公开(公告)号：US10941454B2

公开(公告)日：2021-03-09

申请号：US15578488

申请日：2016-05-26

Applicant: GENOMATICA, INC. ,  BRASKEM S.A.

Inventor： Stephanie J. Culler ,  Robert J. Haselbeck ,  Harish Nagarajan ,  Iuri Estrada Gouvea ,  Daniel Johannes Koch ,  Mateus Schreiner Garcez Lopes ,  Lucas Pedersen Parizzi

IPC: C12N9/88 ,  C12N9/90 ,  C07K16/40 ,  C08F36/06 ,  C12N15/82 ,  C12P5/02 ,  C12P7/16

Abstract: In alternative embodiments, provided are non-natural or genetically engineered vinylisomerase-dehydratase enzymes, including alkenol dehydratases, linalool dehydratases and crotyl alcohol dehydratases. In alternative embodiments, provided are non-natural or genetically engineered polypeptides having an activity comprising, for example, a vinylisomerase-dehydratase, an alkenol dehydratase, a linalool dehydratase and/or a crotyl alcohol dehydratase activity, or a combination thereof. In alternative embodiments, also provided are non-natural or genetically engineered nucleic acids (polynucleotides) encoding polypeptides described herein, expression or cloning vehicles comprising or having contained therein nucleic acids as described herein, and non-natural or genetically engineered cells comprising or having contained therein nucleic acids as described herein. In alternative embodiments, also provided are methods for making various organic compounds, including methyl vinyl carbinol and butadiene.

公开(公告)号：US20210277426A1

公开(公告)日：2021-09-09

申请号：US16953050

申请日：2020-11-19

Applicant: Genomatica, Inc.

Inventor： Stephanie J. Culler ,  Nicholas Eakley ,  Kevin Gregory Hoff ,  Robin E. Osterhout ,  Priti Pharkya ,  Erik C. Ralph ,  Stephen J. Van Dien

IPC: C12P5/02 ,  C12N9/04 ,  C12N9/02 ,  C12P7/16 ,  C12P7/04

Abstract: Disclosed are methods and engineered microorganisms that enhance or improve the production of crotyl alcohol. The engineered microorganisms include genetic modifications in alcohol dehydrogenase, alkene reductase or both enzymatic activities. By such genetic modifications, a crotyl alcohol production pathway is provided or improved.

公开(公告)号：US20210002673A1

公开(公告)日：2021-01-07

申请号：US16888339

申请日：2020-05-29

Applicant: Genomatica, Inc.

Inventor： Stephanie J. Culler ,  Mark J. Burk ,  Robin E. Osterhout ,  Priti Pharkya

IPC: C12P7/04 ,  C12N15/52 ,  C12P5/02 ,  C12P7/40 ,  C12N9/90 ,  C12N9/88 ,  C12N9/04

Abstract: The invention provides a non-naturally occurring microbial organism having a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol, pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a pathway. The invention additionally provides a method for producing butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol. The method can include culturing a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol-producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a pathway enzyme in a sufficient amount, and under conditions and for a sufficient period of time to produce butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol.

公开(公告)号：US10669561B2

公开(公告)日：2020-06-02

申请号：US15323360

申请日：2015-07-02

Applicant: Genomatica, Inc.

Inventor： Stephanie J. Culler ,  Mark J. Burk ,  Robin E. Osterhout ,  Priti Pharkya

IPC: C12N15/00 ,  C12N1/20 ,  C12N9/00 ,  C12N9/02 ,  C12N9/88 ,  C12N9/90 ,  C07H21/04 ,  C12P7/04 ,  C12N15/52 ,  C12P5/02 ,  C12P7/40 ,  C12N9/04

Abstract: The invention provides a non-naturally occurring microbial organism having a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol, pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a pathway. The invention additionally provides a method for producing butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol. The method can include culturing a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol-producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a pathway enzyme in a sufficient amount, and under conditions and for a sufficient period of time to produce butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol.

公开(公告)号：US20180135082A1

公开(公告)日：2018-05-17

申请号：US15564327

申请日：2016-04-07

Applicant: Genomatica, Inc.

Inventor： Stephanie J. Culler ,  Nicholas Eakley ,  Kevin Gregory Hoff ,  Robin E. Osterhout ,  Priti Pharkya ,  Erik C. Ralph ,  Stephen Van Dien

IPC: C12P5/02 ,  C12P7/16 ,  C12N9/04 ,  C12N9/02

CPC classification number: C12P5/026 ,  C12N9/0006 ,  C12N9/001 ,  C12P7/04 ,  C12P7/16 ,  Y02E50/10

Abstract: Disclosed are methods and engineered microorganisms that enhance or improve the production of crotyl alcohol. The engineered microorganisms include genetic modifications in alcohol dehydrogenase, alkene reductase or both enzymatic activities. By such genetic modifications, a crotyl alcohol production pathway is provided or improved.

公开(公告)号：US20170166931A1

公开(公告)日：2017-06-15

申请号：US15323360

申请日：2015-07-02

Applicant: Genomatica, Inc.

Inventor： Stephanie J. Culler ,  Mark J. Burk ,  Robin E. Osterhout ,  Priti Pharkya

IPC: C12P7/04 ,  C12P5/02 ,  C12N15/52

Abstract: The invention provides a non-naturally occurring microbial organism having a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol, pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a pathway. The invention additionally provides a method for producing butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol. The method can include culturing a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol-producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a pathway enzyme in a sufficient amount, and under conditions and for a sufficient period of time to produce butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol.