公开(公告)号：US20190217552A1

公开(公告)日：2019-07-18

申请号：US16179791

申请日：2018-11-02

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Randy Davis

IPC: B29C67/20 ,  C12Q1/6869 ,  B82Y5/00 ,  G01N33/487

CPC classification number: B29C67/20 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y40/00 ,  C12Q1/6869 ,  G01N33/48721 ,  Y10S977/797 ,  Y10S977/90 ,  Y10S977/942 ,  C12Q2565/631

Abstract: Techniques for forming a nanopore in a lipid bilayer are described herein. In one example, an agitation stimulus level such as an electrical agitation stimulus is applied to a lipid bilayer wherein the agitation stimulus level tends to facilitate the formation of nanopores in the lipid bilayer. In some embodiments, a change in an electrical property of the lipid bilayer resulting from the formation of the nanopore in the lipid bilayer is detected, and a nanopore has formed in the lipid bilayer is determined based on the detected change in the lipid bilayer electrical property.

公开(公告)号：US20190064141A1

公开(公告)日：2019-02-28

申请号：US16172633

申请日：2018-10-26

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Hui Tian ,  Santiago Fernandez-Gomez

IPC: G01N33/487 ,  C12Q1/6869 ,  B82Y15/00

CPC classification number: G01N33/48721 ,  B82Y15/00 ,  C12Q1/6869

Abstract: A method of exporting measurements of a nanopore sensor on a nanopore based sequencing chip is disclosed. An electrical characteristic associated with the nanopore sensor is measured. The electrical characteristic associated with the nanopore sensor is processed. A summary for the electrical characteristic and one or more previous electrical characteristics is determined. The summary for the electrical characteristic and the one or more previous electrical characteristics are exported. Determining the summary includes determining that the electrical characteristic and at least a portion of the one or more previous electrical characteristics correspond to a base call event at the nanopore sensor. The summary represents the electrical characteristic and the at least a portion of the one or more previous electrical characteristics.

公开(公告)号：US20170361298A1

公开(公告)日：2017-12-21

申请号：US15697904

申请日：2017-09-07

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen

IPC: B01J19/00 ,  G05D23/20

CPC classification number: B01J19/0046 ,  B01J2219/00495 ,  B01J2219/00698 ,  G05D23/1919 ,  G05D23/2034 ,  G05D23/2037

Abstract: A system for regulating a temperature of a measurement array is disclosed. The system includes a measurement array including a plurality of sensors, wherein the plurality of sensors are integrated onto an integrated circuit die. The system includes a thermal sensor integrated onto the integrated circuit die, wherein the thermal sensor senses a temperature associated with the plurality of sensors. The system further includes a heat pump coupled to the integrated circuit die, wherein the heat pump is controlled by a feedback control circuit including the thermal sensor.

公开(公告)号：US20170283867A1

公开(公告)日：2017-10-05

申请号：US15085713

申请日：2016-03-30

Applicant: Genia Technologies, Inc.

Inventor： Ashraf Wahba ,  William Nielsen ,  J. William Maney, JR. ,  Kevin Aliado ,  Kyle Umeda ,  Roger J.A. Chen ,  Jing Luo

IPC: C12Q1/68

Abstract: A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A salt buffer solution is flowed over the array of cells in the nanopore based sequencing chip to substantially fill the wells in the cells with the salt buffer solution. A lipid and solvent mixture is flowed over the array of cells to deposit the lipid and solvent mixture over at least some of the wells in the cells. A first portion of the cells, each having a lipid bilayer over its well, is detected. A second portion of the cells, each having a lipid membrane but not a lipid bilayer over its well, is detected. An electrical lipid-thinning stimulus is selectively applied to the second portion of the cells but not to the first portion of the cells.

公开(公告)号：US20170283866A1

公开(公告)日：2017-10-05

申请号：US15085700

申请日：2016-03-30

Applicant: Genia Technologies, Inc.

Inventor： Ashraf Wahba ,  William Nielsen ,  Jing Luo ,  Kevin Aliado ,  Kyle Umeda ,  Roger J.A. Chen

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  G01N33/48721 ,  C12Q2565/607

Abstract: A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.

公开(公告)号：US20170103952A1

公开(公告)日：2017-04-13

申请号：US15240872

申请日：2016-08-18

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen

IPC: H01L23/552 ,  G01N33/487 ,  G01N27/447 ,  H01L23/522 ,  H01L23/58

CPC classification number: H01L23/552 ,  B82Y15/00 ,  G01N27/44756 ,  G01N27/44791 ,  G01N33/48721 ,  H01L21/76877 ,  H01L23/5225 ,  H01L23/585 ,  H01L28/60 ,  H01L2924/0002 ,  H01L2924/00

Abstract: A device having an integrated noise shield is disclosed. The device includes a plurality of vertical shielding structures substantially surrounding a semiconductor device. The device further includes an opening above the semiconductor device substantially filled with a conductive fluid, wherein the plurality of vertical shielding structures and the conductive fluid shield the semiconductor device from ambient radiation. In some embodiments, the device further includes a conductive bottom shield below the semiconductor device shielding the semiconductor device from ambient radiation. In some embodiments, the opening is configured to allow a biological sample to be introduced into the semiconductor device. In some embodiments, the vertical shielding structures comprise a plurality of vias, wherein each of the plurality of vias connects more than one conductive layers together. In some embodiments, the device comprises a nanopore device, and wherein the nanopore device comprises a single cell of a nanopore array.

公开(公告)号：US20150185185A1

公开(公告)日：2015-07-02

申请号：US14558222

申请日：2014-12-02

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen

IPC: G01N27/447 ,  H01L21/768

CPC classification number: H01L23/552 ,  B82Y15/00 ,  G01N27/44756 ,  G01N27/44791 ,  G01N33/48721 ,  H01L21/76877 ,  H01L23/5225 ,  H01L23/585 ,  H01L28/60 ,  H01L2924/0002 ,  H01L2924/00

Abstract: A device having an integrated noise shield is disclosed. The device includes a plurality of vertical shielding structures substantially surrounding a semiconductor device. The device further includes an opening above the semiconductor device substantially filled with a conductive fluid, wherein the plurality of vertical shielding structures and the conductive fluid shield the semiconductor device from ambient radiation. In some embodiments, the device further includes a conductive bottom shield below the semiconductor device shielding the semiconductor device from ambient radiation. In some embodiments, the opening is configured to allow a biological sample to be introduced into the semiconductor device. In some embodiments, the vertical shielding structures comprise a plurality of vias, wherein each of the plurality of vias connects more than one conductive layers together. In some embodiments, the device comprises a nanopore device, and wherein the nanopore device comprises a single cell of a nanopore array.

Abstract translation: 公开了一种具有集成噪声屏蔽的装置。 该装置包括基本上围绕半导体器件的多个垂直屏蔽结构。 该器件还包括在基本上填充有导电流体的半导体器件上方的开口，其中多个垂直屏蔽结构和导电流体屏蔽半导体器件免受环境辐射。 在一些实施例中，该器件还包括在半导体器件下方的导电底部屏蔽件，以遮蔽半导体器件免受环境辐射。 在一些实施例中，开口被配置为允许生物样品被引入到半导体器件中。 在一些实施例中，垂直屏蔽结构包括多个通孔，其中多个通孔中的每一个将不止一个导电层连接在一起。 在一些实施方案中，所述装置包括纳米孔装置，并且其中所述纳米孔装置包括纳米孔阵列的单个细胞。

公开(公告)号：US20150056364A1

公开(公告)日：2015-02-26

申请号：US14334523

申请日：2014-07-17

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Randy Davis

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  B82Y5/00 ,  G01N33/48721

Abstract: Techniques for assembling a lipid bilayer on a substantially planar solid surface are described herein. In one example, a lipid material such as a lipid suspension is deposited on a substantially planar solid surface, a bubble filled with fast diffusing gas molecules is formed on the solid surface, and the gas molecules are allowed to diffuse out of the bubble to form a lipid bilayer on the solid surface.

Abstract translation: 本文描述了在基本上平面的固体表面上组装脂质双层的技术。 在一个实例中，脂质材料如脂质悬浮液沉积在基本上平面的固体表面上，在固体表面上形成填充有快速扩散气体分子的气泡，并使气体分子扩散出气泡形成 固体表面上的脂质双层。

公开(公告)号：US20140203464A1

公开(公告)日：2014-07-24

申请号：US14150322

申请日：2014-01-08

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Randy Davis

IPC: C12Q1/68

CPC classification number: G01N33/48721 ,  B81B1/00 ,  B82Y5/00 ,  G01N15/12

Abstract: A method of forming a nanopore in a lipid bilayer is disclosed. A nanopore forming solution is deposited over a lipid bilayer. The nanopore forming solution has a concentration level and a corresponding activity level of pore molecules such that nanopores are substantially not formed un-stimulated in the lipid bilayer. Formation of a nanopore in the lipid bilayer is initiated by applying an agitation stimulus level to the lipid bilayer. In some embodiments, the concentration level and the corresponding activity level of pore molecules are at levels such that less than 30 percent of a plurality of available lipid bilayers have nanopores formed un-stimulated therein.

Abstract translation: 公开了一种在脂质双层中形成纳米孔的方法。 将纳米孔形成溶液沉积在脂质双层上。 形成纳米孔的溶液具有孔分子的浓度水平和相应的活性水平，使得纳米孔在脂双层中基本上未形成未刺激。 脂质双层中的纳米孔的形成是通过向脂质双层施加搅拌刺激水平来引发的。 在一些实施方案中，孔分子的浓度水平和相应的活性水平的水平使得少于30％的多个可用脂质双层具有在其中未被刺激的纳米孔。

公开(公告)号：US20190004028A1

公开(公告)日：2019-01-03

申请号：US16021664

申请日：2018-06-28

Applicant: Genia Technologies, Inc.

Inventor： Jennifer Hovis ,  Hui Tian ,  Roger J.A. Chen

IPC: G01N33/487

CPC classification number: G01N33/48721

Abstract: A nanopore sequencing device is disclosed. The nanopore sequencing device includes a working electrode. It further includes a dielectric layer, wherein a portion of the dielectric layer is disposed horizontally adjacent to the working electrode and a portion of the dielectric layer is disposed above and covering a portion of the working electrode, and wherein the dielectric layer forms a well having an opening above an uncovered portion of the working electrode. A base surface area of the working electrode is greater than a base surface area of the opening above the uncovered portion of the working electrode.