公开(公告)号：US20190078058A1

公开(公告)日：2019-03-14

申请号：US16180005

申请日：2018-11-05

Applicant: FUJIFILM CORPORATION ,  Kyoto University

Inventor： Hideaki KAGAWA ,  Shun GOTO ,  Souichi KOHASHI ,  Norio NAKATSUJI ,  Kazuhiro AIBA

IPC: C12N5/074

Abstract: The present disclosure provides a method for subculturing pluripotent stem cells suitable for mass culture. The method for subculturing pluripotent stem cells includes: a culture step of culturing pluripotent stem cells to obtain a cell aggregation; and a dividing step of dividing the cell aggregation by passing the cell aggregation through a mesh-like film, which has a plurality of through-holes each having an opening dimension of 30 μm to 80 μm, at a speed of 15 cm/sec to 150 cm/sec.

公开(公告)号：US20190112565A1

公开(公告)日：2019-04-18

申请号：US16219872

申请日：2018-12-13

Applicant: FUJIFILM CORPORATION

Inventor： Hideaki KAGAWA ,  Yoichi NAGAI ,  Shinichi NAKAI ,  Souichi KOHASHI ,  Toshiki TAKEI

IPC: C12M3/02 ,  C12N5/078 ,  B01D29/01 ,  B01D39/10 ,  B01D39/08 ,  B01D39/14

Abstract: The present disclosure provides a membrane separation method of a cell suspension which can appropriately separate cells from debris, and a cell culture device. That is, membrane separation processing of the cell suspension is performed using a filtration membrane which includes an inlet-side opening formed on one surface and an outlet-side opening, which is formed on the other surface and communicates with the inlet-side opening, and in which the inlet-side opening and the outlet-side opening are disposed at positions deviated in a direction parallel to the surfaces of the membrane.

公开(公告)号：US20190201585A1

公开(公告)日：2019-07-04

申请号：US16294931

申请日：2019-03-07

Applicant: FUJIFILM CORPORATION

Inventor： Koju ITO ,  Shun GOTO ,  Souichi KOHASHI ,  Kohji NAKAZAWA ,  Hiroshi YABU

IPC: A61L27/56 ,  C12N5/0775 ,  C12N5/077 ,  C12N5/071 ,  C12N5/078

CPC classification number: A61L27/56 ,  C08J9/28 ,  C12M1/00 ,  C12M3/00 ,  C12N5/0651 ,  C12N5/0656 ,  C12N5/0663 ,  C12N5/069

Abstract: Provided are a method for producing a cell tissue, including a culturing step of culturing cells capable of serving as a feeder inside opening pores and communicating pores of a porous film having a plurality of the opening pores provided on a surface thereof and the communicating pores communicating mutually adjacent opening pores with one another; and a porous film including a plurality of opening pores provided on a surface thereof and communicating pores communicating mutually adjacent opening pores with one another.

公开(公告)号：US20180257336A1

公开(公告)日：2018-09-13

申请号：US15977773

申请日：2018-05-11

Applicant: FUJIFILM Corporation

Inventor： Koju ITO ,  Souichi KOHASHI ,  Hiroshi YABU ,  Kuniaki NAGAMINE ,  Matsuhiko NISHIZAWA

IPC: B32B7/02 ,  C08J9/26 ,  C08J9/36 ,  H01B5/14 ,  H05K9/00 ,  B32B3/12

CPC classification number: B32B7/02 ,  B32B3/12 ,  C08J9/26 ,  C08J9/28 ,  C08J9/36 ,  C08J9/365 ,  C08J2201/0502 ,  C08J2309/00 ,  C08J2383/04 ,  C08J2400/26 ,  C08J2433/26 ,  H01B5/14 ,  H05K9/00 ,  Y10T428/24967

Abstract: There is provided a conductive film having conductivity in a surface direction, being deformable, having excellent durability, and transmitting visible light.A conductive film includes a film substrate and a conductive material layer. The conductive material layer is provided on a first substrate surface of the film substrate. A plurality of through holes penetrating the film substrate and the conductive material layer in a thickness direction are formed in the conductive film. The conductive material layer has a plurality of conductive portions, and the conductive portions are present between adjacent layer opening portions. The number of the conductive portion is 400 per 1 mm2, and an opening ratio of the conductive material layer is at least 40%.

公开(公告)号：US20170306279A1

公开(公告)日：2017-10-26

申请号：US15649652

申请日：2017-07-14

Applicant: FUJIFILM CORPORATION

Inventor： Hideaki KAGAWA ,  Shun GOTO ,  Souichi KOHASHI ,  Hidekazu YAMAZAKI

IPC: C12M3/00 ,  C12M1/26 ,  C12M1/06 ,  C12M1/00

Abstract: A culture container has a first inflow port and a first outflow port. The first flow path connects the first outflow port to the first inflow port. A storage container is provided within the first flow path and has a second inflow port which is connected to the first outflow port and a second outflow port which is connected to the first inflow port. A second flow path connects a first region within the first flow path, and a second region within the first flow path. A division processing portion is provided within the second flow path, performs a division process of dividing a cell aggregation flowing in from the first flow path, and allows the cells subjected to the division process to flow out into the first flow path via the second region. A medium supply portion supplies a medium to the inside of the first flow path.

公开(公告)号：US20180216075A1

公开(公告)日：2018-08-02

申请号：US15939300

申请日：2018-03-29

Applicant: FUJIFILM Corporation

Inventor： Koju ITO ,  Shun GOTO ,  Souichi KOHASHI

IPC: C12N5/071 ,  C12M3/00 ,  C12M1/12

CPC classification number: C12N5/0671 ,  C12M21/08 ,  C12M23/34 ,  C12M25/02 ,  C12M25/14 ,  C12M33/00 ,  C12N1/04 ,  C12N5/0068 ,  C12N2533/40

Abstract: Provided is a composite binder material, a cell aggregate, a cell accumulation method, a cell preservation method, and a method of manufacturing a composite binder that can supply oxygen and nutrients to cells.A composite binder material comprises a binder material and a cell non-adhesive portion. A binder material formed in a membrane shape is provided between a first cell sheet and a second cell sheet. The cell non-adhesive portion formed at least in a portion of one membrane surface and/or other membrane surface of the binder material. A plurality of pores are formed in the binder material, and the pores open to the one membrane surface coming into contact with one sheet surface of the first cell sheet. The pores penetrate in a thickness direction and a surface direction along the one membrane surface. The porosity of the binder material is set to be at least 50%.

公开(公告)号：US20160082148A1

公开(公告)日：2016-03-24

申请号：US14851467

申请日：2015-09-11

Applicant: FUJIFILM CORPORATION

Inventor： Koju ITO ,  Souichi KOHASHI ,  Hidekazu YAMAZAKI ,  Kenichiro HATA ,  Katsura SUGAWARA ,  Eiko GOTO ,  Masatsugu SHIMOMURA ,  Hiroshi YABU ,  Takahito KAWANO

IPC: A61L27/18 ,  A61L27/56

CPC classification number: A61L31/06 ,  A61L15/425 ,  A61L15/58 ,  A61L15/64 ,  C08L67/04

Abstract: Disclosed is a film which is able to suppress agglutination by being continuously adhered to an adhesion target portion in a biological body while suppressing a position shift. A film includes an adhesive and an adhesion inhibiting layer. The adhesive layer absorbs and maintains a liquid in a plurality of pores which are opened in one film surface and has a capillary force for adhering the adhesive layer to a first-cell group. The adhesive layer is formed of a biodegradable polymer. The adhesion inhibiting layer configures the other film surface, and inhibits adhesion between a second-cell group which is different from the first-cell group and the adhesive layer. In the adhesive layer, the pore is formed not to be penetrated in a thickness direction of the film, and thus the first-cell group and the second-cell group are separated into the one film surface side and the other film surface side.

Abstract translation: 公开了一种能够通过在抑制位置偏移的同时在生物体中连续粘附到粘合目标部分来抑制凝集的膜。 膜包括粘合剂和粘附抑制层。 粘合剂层吸收并保持在一个膜表面中打开的多个孔中的液体，并且具有用于将粘合剂层粘合到第一电池组的毛细管力。 粘合剂层由可生物降解的聚合物形成。 粘合抑制层构成另一个膜表面，并且抑制不同于第一电池组的第二电池组与粘合剂层之间的粘合。 在粘合剂层中，形成不在膜的厚度方向上穿透的孔，因此第一电池组和第二电池组分离成一个膜表面侧和另一个膜表面侧。

公开(公告)号：US20160059537A1

公开(公告)日：2016-03-03

申请号：US14841174

申请日：2015-08-31

Applicant: FUJIFILM Corporation

Inventor： Koju ITO ,  Souichi KOHASHI ,  Hiroshi YABU ,  Yuta SAITO

IPC: B32B38/10 ,  B32B7/12 ,  B29C44/20 ,  B32B37/12 ,  B29C39/00 ,  B29C39/14 ,  B32B3/12 ,  B32B27/30

CPC classification number: B32B38/10 ,  B29C39/003 ,  B29C39/148 ,  B29C41/28 ,  B29C44/20 ,  B29C59/005 ,  B29K2077/00 ,  B29K2105/0073 ,  B29K2105/045 ,  B29L2007/001 ,  B29L2007/008 ,  B32B3/12 ,  B32B3/266 ,  B32B7/12 ,  B32B27/06 ,  B32B27/302 ,  B32B27/308 ,  B32B27/365 ,  B32B37/12 ,  B32B2307/73 ,  B32B2377/00 ,  B32B2457/00 ,  B32B2551/00

Abstract: Film, which has a fine structure and is adhered to various materials in an easy and strong manner without an adhesive agent, and a composite structure, film laminate, producing method and etching method, are provided. Solution in which a hydrophobic high molecular compound and a catechol group-containing compound are dissolved in solvent is cast, to form cast film. Dew is condensed on an uncovered surface of the cast film. The solvent and water droplets upon the condensation are evaporated from the cast film, to produce porous film. The porous film has a honeycomb structure in which plural pores are formed in one film surface. The pores are in a substantially equal shape and size, and are arranged regularly at a constant pitch. The film surface on a side having the pores of the porous film is a functional surface having an adhesive property.

Abstract translation: 提供具有精细结构的薄膜，并且在没有粘合剂的情况下以易于而强的方式粘附到各种材料上，并且提供复合结构，膜层压体，制造方法和蚀刻方法。 将疏水性高分子化合物和含儿茶酚基的化合物溶解在溶剂中的溶液流延形成流延膜。 在流延膜的未覆盖表面上露出凝结。 冷凝后的溶剂和水滴从流延膜蒸发，产生多孔膜。 多孔膜具有在一个膜表面形成有多个孔的蜂窝结构体。 孔的形状和尺寸基本相等，并且以恒定的间距规则地排列。 具有多孔膜的孔的一侧的膜表面是具有粘合性的功能性表面。