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公开(公告)号：US20080085835A1
公开(公告)日：2008-04-10
申请号：US11537495
申请日：2006-09-29
申请人： Frederick M. AUSUBEL , Brian J. Staskawicz , Andrew F. Bent , Douglas Dahlbeck , Fumiaki Katagiri , Barbara N. Kunkel , Michael Nicholas , Guo-Liang Yu , Barbara Baker , Jeffrey Ellis , John Salmeron
发明人： Frederick M. AUSUBEL , Brian J. Staskawicz , Andrew F. Bent , Douglas Dahlbeck , Fumiaki Katagiri , Barbara N. Kunkel , Michael Nicholas , Guo-Liang Yu , Barbara Baker , Jeffrey Ellis , John Salmeron
CPC分类号： C12Q1/6895 , C07K14/415 , C12N15/8281 , C12Q2600/13 , C12Q2600/156
摘要： Disclosed is substantially pure DNA encoding an Arabidopsis thaliana Rps2 polypeptide, substantially pure Rps2 polyneptide; and methods of using such DNA to express the Rps2 polypeptide in plant cells and whole plants to provide, in transgenic plants, disease resistance to pathogens. Also disclosed are conserved regions characteristic of the RPS family and primers and probes for the identification and isolation of additional RPS disease-resistance genes.
摘要翻译： 公开了编码拟南芥Rps 2多肽，基本上纯的Rps 2多肽的基本上纯的DNA; 以及使用这些DNA在植物细胞和整个植物中表达Rps2多肽的方法，以在转基因植物中提供对病原体的抗病性。 还公开了用于鉴定和分离另外的RPS抗病基因的RPS家族特征的保守区和引物和探针。
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公开(公告)号：US07179601B2
公开(公告)日：2007-02-20
申请号：US10613472
申请日：2003-07-02
申请人： Frederick M. Ausubel , Brian J. Staskawicz , Andrew F. Bent , Douglas Dahlbeck , Fumiaki Katagiri , Barbara N. Kunkel , Michael Nicholas Mindrinos , Guo-Liang Yu , Barbara Baker , Jeffrey Ellis , John Salmeron
发明人： Frederick M. Ausubel , Brian J. Staskawicz , Andrew F. Bent , Douglas Dahlbeck , Fumiaki Katagiri , Barbara N. Kunkel , Michael Nicholas Mindrinos , Guo-Liang Yu , Barbara Baker , Jeffrey Ellis , John Salmeron
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6895 , C07K14/415 , C12N15/8281 , C12Q2600/13 , C12Q2600/156
摘要： The invention relates to a method of identifying a plant disease-resistance gene that includes the steps of (a) providing a plant tissue sample; (b)introducing by biolistic transformation into the plant tissue sample a candidate plant disease-resistance gene; (c) expressing the candidate plant disease-resistance gene within the plant tissue sample; and (d) determining whether the plant tissue sample exhibits a disease-resistance response, whereby a response identifies a plant disease-resistance gene.
摘要翻译： 本发明涉及鉴定植物病害抗性基因的方法，其包括以下步骤：（a）提供植物组织样品; （b）通过生物弹性转化将候选植物抗病基因引入植物组织样品中; （c）在植物组织样品内表达候选植物病害抗性基因; 和（d）确定植物组织样品是否表现出抗病性反应，由此响应识别植物病害抗性基因。
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公开(公告)号：US06262248B1
公开(公告)日：2001-07-17
申请号：US09301085
申请日：1999-04-28
申请人： Frederick M. Ausubel , Brian J. Staskawicz , Andrew F. Bent , Douglas Dahlbeck , Fumiaki Katagiri , Barbara N. Kunkel , Michael Nicholas Mindrinos , Guo-Liang Yu , Barbara Baker , Jeffrey Ellis , John Salmeron
发明人： Frederick M. Ausubel , Brian J. Staskawicz , Andrew F. Bent , Douglas Dahlbeck , Fumiaki Katagiri , Barbara N. Kunkel , Michael Nicholas Mindrinos , Guo-Liang Yu , Barbara Baker , Jeffrey Ellis , John Salmeron
IPC分类号： C07A2104
CPC分类号： C12Q1/6895 , C07K14/415 , C12N15/8281 , C12Q2600/13 , C12Q2600/156
摘要： Disclosed is substantially pure DNA encoding an Arabidopsis thaliana Rps2 polypeptide; substantially pure Rps2 polypeptide; and methods of using such DNA to express the Rps2 polypeptide in plant cells and whole plants to provide, in transgenic plants, disease resistance to pathogens. Also disclosed are conserved regions characteristic of the RPS family and primers and probes for the identification and isolation of additional RPS disease-resistance genes.
摘要翻译： 公开了编码拟南芥Rps2多肽的基本上纯的DNA; 基本上纯的Rps2多肽; 以及使用这些DNA在植物细胞和整个植物中表达Rps2多肽的方法，以在转基因植物中提供对病原体的抗病性。 还公开了用于鉴定和分离另外的RPS抗病基因的RPS家族特征的保守区和引物和探针。
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4.发明授权Plant resistance gene family encoding resistance polypeptides having P-loop and LRR motifs 失效编码具有P环和LRR基序的抗性多肽的植物抗性基因家族
公开(公告)号：US6127607A
公开(公告)日：2000-10-03
申请号：US841089
申请日：1997-04-29
申请人： Frederick Michael Ausubel , Brian J. Staskawicz , Andrew F. Bent , Douglas Dahlbeck , Fumiaki Katagiri , Barbara N. Kunkel , Michael Nicholas Mindrinos , Guo-Liang Yu
发明人： Frederick Michael Ausubel , Brian J. Staskawicz , Andrew F. Bent , Douglas Dahlbeck , Fumiaki Katagiri , Barbara N. Kunkel , Michael Nicholas Mindrinos , Guo-Liang Yu
IPC分类号： A01H1/00 , A01H5/00 , C07K14/415 , C07K16/16 , C12N5/10 , C12N15/09 , C12N15/82 , C12P21/02 , C12Q1/68 , A01H5/10 , C12N1/21 , C12N5/14
CPC分类号： C12Q1/6895 , C07K14/415 , C12N15/8281 , C12Q2600/13 , C12Q2600/156
摘要： Disclosed is a new plant resistance gene family, the members of which encode plant resistance polypeptides having P-loop and LRR structural motifs. Also disclosed are substantially pure plant DNAs encoding such polypeptides. The invention further involves transgenic plants and transformed host cells that express these DNAs and exhibit enhanced disease resistance to plant pathogens.
摘要翻译： 公开了一种新的植物抗性基因家族，其成员编码具有P环和LRR结构基序的植物抗性多肽。 还公开了基本上纯的编码这种多肽的植物DNA。 本发明还涉及转基因植物和表达这些DNA的转化宿主细胞，并表现出对植物病原体的增强的抗病性。
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公开(公告)号：US5981730A
公开(公告)日：1999-11-09
申请号：US310912
申请日：1994-09-22
申请人： Frederick M. Ausubel , Brian J. Staskawicz , Fumiaki Katagiri , Barbara Baker , Jeffrey Ellis , John Salmeron
发明人： Frederick M. Ausubel , Brian J. Staskawicz , Fumiaki Katagiri , Barbara Baker , Jeffrey Ellis , John Salmeron
IPC分类号： A01H1/00 , A01H5/00 , C07K14/415 , C07K16/16 , C12N5/10 , C12N15/09 , C12N15/82 , C12P21/02 , C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6895 , C07K14/415 , C12N15/8281 , C12Q2600/13 , C12Q2600/156
摘要： Disclosed is substantially pure DNA encoding an Arabidopsis thaliana Rps2 polypeptide; substantially pure Rps2 polypeptide; and methods of using such DNA to express the Rps2 polypeptide in plant cells and whole plants to provide, in transgenic plants, disease resistance to pathogens. Also disclosed are conserved regions characteristic of the RPS family and primers and probes for the identification and isolation of additional RPS disease-resistance genes.
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公开(公告)号：US06262343B1
公开(公告)日：2001-07-17
申请号：US09360186
申请日：1999-07-23
IPC分类号： C12N504
CPC分类号： C07K14/415 , C12N15/8281
摘要： Nucleic acid molecules (cDNAs and genes) that confer resistance to the plant pathogen Xanthomonas campestris are disclosed. These molecules may be introduced into plants that are otherwise susceptible to infection by this bacterium in order to enhance resistance.
摘要翻译： 公开了赋予植物病原体野油菜黄单胞菌耐药性的核酸分子（cDNA和基因）。 这些分子可以被引入到另外对这种细菌感染的植物中以提高抗性。
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公开(公告)号：US5674701A
公开(公告)日：1997-10-07
申请号：US171207
申请日：1993-12-21
IPC分类号： A01H1/04 , C07K14/415 , C12N5/04 , C12N15/82 , G01N33/50 , G01N33/569 , C12Q1/18 , A01H5/00
CPC分类号： G01N33/56961 , A01H1/04 , C07K14/415 , C12N15/8249 , C12N15/8281 , C12N5/04 , G01N33/5097
摘要： A process for identifying a plant having disease tolerance comprising administering to a plant an inhibitory amount of ethylene and screening for ethylene insensitivity, thereby identifying a disease tolerant plant, is described. Plants identified by the foregoing process are also described.
摘要翻译： 用于鉴定具有疾病耐受性的植物的方法包括向植物施用抑制量的乙烯并筛选乙烯不敏感性，从而鉴定耐病植物。 还描述了通过前述方法鉴定的植物。
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公开(公告)号：US20140137292A1
公开(公告)日：2014-05-15
申请号：US13976703
申请日：2012-01-12
IPC分类号： C12N15/82
CPC分类号： C12N15/8216 , C07K14/415 , C12N15/8238 , C12N15/8239 , C12N15/8263 , C12N15/8281
摘要： Methods and compositions for making citrus plants with enhanced resistance to Asiatic citrus canker (ACC) and other forms of citrus canker caused by Xanthomonas are provided. The methods involve transforming citrus plant cells with polynucleotide constructs comprising a promoter operably linked to nucleotide sequence that encodes a protein that is capable of triggering cell death in a citrus plant. The promoters of the invention are inducible by one or more Xanthomonas strains that cause citrus canker. Isolated nucleic acid molecules and expression cassettes comprising such polynucleotide constructs and promoters are further provided. Citrus plants with enhanced resistance to citrus canker are also provided.
摘要翻译： 提供了用于制备具有对亚洲柑橘溃疡（ACC）和由黄单胞菌属引起的其他形式的柑橘溃疡具有增强抗性的柑橘植物的方法和组合物。 所述方法包括用包含可操作地连接到核苷酸序列的多核苷酸构建体来转化柑橘类植物细胞，核苷酸序列编码能够引发柑橘植物中的细胞死亡的蛋白质。 本发明的启动子可诱导一种或多种引起柑橘溃疡的黄单孢菌属菌株。 还提供了分离的核酸分子和包含这种多核苷酸构建体和启动子的表达盒。 还提供了具有增强抗柑橘油菜的柑橘类植物。
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公开(公告)号：US06762285B2
公开(公告)日：2004-07-13
申请号：US09864680
申请日：2001-05-24
IPC分类号： C07K14415
CPC分类号： C07K14/415 , C12N15/8281
摘要： Bs2 resistance proteins that confer resistance to the plant pathogen Xanthomonas campestris are disclosed. These proteins may be expressed in transgenic plants that are otherwise susceptible to infection by this bacterium in order to enhance resistance.
摘要翻译： 公开了赋予植物病原体野油菜黄单胞菌耐药性的Bs2抗性蛋白质。 这些蛋白质可以在转基因植物中表达，否则其对该细菌的感染易感，以增强抗性。
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公开(公告)号：US4464473A
公开(公告)日：1984-08-07
申请号：US371162
申请日：1982-04-23
IPC分类号： C07H21/04 , C07K14/21 , C07K14/27 , C12N20060101 , C12N1/00 , C12N1/20 , C12N5/00 , C12N15/00 , C12N15/31 , C12N15/70 , F25C1/00 , F25C3/04 , C12P21/00
摘要： DNA sequences encoding for ice nucleation activity are isolated and introduced into unicellular hosts. The modified hosts demonstrate ice nucleation activity analogous to the DNA source host. The cellular products find use in inhibiting supercooling.
摘要翻译： 分离编码冰核活性的DNA序列并将其引入单细胞宿主体内。 修饰的宿主证明类似于DNA源宿主的冰核活动。 蜂窝产品用于抑制过冷。
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