公开(公告)号：US20230201370A1

公开(公告)日：2023-06-29

申请号：US18057359

申请日：2022-11-21

申请人： Evox Therapeutics Ltd. ,  Oxford University Innovation Limited

发明人： Justin HEAN ,  Imre MAGER ,  Matthew WOOD ,  Samir EL ANDALOUSSI ,  Oscar WIKLANDER ,  Joel NORDIN

IPC分类号： A61K48/00 ,  A61K47/65 ,  A61K41/00 ,  A61K47/64 ,  A61K47/00 ,  A61K9/127

CPC分类号： A61K48/0008 ,  A61K47/65 ,  A61K41/0042 ,  A61K47/6425 ,  A61K47/00 ,  A61K9/127 ,  A61K48/005 ,  A61K48/0091

摘要： The present invention pertains to an inventive release mechanism for extracellular vesicle (EV)-mediated intracellular and intramembrane delivery of therapeutic polypeptides. More specifically, the invention relates to EVs comprising polypeptide constructs which comprise a therapeutic polypeptide releasably attached to an exosomal polypeptide. Furthermore, the present invention pertains to manufacturing methods, pharmaceutical compositions, medical uses and applications, and various other embodiments related to the inventive EVs.

公开(公告)号：US20210062166A1

公开(公告)日：2021-03-04

申请号：US16960351

申请日：2019-01-18

申请人： Evox Therapeutics Ltd

发明人： Joel NORDIN ,  Justin HEAN ,  Per LUNDIN

IPC分类号： C12N9/10 ,  C07K16/18 ,  C07K14/705 ,  A61K9/10 ,  A61K38/45 ,  A61K39/395

摘要： The present invention relates to extracellular vesicle (EV)-mediated delivery of protein-based therapeutics. More specifically, the invention relates to delivery of complex polypeptide-based agents which typically bind to target proteins extracellularly, intracellularly, or in the cell membrane.

公开(公告)号：US20190167810A1

公开(公告)日：2019-06-06

申请号：US16304580

申请日：2017-05-25

申请人： EVOX THERAPEUTICS LTD ,  OXFORD UNIVERSITY INNOVATION

发明人： Justin HEAN ,  Imre MAGER ,  Matthew WOOD ,  Samir EL ANDALOUSSI ,  Oscar WIKLANDER ,  Joel NORDIN

IPC分类号： A61K48/00 ,  A61K9/127

摘要： The present invention pertains to an inventive release mechanism for extracellular vesicle (EV)-mediated intracellular and intramembrane delivery of therapeutic polypeptides. More specifically, the invention relates to EVs comprising polypeptide constructs which comprise a therapeutic polypeptide releasably attached to an exosomal polypeptide. Furthermore, the present invention pertains to manufacturing methods, pharmaceutical compositions, medical uses and applications, and various other embodiments related to the inventive EVs.

公开(公告)号：US20210369865A1

公开(公告)日：2021-12-02

申请号：US16762606

申请日：2018-11-08

申请人： Evox Therapeutics Ltd

发明人： Lorenzo ERRICHELLI ,  Dhanu GUPTA ,  Justin HEAN ,  Joel NORDIN

IPC分类号： A61K48/00 ,  A61K31/7088 ,  C12N9/22 ,  C12N15/88 ,  A61K9/127

摘要： The present invention pertains to extracellular vesicle (EV) therapeutics, wherein the EVs comprise nucleic acid (NA)-based therapeutics such as mRNAs, circular RNAs, miRNAs, shRNAs, circular RNA and/or DNA molecules. The NA therapeutics are loaded into EVs using inventive engineering protein and NA engineering strategies to enhance loading into EVs and to facilitate release of the NA cargo molecules inside target cells.

公开(公告)号：US20230355805A1

公开(公告)日：2023-11-09

申请号：US18028576

申请日：2021-09-29

申请人： Evox Therapeutics Ltd.

发明人： Dhanu GUPTA ,  Samir EL ANDALOUSSI ,  Oscar WIKLANDER ,  Joel NORDIN

IPC分类号： A61K48/00 ,  A61K47/69 ,  A61K9/50 ,  C07K14/47 ,  C07K14/54 ,  C07K14/705 ,  C07K14/715 ,  C12N15/88

CPC分类号： A61K48/0041 ,  A61K47/6901 ,  A61K48/0033 ,  A61K9/5068 ,  C07K14/4702 ,  C07K14/5412 ,  C07K14/70582 ,  C07K14/70596 ,  C07K14/7151 ,  C12N15/88

摘要： The present invention relates to engineered extracellular vesicles (EVs) as a therapeutic modality for the treatment of various severe diseases. More specifically, the invention relates to a novel approach to manufacturing engineered EVs which extends their half-life and alters their biodistribution, resulting in a novel therapeutic modality able to carry various types of drugs suitable for application in not only genetic diseases but more broadly across essentially all therapeutic areas.

公开(公告)号：US20200062813A1

公开(公告)日：2020-02-27

申请号：US16487667

申请日：2018-01-19

申请人： Evox Therapeutics Ltd

发明人： Joel NORDIN ,  Dhanu GUPTA

IPC分类号： C07K14/47

摘要： The present invention pertains to improved methods for loading of extracellular vesicles (EVs), such as exosomes, with various types of proteins of interest. More specifically, the invention relates to loading of EVs using fusion polypeptide constructs, as well as i.a. fusion constructs per se and EVs carrying such fusion polypeptides. The design of the fusion polypeptides is key to enable both efficient surface-display and internal loading into EVs of proteins of interest.

公开(公告)号：US20200207833A1

公开(公告)日：2020-07-02

申请号：US16795429

申请日：2020-02-19

申请人： Evox Therapeutics Ltd.

发明人： Samir EL ANDALOUSSI ,  Oscar WIKLANDER ,  Joel NORDIN ,  Edvard SMITH ,  Karl-Henrik GRINNEMO ,  Oscar SIMONSON

IPC分类号： C07K14/705 ,  A61K47/62 ,  A61K47/69 ,  F01C1/344 ,  F01C21/10

摘要： The present invention pertains to inter alia therapeutic delivery vesicles, for instance exosomes or microvesicles, comprising polypeptide constructs, methods for producing said therapeutic delivery vesicles, pharmaceutical compositions and medical uses thereof. The therapeutic polypeptide constructs comprised in the extracellular delivery vesicles enable sequestering target molecules of interest, to treat e.g. neuro-inflammatory diseases and cancer.