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1.发明申请HETEROLOGOUS EXPRESSION OF EXTREMOPHILE HEAT SHOCK PROTEINS AND CHAPERONES IN MICROORGANISMS TO INCREASE TOLERANCE TO TOXIC COMPOUNDS 有权微生物中超临界热休克蛋白和葛根酮的异位表达增加对毒性化合物的耐受性公开(公告)号：US20110045554A1
公开(公告)日：2011-02-24
申请号：US12528620
申请日：2008-02-26
IPC分类号： C12P7/64 , C12P1/00 , C12P7/26 , C12P7/16 , C12P7/06 , C12P5/00 , C12P1/04 , C12P1/02 , C12N15/63 , C12N1/00 , C12N1/21 , C12N1/15 , C12N1/19 , C12N1/13 , C12N15/81 , C12N15/74 , C12N15/80 , C12N15/70
CPC分类号： C07K14/195 , Y02E50/17
摘要： The invention provides methods and compositions for increasing tolerance of microorganisms to toxic agents, such as solvents; and for increasing production of solvents from solvent-generating microorganisms. The methods comprise engineering a microorganism of interest to express a heterologous heat-shock protein/chaperone, e.g., Group II chaperonin or a prefoldin such as γ-prefoldin, where the heterologous protein is from an extremophile, such as an archaean.
摘要翻译： 本发明提供了增加微生物对毒性剂如溶剂的耐受性的方法和组合物; 并用于增加溶剂产生微生物的溶剂生产。 所述方法包括将感兴趣的微生物工程化以表达异源热休克蛋白/伴侣，例如II型伴侣蛋白或诸如γ-预折蛋白之类的预折合蛋白，其中异源蛋白质来自极端亲本，例如古细菌。
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公开(公告)号：US06579703B2
公开(公告)日：2003-06-17
申请号：US09835909
申请日：2001-04-16
IPC分类号： C12D1934
CPC分类号： C12N9/96 , C07K14/195 , C12Q1/686 , C12Q2527/125
摘要： Small heat shock proteins, e.g., Pyrococcus fuiosus (Pfu-sHSP), confer thermotolerance on cellular cultures and on proteins in cellular extracts during prolonged incubation at elevated temperature, demonstrating the ability to protect cellular proteins and maintain cellular viability under heat stress conditions. Such heat shock proteins are effective to combat enzymatic aggregation and intracellular precipitation during heat stress, and thereby enable enhancement of the utility and stability of enzymes in various applications, e.g., Taq polymerase in PCR applications, digestive enzymes in microbial degradative applications, etc.
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3.发明授权Heterologous expression of extremophile heat shock proteins and chaperones in microorganisms to increase tolerance to toxic compounds 有权极微热热休克蛋白和分子伴侣在微生物中的异源表达，以增加对有毒化合物的耐受性公开(公告)号：US08685729B2
公开(公告)日：2014-04-01
申请号：US12528620
申请日：2008-02-26
IPC分类号： C12N5/00 , C12N1/00 , C12N1/20 , C12N15/74 , C12Q1/02 , C12P21/06 , C12P7/06 , C12P7/16 , C07K1/00 , C07H21/04
CPC分类号： C07K14/195 , Y02E50/17
摘要： The invention provides methods and compositions for increasing tolerance of microorganisms to toxic agents, such as solvents; and for increasing production of solvents from solvent-generating microorganisms. The methods comprise engineering a microorganism of interest to express a heterologous heat-shock protein/chaperone, e.g., Group II chaperonin or a prefoldin such as γ-prefoldin, where the heterologous protein is from an extremophile, such as an archaean.
摘要翻译： 本发明提供了增加微生物对毒性剂如溶剂的耐受性的方法和组合物; 并用于增加溶剂产生微生物的溶剂生产。 所述方法包括将感兴趣的微生物工程化以表达异源热休克蛋白/伴侣，例如II型伴侣蛋白或诸如γ-预折蛋白之类的预折合蛋白，其中异源蛋白质来自极端亲本，例如古细菌。
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公开(公告)号：US07247432B2
公开(公告)日：2007-07-24
申请号：US10390292
申请日：2003-03-17
IPC分类号： C12Q1/68
CPC分类号： C07K14/195 , C12N9/96 , C12Q1/686 , C12Q2527/125
摘要： Small heat shock proteins, e.g., Pyrococcus fuiosus (Pfu-sHSP and/or Pfu-tsHSP), confer thermotolerance on cellular cultures and on proteins in cellular extracts during prolonged incubation at elevated temperature, demonstrating the ability to protect cellular proteins and maintain cellular viability under heat stress conditions. Such heat shock proteins are effective to combat enzymatic aggregation and intracellular precipitation during heat stress, and thereby enable enhancement of the utility and stability of enzymes in various applications, e.g., Taq polymerase in PCR applications, digestive enzymes in microbial degradative applications, etc.
摘要翻译： 小热休克蛋白，例如热球菌（Pfu-sHSP和/或Pfu-tsHSP），在高温下长时间孵育时赋予细胞培养物上的耐热性和细胞提取物中的蛋白质，证明保护细胞蛋白质并保持细胞活力的能力 在热应力条件下。 这种热休克蛋白在热胁迫期间有效地抵抗酶聚集和细胞内沉淀，从而能够增强酶在各种应用中的效用和稳定性，例如PCR应用中的Taq聚合酶，微生物降解应用中的消化酶等。
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