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公开(公告)号：US20070033674A1
公开(公告)日：2007-02-08
申请号：US10526133
申请日：2003-08-29
申请人： Filippa Brugliera , Yoshikazu Tanaka , John Mason
发明人： Filippa Brugliera , Yoshikazu Tanaka , John Mason
CPC分类号： C12N9/0073 , C12N15/825
摘要： The present invention relates generally to a genetic sequence encoding a polypeptide having flavonoid 3′,5′-hydroxylase (F3′5′H) activity and to the use of the genetic sequence and/or its corresponding polypeptide thereof inter alia to manipulate color in flowers or parts thereof or in other plant tissue. More particularly, the F3′5′H has the ability to modulate dihydrokaempferol (DHK) metabolism as well as the metabolism of other substrates such as dihydroquercetin (DHQ), naringenin and eriodictyol. Even more particularly, the present invention provides a genetic sequence encoding a polypeptide having F3′5′H activity when expressed in rose or gerbera or botanically related plants. The instant invention further relates to antisense and sense molecules or RNAi-inducing molecules corresponding to all or part of the subject genetic sequence or a transcript thereof. The present invention further relates to promoters which operate efficiently in plants such as rose, gerbera or botanically related plants.
摘要翻译： 本发明一般涉及编码具有类黄酮3'，5'-羟化酶（F3'5'H）活性的多肽的遗传序列，以及遗传序列和/或其相应多肽的用途，尤其用于操作颜色 花或其部分或其他植物组织。 更具体地说，F3'5'H具有调节二氢卡泊芬酚（DHK）代谢以及其它底物如二氢槲皮素（DHQ），柚皮素和二碘代醇的代谢能力。 更具体地说，本发明提供编码在玫瑰或非洲菊或植物相关植物中表达时具有F3'5'H活性的多肽的遗传序列。 本发明还涉及对应于所有或部分受试者遗传序列或其转录物的反义和有义分子或RNAi诱导分子。 本发明还涉及在诸如玫瑰，非洲菊或植物相关植物的植物中有效操作的启动子。
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公开(公告)号：US08445748B2
公开(公告)日：2013-05-21
申请号：US12566357
申请日：2009-09-24
申请人： Filippa Brugliera , Yoshikazu Tanaka , John Mason
发明人： Filippa Brugliera , Yoshikazu Tanaka , John Mason
CPC分类号： C12N9/0073 , C12N15/825
摘要： The present invention relates generally to a genetic sequence encoding a polypeptide having flavonoid 3′,5′-hydroxylase (F3′5′H) activity and to the use of the genetic sequence and/or its corresponding polypeptide thereof inter alia to manipulate color in flowers or parts thereof or in other plant tissue. More particularly, the F3′5′H has the ability to modulate dihydrokaempferol (DHK) metabolism as well as the metabolism of other substrates such as dihydroquercetin (DHQ), naringenin and eriodictyol. Even more particularly, the present invention provides a genetic sequence encoding a polypeptide having F3′5′H activity when expressed in rose or gerbera or botanically related plants. The instant invention further relates to antisense and sense molecules or RNAi-inducing molecules corresponding to all or part of the subject genetic sequence or a transcript thereof. The present invention further relates to promoters which operate efficiently in plants such as rose, gerbera or botanically related plants.
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公开(公告)号：US07612257B2
公开(公告)日：2009-11-03
申请号：US10526133
申请日：2003-08-29
申请人： Filippa Brugliera , Yoshikazu Tanaka , John Mason
发明人： Filippa Brugliera , Yoshikazu Tanaka , John Mason
CPC分类号： C12N9/0073 , C12N15/825
摘要： The present invention relates generally to a genetic sequence encoding a polypeptide having flavonoid 3′,5′-hydroxylase (F3′5′H) activity and to the use of the genetic sequence and/or its corresponding polypeptide thereof inter alia to manipulate color in flowers or parts thereof or in other plant tissue. More particularly, the F3′5′H has the ability to modulate dihydrokaempferol (DHK) metabolism as well as the metabolism of other substrates such as dihydroquercetin (DHQ), naringenin and eriodictyol. Even more particularly, the present invention provides a genetic sequence encoding a polypeptide having F3′5′H activity when expressed in rose or gerbera or botanically related plants. The instant invention further relates to antisense and sense molecules or RNAi-inducing molecules corresponding to all or part of the subject genetic sequence or a transcript thereof. The present invention further relates to promoters which operate efficiently in plants such as rose, gerbera or botanically related plants.
摘要翻译： 本发明一般涉及编码具有类黄酮3'，5'-羟化酶（F3'5'H）活性的多肽的遗传序列，以及遗传序列和/或其相应多肽的用途，尤其用于操作颜色 花或其部分或其他植物组织。 更具体地说，F3'5'H具有调节二氢卡泊芬酚（DHK）代谢以及其它底物如二氢槲皮素（DHQ），柚皮素和二碘代醇的代谢能力。 更具体地说，本发明提供编码在玫瑰或非洲菊或植物相关植物中表达时具有F3'5'H活性的多肽的遗传序列。 本发明还涉及对应于所有或部分受试者遗传序列或其转录物的反义和有义分子或RNAi诱导分子。 本发明还涉及在诸如玫瑰，非洲菊或植物相关植物的植物中有效操作的启动子。
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4.发明授权Cineraria-derived chromosomal DNA involved in synthesis of flavonoid, and use thereof 有权涉及黄酮类化合物的花粉衍生染色体DNA及其用途公开(公告)号：US08852942B2
公开(公告)日：2014-10-07
申请号：US13124985
申请日：2009-10-26
CPC分类号： C12N15/825 , C12N9/0073 , C12N15/8225
摘要： Presently provided are a cineraria-derived polynucleotide which can act as a promoter for a coding region of flavonoid 3′,5′-hydroxylase (F3′5′H and a F3′5′H gene construct which carries the polynucleotide. The cineraria-derived petal-specific promoter can be as polynucleotide having the nucleotide sequence depicted in SEQ ID NO: 9 or a polynucleotide which can hybridize with the polynucleotide having the nucleotide sequence depicted in SEQ ID NO: 9 under stringent conditions and can act as a promoter for a coding region of flavonoid. 3′,5′-hydroxylase (F3′5′H). The gene construct can be used, for inhibiting the expression of a gene in an RNAi method, which has, as a loop, a polynucleotide comprising the whole or a part of a first intron of the presently provided cineraria-derived polynucleotide.
摘要翻译： 目前提供的是一种能够起到促进黄酮3'，5'-羟化酶（F3'5'H）和携带多核苷酸的F3'5'H基因构建体的编码区的启动子的木质素来源的多核苷酸， 衍生花瓣特异性启动子可以是具有SEQ ID NO：9所示核苷酸序列的多核苷酸或可以在严格条件下与具有SEQ ID NO：9所示核苷酸序列的多核苷酸杂交的多核苷酸，并且可以作为 3'，5'-羟化酶（F3'5'H）的编码区，该基因构建体可以用于抑制RNAi方法中的基因的表达，该RNAi方法以环作为包含 目前提供的来自牙釉质的多核苷酸的第一内含子的全部或部分。
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5.发明申请CINERARIUM-DERIVED CHROMOSOMAL DNA INVOLVED IN SYNTHESIS OF FLAVONOID, AND USE THEREOF 有权参与合成黄曲霉毒素的衍生染色体DNA及其用途公开(公告)号：US20110219477A1
公开(公告)日：2011-09-08
申请号：US13124985
申请日：2009-10-26
CPC分类号： C12N15/825 , C12N9/0073 , C12N15/8225
摘要： Disclosed are: a novel cineraria-derived polynucleotide which can act as a promoter for a coding region of flavonoid 3′,5′-hydroxylase (F3′5′H); and a novel F3′5′H gene construct which carries the polynucleotide. Specifically disclosed are: a cineraria-derived petal-specific promoter which is selected from the group consisting of (1) a polynucleotide having the nucleotide sequence depicted in SEQ ID NO:9 and (2) a polynucleotide which can hybridize with the polynucleotide having the nucleotide sequence depicted in SEQ ID NO:9 under stringent conditions and can act as a promoter for a coding region of flavonoid 3′,5′-hydroxylase (F3′5′H); and a gene construct for inhibiting the expression of a gene in an RNAi method, which has, as a loop, a polynucleotide comprising the whole or a part of a first intron of the novel cineraria-derived polynucleotide.
摘要翻译： 本发明涉及一种新型的来源于线粒体的多核苷酸，其可以作为黄酮3'，5'-羟化酶（F3'5'H）的编码区的启动子。 和携带多核苷酸的新型F3'5'H基因构建体。 具体公开的是：来自花粉衍生的花瓣特异性启动子，其选自（1）具有SEQ ID NO：9所示核苷酸序列的多核苷酸和（2）可与具有SEQ ID NO：9的多核苷酸杂交的多核苷酸， 在严格条件下显示于SEQ ID NO：9中的核苷酸序列，可作为黄酮3'，5'-羟化酶（F3'5'H）编码区的启动子。 以及用于以RNAi方法抑制基因表达的基因构建体，其具有作为环的多核苷酸，所述多核苷酸包含全新一代来源于多形核苷酸的多核苷酸的第一内含子的全部或一部分。
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公开(公告)号：US07807877B2
公开(公告)日：2010-10-05
申请号：US10502515
申请日：2003-01-24
CPC分类号： C12N9/1007 , C12N15/825 , C12N15/827
摘要： The present invention relates generally to a genetic sequence encoding a polypeptide having methyltransferase activity and the use of the genetic sequence and/or the polypeptide to modify one or more phenotypic characteristics of a plant. More particularly, the methyltransferase of the present invention acts on flavonoids, preferably wherein the flavonoid is an anthocyanin. Even more particularly, the present invention relates to a polypeptide having S-adenosyl-L-methionine:anthocyanin 3′-O-methyl-transferase or S-adenosyl-L-methionine:anthocyanin 3′,5′-O-methyltransferase activity. The present invention still further provides a genetic sequence encoding a polypeptide having methyltransferase activity derived from Petunia, Torenia Fuchsia or Plumbago or botanically related plants. The instant invention further relates to antisense and sense molecules corresponding to all or part of the subject genetic sequence as well as genetically modified plants as well as cut flowers, parts, extracts and reproductive tissue from such plants.
摘要翻译： 本发明一般涉及编码具有甲基转移酶活性的多肽的遗传序列，以及遗传序列和/或多肽修饰植物的一种或多种表型特征的用途。 更具体地，本发明的甲基转移酶作用于黄酮类化合物，优选其中类黄酮是花青素。 更具体地，本发明涉及具有S-腺苷-L-甲硫氨酸：花青素3'-O-甲基转移酶或S-腺苷-L-甲硫氨酸：花青素3'，5'-O-甲基转移酶活性的多肽。 本发明还进一步提供了编码具有衍生自矮牵牛属，Torenia Fuchsia或Plumbago或植物相关植物的甲基转移酶活性的多肽的遗传序列。 本发明还涉及对应于全部或部分受试者遗传序列以及转基因植物以及来自这些植物的切花，部分，提取物和生殖组织的反义和有义分子。
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公开(公告)号：US20070150984A1
公开(公告)日：2007-06-28
申请号：US10502515
申请日：2003-01-24
CPC分类号： C12N9/1007 , C12N15/825 , C12N15/827
摘要： The present invention relates generally to a genetic sequence encoding a polypeptide having methyltransferase activity and the use of the genetic sequence and/or the polypeptide to modify one or more phenotypic characteristics of a plant. More particularly, the methyltransferase of the present invention acts on flavonoids, preferably wherein the flavonoid is an anthocyanin. Even more particularly, the present invention relates to a polypeptide having S-adenosyl-L-methionine:anthocyanin 3′-O-methyl-transferase or S-adenosyl-L-methionine:anthocyanin 3′,5′-O-methyltransferase activity. The present invention still further provides a genetic sequence encoding a polypeptide having methyltransferase activity derived from Petunia, Torenia Fuchsia or Plumbago or botanically related plants. The instant invention further relates to antisense and sense molecules corresponding to all or part of the subject genetic sequence as well as genetically modified plants as well as cut flowers, parts, extracts and reproductive tissue from such plants.
摘要翻译： 本发明一般涉及编码具有甲基转移酶活性的多肽的遗传序列，以及遗传序列和/或多肽修饰植物的一种或多种表型特征的用途。 更具体地，本发明的甲基转移酶作用于黄酮类化合物，优选其中类黄酮是花青素。 更具体地，本发明涉及具有S-腺苷-L-甲硫氨酸：花青素3'-O-甲基转移酶或S-腺苷-L-甲硫氨酸：花青素3'，5'-O-甲基转移酶活性的多肽。 本发明还进一步提供了编码具有衍生自矮牵牛属，Torenia Fuchsia或Plumbago或植物相关植物的甲基转移酶活性的多肽的遗传序列。 本发明还涉及对应于全部或部分受试者遗传序列以及转基因植物以及来自这些植物的切花，部分，提取物和生殖组织的反义和有义分子。
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公开(公告)号：US20060107351A1
公开(公告)日：2006-05-18
申请号：US11118112
申请日：2005-04-29
申请人： Mirko Karan , Filippa Brugliera , John Mason , Sophie Dove , Ian Hoegh-Guldberg , Mark Prescott
发明人： Mirko Karan , Filippa Brugliera , John Mason , Sophie Dove , Ian Hoegh-Guldberg , Mark Prescott
IPC分类号： A01H1/00 , C12Q1/68 , C07H21/04 , C07K14/415 , C12N1/21
CPC分类号： C12Q1/6897 , A61K8/64 , A61K2800/86 , A61Q17/04 , C07K14/43595
摘要： The present invention relates generally to peptides, polypeptides or proteins having one or more amino acids or one or more amino acid sequences which exhibit color-facilitating properties, either on their own or following interaction with one or more other amino acids and to nucleic acid molecules encoding same. Such peptides, polypeptides and proteins are referred to herein as “color-facilitating molecules” or “CFMs”. The present invention further provides genetic constructs for use in genetically modifying eukaryotic or prokaryotic cells and more particularly eukaryotic tissue so as to alter their visual characteristics or capacity for exhibiting same to a human eye in the absence of excitation by an extraneous non-white light or particle emission. The present invention, therefore, extends to eukaryotic or prokaryotic cells and more particularly eukaryotic tissue, which are genetically modified to produce CFMs and which thereby exhibit altered visual characteristics in the absence of excitation by an extraneous non-white light or particle emission. In one particular embodiment, the CFMs are used to alter the visual characteristics of plants and even more particularly flower color. In another embodiment, the present invention provides gels or coatings or similar biomaterials in the form of a biomatrix comprising the CFMs such as for use as a UV sink, in a sun screen, in cosmetics, as an expression marker or other reporter molecule or for use as a photon trap to increase light intensity.
摘要翻译： 本发明一般涉及具有一个或多个氨基酸或一个或多个氨基酸序列的肽，多肽或蛋白质，其表现出颜色促进性质，其本身或之后与一种或多种其它氨基酸和核酸分子的相互作用 编码相同。 这样的肽，多肽和蛋白质在本文中称为“促色分子”或“CFM”。 本发明还提供用于遗传修饰真核细胞或原核细胞，更特别是真核组织的遗传构建体，以便在没有外来非白光的激发的情况下改变其在人眼上显示相同的视觉特征或能力 颗粒发射。 因此，本发明延伸到真核细胞或原核细胞，更特别是真核组织，其被遗传修饰以产生CFM，并且由此在没有通过外来的非白光或颗粒发射的激发的情况下表现出改变的视觉特征。 在一个具体实施方案中，CFM用于改变植物的视觉特征，甚至更特别地是花色。 在另一个实施方案中，本发明提供了生物基质形式的凝胶或涂层或类似生物材料，其包含CFM，例如用作UV吸收池，在日光屏中，在化妆品中，作为表达标记物或其他报告分子，或用于 用作光子阱以增加光强度。
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公开(公告)号：USPP21515P2
公开(公告)日：2010-11-23
申请号：US12454905
申请日：2009-05-26
申请人： Filippa Brugliera
发明人： Filippa Brugliera
IPC分类号： A01H5/00
CPC分类号： A01H5/02
摘要： A new cultivar of Dianthus plant named ‘FLORIJADE’ is characterized, inter alia, by pronounced spray habit, perennial and profuse bloom, and green foliage. Dianthus ‘FLORIJADE’ is suitable for use, for example, as a flowering plant in pots, containers, window boxes, and the garden, but is primarily suited for the production of cut flowers. Dianthus ‘FLORIJADE’ is not hardy and is typically grown in a glasshouse. These traits set ‘FLORIJADE’ apart from all existing varieties, lines, strains, or sports of Dianthus. In particular, Dianthus ‘FLORIJADE’ has bright purple/violet flowers.
摘要翻译： 名为“FLORIJADE”的石竹植物新品种的特征是特别是喷雾习性显着，多年生植物和多汁的植物和绿色的叶子。 石竹'FLORIJADE'适用于例如花盆，容器，窗框和花园中的开花植物，但主要适用于生产切花。 石竹'FLORIJADE'不硬，通常种植在温室中。 除了所有现有品种，线条，品种或运动的石竹之外，这些特征都设定了“佛罗伦萨”。 特别地，石竹'FLORIJADE'具有明亮的紫色/紫罗兰花。
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10.发明申请公开(公告)号：US20050081264A1
公开(公告)日：2005-04-14
申请号：US10669962
申请日：2003-09-24
IPC分类号： A01H5/00 , A01H5/02 , C12N9/02 , C12N15/09 , C12N15/29 , C12N15/63 , C12N15/82 , A01H1/00 , C07H21/04
CPC分类号： C12N9/0071 , C12N15/825
摘要： The present invention relates generally to genetic sequences encoding flavonoid pathway metabolising enzymes and more particularly to flavonoid 3′-hydroxylase (hereinafter referred to as “F3′H”) or derivatives thereof and their use in the manipulation of pigmentation in plants and other organisms.
摘要翻译： 本发明一般涉及编码类黄酮途径代谢酶的基因序列，更具体地涉及类黄酮3'-羟化酶（以下称为“F3'H”）或其衍生物，以及它们在植物和其他生物体内操作色素沉着的用途。
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