公开(公告)号：US20160024139A1

公开(公告)日：2016-01-28

申请号：US14775760

申请日：2014-03-13

Applicant: GLAXOSMITHKLINE BIOLOGICALS SA

Inventor： Francesco BERLANDA SCORZA ,  Yingxia WEN ,  Andrew GEALL ,  Frederick PORTER

IPC: C07H21/02 ,  B01D15/36 ,  B01D15/38 ,  C07H1/06

CPC classification number: C07H21/02 ,  B01D15/36 ,  B01D15/38 ,  B01D15/3847 ,  B01D61/14 ,  B01D2015/3838 ,  C07H1/06 ,  C12N15/101 ,  C12N15/1017

Abstract: Methods for purifying RNA from a sample, comprising one or more steps of tangential flow filtration, hydroxyapatite chromatography, core bead flow-through chromatography, or any combinations thereof. These techniques are useful individually, but show very high efficiency when used in combination, or when performed in particular orders. The methods can purify RNA in a highly efficient manner without unduly compromising potency or stability, to provide compositions in which RNA is substantially cleared of contaminants. Moreover, they can be performed without the need for organic solvents.

Abstract translation: 用于从样品中纯化RNA的方法，包括一个或多个切向流过滤步骤，羟基磷灰石层析，芯珠流通层析或其任何组合。 这些技术可以单独使用，但是当组合使用时，或者特别是以特定顺序执行时，显示出非常高的效率。 该方法可以高效地纯化RNA，而不会不利地影响效力或稳定性，以提供RNA基本上不含污染物的组合物。 此外，它们可以在不需要有机溶剂的情况下进行。

公开(公告)号：US20210214388A1

公开(公告)日：2021-07-15

申请号：US17157150

申请日：2021-01-25

Applicant: GLAXOSMITHKLINE BIOLOGICALS SA

Inventor： Francesco BERLANDA SCORZA ,  Yingxia WEN ,  Andrew GEALL ,  Frederick PORTER

IPC: C07H21/02 ,  B01D15/36 ,  B01D15/38 ,  C07H1/06 ,  C12N15/10 ,  B01D61/14

Abstract: Methods for purifying RNA from a sample, comprising one or more steps of tangential flow filtration, hydroxyapatite chromatography, core bead flow-through chromatography, or any combinations thereof. These techniques are useful individually, but show very high efficiency when used in combination, or when performed in particular orders. The methods can purify RNA in a highly efficient manner without unduly compromising potency or stability, to provide compositions in which RNA is substantially cleared of contaminants. Moreover, they can be performed without the need for organic solvents.

公开(公告)号：US20240300994A1

公开(公告)日：2024-09-12

申请号：US18664453

申请日：2024-05-15

Applicant: GLAXOSMITHKLINE BIOLOGICALS SA

Inventor： Francesco BERLANDA SCORZA ,  Yingxia WEN ,  Andrew GEALL ,  Frederick PORTER

IPC: C07H21/02 ,  B01D15/36 ,  B01D15/38 ,  B01D61/14 ,  C07H1/06 ,  C07H21/00 ,  C07H21/04 ,  C12N15/10

CPC classification number: C07H21/02 ,  B01D15/36 ,  B01D15/38 ,  B01D15/3847 ,  B01D61/14 ,  C07H1/06 ,  C07H21/00 ,  C07H21/04 ,  C12N15/101 ,  C12N15/1017 ,  B01D2015/3838

Abstract: Methods for purifying RNA from a sample, comprising one or more steps of tangential flow filtration, hydroxyapatite chromatography, core bead flow-through chromatography, or any combinations thereof. These techniques are useful individually, but show very high efficiency when used in combination, or when performed in particular orders. The methods can purify RNA in a highly efficient manner without unduly compromising potency or stability, to provide compositions in which RNA is substantially cleared of contaminants. Moreover, they can be performed without the need for organic solvents.

公开(公告)号：US20180360949A1

公开(公告)日：2018-12-20

申请号：US16061729

申请日：2016-12-16

Applicant: GLAXOSMITHKLINE BIOLOGICALS, SA

Inventor： Susan BARNETT ,  Marguerite Christine KOUTSOUKOS ,  Clarisse Marie-Madeleine LORIN ,  Frederick PORTER ,  Zihao WANG ,  Ying ZHANG

IPC: A61K39/21 ,  A61K39/295 ,  C07K14/16

Abstract: The invention provides, inter alia, compositions useful for, e.g., raising an immune response to HIV-1, and associated methods of raising an immune response to HIV in a mammalian subject. In some embodiments, the compositions are bivalent immunogenic compositions comprising two (or, in some embodiments more than two) human immunodeficiency virus (HIV) clade C envelope gp120 polypeptide antigens, together with a liposome-based adjuvant, such as the adjuvant known as AS01.