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    Methods for screening for gene specific hybridization polymorphisms (GSHPs) and their use in genetic mapping ane marker development
    5.
    发明申请
    Methods for screening for gene specific hybridization polymorphisms (GSHPs) and their use in genetic mapping ane marker development 审中-公开
    筛选基因特异性杂交多态性（GSHPs）的方法及其在遗传图谱分析中的应用

    公开(公告)号：US20070192909A1

    公开(公告)日：2007-08-16

    申请号：US11725636

    申请日：2007-03-20

    申请人： John Salmeron ,  Tong Zhu

    发明人： John Salmeron ,  Tong Zhu

    IPC分类号： A01H5/00 ,  C12Q1/68 ,  G06F19/00

    CPC分类号： C12Q1/6827 ,  G16B25/00

    摘要： A method for identification of gene specific hybridization polymorphisms (GSHPs) and their use is presented. The method involves the steps of a) global screening for hybridization polymorphisms using microarray; b) enzyme mediated genome complexity reduction; c) enzyme mediated differential signal amplification and noise reduction; d) data extraction and GSHP identification; and e) use of GSHPs in high throughput screening. The method is useful in a particular application for the fingerprinting of maize exotic germplasm and the identification of new and commercially useful loci therein.

    摘要翻译： 提出了一种鉴定基因特异性杂交多态性（GSHPs）及其应用的方法。 该方法包括以下步骤：a）使用微阵列全面筛选杂交多态性; b）酶介导的基因组复杂性降低; c）酶介导的差异信号放大和降噪; d）数据提取和GSHP鉴定; 和e）在高通量筛选中使用GSHPs。 该方法在特定应用中用于玉米异种种质的指纹识别和其中新的和商业上有用的基因座的鉴定。

    Transgenic plants compromising nucleic acid molecules encoding RAR1 disease resistance proteins and uses thereof
    7.
    发明授权
    Transgenic plants compromising nucleic acid molecules encoding RAR1 disease resistance proteins and uses thereof 失效
    转基因植物损害编码RAR1抗病性蛋白质的核酸分子及其用途

    公开(公告)号：US07098378B2

    公开(公告)日：2006-08-29

    申请号：US11011906

    申请日：2004-12-14

    申请人： Manuel B. Sainz ,  John Salmeron

    发明人： Manuel B. Sainz ,  John Salmeron

    IPC分类号： C12N15/09 ,  C12N15/29 ,  C12N15/82 ,  A01H5/00 ,  A01H5/10

    CPC分类号： C12N15/8281 ,  C07K14/415 ,  C12N15/8279 ,  C12N15/8282

    摘要： The present invention pertains to transgenic plants comprising nucleic acid molecules comprising nucleotide sequences that encode RAR1 proteins involved in disease resistance, and the RAR1 polypeptides. The invention particularly relates to methods of altering the expressing nucleic acid molecules encoding RAR1 proteins in transgenic plants to alter the level disease resistance, and to transgenic plants, progeny and seed therefrom, having altered enhanced disease resistance. The invention further relates to methods of enhancing expression of R resistance genes, disease resistance signal transduction genes, genes involved in mediating disease resistance or involved in the synthesis of molecules mediating disease resistance. The invention also relates to methods of regulating the expression of other coding sequences of interest by increasing the expression of the nucleic acid molecules of the invention.

    摘要翻译： 本发明涉及包含核酸分子的转基因植物，所述核酸分子包含编码参与抗病性的RAR1蛋白的核苷酸序列和RAR1多肽。 本发明特别涉及改变转基因植物中编码RAR1蛋白的表达核酸分子以改变水平的抗病性的方法，以及改变增强的抗病性的转基因植物，后代和种子。 本发明还涉及增强R抗性基因表达，抗病性信号转导基因，介导抗病性的基因或参与抗病性分子合成的方法。 本发明还涉及通过增加本发明的核酸分子的表达来调节其它编码序列的表达的方法。

    Trichothecene-resistant transgenic plants
    9.
    发明授权
    Trichothecene-resistant transgenic plants 有权
    抗逆转录病毒转基因植物

    公开(公告)号：US06646184B2

    公开(公告)日：2003-11-11

    申请号：US10074279

    申请日：2002-02-12

    申请人： Thomas M. Hohn ,  Cheryl Peters ,  John Salmeron

    发明人： Thomas M. Hohn ,  Cheryl Peters ,  John Salmeron

    IPC分类号： C12N1509

    CPC分类号： C07H21/04 ,  C12N9/1029 ,  C12N15/8209 ,  C12N15/8242 ,  C12N15/8274 ,  C12N15/8282

    摘要： The present invention discloses trichothecene-resistant transgenic plants, plant tissues, plant seeds, and plant cells comprising a heterologous polynucleotide encoding a gene product having tricothecene resistance activity that thereby confers trichothecene resistance to the transgenic plants, plant tissues, plant seeds, and plant cells. Trichothecene resistance activity, as used herein, refers to an activity that reduces or inhibits the phytotoxicity of a trichothecene, particularly to a fungus and/or plant. In a particular embodiment, trichothecene resistance activity refers to an activity that transfers an acetate to the C-3 position of a trichothecene such as T-2 toxin, HT-2 toxin, isotrichodermol, diacetoxyscirpenol (“DAS”), 3-deacetylcalonectrin, 3,15-dideacetylcalonectrin, scirpentriol, neosolaniol; 15-acetyldeoxynivalenol, nivalenol, 4-acetylnivalenol (fusarenone-X), 4,15-diacetylnivalenol, 4,7,15-acetylnivalenol, and deoxynivalenol (“DON”) and their various acetylated derivatives. In another particular embodiment, the gene product having trichthecene resistance activity is a 3-O-acetyltransferase from a trichothecene-producing species of Fusarium, such as Fusarium graminearum or Fusarium sporotrichioides.

    摘要翻译： 本发明公开了头孢霉烯抗性转基因植物，植物组织，植物种子和植物细胞，其包含编码具有三氯生物抗性活性的基因产物的异源多核苷酸，从而赋予转基因植物，植物组织，植物种子和植物细胞的单端孢霉烯抗性 。 如本文所用，三氯乙炔抗性活性是指减少或抑制单端孢霉烯特别是真菌和/或植物的植物毒性的活性。 在一个特定的实施方案中，单端孢霉烯抗性活性是指将乙酸酯转移到单端孢霉烯C-3位的活性，例如T-2毒素，HT-2毒素，异维甲酸，二乙酰氧基甲苯酚（“DAS”），3-脱乙酰基木聚糖， 3,15-二乙酰胆碱，顺丁三醇，新溶胶甘醇; 15-乙酰基过氧化雪腐镰刀菌烯醇，新戊烯醇，4-乙酰基雪腐镰刀菌烯醇（fisarenone-X），4,15-二乙酰基雪腐镰刀菌烯醇，4,7,15-乙酰基雪腐镰刀菌烯醇和脱氧雪腐镰刀菌烯醇（“DON”）及其各种乙酰化衍生物。 在另一个具体实施方案中，具有三辛抗性活性的基因产物是来自产生单端孢霉烯的镰刀菌属物种如禾谷镰刀菌（Fusarium graminearum）或孢子镰刀菌（Fusarium sporotrichioides）的3-O-乙酰转移酶。

    Transgenic plants compromising nucleic acid molecules encoding RAR 1 disease resistance proteins and uses thereof
    10.
    发明申请
    Transgenic plants compromising nucleic acid molecules encoding RAR 1 disease resistance proteins and uses thereof 审中-公开
    转基因植物损害编码RAR1抗病性蛋白质的核酸分子及其用途

    公开(公告)号：US20050097637A1

    公开(公告)日：2005-05-05

    申请号：US11012974

    申请日：2004-12-15

    申请人： Manuel Sainz ,  John Salmeron

    发明人： Manuel Sainz ,  John Salmeron

    IPC分类号： C07K14/415 ,  C12N15/82 ,  A01H1/00

    CPC分类号： C12N15/8281 ,  C07K14/415 ,  C12N15/8279 ,  C12N15/8282

    摘要： The present invention pertains to transgenic plants comprising nucleic acid molecules comprising nucleotide sequences that encode RAR1 proteins involved in disease resistance, and the RAR1 polypeptides. The invention particularly relates to methods of altering the expressing nucleic acid molecules encoding RAR1 proteins in transgenic plants to alter the level disease resistance, and to transgenic plants, progeny and seed therefrom, having altered enhanced disease resistance. The invention further relates to methods of enhancing expression of R resistance genes, disease resistance signal transduction genes, genes involved in mediating disease resistance or involved in the synthesis of molecules mediating disease resistance. The invention also relates to methods of regulating the expression of other coding sequences of interest by increasing the expression of the nucleic acid molecules of the invention.

    摘要翻译： 本发明涉及包含核酸分子的转基因植物，所述核酸分子包含编码参与抗病性的RAR1蛋白的核苷酸序列和RAR1多肽。 本发明特别涉及改变转基因植物中编码RAR1蛋白的表达核酸分子以改变水平的抗病性的方法，以及改变增强的抗病性的转基因植物，后代和种子。 本发明还涉及增强R抗性基因表达，抗病性信号转导基因，介导抗病性的基因或参与抗病性分子合成的方法。 本发明还涉及通过增加本发明的核酸分子的表达来调节其它编码序列的表达的方法。