公开(公告)号：US07924027B2

公开(公告)日：2011-04-12

申请号：US11855752

申请日：2007-09-14

申请人： Frank Sieckmann ,  Reiner Rygiel

发明人： Frank Sieckmann ,  Reiner Rygiel

IPC分类号： G01R27/26

CPC分类号： G01B7/023

摘要： An arrangement and method are provided for determining the distance between an objective of a microscope and a sample examined with the microscope. Fitted on the objective or in the immediate vicinity of the objective is a capacitive sensor in whose measuring range the sample and/or a microscope slide supporting the sample is located or into which it can be brought. The sample and/or the slide causing a measurable change in the capacitance of the sensor. From the change in the capacitance of the sensor, the distance of the sample and/or of the microscope slide from the sensor is determined, and thus the distance of the sample from the objective. A capacitive distance sensor can be applied in such an arrangement by which a stray field can be generated between at least two electrodes. The capacitance experiences a measurable change owing to an object introduced into the stray field. The electrodes of the sensor are arranged as substantially coaxial lateral surfaces. The electrodes are designed such that the sensor can be plugged onto the objective of the microscope, the lateral surfaces at least partially enclosing and touching the objective.

摘要翻译： 提供了一种用于确定显微镜的物镜与用显微镜检查的样品之间的距离的布置和方法。 根据目标或目标附近，电容传感器的测量范围是支撑样品的样品和/或显微镜载玻片，或者可以将其带入。 样品和/或滑块导致传感器电容的可测量变化。 根据传感器电容的变化，确定样品和/或显微镜载玻片距离传感器的距离，从而确定样品与物镜的距离。 电容距离传感器可以应用于这样的布置，通过该布置可以在至少两个电极之间产生杂散场。 由于引入到杂散场中的物体，电容经历可测量的变化。 传感器的电极被布置为基本上同轴的侧表面。 电极被设计成使得传感器可以插入到显微镜的物镜上，侧表面至少部分地包围并接触物镜。

公开(公告)号：US20080068028A1

公开(公告)日：2008-03-20

申请号：US11855752

申请日：2007-09-14

申请人： Frank Sieckmann ,  Reiner Rygiel

发明人： Frank Sieckmann ,  Reiner Rygiel

IPC分类号： G01R27/28 ,  G01R27/26 ,  G06F19/00

CPC分类号： G01B7/023

摘要： An arrangement and method are provided for determining the distance between an objective of a microscope and a sample examined with the microscope. Fitted on the objective or in the immediate vicinity of the objective is a capacitive sensor in whose measuring range the sample and/or a microscope slide supporting the sample is located or into which it can be brought. The sample and/or the slide causing a measurable change in the capacitance of the sensor. From the change in the capacitance of the sensor, the distance of the sample and/or of the microscope slide from the sensor is determined, and thus the distance of the sample from the objective. A capacitive distance sensor can be applied in such an arrangement by which a stray field can be generated between at least two electrodes. The capacitance experiences a measurable change owing to an object introduced into the stray field. The electrodes of the sensor are arranged as substantially coaxial lateral surfaces. The electrodes are designed such that the sensor can be plugged onto the objective of the microscope, the lateral surfaces at least partially enclosing and touching the objective.

摘要翻译： 提供了一种用于确定显微镜的物镜与用显微镜检查的样品之间的距离的布置和方法。 根据目标或目标附近，电容传感器的测量范围是支撑样品的样品和/或显微镜载玻片，或者可以将其带入。 样品和/或滑块导致传感器电容的可测量变化。 根据传感器电容的变化，确定样品和/或显微镜载玻片距离传感器的距离，从而确定样品与物镜的距离。 电容距离传感器可以应用于这样的布置，通过该布置可以在至少两个电极之间产生杂散场。 由于引入到杂散场中的物体，电容经历可测量的变化。 传感器的电极被布置为基本上同轴的侧表面。 电极被设计成使得传感器可以插入到显微镜的物镜上，侧表面至少部分地包围并接触物镜。

公开(公告)号：US09588329B2

公开(公告)日：2017-03-07

申请号：US12439597

申请日：2007-09-06

申请人： Frank Sieckmann ,  Urban Liebel

发明人： Frank Sieckmann ,  Urban Liebel

IPC分类号： H04N9/47 ,  G02B21/36 ,  G01N15/14 ,  G06F19/00

CPC分类号： G02B21/0084 ,  G01N15/1475 ,  G02B21/367 ,  G06F19/00 ,  G06F19/3418 ,  G06K9/00134 ,  G06K9/00147

摘要： A method for scanning a sample using an electrically or electronically controllable microscope, includes scanning the sample so as to generate a plurality of images of the sample, each of the plurality of images corresponding to at least one of a different region of the sample and a different time. The microscope is controlled via a control computer during the scanning. The plurality of images are analyzed using at least one second computer connected via a network. The plurality of images are classified and/or the scanning is influenced based on the analyzing.

摘要翻译： 一种使用电子或电子可控显微镜扫描样品的方法，包括扫描样品以产生样品的多个图像，所述多个图像中的每一个对应于样品的不同区域中的至少一个和 不同的时间 显微镜在扫描期间通过控制计算机进行控制。 使用经由网络连接的至少一个第二计算机来分析多个图像。 基于分析对多个图像进行分类和/或扫描受到影响。

公开(公告)号：US09217694B2

公开(公告)日：2015-12-22

申请号：US10576453

申请日：2004-10-21

申请人： Frank Sieckmann ,  Gerhard Johannsen

发明人： Frank Sieckmann ,  Gerhard Johannsen

IPC分类号： B23K26/00 ,  B23K26/16 ,  A61B18/16 ,  G01N1/28 ,  G02B21/32 ,  G02B21/36

CPC分类号： G01N1/2813 ,  G01N2001/288 ,  G02B21/32 ,  G02B21/365

摘要： A laser microdissection method includes capturing an electronic image of an image detail of a specimen. The image detail is processed using, image analysis so as to automatically ascertain an object to be cut out. A nominal cutting line around the object to be cut out is automatically defined. Subsequently, the object is cut out in response to a relative motion between a laser beam and the specimen.

摘要翻译： 激光显微解剖方法包括拍摄样本的图像细节的电子图像。 使用图像分析处理图像细节，以便自动确定要剪切的对象。 自动定义待切割物体周围的标称切割线。 随后，响应于激光束和样本之间的相对运动来切割物体。

公开(公告)号：US20060056018A1

公开(公告)日：2006-03-16

申请号：US11225810

申请日：2005-09-13

申请人： Frank Sieckmann

发明人： Frank Sieckmann

IPC分类号： G02B21/06

CPC分类号： G02B27/144 ,  G02B21/06 ,  G02B27/145

摘要： An illumination apparatus for an optical system, in particular for a microscope, having a light source (2) emitting preferably broad-band light in order to furnish an output light beam (3), and having a filter device (5), is characterized, in the interest of economical, low-maintenance, and vibration-free generation of an illuminating light beam encompassing multiple wavelengths or wavelength regions, and a high degree of flexibility in terms of the spectral light shaping of the illuminating light beam, in that the filter device (5) encompasses at least one means (7) for spatial splitting of the output light beam (3) into light sub-beams (8, 9) and at least one means for spectral manipulation of at least one of the light sub-beams (8, 9); and that at least one beam combining means is provided with which definable light sub-beams (9) are combinable into one illuminating light beam (14) for the optical system.

摘要翻译： 用于光学系统的照明装置，特别是用于显微镜的具有发射优选宽带光的光源（2）以便提供输出光束（3）并具有滤光器装置（5）的光学装置的特征在于 ，为了节约，低维护和无振动地产生包围多个波长或波长区域的照明光束，并且在照明光束的光谱光整形方面具有高度的灵活性，因为 滤光器装置（5）包括用于将输出光束（3）空间分裂成光子束（8,9）的至少一个装置（7）和至少一个用于对光子束中的至少一个进行光谱操纵的装置 （8,9）; 并且至少一个光束组合装置设置有哪些可定义的光子束（9）可组合成用于光学系统的一个照明光束（14）。

公开(公告)号：US06693716B2

公开(公告)日：2004-02-17

申请号：US09973983

申请日：2001-10-11

申请人： Frank Sieckmann

发明人： Frank Sieckmann

IPC分类号： G01B1124

CPC分类号： G01B11/24

摘要： The object of the invention is a method and an apparatus for optical measurement of a surface profile of a specimen, a series of n images of the specimen being acquired with an image acquisition apparatus in different planes in the z direction of a coordinate system (x, y, z). The image contents of all n images of the resulting image stack are compared to each other in the z direction at each coordinate point (x, y) in order to determine a plane therefrom according to predetermined criteria, assign its plane number (N) to that coordinate point (x, y), and store it in a mask image. The mask image contains all the 3D data of the specimen surface. Processing can be performed using 2D image processing procedures. The 3D information can be quickly and easily retrieved from the mask image. The surface profile can be reconstructed and displayed three-dimensionally.

摘要翻译： 本发明的目的是一种用于光学测量样品的表面轮廓的方法和装置，用坐标系的z方向上的不同平面中的图像获取装置获取样本的一系列n个图像（x ，y，z）。 将所得到的图像堆叠的所有n个图像的图像内容在每个坐标点（x，y）处的z方向上彼此进行比较，以便根据预定标准确定其平面，将其平面数（N）分配给 该坐标点（x，y），并将其存储在掩模图像中。 掩模图像包含样品表面的所有3D数据。 可以使用2D图像处理程序来执行处理。 可以从掩模图像快速方便地检索3D信息。 表面轮廓可以三维重建和显示。

公开(公告)号：US08199407B2

公开(公告)日：2012-06-12

申请号：US12439619

申请日：2007-09-07

申请人： Urban Liebel ,  Siegfried Winkler ,  Frank Sieckmann

发明人： Urban Liebel ,  Siegfried Winkler ,  Frank Sieckmann

IPC分类号： G02B21/02

CPC分类号： G02B21/24 ,  G02B21/33 ,  G02B27/0006

摘要： An immersion objective for microscopic investigation of a specimen is provided wherein an outer lens is disposed in an objective body. A delivery device including a cap that is disposed over the objective body so as to form a space adapted to receive an immersion liquid is also provided. The cap is open in a region of the outer lens so as to form a gap with the outer lens. The cap includes at least one connector configured to provide a continuous supply of the immersion liquid to the space so that the immersion liquid emerges through the gap to a region between the outer lens and at least one of the specimen and a specimen slide.

摘要翻译： 提供了一种用于显微镜检查试样的浸没物镜，其中外透镜设置在物体中。 还提供一种包括设置在物体上方以形成适于接收浸没液体的空间的盖的输送装置。 帽在外透镜的区域中打开，以与外透镜形成间隙。 所述盖包括至少一个连接器，其被配置为提供所述浸没液体到所述空间的连续供应，使得所述浸没液体通过所述间隙泄漏到所述外部透镜与所述样本和样本载玻片中的至少一个之间的区域。

公开(公告)号：US07760428B2

公开(公告)日：2010-07-20

申请号：US11570488

申请日：2005-06-10

申请人： Frank Sieckmann

发明人： Frank Sieckmann

IPC分类号： G02B21/26

CPC分类号： G01N35/00029 ,  G01N35/00732 ,  G01N35/00871 ,  G01N2035/00138 ,  G01N2035/00782

摘要： A specimen slide unit for holding a specimen that is to be examined under a microscope or analyzed with a laboratory analysis system includes a specimen slide, a data carrier device and a signal element. The data carrier device has a radio frequency identification tag that can be read out and/or written on using Phase Jitter Modulation so as to switch a communication carrier frequency between two predefinable communication carrier frequencies. The signal element can be activated so as to assist an operator in following a predefinable processing sequence including a second specimen slide unit when the RFID tag is in a range of action of the read/write device. The signal element can be a visual or an acoustic signal element.

摘要翻译： 用于在显微镜下保持要检查的样本或用实验室分析系统分析的样本滑块单元包括样本滑块，数据载体装置和信号元件。 数据载体装置具有能够使用相位抖动调制读出和/或写入的射频识别标签，以便在两个可预定的通信载波频率之间切换通信载波频率。 信号元件可以被激活，以便当RFID标签处于读/写设备的动作范围内时，帮助操作者遵循包括第二标本滑动单元的可预定的处理顺序。 信号元件可以是视觉或声学信号元件。

公开(公告)号：US20100027109A1

公开(公告)日：2010-02-04

申请号：US12439619

申请日：2007-09-07

申请人： Urban Liebel ,  Siegfried Winkler ,  Frank Sieckmann

发明人： Urban Liebel ,  Siegfried Winkler ,  Frank Sieckmann

IPC分类号： G02B21/02 ,  G02B21/33

CPC分类号： G02B21/24 ,  G02B21/33 ,  G02B27/0006

摘要： An immersion objective for microscopic investigation of a specimen is provided wherein an outer lens is disposed in an objective body. A delivery device including a cap that is disposed over the objective body so as to form a space adapted to receive an immersion liquid is also provided. The cap is open in a region of the outer lens so as to form a gap with the outer lens. The cap includes at least one connector configured to provide a continuous supply of the immersion liquid to the space so that the immersion liquid emerges through the gap to a region between the outer lens and at least one of the specimen and a specimen slide.

摘要翻译： 提供了一种用于显微镜检查试样的浸没物镜，其中外透镜设置在物体中。 还提供一种包括设置在物体上方以形成适于接收浸没液体的空间的盖的输送装置。 帽在外透镜的区域中打开，以与外透镜形成间隙。 所述盖包括至少一个连接器，其被配置为提供所述浸没液体到所述空间的连续供应，使得所述浸没液体通过所述间隙泄漏到所述外部透镜与所述样本和样本载玻片中的至少一个之间的区域。

公开(公告)号：US20070097493A1

公开(公告)日：2007-05-03

申请号：US11535861

申请日：2006-09-27

申请人： Frank Sieckmann

发明人： Frank Sieckmann

IPC分类号： G02B21/00

CPC分类号： G02B21/24

摘要： Disclosed is an image acquisition unit (1) for microscopic applications, including at least one microscope objective (7) which is mounted on a housing. The housing (9) is formed by a plurality of individual separate modules, said plurality of modules including at least an electronic module (2), a camera module (4), an illumination module (6), a focusing module (8), and a filter module (10).

摘要翻译： 公开了一种用于微观应用的图像获取单元（1），其包括安装在壳体上的至少一个显微镜物镜（7）。 壳体（9）由多个单独的分开的模块形成，所述多个模块至少包括电子模块（2），相机模块（4），照明模块（6），聚焦模块（8） 和过滤器模块（10）。