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    Genetic sequences having methyltransferase activity and uses therefor
    3.
    发明授权
    Genetic sequences having methyltransferase activity and uses therefor 有权
    具有甲基转移酶活性的遗传序列及其用途

    公开(公告)号：US07807877B2

    公开(公告)日：2010-10-05

    申请号：US10502515

    申请日：2003-01-24

    申请人： Filippa Brugliera ,  Linda Demelis ,  Ronald Koes ,  Yoshikazu Tanaka

    发明人： Filippa Brugliera ,  Linda Demelis ,  Ronald Koes ,  Yoshikazu Tanaka

    IPC分类号： C12N15/29 ,  C12N15/82 ,  A01H5/00 ,  A01H5/10

    CPC分类号： C12N9/1007 ,  C12N15/825 ,  C12N15/827

    摘要： The present invention relates generally to a genetic sequence encoding a polypeptide having methyltransferase activity and the use of the genetic sequence and/or the polypeptide to modify one or more phenotypic characteristics of a plant. More particularly, the methyltransferase of the present invention acts on flavonoids, preferably wherein the flavonoid is an anthocyanin. Even more particularly, the present invention relates to a polypeptide having S-adenosyl-L-methionine:anthocyanin 3′-O-methyl-transferase or S-adenosyl-L-methionine:anthocyanin 3′,5′-O-methyltransferase activity. The present invention still further provides a genetic sequence encoding a polypeptide having methyltransferase activity derived from Petunia, Torenia Fuchsia or Plumbago or botanically related plants. The instant invention further relates to antisense and sense molecules corresponding to all or part of the subject genetic sequence as well as genetically modified plants as well as cut flowers, parts, extracts and reproductive tissue from such plants.

    摘要翻译： 本发明一般涉及编码具有甲基转移酶活性的多肽的遗传序列，以及遗传序列和/或多肽修饰植物的一种或多种表型特征的用途。 更具体地，本发明的甲基转移酶作用于黄酮类化合物，优选其中类黄酮是花青素。 更具体地，本发明涉及具有S-腺苷-L-甲硫氨酸：花青素3'-O-甲基转移酶或S-腺苷-L-甲硫氨酸：花青素3'，5'-O-甲基转移酶活性的多肽。 本发明还进一步提供了编码具有衍生自矮牵牛属，Torenia Fuchsia或Plumbago或植物相关植物的甲基转移酶活性的多肽的遗传序列。 本发明还涉及对应于全部或部分受试者遗传序列以及转基因植物以及来自这些植物的切花，部分，提取物和生殖组织的反义和有义分子。

    Genetic sequences having methyltransferase activity and uses therefor
    4.
    发明申请
    Genetic sequences having methyltransferase activity and uses therefor 有权
    具有甲基转移酶活性的遗传序列及其用途

    公开(公告)号：US20070150984A1

    公开(公告)日：2007-06-28

    申请号：US10502515

    申请日：2003-01-24

    申请人： Filippa Brugliera ,  Linda Demelis ,  Ronald Koes ,  Yoshikazu Tanaka

    发明人： Filippa Brugliera ,  Linda Demelis ,  Ronald Koes ,  Yoshikazu Tanaka

    IPC分类号： A01H1/00 ,  C07H21/04 ,  C12P21/06 ,  C12N9/10 ,  C12N5/04

    CPC分类号： C12N9/1007 ,  C12N15/825 ,  C12N15/827

    摘要： The present invention relates generally to a genetic sequence encoding a polypeptide having methyltransferase activity and the use of the genetic sequence and/or the polypeptide to modify one or more phenotypic characteristics of a plant. More particularly, the methyltransferase of the present invention acts on flavonoids, preferably wherein the flavonoid is an anthocyanin. Even more particularly, the present invention relates to a polypeptide having S-adenosyl-L-methionine:anthocyanin 3′-O-methyl-transferase or S-adenosyl-L-methionine:anthocyanin 3′,5′-O-methyltransferase activity. The present invention still further provides a genetic sequence encoding a polypeptide having methyltransferase activity derived from Petunia, Torenia Fuchsia or Plumbago or botanically related plants. The instant invention further relates to antisense and sense molecules corresponding to all or part of the subject genetic sequence as well as genetically modified plants as well as cut flowers, parts, extracts and reproductive tissue from such plants.

    摘要翻译： 本发明一般涉及编码具有甲基转移酶活性的多肽的遗传序列，以及遗传序列和/或多肽修饰植物的一种或多种表型特征的用途。 更具体地，本发明的甲基转移酶作用于黄酮类化合物，优选其中类黄酮是花青素。 更具体地，本发明涉及具有S-腺苷-L-甲硫氨酸：花青素3'-O-甲基转移酶或S-腺苷-L-甲硫氨酸：花青素3'，5'-O-甲基转移酶活性的多肽。 本发明还进一步提供了编码具有衍生自矮牵牛属，Torenia Fuchsia或Plumbago或植物相关植物的甲基转移酶活性的多肽的遗传序列。 本发明还涉及对应于全部或部分受试者遗传序列以及转基因植物以及来自这些植物的切花，部分，提取物和生殖组织的反义和有义分子。

    Plant anthocyanidin rutinoside aromatic acyl transferases
    5.
    发明授权
    Plant anthocyanidin rutinoside aromatic acyl transferases 有权
    植物花青素芸香苷酰芳香酰基转移酶

    公开(公告)号：US07173164B2

    公开(公告)日：2007-02-06

    申请号：US10259549

    申请日：2002-09-27

    申请人： Filippa Brugliera ,  Ronald Koes

    发明人： Filippa Brugliera ,  Ronald Koes

    IPC分类号： C12N15/29 ,  C12N15/52 ,  C12N15/82 ,  A01H5/00 ,  A01H5/10

    CPC分类号： C12N9/1029 ,  C12N9/1025 ,  C12N15/8243 ,  C12N15/825 ,  Y02E50/17

    摘要： The present invention relates generally to a genetic sequence encoding a polypeptide having aromatic acyl group transfer activity and the use of the genetic sequence and/or its corresponding polypeptide thereof. More particularly, the present invention provides a genetic sequence encoding a polypeptide having aromatic acyl group transfer activity derived from Petunia, Nierembergia and Viola spp. Even more particularly, the present invention relates to a genetic sequence encoding a polypeptide having aromatic acyl group transferase activity to anthocyanidin-rutinoside. The present invention also provides a genetic sequence encoding a polypeptide having aromatic acyl group transferase activity to anthocyanidin 3-O-rutinoside. The instant invention further relates to antisense and sense molecules corresponding to all or part of the subject genetic sequence as well as genetically modified plants as well as cut flowers, parts and reproductive tissue from such plants.

    摘要翻译： 本发明一般涉及编码具有芳族酰基转移活性的多肽的遗传序列和遗传序列和/或其相应多肽的用途。 更具体地说，本发明提供编码多肽的遗传序列，该多肽具有衍生自矮牵牛，纽伦堡和中华堇属的芳族酰基转移活性。 更具体地，本发明涉及编码对花色素苷 - 芸香糖苷具有芳香族酰基转移酶活性的多肽的遗传序列。 本发明还提供了编码对花色素3-O-芸香糖苷具有芳香族酰基转移酶活性的多肽的遗传序列。 本发明还涉及对应于全部或部分受试者遗传序列以及转基因植物以及来自这些植物的切花，部分和生殖组织的反义和有义分子。

    Dianthus plant named ‘Florijade’
    6.
    植物专利
    Dianthus plant named ‘Florijade’ 有权
    石竹植物名为“Florijade”

    公开(公告)号：USPP21515P2

    公开(公告)日：2010-11-23

    申请号：US12454905

    申请日：2009-05-26

    申请人： Filippa Brugliera

    发明人： Filippa Brugliera

    IPC分类号： A01H5/00

    CPC分类号： A01H5/02

    摘要： A new cultivar of Dianthus plant named ‘FLORIJADE’ is characterized, inter alia, by pronounced spray habit, perennial and profuse bloom, and green foliage. Dianthus ‘FLORIJADE’ is suitable for use, for example, as a flowering plant in pots, containers, window boxes, and the garden, but is primarily suited for the production of cut flowers. Dianthus ‘FLORIJADE’ is not hardy and is typically grown in a glasshouse. These traits set ‘FLORIJADE’ apart from all existing varieties, lines, strains, or sports of Dianthus. In particular, Dianthus ‘FLORIJADE’ has bright purple/violet flowers.

    摘要翻译： 名为“FLORIJADE”的石竹植物新品种的特征是特别是喷雾习性显着，多年生植物和多汁的植物和绿色的叶子。 石竹'FLORIJADE'适用于例如花盆，容器，窗框和花园中的开花植物，但主要适用于生产切花。 石竹'FLORIJADE'不硬，通常种植在温室中。 除了所有现有品种，线条，品种或运动的石竹之外，这些特征都设定了“佛罗伦萨”。 特别地，石竹'FLORIJADE'具有明亮的紫色/紫罗兰花。

    Flavanoid 3',5' hydroxylase gene sequences and uses therefor
    7.
    发明申请
    Flavanoid 3',5' hydroxylase gene sequences and uses therefor 有权
    类黄酮3'，5'羟化酶基因序列及其用途

    公开(公告)号：US20070033674A1

    公开(公告)日：2007-02-08

    申请号：US10526133

    申请日：2003-08-29

    申请人： Filippa Brugliera ,  Yoshikazu Tanaka ,  John Mason

    发明人： Filippa Brugliera ,  Yoshikazu Tanaka ,  John Mason

    IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N9/10 ,  C12N15/82 ,  C12N5/04

    CPC分类号： C12N9/0073 ,  C12N15/825

    摘要： The present invention relates generally to a genetic sequence encoding a polypeptide having flavonoid 3′,5′-hydroxylase (F3′5′H) activity and to the use of the genetic sequence and/or its corresponding polypeptide thereof inter alia to manipulate color in flowers or parts thereof or in other plant tissue. More particularly, the F3′5′H has the ability to modulate dihydrokaempferol (DHK) metabolism as well as the metabolism of other substrates such as dihydroquercetin (DHQ), naringenin and eriodictyol. Even more particularly, the present invention provides a genetic sequence encoding a polypeptide having F3′5′H activity when expressed in rose or gerbera or botanically related plants. The instant invention further relates to antisense and sense molecules or RNAi-inducing molecules corresponding to all or part of the subject genetic sequence or a transcript thereof. The present invention further relates to promoters which operate efficiently in plants such as rose, gerbera or botanically related plants.

    摘要翻译： 本发明一般涉及编码具有类黄酮3'，5'-羟化酶（F3'5'H）活性的多肽的遗传序列，以及遗传序列和/或其相应多肽的用途，尤其用于操作颜色 花或其部分或其他植物组织。 更具体地说，F3'5'H具有调节二氢卡泊芬酚（DHK）代谢以及其它底物如二氢槲皮素（DHQ），柚皮素和二碘代醇的代谢能力。 更具体地说，本发明提供编码在玫瑰或非洲菊或植物相关植物中表达时具有F3'5'H活性的多肽的遗传序列。 本发明还涉及对应于所有或部分受试者遗传序列或其转录物的反义和有义分子或RNAi诱导分子。 本发明还涉及在诸如玫瑰，非洲菊或植物相关植物的植物中有效操作的启动子。

    Cineraria-derived chromosomal DNA involved in synthesis of flavonoid, and use thereof
    10.
    发明授权
    Cineraria-derived chromosomal DNA involved in synthesis of flavonoid, and use thereof 有权
    涉及黄酮类化合物的花粉衍生染色体DNA及其用途

    公开(公告)号：US08852942B2

    公开(公告)日：2014-10-07

    申请号：US13124985

    申请日：2009-10-26

    申请人： Yoshikazu Tanaka ,  Filippa Brugliera

    发明人： Yoshikazu Tanaka ,  Filippa Brugliera

    IPC分类号： C12N15/00 ,  C12N5/04 ,  C12N5/10 ,  C12N1/20 ,  C07H21/04 ,  C12N9/02 ,  C12N15/82

    CPC分类号： C12N15/825 ,  C12N9/0073 ,  C12N15/8225

    摘要： Presently provided are a cineraria-derived polynucleotide which can act as a promoter for a coding region of flavonoid 3′,5′-hydroxylase (F3′5′H and a F3′5′H gene construct which carries the polynucleotide. The cineraria-derived petal-specific promoter can be as polynucleotide having the nucleotide sequence depicted in SEQ ID NO: 9 or a polynucleotide which can hybridize with the polynucleotide having the nucleotide sequence depicted in SEQ ID NO: 9 under stringent conditions and can act as a promoter for a coding region of flavonoid. 3′,5′-hydroxylase (F3′5′H). The gene construct can be used, for inhibiting the expression of a gene in an RNAi method, which has, as a loop, a polynucleotide comprising the whole or a part of a first intron of the presently provided cineraria-derived polynucleotide.

    摘要翻译： 目前提供的是一种能够起到促进黄酮3'，5'-羟化酶（F3'5'H）和携带多核苷酸的F3'5'H基因构建体的编码区的启动子的木质素来源的多核苷酸， 衍生花瓣特异性启动子可以是具有SEQ ID NO：9所示核苷酸序列的多核苷酸或可以在严格条件下与具有SEQ ID NO：9所示核苷酸序列的多核苷酸杂交的多核苷酸，并且可以作为 3'，5'-羟化酶（F3'5'H）的编码区，该基因构建体可以用于抑制RNAi方法中的基因的表达，该RNAi方法以环作为包含 目前提供的来自牙釉质的多核苷酸的第一内含子的全部或部分。