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公开(公告)号：US20240110206A1
公开(公告)日：2024-04-04
申请号：US18031522
申请日：2021-10-12
发明人： Dylan Alexander Carlin , Brian Carvalho , Nicholas Flores , Katrina Forrest , Gabriel Rodriguez , Nathan W. Schmidt , Michelle Spencer
CPC分类号： C12P7/42 , C12N9/1029 , C12N9/1085 , C12N15/62 , C12N15/81 , C12Y203/01206 , C12Y205/0101
摘要： Aspects of the disclosure relate to biosynthesis of cannabinoids and cannabinoid precursors in recombinant cells and in vitro. Specifically, the disclosure is directed to a prenyltransferase variant, a chimeric prenyltransferase comprising one or more portions of at least two different prenyltransferase proteins, and a fusion polypeptide comprising a CBG-type producing prenyltransferase and farnesyl pyrophosphate synthase.
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公开(公告)号：US11884948B2
公开(公告)日：2024-01-30
申请号：US17449847
申请日：2021-10-04
申请人： PYRONE SYSTEMS, INC.
CPC分类号： C12P17/06 , C12N1/16 , C12P7/42 , C12Y103/03006 , C12Y203/01206 , C12Y404/01026
摘要： Strains of yeasts are provided containing the genes for the production of cannabinoids from fatty acids. The enzymes that mediate cannabinoid production are localized to the cytosol, peroxisome or different compartments within the secretory pathway (e.g., endoplasmic reticulum, Golgi, vacuole) to ensure efficient production. The engineered microorganisms produce cannabinoids in a controlled fermentation process.
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公开(公告)号：US20240026392A1
公开(公告)日：2024-01-25
申请号：US18015046
申请日：2021-07-08
发明人： Kim Cecelia Anderson , Jeffrey Ian Boucher , Elena Brevnova , Brian Carvalho , Nicholas Flores , Katrina Forrest , Fiona Qu , Gabriel Rodrigues , Michelle Spencer
CPC分类号： C12P7/42 , C12P7/22 , C12N15/81 , C12Y121/03008 , C12Y404/01026 , C12Y205/0101 , C12Y203/01206 , C12N2800/102
摘要： Aspects of the disclosure relate to biosynthesis of cannabinoids and cannabinoid precursors in recombinant cells and in vitro, and to variant terminal synthases having altered catalytic activity to synthesize THC-type, CBD-type and/or CBC-type cannabinoids.
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公开(公告)号：US20230272439A1
公开(公告)日：2023-08-31
申请号：US18013082
申请日：2021-07-01
申请人： LEIBNIZ-INSTITUT FÜR NATURSTOFF-FORSCHUNG UND INFEKTIONSBIOLOGIE E. V. HANS-KNÖLL-INSTIUT (HKI)
CPC分类号： C12P7/42 , C12N15/52 , C12Y203/01206 , C12N9/1029 , C12Y404/01026 , C12N9/88
摘要： The present invention relates to a method for the recombinant production of olivetolic acid (OA) in a host species selected from amoebozoa, based on a hybrid-gene or enzyme of polyketide synthase 37 (PKS37) in which the C-terminal type III PKS domain from an amoeba is replaced by an olivetol synthase (OLS) from a plant, and is expressed together with an olivetolic acid cyclase from a multi-gene expression vector. Further provided is a recombinant amoebozoa host species, and an improved method for producing Δ9-tetrahydrocannabinol (THC) or other cannabinoids.
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公开(公告)号：US20230265465A1
公开(公告)日：2023-08-24
申请号：US17962229
申请日：2022-10-07
发明人： Jordan Buck
CPC分类号： C12P7/42 , C12N15/8222 , C12N15/70 , C12N15/74 , C12N15/81 , C12N2330/51 , C12Y203/01009 , C12Y121/03008 , C12Y103/03 , C12Y121/03007 , C12Y203/01206 , C12Y404/01026 , C12Y205/01001 , C12Y503/03002 , C12Y101/01157 , C12Y402/01017 , C12Y203/01016 , C12Y103/01038 , C12Y203/0301 , C12Y101/01034 , C12Y207/01036 , C12Y207/04002 , C12Y401/01033 , C12Y203/03008 , C12Y604/01002 , C12N2800/40
摘要： This document relates to using bidirectional, multi-enzymatic scaffolds to biosynthesize cannabinoids in recombinant hosts.
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公开(公告)号：US10059971B2
公开(公告)日：2018-08-28
申请号：US15438291
申请日：2017-02-21
发明人： Jonathan E. Page , Steve Gagne
CPC分类号： C12P17/06 , C12N9/1025 , C12N15/52 , C12N15/70 , C12N15/8243 , C12P7/22 , C12P7/42 , C12Y203/01206 , C12Y404/01026 , Y02P20/52
摘要： Nucleic acid molecules encoding polypeptides having polyketide synthase activity have been identified and characterized. Expression or over-expression of the nucleic acids alters levels of cannabinoid compounds in organisms. The polypeptides may be used in vivo or in vitro to produce cannabinoid compounds.
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公开(公告)号：US20170159080A1
公开(公告)日：2017-06-08
申请号：US15441109
申请日：2017-02-23
CPC分类号： C12P7/26 , A01H6/28 , A61K31/352 , C12M21/18 , C12M41/30 , C12M41/48 , C12N9/0004 , C12N9/1029 , C12P7/04 , C12P7/22 , C12P7/42 , C12P7/62 , C12P17/06 , C12Y121/03007 , C12Y121/03008 , C12Y203/01206 , C12Y205/01102 , Y02A50/473
摘要： The present invention provides methods for producing cannabinoids and cannabinoid analogs as well as a system for producing these compounds. The inventive method is directed to contacting a compound according to Formula I or Formula II with a cannabinoid synthase. Also described is a system for producing cannabinoids and cannabinoid analogs by contacting a THCA synthase with a cannabinoid precursor and modifying at least one property of the reaction mixture to influence the quantity formed of a first cannabinoid relative to the quantity formed of a second cannabinoid.
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公开(公告)号：US20230332193A1
公开(公告)日：2023-10-19
申请号：US18043258
申请日：2021-09-01
申请人： GENOMATICA, INC.
CPC分类号： C12P17/06 , C12N9/0006 , C12N9/1029 , C12Y203/01206 , C12N9/88 , C12Y404/01026 , C12N9/1085 , C12Y205/0101 , C12N15/52
摘要： The disclosure relates to a non-natural flavin-dependent oxidase comprising at least one amino acid variation as compared to a wild type flavin-dependent oxidase, wherein the non-natural flavin-dependent oxidase does not comprise a disulfide bond, and wherein the non-natural flavin-dependent oxidase is capable of oxidative cyclization of a prenylated aromatic compound into a cannabinoid. The disclosure also relates to a nucleic acid, an expression construct, and an engineered cell for making the non-natural flavin-dependent oxidase. Also provided are compositions comprising the non-natural flavin-dependent oxidase; isolated non-natural flavin-dependent oxidase and methods of making the same; cell extracts comprising the non-natural flavin-dependent oxidase; and methods of making cannabinoids.
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9.发明公开公开(公告)号：US20230279452A1
公开(公告)日：2023-09-07
申请号：US17799414
申请日：2021-02-09
申请人： LYGOS, INC.
发明人： NICHOLAS OHLER , JASON POULOS , CHI LE , NEIL MCALPINE , ANTHONY FARINA
CPC分类号： C12P17/06 , C12N15/52 , C12P7/22 , C12Y203/01206 , C12Y404/01026 , C12Y602/01002
摘要： Provided herein are processes, including semi-synthetic, and synthetic processes for preparing cannabinoids, and cannabinoid compositions provided thereby.
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公开(公告)号：US10081818B2
公开(公告)日：2018-09-25
申请号：US15430122
申请日：2017-02-10
CPC分类号： C12P7/26 , A01H6/28 , A61K31/352 , C12M21/18 , C12M41/30 , C12M41/48 , C12N9/0004 , C12N9/1029 , C12P7/04 , C12P7/22 , C12P7/42 , C12P7/62 , C12P17/06 , C12Y121/03007 , C12Y121/03008 , C12Y203/01206 , C12Y205/01102 , Y02A50/473
摘要： The present invention provides methods for producing cannabinoids and cannabinoid analogs as well as a system for producing these compounds. The inventive method is directed to contacting a compound according to Formula I or Formula II with a cannabinoid synthase. Also described is a system for producing cannabinoids and cannabinoid analogs by contacting a THCA synthase with a cannabinoid precursor and modifying at least one property of the reaction mixture to influence the quantity formed of a first cannabinoid relative to the quantity formed of a second cannabinoid.
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