,  G01N33/50

CPC分类号： C07K16/1282 ,  C07K2317/34 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/92 ,  C12Q1/37 ,  G01N33/5014 ,  G01N33/566 ,  G01N33/6854

摘要： The present specification discloses SNAP-25 compositions, methods of making α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, methods of detecting BoNT/A activity, and methods of detecting neutralizing α-BoNT/A antibodies.

摘要翻译： 本说明书公开了SNAP-25组合物，制备α-SNAP-25抗体的方法，所述α-SNAP-25抗体结合来自SNAP-25裂解产物的BoNT / A切割位点剪切键的P1残基处的包含羧基末端的表位， SNAP-25抗体结合来自SNAP-25切割产物的BoNT / A切割位点剪切键的P1残基处的羧基末端的表位，检测BoNT / A活性的方法和检测中和α-BoNT的方法 / A抗体。

公开(公告)号：US20120225436A1

公开(公告)日：2012-09-06

申请号：US13475553

申请日：2012-05-18

申请人： Ester Fernandez-Salas ,  Joanne Wang ,  Patton Garay ,  Lina S. Wong ,  D. Diane Hodges ,  Kei Roger Aoki

发明人： Ester Fernandez-Salas ,  Joanne Wang ,  Patton Garay ,  Lina S. Wong ,  D. Diane Hodges ,  Kei Roger Aoki

IPC分类号： G01N33/53

CPC分类号： C07K16/1282 ,  C07K2317/34 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/92 ,  C12Q1/37 ,  G01N33/5014 ,  G01N33/566 ,  G01N33/6854

摘要： The present specification discloses SNAP-25 compositions, methods of making α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, methods of detecting BoNT/A activity, and methods of detecting neutralizing α-BoNT/A antibodies.

摘要翻译： 本说明书公开了SNAP-25组合物，制备α-SNAP-25抗体的方法，所述α-SNAP-25抗体结合来自SNAP-25裂解产物的BoNT / A切割位点剪切键的P1残基处的包含羧基末端的表位， SNAP-25抗体结合来自SNAP-25切割产物的BoNT / A切割位点剪切键的P1残基处的羧基末端的表位，检测BoNT / A活性的方法和检测中和α-BoNT的方法 / A抗体。

公开(公告)号：US08198034B2

公开(公告)日：2012-06-12

申请号：US12403531

申请日：2009-03-13

申请人： Ester Fernandez-Salas ,  Joanne Wang ,  Patton Garay ,  Lina S. Wong ,  D. Dianne Hodges ,  Kei Roger Aoki

发明人： Ester Fernandez-Salas ,  Joanne Wang ,  Patton Garay ,  Lina S. Wong ,  D. Dianne Hodges ,  Kei Roger Aoki

IPC分类号： G01N33/53

CPC分类号： C07K16/1282 ,  C07K2317/34 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/92 ,  C12Q1/37 ,  G01N33/5014 ,  G01N33/566 ,  G01N33/6854

摘要： The present specification discloses SNAP-25 compositions, methods of making α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, methods of detecting BoNT/A activity, and methods of detecting neutralizing α-BoNT/A antibodies.

摘要翻译： 本说明书公开了SNAP-25组合物，制备α-SNAP-25抗体的方法，所述α-SNAP-25抗体结合来自SNAP-25裂解产物的BoNT / A切割位点剪切键的P1残基处的包含羧基末端的表位， SNAP-25抗体结合来自SNAP-25切割产物的BoNT / A切割位点剪切键的P1残基处的羧基末端的表位，检测BoNT / A活性的方法和检测中和α-BoNT的方法 / A抗体。

公开(公告)号：US20120122128A1

公开(公告)日：2012-05-17

申请号：US12403531

申请日：2009-03-13

申请人： Ester Fernandez-Salas ,  Joanne Wang ,  Patton Garay ,  Lina S. Wong ,  D. Dianne Hodges ,  Kei Roger Aoki

发明人： Ester Fernandez-Salas ,  Joanne Wang ,  Patton Garay ,  Lina S. Wong ,  D. Dianne Hodges ,  Kei Roger Aoki

IPC分类号： G01N33/566

CPC分类号： C07K16/1282 ,  C07K2317/34 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/92 ,  C12Q1/37 ,  G01N33/5014 ,  G01N33/566 ,  G01N33/6854

摘要： The present specification discloses SNAP-25 compositions, methods of making α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, methods of detecting BoNT/A activity, and methods of detecting neutralizing α-BoNT/A antibodies.

摘要翻译： 本说明书公开了SNAP-25组合物，制备α-SNAP-25抗体的方法，所述α-SNAP-25抗体结合来自SNAP-25裂解产物的BoNT / A切割位点剪切键的P1残基处的包含羧基末端的表位， SNAP-25抗体结合来自SNAP-25切割产物的BoNT / A切割位点剪切键的P1残基处的羧基末端的表位，检测BoNT / A活性的方法和检测中和α-BoNT的方法 / A抗体。

公开(公告)号：US20130040368A1

公开(公告)日：2013-02-14

申请号：US13649923

申请日：2012-10-11

申请人： Ester Fernandez-Salas ,  Joanne Wang ,  Patton E. Garay ,  Lina S. Wong ,  D. Dianne Hodges ,  Kei Roger Aoki

发明人： Ester Fernandez-Salas ,  Joanne Wang ,  Patton E. Garay ,  Lina S. Wong ,  D. Dianne Hodges ,  Kei Roger Aoki

IPC分类号： C12N9/52

摘要： The present specification discloses SNAP-25 compositions, methods of making α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, methods of detecting BoNT/A activity, and methods of detecting neutralizing α-BoNT/A antibodies.

摘要翻译： 本说明书公开了SNAP-25组合物，制备α-SNAP-25抗体的方法，所述α-SNAP-25抗体结合来自SNAP-25裂解产物的BoNT / A切割位点剪切键的P1残基处的包含羧基末端的表位， SNAP-25抗体结合来自SNAP-25切割产物的BoNT / A切割位点剪切键的P1残基处的羧基末端的表位，检测BoNT / A活性的方法和检测中和α-BoNT的方法 / A抗体。

公开(公告)号：US08618261B2

公开(公告)日：2013-12-31

申请号：US12723474

申请日：2010-03-12

申请人： Fernandez-Salas Ester ,  Joanne Wang ,  Patton Garay ,  Lina S. Wong ,  D. Dianne Hodges ,  Kei Roger Aoki

发明人： Fernandez-Salas Ester ,  Joanne Wang ,  Patton Garay ,  Lina S. Wong ,  D. Dianne Hodges ,  Kei Roger Aoki

IPC分类号： C07K16/00

CPC分类号： C07K16/1282 ,  C07K2317/34 ,  C07K2317/77 ,  C12Q1/37 ,  G01N33/56911 ,  G01N2333/33 ,  G01N2333/705 ,  G01N2333/952

摘要： The present specification discloses SNAP-25 compositions, methods of making α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, methods of detecting BoNT/A activity, and methods of detecting neutralizing α-BoNT/A antibodies.

摘要翻译： 本说明书公开了SNAP-25组合物，制备α-SNAP-25抗体的方法，所述α-SNAP-25抗体结合来自SNAP-25裂解产物的BoNT / A切割位点剪切键的P1残基处的包含羧基末端的表位， SNAP-25抗体结合包含来自SNAP-25切割产物的BoNT / A切割位点剪切键的P1残基处的羧基末端的表位，检测BoNT / A活性的方法和检测中和α-BoNT的方法 / A抗体。

公开(公告)号：US20100203559A1

公开(公告)日：2010-08-12

申请号：US12723474

申请日：2010-03-12

申请人： Fernandez-Salas Ester ,  Joanne Wang ,  Patton Garay ,  Lina S. Wong ,  D. Dianne Hodges ,  Kei Roger Aoki

发明人： Fernandez-Salas Ester ,  Joanne Wang ,  Patton Garay ,  Lina S. Wong ,  D. Dianne Hodges ,  Kei Roger Aoki

IPC分类号： G01N33/53 ,  C07K7/08 ,  C07K16/18 ,  C07K7/06

CPC分类号： C07K16/1282 ,  C07K2317/34 ,  C07K2317/77 ,  C12Q1/37 ,  G01N33/56911 ,  G01N2333/33 ,  G01N2333/705 ,  G01N2333/952

摘要： The present specification discloses SNAP-25 compositions, methods of making α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, methods of detecting BoNT/A activity, and methods of detecting neutralizing α-BoNT/A antibodies.

摘要翻译： 本说明书公开了SNAP-25组合物，制备α-SNAP-25抗体的方法，所述α-SNAP-25抗体结合来自SNAP-25裂解产物的BoNT / A切割位点剪切键的P1残基处的包含羧基末端的表位， SNAP-25抗体结合来自SNAP-25切割产物的BoNT / A切割位点剪切键的P1残基处的羧基末端的表位，检测BoNT / A活性的方法和检测中和α-BoNT的方法 / A抗体。

公开(公告)号：US08455203B2

公开(公告)日：2013-06-04

申请号：US12723595

申请日：2010-03-12

申请人： Joanne Wang ,  Hong Zhu ,  Dianne D. Hodges ,  Ester Fernandez-Salas

发明人： Joanne Wang ,  Hong Zhu ,  Dianne D. Hodges ,  Ester Fernandez-Salas

IPC分类号： G01N33/53

CPC分类号： G01N33/573 ,  C07K16/18 ,  C07K2317/34 ,  C07K2317/56 ,  C07K2317/92 ,  C12N9/50 ,  C12Q1/37 ,  C12Y304/24069 ,  G01N33/5014 ,  G01N33/5035 ,  G01N33/53 ,  G01N2333/33 ,  G01N2333/47 ,  G01N2333/952 ,  G01N2333/96425

摘要： The present specification discloses SNAP-25 immune response inducing compositions, methods of making α-SNAP-25 antibodies that selectively binds to an epitope comprising a SNAP-25 having a carboxyl-terminus at the P1 residue of the BoNT/A cleavage site scissile bond, α-SNAP-25 antibodies that selectively bind to an epitope comprising a SNAP-25 having a carboxyl-terminus at the P1 residue of the BoNT/A cleavage site scissile bond, methods of detecting retargeted endopeptidase activity, and methods of detecting neutralizing α-re-targeted endopeptidase antibodies.

摘要翻译： 本说明书公开了SNAP-25免疫应答诱导组合物，制备选择性结合包含在BoNT / A切割位点剪切键的P1残基处具有羧基末端的SNAP-25的表位的α-SNAP-25抗体的方法 选择性结合包含在BoNT / A切割位点剪切键的P1残基处具有羧基末端的SNAP-25的表位的α-SNAP-25抗体，检测重靶向内肽酶活性的方法和检测中和α的方法 靶向内肽酶抗体。

公开(公告)号：US08361789B2

公开(公告)日：2013-01-29

申请号：US12722801

申请日：2010-03-12

申请人： Hong Zhu ,  Joanne Wang ,  Birgitte P. S. Jacky ,  D. Dianne Hodges ,  Ester Fernandez-Salas

发明人： Hong Zhu ,  Joanne Wang ,  Birgitte P. S. Jacky ,  D. Dianne Hodges ,  Ester Fernandez-Salas

IPC分类号： C12N15/85

CPC分类号： C12N5/0618 ,  C12N5/0693 ,  G01N33/5014

摘要： The present specification discloses clonal cell lines susceptible to BoNT/A intoxication, methods of producing such clonal cell lines, and methods of detecting Botulinum toxin serotype A activity using such clonal cell lines.

公开(公告)号：US20100233741A1

公开(公告)日：2010-09-16

申请号：US12723595

申请日：2010-03-12

申请人： Joanne Wang ,  Hong Zhu ,  Dianne D. Hodges ,  Ester Fernandez-Salas

发明人： Joanne Wang ,  Hong Zhu ,  Dianne D. Hodges ,  Ester Fernandez-Salas

IPC分类号： G01N33/53

CPC分类号： G01N33/573 ,  C07K16/18 ,  C07K2317/34 ,  C07K2317/56 ,  C07K2317/92 ,  C12N9/50 ,  C12Q1/37 ,  C12Y304/24069 ,  G01N33/5014 ,  G01N33/5035 ,  G01N33/53 ,  G01N2333/33 ,  G01N2333/47 ,  G01N2333/952 ,  G01N2333/96425

摘要： The present specification discloses SNAP-25 immune response inducing compositions, methods of making α-SNAP-25 antibodies that selectively binds to an epitope comprising a SNAP-25 having a carboxyl-terminus at the P1 residue of the BoNT/A cleavage site scissile bond, α-SNAP-25 antibodies that selectively bind to an epitope comprising a SNAP-25 having a carboxyl-terminus at the P1 residue of the BoNT/A cleavage site scissile bond, methods of detecting retargeted endopeptidase activity, and methods of detecting neutralizing α-re-targeted endopeptidase antibodies.

摘要翻译： 本说明书公开了SNAP-25免疫应答诱导组合物，制备选择性结合包含在BoNT / A切割位点剪切键的P1残基处具有羧基末端的SNAP-25的表位的α-SNAP-25抗体的方法 ，选择性结合包含在BoNT / A切割位点剪切键的P1残基处具有羧基末端的SNAP-25的表位的α-SNAP-25抗体，检测重靶向内肽酶活性的方法和检测中和α的方法 靶向内肽酶抗体。