公开(公告)号：US10941454B2

公开(公告)日：2021-03-09

申请号：US15578488

申请日：2016-05-26

Applicant: GENOMATICA, INC. ,  BRASKEM S.A.

Inventor： Stephanie J. Culler ,  Robert J. Haselbeck ,  Harish Nagarajan ,  Iuri Estrada Gouvea ,  Daniel Johannes Koch ,  Mateus Schreiner Garcez Lopes ,  Lucas Pedersen Parizzi

IPC: C12N9/88 ,  C12N9/90 ,  C07K16/40 ,  C08F36/06 ,  C12N15/82 ,  C12P5/02 ,  C12P7/16

Abstract: In alternative embodiments, provided are non-natural or genetically engineered vinylisomerase-dehydratase enzymes, including alkenol dehydratases, linalool dehydratases and crotyl alcohol dehydratases. In alternative embodiments, provided are non-natural or genetically engineered polypeptides having an activity comprising, for example, a vinylisomerase-dehydratase, an alkenol dehydratase, a linalool dehydratase and/or a crotyl alcohol dehydratase activity, or a combination thereof. In alternative embodiments, also provided are non-natural or genetically engineered nucleic acids (polynucleotides) encoding polypeptides described herein, expression or cloning vehicles comprising or having contained therein nucleic acids as described herein, and non-natural or genetically engineered cells comprising or having contained therein nucleic acids as described herein. In alternative embodiments, also provided are methods for making various organic compounds, including methyl vinyl carbinol and butadiene.

公开(公告)号：US20150064760A1

公开(公告)日：2015-03-05

申请号：US14479071

申请日：2014-09-05

Applicant: BRASKEM S.A.

Inventor： Johana Rincones Perez ,  Juan Diego Rojas Rojas ,  Ane Fernanda Beraldi Zeidler ,  Aline Silva Romao Dumaresq ,  Marilene Elizabete Pavan Rodrigues ,  Iuri Estrada Gouvea ,  Felipe Galzerani ,  Daniel Johannes Koch ,  Lucas Pedersen Parizzi ,  Mateus Schreiner Garcez Lopes ,  Thomas Martin Halder ,  Antonio Luis Ribeiro De Castro Morschbacker ,  Avram Michael Slovic

IPC: C12P5/02 ,  C12P7/04 ,  C12P7/18 ,  C12N15/63

CPC classification number: C12P7/18 ,  C07K14/395 ,  C12N15/63 ,  C12P5/026 ,  C12P7/04

Abstract: The present disclosure provides a non-naturally occurring microorganism comprising: one or more polynucleotides encoding one or more enzymes in a pathway that produces acetyl-CoA; one or more polynucleotides encoding one or more enzymes in a pathway that catalyze a conversion of crotonyl alcohol, 5-hydroxy-3-ketovaleryl-CoA, 3-ketopent-4-enoyl-CoA, or 3,5-ketovaleryl-CoA to butadiene; one or more polynucleotides encoding one or more enzymes in a pathway that catalyze a conversion of dihydroxyacetone-phosphate to 1-propanol and/or 1,2-propanediol, wherein the microorganism has reduced levels of pyruvate decarboxylase enzymatic activity (e.g., the microorganism comprises a disruption of one or more enzymes that decarboxylate pyruvate and/or a disruption of one or more transcription factors of one or more enzymes that decarboxylate pyruvate), and wherein the microorganism is capable of growing on a C6 sugar as a sole carbon source under anaerobic conditions. Also provided are methods of using the disclosed non-naturally occurring microorganisms in methods for the coproduction of butadiene and 1-propanol and/or 1,2-propanediol.

Abstract translation: 本公开提供了非天然存在的微生物，其包含：在产生乙酰辅酶A的途径中编码一种或多种酶的一种或多种多核苷酸; 在催化巴豆醇，5-羟基-3-酮戊酰基-CoA，3-酮戊-4-烯酰基-CoA或3,5-酮戊酰基-CoA转化为丁二烯的途径中编码一种或多种酶的一种或多种多核苷酸 ; 一种或多种编码一种或多种酶的多核苷酸，其在催化二羟丙基磷酸盐转化为1-丙醇和/或1,2-丙二醇的途径中，其中微生物具有降低的丙酮酸脱羧酶水平的酶活性（例如，微生物包含 破坏一种或多种脱羧酸丙酮酸酶和/或破坏一种或多种丙酮酸脱羧酸酶的一种或多种转录因子的酶），并且其中所述微生物能够生长在C6糖作为唯一碳源的厌氧 条件。 还提供了在共同生产丁二烯和1-丙醇和/或1,2-丙二醇的方法中使用所公开的非天然存在的微生物的方法。

公开(公告)号：US12163008B2

公开(公告)日：2024-12-10

申请号：US17333317

申请日：2021-05-28

Applicant: Braskem S.A.

Inventor： Felipe Cicaroni Fernandes ,  Iuri Estrada Gouvea ,  Antonio Rodolfo, Jr. ,  Mateus Schreiner Garcez Lopes

IPC: C07D307/68 ,  C08J3/12 ,  C08J3/18 ,  C08K5/00 ,  C08K5/1535 ,  C08L27/06

Abstract: The present disclosure generally relates to a compound of dialkyl ester of 2,4-furandicarboxylic acid, a method of preparing the compound, a polymer composition comprising a polymer and the compound, a method of preparing the polymer composition, a polymer product comprising the polymer composition and a method of using the compound as a plasticizer in the polymer product. The dialkyl ester of 2,4-furandicarboxylic acid of the present disclosure has greater plasticizing efficiency in a polymer composition that that of the standard phthalate and terephthalate-based plasticizers. The polymer product plasticized with the dialkyl ester of 2,4-furandicarboxylic acid may have improved flexibility, durability, processability and safety as compared to the same polymer product plasticized with conventional phthalate and terephthalate-based plasticizers.

公开(公告)号：US12037609B2

公开(公告)日：2024-07-16

申请号：US18084888

申请日：2022-12-20

Applicant: Braskem S.A.

Inventor： Verônica Leite Queiroz ,  Lucas Pedersen Parizzi ,  Iuri Estrada Gouvea ,  Debora Noma Okamoto ,  Rafael Victório Carvalho Guido ,  Alessandro Silva Nascimento ,  Igor Polikarpov

IPC: C12N9/04 ,  C12N1/21 ,  C12N15/52

CPC classification number: C12N9/0006 ,  C12N15/52 ,  C12Y101/01001

Abstract: The present disclosure relates to biological processes and systems for the production of isopropanol and/or acetone utilizing modified alcohol dehydrogenases that exhibit increased activity with NADH as a cofactor. The disclosure further relates to polynucleotides and polypeptides of the modified alcohol dehydrogenases, and host cells containing the polynucleotides and expressing the polypeptides.

公开(公告)号：US12018119B2

公开(公告)日：2024-06-25

申请号：US17586535

申请日：2022-01-27

Applicant: Braskem S.A.

Inventor： Felipe Cicaroni Fernandes ,  Iuri Estrada Gouvea ,  Bárbara Mano

IPC: C08G63/40 ,  C08G63/183

CPC classification number: C08G63/183 ,  C08G63/40

Abstract: The present disclosure provides a copolyester including a dicarboxylic acid component A comprising a terephthalic acid residue or ester-forming derivative thereof, or a mixture thereof, and a 2,4-furandicarboxylic acid residue or ester-forming derivative thereof, or a mixture thereof, and a diol component B comprising an alkanediol residue having from 2 to 22 carbon atoms, wherein the dicarboxylic acid component A has a total molar content, and wherein the 2,4-furandicarboxylic acid residue or ester-forming derivative thereof, is present in an amount of from 0.1 to 10 mol %, with respect to the total molar content of the dicarboxylic acid component A. Inventive copolyesters have a slower crystallization rate, a higher gas barrier to CO2 and O2 and a higher ratio of 14C to 12C as measured by ASTM D6866 when compared to a comparable copolyester comprising isophthalic acid instead of 2,4-furandicarboxylic acid.

公开(公告)号：US12006523B2

公开(公告)日：2024-06-11

申请号：US17545616

申请日：2021-12-08

Applicant: Braskem S.A.

Inventor： Iuri Estrada Gouvea ,  Paulo Moises Raduan Alexandrino

IPC: C12P17/04 ,  C07D307/68 ,  C12N1/21 ,  C12N9/02 ,  C12N9/04 ,  C12N9/08 ,  C12N9/16 ,  C12N9/88

CPC classification number: C12P17/04 ,  C07D307/68 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0065 ,  C12N9/16 ,  C12N9/88 ,  C12Y101/01 ,  C12Y102/01 ,  C12Y101/01001 ,  C12Y101/03 ,  C12Y102/01003 ,  C12Y102/01004 ,  C12Y102/01005 ,  C12Y207/01 ,  C12Y301/03 ,  C12Y402/03

Abstract: The present disclosure provides recombinant microorganisms and methods for the production of 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA from a carbon source. The method provides for engineered microorganisms that express endogenous and/or exogenous nucleic acid molecules that catalyze the conversion of a carbon source into 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA. The disclosure further provides methods of producing polymers derived from 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA.

公开(公告)号：US10000744B2

公开(公告)日：2018-06-19

申请号：US15023579

申请日：2014-09-23

Applicant: BRASKEM S.A. ,  Avram Michael Slovic

Inventor： Avram Michael Slovic ,  Iuri Estrada Gouvea ,  Daniel Johannes Koch ,  Felipe Galzerani

IPC: C12N1/19 ,  C12N1/21 ,  C12N9/16 ,  C12N9/10 ,  C12P7/04

CPC classification number: C12N9/16 ,  C12N9/13 ,  C12P7/04 ,  C12Y301/02011

Abstract: The disclosure provides engineered enzymes that are capable of mediating the conversion of acetoacetyl-CoA to acetoacetate that do not react with the same order of magnitude with acetyl-CoA as they do with acetoacetyl-CoA (e.g., the engineered enzymes have a specific acetoacetyl-CoA hydrolase activity at least 10× higher than its acetyl-CoA hydrolase activity). Additionally, the disclosure provides modified microorganisms that comprise the engineered enzymes disclosed herein and methods of using same.

公开(公告)号：US11566270B2

公开(公告)日：2023-01-31

申请号：US17219792

申请日：2021-03-31

Applicant: Braskem S.A.

Inventor： Paulo Moises Raduan Alexandrino ,  Iuri Estrada Gouvea ,  Veronica Leite Queiroz ,  Felipe Cicaroni Fernandes ,  Bárbara Mano

IPC: C12P17/04 ,  C07D307/68 ,  C12N9/88 ,  C12N9/04 ,  C12N9/02 ,  C12N9/08 ,  C12N9/16

Abstract: The present disclosure provides recombinant microorganisms and methods for the production of 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA from a carbon source. The method provides for engineered microorganisms that express endogenous and/or exogenous nucleic acid molecules that catalyze the conversion of a carbon source into 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA. The disclosure further provides methods of producing polymers derived from 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA.

公开(公告)号：US20180346936A1

公开(公告)日：2018-12-06

申请号：US16042594

申请日：2018-07-23

Applicant: Braskem S.A.

Inventor： Mateus Schreiner Garcez Lopes ,  Avram Michael Slovic ,  Iuri Estrada Gouvea ,  Johana Rincones Perez ,  Lucas Pedersen Parizzi

IPC: C12P5/02 ,  C12N9/88 ,  C12N15/52

CPC classification number: C12P5/026 ,  C12N9/88 ,  C12N15/52 ,  C12P5/02 ,  C12Y402/01127

Abstract: The present disclosure generally relates to methods of using microorganisms that comprise one or more polynucleotides coding for enzymes in one or more pathways that catalyze a conversion of a fermentable carbon source to butadiene and products and processes derived therefrom.

公开(公告)号：US20180327727A1

公开(公告)日：2018-11-15

申请号：US16011041

申请日：2018-06-18

Applicant: Braskem S.A.

Inventor： Avram Michael Slovic ,  Iuri Estrada Gouvea ,  Daniel Johannes Koch ,  Felipe Galzerani

IPC: C12N9/16 ,  C12P7/04 ,  C12N9/10

Abstract: The disclosure provides engineered enzymes that are capable of mediating the conversion of acetoacetyl-CoA to acetoacetate that do not react with the same order of magnitude with acetyl-CoA as they do with acetoacetyl-CoA (e.g., the engineered enzymes have a specific acetoacetyl-CoA hydrolase activity at least 10x higher than its acetyl-CoA hydrolase activity). Additionally, the disclosure provides modified microorganisms that comprise the engineered enzymes disclosed herein and methods of using same.