公开(公告)号：US08445748B2

公开(公告)日：2013-05-21

申请号：US12566357

申请日：2009-09-24

申请人： Filippa Brugliera ,  Yoshikazu Tanaka ,  John Mason

发明人： Filippa Brugliera ,  Yoshikazu Tanaka ,  John Mason

IPC分类号： A01H5/00 ,  C12N15/63 ,  C12N5/14 ,  C07H21/00

CPC分类号： C12N9/0073 ,  C12N15/825

摘要： The present invention relates generally to a genetic sequence encoding a polypeptide having flavonoid 3′,5′-hydroxylase (F3′5′H) activity and to the use of the genetic sequence and/or its corresponding polypeptide thereof inter alia to manipulate color in flowers or parts thereof or in other plant tissue. More particularly, the F3′5′H has the ability to modulate dihydrokaempferol (DHK) metabolism as well as the metabolism of other substrates such as dihydroquercetin (DHQ), naringenin and eriodictyol. Even more particularly, the present invention provides a genetic sequence encoding a polypeptide having F3′5′H activity when expressed in rose or gerbera or botanically related plants. The instant invention further relates to antisense and sense molecules or RNAi-inducing molecules corresponding to all or part of the subject genetic sequence or a transcript thereof. The present invention further relates to promoters which operate efficiently in plants such as rose, gerbera or botanically related plants.

公开(公告)号：US20070033674A1

公开(公告)日：2007-02-08

申请号：US10526133

申请日：2003-08-29

申请人： Filippa Brugliera ,  Yoshikazu Tanaka ,  John Mason

发明人： Filippa Brugliera ,  Yoshikazu Tanaka ,  John Mason

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N9/10 ,  C12N15/82 ,  C12N5/04

CPC分类号： C12N9/0073 ,  C12N15/825

摘要： The present invention relates generally to a genetic sequence encoding a polypeptide having flavonoid 3′,5′-hydroxylase (F3′5′H) activity and to the use of the genetic sequence and/or its corresponding polypeptide thereof inter alia to manipulate color in flowers or parts thereof or in other plant tissue. More particularly, the F3′5′H has the ability to modulate dihydrokaempferol (DHK) metabolism as well as the metabolism of other substrates such as dihydroquercetin (DHQ), naringenin and eriodictyol. Even more particularly, the present invention provides a genetic sequence encoding a polypeptide having F3′5′H activity when expressed in rose or gerbera or botanically related plants. The instant invention further relates to antisense and sense molecules or RNAi-inducing molecules corresponding to all or part of the subject genetic sequence or a transcript thereof. The present invention further relates to promoters which operate efficiently in plants such as rose, gerbera or botanically related plants.

摘要翻译： 本发明一般涉及编码具有类黄酮3'，5'-羟化酶（F3'5'H）活性的多肽的遗传序列，以及遗传序列和/或其相应多肽的用途，尤其用于操作颜色 花或其部分或其他植物组织。 更具体地说，F3'5'H具有调节二氢卡泊芬酚（DHK）代谢以及其它底物如二氢槲皮素（DHQ），柚皮素和二碘代醇的代谢能力。 更具体地说，本发明提供编码在玫瑰或非洲菊或植物相关植物中表达时具有F3'5'H活性的多肽的遗传序列。 本发明还涉及对应于所有或部分受试者遗传序列或其转录物的反义和有义分子或RNAi诱导分子。 本发明还涉及在诸如玫瑰，非洲菊或植物相关植物的植物中有效操作的启动子。

公开(公告)号：US07612257B2

公开(公告)日：2009-11-03

申请号：US10526133

申请日：2003-08-29

申请人： Filippa Brugliera ,  Yoshikazu Tanaka ,  John Mason

发明人： Filippa Brugliera ,  Yoshikazu Tanaka ,  John Mason

IPC分类号： A01H5/00 ,  C12N15/63 ,  C12N5/14 ,  C07H21/00

CPC分类号： C12N9/0073 ,  C12N15/825

摘要： The present invention relates generally to a genetic sequence encoding a polypeptide having flavonoid 3′,5′-hydroxylase (F3′5′H) activity and to the use of the genetic sequence and/or its corresponding polypeptide thereof inter alia to manipulate color in flowers or parts thereof or in other plant tissue. More particularly, the F3′5′H has the ability to modulate dihydrokaempferol (DHK) metabolism as well as the metabolism of other substrates such as dihydroquercetin (DHQ), naringenin and eriodictyol. Even more particularly, the present invention provides a genetic sequence encoding a polypeptide having F3′5′H activity when expressed in rose or gerbera or botanically related plants. The instant invention further relates to antisense and sense molecules or RNAi-inducing molecules corresponding to all or part of the subject genetic sequence or a transcript thereof. The present invention further relates to promoters which operate efficiently in plants such as rose, gerbera or botanically related plants.

摘要翻译： 本发明一般涉及编码具有类黄酮3'，5'-羟化酶（F3'5'H）活性的多肽的遗传序列，以及遗传序列和/或其相应多肽的用途，尤其用于操作颜色 花或其部分或其他植物组织。 更具体地说，F3'5'H具有调节二氢卡泊芬酚（DHK）代谢以及其它底物如二氢槲皮素（DHQ），柚皮素和二碘代醇的代谢能力。 更具体地说，本发明提供编码在玫瑰或非洲菊或植物相关植物中表达时具有F3'5'H活性的多肽的遗传序列。 本发明还涉及对应于所有或部分受试者遗传序列或其转录物的反义和有义分子或RNAi诱导分子。 本发明还涉及在诸如玫瑰，非洲菊或植物相关植物的植物中有效操作的启动子。

公开(公告)号：US08852942B2

公开(公告)日：2014-10-07

申请号：US13124985

申请日：2009-10-26

申请人： Yoshikazu Tanaka ,  Filippa Brugliera

发明人： Yoshikazu Tanaka ,  Filippa Brugliera

IPC分类号： C12N15/00 ,  C12N5/04 ,  C12N5/10 ,  C12N1/20 ,  C07H21/04 ,  C12N9/02 ,  C12N15/82

CPC分类号： C12N15/825 ,  C12N9/0073 ,  C12N15/8225

摘要： Presently provided are a cineraria-derived polynucleotide which can act as a promoter for a coding region of flavonoid 3′,5′-hydroxylase (F3′5′H and a F3′5′H gene construct which carries the polynucleotide. The cineraria-derived petal-specific promoter can be as polynucleotide having the nucleotide sequence depicted in SEQ ID NO: 9 or a polynucleotide which can hybridize with the polynucleotide having the nucleotide sequence depicted in SEQ ID NO: 9 under stringent conditions and can act as a promoter for a coding region of flavonoid. 3′,5′-hydroxylase (F3′5′H). The gene construct can be used, for inhibiting the expression of a gene in an RNAi method, which has, as a loop, a polynucleotide comprising the whole or a part of a first intron of the presently provided cineraria-derived polynucleotide.

摘要翻译： 目前提供的是一种能够起到促进黄酮3'，5'-羟化酶（F3'5'H）和携带多核苷酸的F3'5'H基因构建体的编码区的启动子的木质素来源的多核苷酸， 衍生花瓣特异性启动子可以是具有SEQ ID NO：9所示核苷酸序列的多核苷酸或可以在严格条件下与具有SEQ ID NO：9所示核苷酸序列的多核苷酸杂交的多核苷酸，并且可以作为 3'，5'-羟化酶（F3'5'H）的编码区，该基因构建体可以用于抑制RNAi方法中的基因的表达，该RNAi方法以环作为包含 目前提供的来自牙釉质的多核苷酸的第一内含子的全部或部分。

公开(公告)号：US20110219477A1

公开(公告)日：2011-09-08

申请号：US13124985

申请日：2009-10-26

申请人： Yoshikazu Tanaka ,  Filippa Brugliera

发明人： Yoshikazu Tanaka ,  Filippa Brugliera

IPC分类号： A01H5/00 ,  C07H21/00 ,  C12N15/63 ,  C12N1/00 ,  C12N5/10

CPC分类号： C12N15/825 ,  C12N9/0073 ,  C12N15/8225

摘要： Disclosed are: a novel cineraria-derived polynucleotide which can act as a promoter for a coding region of flavonoid 3′,5′-hydroxylase (F3′5′H); and a novel F3′5′H gene construct which carries the polynucleotide. Specifically disclosed are: a cineraria-derived petal-specific promoter which is selected from the group consisting of (1) a polynucleotide having the nucleotide sequence depicted in SEQ ID NO:9 and (2) a polynucleotide which can hybridize with the polynucleotide having the nucleotide sequence depicted in SEQ ID NO:9 under stringent conditions and can act as a promoter for a coding region of flavonoid 3′,5′-hydroxylase (F3′5′H); and a gene construct for inhibiting the expression of a gene in an RNAi method, which has, as a loop, a polynucleotide comprising the whole or a part of a first intron of the novel cineraria-derived polynucleotide.

摘要翻译： 本发明涉及一种新型的来源于线粒体的多核苷酸，其可以作为黄酮3'，5'-羟化酶（F3'5'H）的编码区的启动子。 和携带多核苷酸的新型F3'5'H基因构建体。 具体公开的是：来自花粉衍生的花瓣特异性启动子，其选自（1）具有SEQ ID NO：9所示核苷酸序列的多核苷酸和（2）可与具有SEQ ID NO：9的多核苷酸杂交的多核苷酸， 在严格条件下显示于SEQ ID NO：9中的核苷酸序列，可作为黄酮3'，5'-羟化酶（F3'5'H）编码区的启动子。 以及用于以RNAi方法抑制基因表达的基因构建体，其具有作为环的多核苷酸，所述多核苷酸包含全新一代来源于多形核苷酸的多核苷酸的第一内含子的全部或一部分。

公开(公告)号：US07807877B2

公开(公告)日：2010-10-05

申请号：US10502515

申请日：2003-01-24

申请人： Filippa Brugliera ,  Linda Demelis ,  Ronald Koes ,  Yoshikazu Tanaka

发明人： Filippa Brugliera ,  Linda Demelis ,  Ronald Koes ,  Yoshikazu Tanaka

IPC分类号： C12N15/29 ,  C12N15/82 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N9/1007 ,  C12N15/825 ,  C12N15/827

摘要： The present invention relates generally to a genetic sequence encoding a polypeptide having methyltransferase activity and the use of the genetic sequence and/or the polypeptide to modify one or more phenotypic characteristics of a plant. More particularly, the methyltransferase of the present invention acts on flavonoids, preferably wherein the flavonoid is an anthocyanin. Even more particularly, the present invention relates to a polypeptide having S-adenosyl-L-methionine:anthocyanin 3′-O-methyl-transferase or S-adenosyl-L-methionine:anthocyanin 3′,5′-O-methyltransferase activity. The present invention still further provides a genetic sequence encoding a polypeptide having methyltransferase activity derived from Petunia, Torenia Fuchsia or Plumbago or botanically related plants. The instant invention further relates to antisense and sense molecules corresponding to all or part of the subject genetic sequence as well as genetically modified plants as well as cut flowers, parts, extracts and reproductive tissue from such plants.

摘要翻译： 本发明一般涉及编码具有甲基转移酶活性的多肽的遗传序列，以及遗传序列和/或多肽修饰植物的一种或多种表型特征的用途。 更具体地，本发明的甲基转移酶作用于黄酮类化合物，优选其中类黄酮是花青素。 更具体地，本发明涉及具有S-腺苷-L-甲硫氨酸：花青素3'-O-甲基转移酶或S-腺苷-L-甲硫氨酸：花青素3'，5'-O-甲基转移酶活性的多肽。 本发明还进一步提供了编码具有衍生自矮牵牛属，Torenia Fuchsia或Plumbago或植物相关植物的甲基转移酶活性的多肽的遗传序列。 本发明还涉及对应于全部或部分受试者遗传序列以及转基因植物以及来自这些植物的切花，部分，提取物和生殖组织的反义和有义分子。

公开(公告)号：US20070150984A1

公开(公告)日：2007-06-28

申请号：US10502515

申请日：2003-01-24

申请人： Filippa Brugliera ,  Linda Demelis ,  Ronald Koes ,  Yoshikazu Tanaka

发明人： Filippa Brugliera ,  Linda Demelis ,  Ronald Koes ,  Yoshikazu Tanaka

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12P21/06 ,  C12N9/10 ,  C12N5/04

CPC分类号： C12N9/1007 ,  C12N15/825 ,  C12N15/827

摘要： The present invention relates generally to a genetic sequence encoding a polypeptide having methyltransferase activity and the use of the genetic sequence and/or the polypeptide to modify one or more phenotypic characteristics of a plant. More particularly, the methyltransferase of the present invention acts on flavonoids, preferably wherein the flavonoid is an anthocyanin. Even more particularly, the present invention relates to a polypeptide having S-adenosyl-L-methionine:anthocyanin 3′-O-methyl-transferase or S-adenosyl-L-methionine:anthocyanin 3′,5′-O-methyltransferase activity. The present invention still further provides a genetic sequence encoding a polypeptide having methyltransferase activity derived from Petunia, Torenia Fuchsia or Plumbago or botanically related plants. The instant invention further relates to antisense and sense molecules corresponding to all or part of the subject genetic sequence as well as genetically modified plants as well as cut flowers, parts, extracts and reproductive tissue from such plants.

摘要翻译： 本发明一般涉及编码具有甲基转移酶活性的多肽的遗传序列，以及遗传序列和/或多肽修饰植物的一种或多种表型特征的用途。 更具体地，本发明的甲基转移酶作用于黄酮类化合物，优选其中类黄酮是花青素。 更具体地，本发明涉及具有S-腺苷-L-甲硫氨酸：花青素3'-O-甲基转移酶或S-腺苷-L-甲硫氨酸：花青素3'，5'-O-甲基转移酶活性的多肽。 本发明还进一步提供了编码具有衍生自矮牵牛属，Torenia Fuchsia或Plumbago或植物相关植物的甲基转移酶活性的多肽的遗传序列。 本发明还涉及对应于全部或部分受试者遗传序列以及转基因植物以及来自这些植物的切花，部分，提取物和生殖组织的反义和有义分子。

公开(公告)号：US09114699B2

公开(公告)日：2015-08-25

申请号：US13883495

申请日：2011-11-07

申请人： Yasuhito Takei ,  Yoshikazu Tanaka ,  Toshiharu Kumagai ,  Ken Hayasaka ,  Masahiro Yoshizawa

发明人： Yasuhito Takei ,  Yoshikazu Tanaka ,  Toshiharu Kumagai ,  Ken Hayasaka ,  Masahiro Yoshizawa

IPC分类号： F16H37/06 ,  B60K6/50 ,  B60K6/365 ,  B60K6/445 ,  B60K6/547 ,  F16H3/72 ,  B60K6/387 ,  B60K6/48 ,  F16H37/08 ,  F16H37/10

CPC分类号： B60K6/50 ,  B60K6/365 ,  B60K6/387 ,  B60K6/445 ,  B60K6/48 ,  B60K6/547 ,  F16H3/728 ,  F16H2037/0886 ,  F16H2037/101 ,  F16H2200/0043 ,  F16H2200/0047 ,  F16H2200/0052 ,  F16H2200/2005 ,  F16H2200/2007 ,  F16H2200/2041 ,  F16H2200/2043 ,  F16H2200/2097 ,  Y02T10/6221 ,  Y02T10/6239 ,  Y10S903/902

摘要： In a hybrid drive apparatus, a first planetary gear mechanism transmits rotation of a first main shaft to which the engine driving force is inputted to a main speed-changing part at an equal speed due to engagement of a first clutch, and transmits the rotation of the first main shaft to the main speed-changing part and a first motor/generator at an increased speed due to engagement of a second clutch. This enables the range of gear ratios of the transmission to be extended and the degree of freedom in setting a gear ratio or a step ratio to be increased, and enhances the power generation efficiency by rotating the first motor/generator at a high speed without using a special speed increase device.

摘要翻译： 在混合动力驱动装置中，第一行星齿轮机构由于第一离合器的接合而以相同的速度将发动机驱动力输入到的主变速部的第一主轴的旋转传递到主变速部， 主变速部分的第一主轴和由于第二离合器的接合而以增加的速度的第一电动机/发电机。 这样可以使传动装置的传动比范围扩大，并且使齿轮比或步进比的设定自由度提高，并且通过高速旋转第一电动机/发电机而提高发电效率，而不使用 特殊加速装置。

公开(公告)号：US09057076B2

公开(公告)日：2015-06-16

申请号：US13259749

申请日：2010-03-25

申请人： Yuko Fukui ,  Yoshikazu Tanaka

发明人： Yuko Fukui ,  Yoshikazu Tanaka

IPC分类号： C07D311/62 ,  C12N15/82 ,  A01H5/02 ,  C07D493/16 ,  C07H13/08 ,  C12N9/02 ,  C12N9/10

CPC分类号： C12N15/825 ,  A01H5/02 ,  A01H6/749 ,  C07D493/16 ,  C07H13/08 ,  C12N9/0071 ,  C12N9/0073 ,  C12N9/1029

摘要： Disclosed is a novel compound contained in blue rose. This novel compound, which is contained in blue rose, has a chemical structure represented by general formula (I): [wherein R1 is a group as set forth in claim 1; and R2 represents —OH, or R1 and R2 together form —O—]. Also disclosed are a rose plant containing the aforesaid compound, and a part of the same.

摘要翻译： 公开了蓝玫瑰中含有的新化合物。 包含在蓝玫瑰中的这种新化合物具有由通式（I）表示的化学结构：[其中R1是如权利要求1所述的基团; 并且R 2表示-OH，或者R 1和R 2一起形成-O-]。 还公开了含有上述化合物及其一部分的玫瑰植物。

公开(公告)号：US09011396B2

公开(公告)日：2015-04-21

申请号：US13578843

申请日：2011-02-17

申请人： Kaiyo Nakajima ,  Haruki Toda ,  Yoshikazu Tanaka

发明人： Kaiyo Nakajima ,  Haruki Toda ,  Yoshikazu Tanaka

IPC分类号： A61F13/15 ,  A61F13/49 ,  A61F13/511 ,  A61F13/74 ,  A61F13/76 ,  A61F13/494 ,  A61F13/513 ,  A61F13/00

CPC分类号： A61F13/49011 ,  A61F13/49466 ,  A61F13/5116 ,  A61F13/513 ,  A61F2013/00404 ,  A61F2013/15024 ,  A61F2013/15154 ,  A61F2013/51182 ,  A61F2013/51316

摘要： A disposable wearing article has a skin-contactable sheet moveable relative to a chassis. The chassis includes an inner sheet, an outer sheet and a liquid-absorbent panel interposed between these inner and outer sheets. The absorbent panel lies at least in the crotch region and extends into the front and rear waist regions in the longitudinal direction. In the rear waist region, a skin-contactable sheet adapted to come in contact with the wearer's skin is attached to the inner surface of the inner sheet. The skin-contactable sheet has front and rear ends extending in the transverse direction and lateral portions extending in the longitudinal direction and only the lateral portions are bonded to the inner sheet.

摘要翻译： 一次性穿着物品具有可相对于底盘移动的皮肤接触片材。 底盘包括内片，外片和介于这些内片和外片之间的吸液性片。 吸收性面板至少位于裆下区域中并且在纵向方向上延伸到前后腰部区域。 在后腰部区域中，与皮肤接触的皮肤接触片附着到内片的内表面。 皮肤接触片具有在横向方向上延伸的前端和后端以及在纵向方向上延伸的侧向部分，并且只有侧部结合到内片。