公开(公告)号：US20240240160A1

公开(公告)日：2024-07-18

申请号：US18564967

申请日：2022-05-27

Applicant: GENOMATICA, INC.

Inventor： Andreas W. SCHIRMER ,  Michael Angus NOBLE ,  Jamison Parker HUDDLESTON

IPC: C12N9/10 ,  C12N15/52 ,  C12N15/70 ,  C12P7/22 ,  C12P7/42 ,  C12P17/06 ,  C12P19/32

CPC classification number: C12N9/1029 ,  C12N15/52 ,  C12N15/70 ,  C12P7/22 ,  C12P7/42 ,  C12P17/06 ,  C12P19/32 ,  C12Y203/01206

Abstract: The present disclosure provides a poly nucleotide comprising: (a) a nucleic acid sequence encoding an olivetol synthase (OLS): and (b) a heterologous regulatory element operably linked to the nucleic acid sequence. The present disclosure further relates to an engineered cell comprising an olivetol synthase (OLS). Also provided are a cell extract or cell culture medium or a composition comprising 3.5.7- trioxododecanoyl-CoA, olivetol, olivetolic acid, a cannabinoid, and/or an isomer, analog, or derivative thereof, and a method of making 3.5.7-trioxododecanoyl-CoA, olivetol, olivetolic acid, a cannabinoid, and/or an isomer, analog, or derivative thereof. Also provided is a cannabinoid produced by the engineered cell, isolated from the cell extract or cell culture medium, and/or made by the method described herein. The present disclosure also provides a non-natural olivetol synthase (OLS).