公开(公告)号：US20190112565A1

公开(公告)日：2019-04-18

申请号：US16219872

申请日：2018-12-13

Applicant: FUJIFILM CORPORATION

Inventor： Hideaki KAGAWA ,  Yoichi NAGAI ,  Shinichi NAKAI ,  Souichi KOHASHI ,  Toshiki TAKEI

IPC: C12M3/02 ,  C12N5/078 ,  B01D29/01 ,  B01D39/10 ,  B01D39/08 ,  B01D39/14

Abstract: The present disclosure provides a membrane separation method of a cell suspension which can appropriately separate cells from debris, and a cell culture device. That is, membrane separation processing of the cell suspension is performed using a filtration membrane which includes an inlet-side opening formed on one surface and an outlet-side opening, which is formed on the other surface and communicates with the inlet-side opening, and in which the inlet-side opening and the outlet-side opening are disposed at positions deviated in a direction parallel to the surfaces of the membrane.

公开(公告)号：US20190322975A1

公开(公告)日：2019-10-24

申请号：US16460983

申请日：2019-07-02

Applicant: FUJIFILM CORPORATION

Inventor： Shinichi NAKAI ,  Yoichi NAGAI ,  Nobuyuki HARAGUCHI ,  Shigehisa SUGIYAMA

IPC: C12M1/00 ,  B01D61/14 ,  B01D61/18 ,  C12M1/26 ,  C12N5/00 ,  C12M1/12

Abstract: Provided is a cell culture apparatus including a culture vessel that stores a cell suspension containing cells; a first filter part that has a first filter membrane that performs membrane separation treatment on the cell suspension extracted from the culture vessel; a first circulation flow path that allows components blocked by the first filter membrane to return to the culture vessel; a second filter part that has a second filter membrane that performs membrane separation treatment on components of the cell suspension permeated through the first filter membrane; a second circulation flow path that allows components permeated through the second filter membrane to return to the culture vessel; and a recovery flow path that recovers components blocked by the second filter membrane. In the cell culture apparatus, an average hole diameter of the first filter membrane is 20 μm or smaller, and 0

公开(公告)号：US20230332085A1

公开(公告)日：2023-10-19

申请号：US18337146

申请日：2023-06-19

Applicant: FUJIFILM CORPORATION

Inventor： Shinichi NAKAI ,  Yoichi NAGAI ,  Nobuyuki HARAGUCHI ,  Shigehisa SUGIYAMA

IPC: C12M1/00 ,  B01D61/58 ,  C12M1/12 ,  B01D61/14 ,  C12M1/26 ,  C12N5/00 ,  B01D61/18

CPC classification number: C12M29/04 ,  B01D61/145 ,  B01D61/146 ,  B01D61/147 ,  B01D61/18 ,  B01D61/58 ,  C12M29/10 ,  C12M29/18 ,  C12M33/14 ,  C12M37/02 ,  C12M39/00 ,  C12M47/02 ,  C12N5/0081 ,  B01D2311/25 ,  B01D2311/2688 ,  B01D2315/08 ,  B01D2315/10 ,  B01D2317/025

Abstract: Provided is a cell culture apparatus including a culture vessel that stores a cell suspension containing cells; a first filter part that has a first filter membrane that performs membrane separation treatment on the cell suspension extracted from the culture vessel; a first circulation flow path that allows components blocked by the first filter membrane to return to the culture vessel; a second filter part that has a second filter membrane that performs membrane separation treatment on components of the cell suspension permeated through the first filter membrane; a second circulation flow path that allows components permeated through the second filter membrane to return to the culture vessel; and a recovery flow path that recovers components blocked by the second filter membrane. In the cell culture apparatus, an average hole diameter of the first filter membrane is 20 µm or smaller, and 0

公开(公告)号：US20220127593A1

公开(公告)日：2022-04-28

申请号：US17572011

申请日：2022-01-10

Applicant: FUJIFILM Corporation

Inventor： Yoichi NAGAI ,  Naoto TAKAHASHI ,  Michiko EDO

IPC: C12N13/00 ,  C12N15/85

Abstract: The producing method according to the disclosed technology is a producing method for an organism-derived material into which a bioactive substance has been introduced, the producing method comprising a step of causing a suspension containing the organism-derived material before the introduction of the bioactive substance and containing the bioactive substance to pass through a first electric field region having a first electric field intensity; and a step of causing the suspension to pass through a second electric field region having a second electric field intensity lower than the first electric field intensity after the suspension has passed through the first electric field region. The organism-derived material is a human-derived cell.