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公开(公告)号：US11298319B2
公开(公告)日：2022-04-12
申请号：US16317455
申请日：2017-07-10
发明人： Oscar Wiklander , Per Lundin , Dhanu Gupta
摘要： The present invention relates to methods for loading extracellular vesicles (EVs) with a pharmacological agent. The invention discloses the use of cell-penetrating peptides as carriers into EVs, using either a non-covalent or covalent loading approach. Furthermore, the present invention pertains to medical uses and compositions comprising such pharmacological agent-loaded EVs.
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公开(公告)号：US20210252164A1
公开(公告)日：2021-08-19
申请号：US17252335
申请日：2019-06-21
发明人： Samir EL ANDALOUSSI , Per LUNDIN
摘要： The present invention relates to administration of gene therapy, in particular the development of effective combinatorial strategies for gene delivery in inter alia monogenetic diseases. More specifically, the present invention relates to a combination therapy, methods of producing the combination therapy, as well as various related embodiments.
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公开(公告)号：US20210069254A1
公开(公告)日：2021-03-11
申请号：US16963883
申请日：2019-02-11
摘要： The present invention relates to compositions and buffers for storing engineered extracellular vesicles (EVs), such as exosomes, for extended time periods with maintained stability and function. The present invention allows for both optimized long-term storage and for clinical application of the stored EVs.
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公开(公告)号：US20190167810A1
公开(公告)日：2019-06-06
申请号：US16304580
申请日：2017-05-25
发明人： Justin HEAN , Imre MAGER , Matthew WOOD , Samir EL ANDALOUSSI , Oscar WIKLANDER , Joel NORDIN
摘要： The present invention pertains to an inventive release mechanism for extracellular vesicle (EV)-mediated intracellular and intramembrane delivery of therapeutic polypeptides. More specifically, the invention relates to EVs comprising polypeptide constructs which comprise a therapeutic polypeptide releasably attached to an exosomal polypeptide. Furthermore, the present invention pertains to manufacturing methods, pharmaceutical compositions, medical uses and applications, and various other embodiments related to the inventive EVs.
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公开(公告)号：US20230201370A1
公开(公告)日：2023-06-29
申请号：US18057359
申请日：2022-11-21
发明人： Justin HEAN , Imre MAGER , Matthew WOOD , Samir EL ANDALOUSSI , Oscar WIKLANDER , Joel NORDIN
CPC分类号： A61K48/0008 , A61K47/65 , A61K41/0042 , A61K47/6425 , A61K47/00 , A61K9/127 , A61K48/005 , A61K48/0091
摘要： The present invention pertains to an inventive release mechanism for extracellular vesicle (EV)-mediated intracellular and intramembrane delivery of therapeutic polypeptides. More specifically, the invention relates to EVs comprising polypeptide constructs which comprise a therapeutic polypeptide releasably attached to an exosomal polypeptide. Furthermore, the present invention pertains to manufacturing methods, pharmaceutical compositions, medical uses and applications, and various other embodiments related to the inventive EVs.
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公开(公告)号：US20230181757A1
公开(公告)日：2023-06-15
申请号：US16319087
申请日：2017-07-21
发明人： André GÖRGENS , Dhanu GUPTA , Oscar WIKLANDER
IPC分类号： A61K47/69 , G01N33/554 , G01N33/532 , C07K16/32
CPC分类号： A61K47/6901 , G01N33/554 , G01N33/532 , C07K16/32
摘要： The present invention pertains to engineered extracellular vesicles (EVs) for use in a variety of research, diagnostic, imaging and therapeutic applications. The EVs are engineered to enable incorporation and surface display of various proteins of interest, which may be exogenous and/or endogenous in nature. The coating of EVs is achieved through inventive protein engineering of EV polypeptides, and the present invention thus relates to methods for coating of EVs, EVs per se, as well kits, detection methods, diagnostic applications, imaging and delivery methods based on said engineered EVs.
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公开(公告)号：US20220202714A1
公开(公告)日：2022-06-30
申请号：US17689282
申请日：2022-03-08
发明人： Oscar WIKLANDER , Per LUNDIN , Dhanu GUPTA
摘要： The present invention relates to methods for loading extracellular vesicles (EVs) with a pharmacological agent. The invention discloses the use of cell-penetrating peptides as carriers into EVs, using either a non-covalent or covalent loading approach. Furthermore, the present invention pertains to medical uses and compositions comprising such pharmacological agent-loaded EVs.
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公开(公告)号：US20210369865A1
公开(公告)日：2021-12-02
申请号：US16762606
申请日：2018-11-08
发明人： Lorenzo ERRICHELLI , Dhanu GUPTA , Justin HEAN , Joel NORDIN
IPC分类号： A61K48/00 , A61K31/7088 , C12N9/22 , C12N15/88 , A61K9/127
摘要： The present invention pertains to extracellular vesicle (EV) therapeutics, wherein the EVs comprise nucleic acid (NA)-based therapeutics such as mRNAs, circular RNAs, miRNAs, shRNAs, circular RNA and/or DNA molecules. The NA therapeutics are loaded into EVs using inventive engineering protein and NA engineering strategies to enhance loading into EVs and to facilitate release of the NA cargo molecules inside target cells.
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公开(公告)号：US20230355805A1
公开(公告)日：2023-11-09
申请号：US18028576
申请日：2021-09-29
发明人： Dhanu GUPTA , Samir EL ANDALOUSSI , Oscar WIKLANDER , Joel NORDIN
IPC分类号： A61K48/00 , A61K47/69 , A61K9/50 , C07K14/47 , C07K14/54 , C07K14/705 , C07K14/715 , C12N15/88
CPC分类号： A61K48/0041 , A61K47/6901 , A61K48/0033 , A61K9/5068 , C07K14/4702 , C07K14/5412 , C07K14/70582 , C07K14/70596 , C07K14/7151 , C12N15/88
摘要： The present invention relates to engineered extracellular vesicles (EVs) as a therapeutic modality for the treatment of various severe diseases. More specifically, the invention relates to a novel approach to manufacturing engineered EVs which extends their half-life and alters their biodistribution, resulting in a novel therapeutic modality able to carry various types of drugs suitable for application in not only genetic diseases but more broadly across essentially all therapeutic areas.
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公开(公告)号：US20230111666A1
公开(公告)日：2023-04-13
申请号：US18064382
申请日：2022-12-12
发明人： Samir EL ANDALOUSSI , Oscar Wiklander , Joel Nordin , Edvard Smith , Karl-Henrik Grinnemo , Oscar Simonson
摘要： The present invention pertains to inter alia methods for purifying extracellular vesicles including exposing a sample comprising at least one extracellular vesicle to ultrafiltration; and exposing the sample following the ultrafiltration in step (i) to size exclusion liquid chromatography.
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