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1.发明授权公开(公告)号：US12037632B2
公开(公告)日：2024-07-16
申请号：US17263562
申请日：2019-07-13
发明人： Bin Yao , Xiaoyun Su , Fei Gao , Huiying Luo , Huoqing Huang , Yingguo Bai , Yuan Wang , Tao Tu , Yaru Wang , Kun Meng
CPC分类号： C12Q1/40 , C07K14/38 , C07K14/43504 , C12N15/80 , G01N15/1468 , C07K2319/035 , C07K2319/60 , C12R2001/885 , G01N2015/1472 , G01N2333/93
摘要： The present invention relates to the field of genetic engineering, particularly to a recombinant expression vector for rapidly screening the high expression strains and a method for rapidly screening high expression strains. In the invention, an exogenous red fluorescent protein and Aspergillus fumigatus cell surface protein localization signal are fused and expressed, and the fusion gene (DsRed-AfMP1) is integrated into the genome of Trichoderma reesei, so as to construct a strain displaying red fuorescent protein on the surface of Trichoderma reesei. By sorting Trichoderma reesei strains with red fluorescent protein on the surface by flow cytometry, genes beneficial to the improvement of cellulase activity can be quickly isolated.
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公开(公告)号：US10767170B2
公开(公告)日：2020-09-08
申请号：US15534491
申请日：2014-12-09
发明人： Bin Yao , Pengjun Shi , Hong Yang , Huoqing Huang , Huiying Luo , Peilong Yang , Yaru Wang , Xiaoyun Su , Yingguo Bai , Xia Shi , Rui Ma , Kun Meng
摘要： Provided are a fungus-sourced high-temperature neutral Family-45 cullulase as well as a coding gene and application thereof. The cullulase has optimal pH value of 5.5, and optimal temperature of 60° C., has certain enzyme activity in alkaline condition, and has good alkali resistance, maintains about 70% enzyme activity in optimal condition after being processed at 90° C. for 1 hour, maintains about 50% enzyme activity in optimal condition after being processed in boiling water for 1 hour, and can be well applied in c and other fields.
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公开(公告)号：US11634694B2
公开(公告)日：2023-04-25
申请号：US16618375
申请日：2017-05-31
发明人： Bin Yao , Xiaoyun Su , Huang Qin , Huiying Luo , Huoqing Huang , Yingguo Bai , Yuan Wang , Yaru Wang , Rui Ma , Tao Tu , Jianshuang Ma
摘要： The present invention provides use of a manganese peroxidase in the detoxification of mycotoxins, and specifically, the present invention provides five manganese peroxidases (MnP-1, MnP-2, MnP-4, MnP-5, and MnP-6), genes thereof, and uses thereof. The present invention provides five manganese peroxidases (MnP-1, MnP-2, MnP-4, MnP-5, and MnP-6) derived from lignocellulose degradation bacteria, the amino acid sequences thereof being as set forth in SEQ ID NO: 1, SEQ ID NO: 4, SEQ ID NO: 7, SEQ ID NO: 10, and SEQ ID NO: 13.
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公开(公告)号：US10920200B2
公开(公告)日：2021-02-16
申请号：US16608833
申请日：2018-06-07
发明人： Bin Yao , Huiying Luo , Weina Liu , Jianzhong Ge , Tao Tu , Huoqing Huang , Xiaoyun Su , Yingguo Bai , Yuan Wang , Yaru Wang , Kun Meng
摘要： Provided are a glucose oxidase CnGODA, an encoding gene thereof, a recombinant expression vector comprising the gene, and a recombinant strain; the amino acid sequence of the glucose oxidase CnGODA is as represented in SEQ ID NO:1 or SEQ ID NO:2. Further provided is a method for use in preparing glucose oxidase CnGODA, and application of glucose oxidase CnGODA.
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5.发明授权公开(公告)号：US10415025B2
公开(公告)日：2019-09-17
申请号：US15534006
申请日：2014-12-08
发明人： Bin Yao , Pengjun Shi , Wei Xia , Huiying Luo , Huoqing Huang , Peilong Yang , Yaru Wang , Xiaoyun Su , Yingguo Bai , Xia Shi , Rui Ma
IPC分类号： C12N9/42 , C12N15/00 , C12N1/20 , C07H21/04 , C12P21/06 , C12P21/04 , C12N1/14 , A23K20/189 , A23L33/10 , A23L29/00 , A23K10/14
摘要： Provided are a fungus-sourced high-temperature acid β-glucosidase as well as a coding gene, and an application thereof. The provide β-glucosidase has the optimal pH value of 4.5 and the optimal temperature of 75° C., and maintains over 90% enzyme activity in the optimal condition after being processed at 60° C. for 1 h. The re-engineering yeast strain GS115/bgl3A of the coding gene comprising the β-glucosidase has high fermentation level.
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公开(公告)号：US11407987B2
公开(公告)日：2022-08-09
申请号：US17054526
申请日：2019-05-10
发明人： Huiying Luo , Bin Yao , Weina Liu , Yuan Gu , Tao Tu , Yuan Wang , Yaru Wang , Huoqing Huang , Yingguo Bai , Xiaoyun Su , Kun Meng
摘要： The present invention relates to a mannanase PMan5A mutant having improved heat resistance, a gene encoding the mutant and application thereof. The mutant is obtained by a substitution of histidine with tyrosine at amino acid residue 93, phenylalanine with tyrosine at amino acid residue 94, leucine with histidine at amino acid residue 356, and/or alanine with proline at amino acid residue 389. The thermal tolerance of the single site mutation mutant H93Y, L356H and A389P are greatly improved over that of the wild-type mannanase PMan5A, and the thermal tolerance of the combination mutants shows the stack effect of the single site mutation, demonstrating the amino acids at the sites of 93, 94, 356, and 389 play an important role for the thermal stability of the mannanase of GH5 family.
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7.发明授权公开(公告)号：US11028377B2
公开(公告)日：2021-06-08
申请号：US16477907
申请日：2018-01-15
发明人： Canfang Niu , Peilong Yang , Bin Yao , Yangyang Li , Dali Yu , Huiying Luo , Huoqing Huang , Yaru Wang
摘要： The present invention relates to the field of genetic engineering, particularly to phytase variants YkAPPA having amino acid sequence substituting Leucine at the 162th site of the sequence set forth in SEQ ID NO.1 with glycine or proline, or having amino acid sequence substituting glutamic acid at the 230th site of the sequence set forth in SEQ ID NO.1 with glycine, alanine, serine, threonine, aspartic acid, proline, or arginine, and having improved pepsin resistance and increased catalytic efficiency of 2.1 times of that of the wild phytase, in the benefit of the development of economical feed enzyme industry.
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公开(公告)号：US10920206B2
公开(公告)日：2021-02-16
申请号：US16344699
申请日：2016-12-15
发明人： Bin Yao , Huiying Luo , Yeqing Li , Yuan Wang , Tao Tu , Huoqing Huang , Yingguo Bai , Yaru Wang , Xiaoyun Su
摘要： Provided are acidic thermophilic polygalacturonase TePG28A, encoding gene and application thereof. The amino acid sequence thereof is as shown in SEQ ID NO. 1 or SEQ ID NO. 2. The expressed acidic thermophilic polygalacturonase by means of cloning has advantages such as high enzyme activity and high stability; can adapt to the high temperature environment in the industrial production; has better application prospect; and can effectively degrade pectic substances such as polygalacturonic acid and pectin; and can be effectively applied to the industrial field of feed, food, and textile, etc.
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公开(公告)号：US11680255B2
公开(公告)日：2023-06-20
申请号：US17283565
申请日：2019-09-30
发明人： Huiying Luo , Bin Yao , Yujie Guo , Tao Tu , Yuan Wang , Huoqing Huang , Yingguo Bai , Xiaoyun Su , Yaru Wang , Kun Meng
IPC分类号： C12N9/34
CPC分类号： C12N9/2428 , C12Y302/01003
摘要： The present invention relates to the field of genetic engineering, particularly to a glucoamylase TIGa15, gene and application thereof. Said glucoamylase comprises the amino acid sequence of SEQ ID NO:1 or SEQ ID NO:2 and has the excellent enzymic properties, which can be applied to feed, food, and medicine industries, can be industrially produce with the genetic engineering technics.
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