公开(公告)号：US20160348162A1

公开(公告)日：2016-12-01

申请号：US15058702

申请日：2016-03-02

Applicant: GEN-PROBE INCORPORATED

Inventor： Steven T. BRENTANO ,  Dmitry LYAKHOV ,  Norman C. NELSON ,  James D. CARLSON ,  Michael M. BECKER ,  Lyle J. ARNOLD, Jr.

IPC: C12Q1/68

CPC classification number: C12Q1/6865 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q2549/119 ,  C12Q2537/143 ,  C12Q2531/143

Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.

公开(公告)号：US20170342491A1

公开(公告)日：2017-11-30

申请号：US15595353

申请日：2017-05-15

Applicant: GEN-PROBE INCORPORATED

Inventor： Steven T. BRENTANO ,  Dmitry LYAKHOV ,  James D. CARLSON ,  Norman C. NELSON ,  Lyle J. ARNOLD ,  Michael M. BECKER

IPC: C12Q1/68

CPC classification number: C12Q1/6881 ,  C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6865 ,  C12Q2525/155 ,  C12Q2525/186 ,  C12Q2525/197 ,  C12Q2531/143 ,  C12Q2565/543

Abstract: Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.

公开(公告)号：US20130309673A1

公开(公告)日：2013-11-21

申请号：US13956158

申请日：2013-07-31

Applicant: Gen-Probe Incorporated

Inventor： Steven T. BRENTANO ,  Dmitry LYAKHOV ,  Norman C. NELSON ,  James D. CARLSON ,  Michael M. BECKER ,  Lyle J. ARNOLD, JR.

IPC: C12Q1/68

CPC classification number: C12Q1/6865 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q2549/119 ,  C12Q2537/143 ,  C12Q2531/143

Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.

Abstract translation: 用于进行扩增反应的组合物，反应混合物和方法，包括多重扩增反应，其中所述方法包括使用包含连接的第一和第二扩增寡聚体成员的一种或多种扩增寡聚体复合物。 一方面，将扩增寡聚物复合物与靶核酸杂交，然后捕获具有杂交扩增寡聚物复合物的靶核酸，并洗涤其它组分。 靶核酸的靶序列被预扩增以产生第一扩增产物。 第一扩增产物在一个或多个二级扩增反应中扩增以产生第二扩增产物。

公开(公告)号：US20180208981A1

公开(公告)日：2018-07-26

申请号：US15902819

申请日：2018-02-22

Applicant: GEN-PROBE INCORPORATED

Inventor： Steven T. BRENTANO ,  Dmitry LYAKHOV ,  Norman C. NELSON ,  James D. CARLSON ,  Michael M. BECKER ,  Lyle J. ARNOLD, JR.

IPC: C12Q1/6865 ,  C12Q1/6853 ,  C12Q1/6844 ,  C12Q1/6848

CPC classification number: C12Q1/6865 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q2549/119 ,  C12Q2537/143 ,  C12Q2531/143

Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.

公开(公告)号：US20170321273A1

公开(公告)日：2017-11-09

申请号：US15417736

申请日：2017-01-27

Applicant: GEN-PROBE INCORPORATED

Inventor： Steven T. BRENTANO ,  Dmitry LYAKHOV ,  James D. CARLSON ,  Norman C. NELSON ,  Lyle J. ARNOLD ,  Michael M. BECKER

IPC: C12Q1/68

CPC classification number: C12Q1/6881 ,  C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6865 ,  C12Q2525/155 ,  C12Q2525/186 ,  C12Q2525/197 ,  C12Q2531/143 ,  C12Q2565/543

Abstract: Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.

公开(公告)号：US20140127700A1

公开(公告)日：2014-05-08

申请号：US14109709

申请日：2013-12-17

Applicant: GEN-PROBE INCORPORATED

Inventor： Steven T. BRENTANO ,  Dmitry LYAKHOV ,  James D. CARLSON ,  Norman C. NELSON ,  Lyle J. ARNOLD, JR. ,  Michael M. BECKER

IPC: C12Q1/68

CPC classification number: C12Q1/6881 ,  C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6865 ,  C12Q2525/155 ,  C12Q2525/186 ,  C12Q2525/197 ,  C12Q2531/143 ,  C12Q2565/543

Abstract: Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.

Abstract translation: 公开了用于体外核酸扩增的组合物，其包括目标特异性通用（TSU）启动子引物或启动子提供者寡核苷酸，其包括与扩增的靶序列特异性杂交的靶特异性（TS）序列和通用 （U）序列，通过使用通用序列的引物引入扩增的序列。 公开了体外核酸扩增方法，其中使用一种或多种TSU寡核苷酸将U序列连接到目标捕获步骤中的靶核酸，然后在基本上等温条件下进行的随后扩增步骤中使用U序列引物 制备含有指示样品中靶核酸存在的U序列的扩增产物。