公开(公告)号：US20190112565A1

公开(公告)日：2019-04-18

申请号：US16219872

申请日：2018-12-13

Applicant: FUJIFILM CORPORATION

Inventor： Hideaki KAGAWA ,  Yoichi NAGAI ,  Shinichi NAKAI ,  Souichi KOHASHI ,  Toshiki TAKEI

IPC: C12M3/02 ,  C12N5/078 ,  B01D29/01 ,  B01D39/10 ,  B01D39/08 ,  B01D39/14

Abstract: The present disclosure provides a membrane separation method of a cell suspension which can appropriately separate cells from debris, and a cell culture device. That is, membrane separation processing of the cell suspension is performed using a filtration membrane which includes an inlet-side opening formed on one surface and an outlet-side opening, which is formed on the other surface and communicates with the inlet-side opening, and in which the inlet-side opening and the outlet-side opening are disposed at positions deviated in a direction parallel to the surfaces of the membrane.

公开(公告)号：US20200071651A1

公开(公告)日：2020-03-05

申请号：US16674613

申请日：2019-11-05

Applicant: FUJIFILM Corporation

Inventor： Toshiki TAKEI ,  Tadanori YAMADA ,  Ryuta TAKEGAMI ,  Kuniyuki KAMINAGA

IPC: C12M1/00 ,  B01D71/34 ,  A61K35/19 ,  A61P7/04 ,  C12N5/078 ,  B01D71/68 ,  B01D67/00

Abstract: An object of the present invention is to provide a separation substrate having a high megakaryocyte blocking rate and a high platelet permeation rate, and a cell separation filter and a method for producing a platelet which use the same. The separation substrate of the present invention is a separation substrate including a porous membrane for separating a platelet from a cell suspension containing a megakaryocyte and the platelet, in which an average pore diameter of the separation substrate is 2.0 μm to 12.0 μm, and the separation substrate is formed of at least one resin selected from the group consisting of a polysulfone resin and a polyvinylidene fluoride resin.

公开(公告)号：US20200024565A1

公开(公告)日：2020-01-23

申请号：US16570327

申请日：2019-09-13

Applicant: FUJIFILM Corporation

Inventor： Toshiki TAKEI ,  Tadanori Yamada ,  Kenichiro Hata

IPC: C12M1/26 ,  C12N5/078

Abstract: An object of the present invention is to provide a method for efficiently separating megakaryocytes and platelets produced from the megakaryocytes, and a platelet separation kit for efficiently separating megakaryocytes and platelets produced from the megakaryocytes. According to the present invention, a method for separating megakaryocytes and platelets, including an incorporation step of incorporating magnetic fine particles into at least megakaryocytes; a culture step of culturing the megakaryocytes in a culture solution to produce platelets before and/or after the incorporation step; a magnetic field application step of applying a magnetic field to the culture solution after the incorporation step and the culture step; and a recovery step of recovering the culture solution after the magnetic field application step, is provided.

公开(公告)号：US20200002675A1

公开(公告)日：2020-01-02

申请号：US16570626

申请日：2019-09-13

Applicant: FUJIFILM Corporation

Inventor： Toshiki TAKEI ,  Tadanori YAMADA

IPC: C12N5/078 ,  C12M1/00 ,  B01L3/00 ,  A61M1/02

Abstract: An object of the present invention is to provide a method for efficiently separating megakaryocytes and platelets produced from the megakaryocytes, and an instrument for efficiently separating megakaryocytes and platelets produced from the megakaryocytes. According to the present invention, a method for separating megakaryocytes and platelets, including a contact step of bringing a culture solution that contains at least megakaryocytes into contact with a substrate coated with a biocompatible polymer that adheres to the megakaryocytes via at least one of a VLA-4 integrin or a VLA-5 integrin; a culture step of culturing the megakaryocytes to produce platelets before and/or after the contact step; and a recovery step of recovering the culture solution after the contact step and the culture step is provided.

公开(公告)号：US20240402168A1

公开(公告)日：2024-12-05

申请号：US18807239

申请日：2024-08-16

Applicant: FUJIFILM Corporation

Inventor： Toshiki TAKEI ,  Koichi SAITO

IPC: G01N33/543 ,  G01N1/02 ,  G01N21/80 ,  G01N33/68

Abstract: The immunochromatographic kit includes a cartridge that accommodates an examination strip on which a specimen is spread and an examination region for capturing a test substance contained in the specimen is provided, and an accessory that is used for carrying out at least any of collection of the specimen or pretreatment carried out on the specimen before the specimen is spread on the examination strip, where a part of at least any of the examination strip or the accessory includes a portion in which a coloring reaction is exhibited by a reaction with a specimen-derived component different from the test substance in the specimen.

公开(公告)号：US20200048597A1

公开(公告)日：2020-02-13

申请号：US16659242

申请日：2019-10-21

Applicant: FUJIFILM Corporation

Inventor： Toshiki TAKEI ,  Tadanori Yamada ,  Ryuta Takegami ,  Kuniyuki Kaminaga ,  Akihito Fukunaga ,  Kazuhide Kanemura

IPC: C12M1/00 ,  B01D39/18 ,  C12N5/078

Abstract: An object of the present invention is to provide a separation substrate having a high megakaryocyte blocking rate and a high platelet permeation rate, and a cell separation filter and a method for producing a platelet which use the same. The separation substrate of the present invention is a separation substrate including non-woven fabric for separating a platelet from a cell suspension containing a megakaryocyte and the platelet, in which an average pore diameter of the separation substrate is 2.0 μm to 15.0 μm, and a thickness of the separation substrate is 10 μm to 500 μm.