公开(公告)号：US20160348162A1

公开(公告)日：2016-12-01

申请号：US15058702

申请日：2016-03-02

Applicant: GEN-PROBE INCORPORATED

Inventor： Steven T. BRENTANO ,  Dmitry LYAKHOV ,  Norman C. NELSON ,  James D. CARLSON ,  Michael M. BECKER ,  Lyle J. ARNOLD, Jr.

IPC: C12Q1/68

CPC classification number: C12Q1/6865 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q2549/119 ,  C12Q2537/143 ,  C12Q2531/143

Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.

公开(公告)号：US20170342491A1

公开(公告)日：2017-11-30

申请号：US15595353

申请日：2017-05-15

Applicant: GEN-PROBE INCORPORATED

Inventor： Steven T. BRENTANO ,  Dmitry LYAKHOV ,  James D. CARLSON ,  Norman C. NELSON ,  Lyle J. ARNOLD ,  Michael M. BECKER

IPC: C12Q1/68

CPC classification number: C12Q1/6881 ,  C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6865 ,  C12Q2525/155 ,  C12Q2525/186 ,  C12Q2525/197 ,  C12Q2531/143 ,  C12Q2565/543

Abstract: Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.

公开(公告)号：US20130309673A1

公开(公告)日：2013-11-21

申请号：US13956158

申请日：2013-07-31

Applicant: Gen-Probe Incorporated

Inventor： Steven T. BRENTANO ,  Dmitry LYAKHOV ,  Norman C. NELSON ,  James D. CARLSON ,  Michael M. BECKER ,  Lyle J. ARNOLD, JR.

IPC: C12Q1/68

CPC classification number: C12Q1/6865 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q2549/119 ,  C12Q2537/143 ,  C12Q2531/143

Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.

Abstract translation: 用于进行扩增反应的组合物，反应混合物和方法，包括多重扩增反应，其中所述方法包括使用包含连接的第一和第二扩增寡聚体成员的一种或多种扩增寡聚体复合物。 一方面，将扩增寡聚物复合物与靶核酸杂交，然后捕获具有杂交扩增寡聚物复合物的靶核酸，并洗涤其它组分。 靶核酸的靶序列被预扩增以产生第一扩增产物。 第一扩增产物在一个或多个二级扩增反应中扩增以产生第二扩增产物。

公开(公告)号：US20170009284A1

公开(公告)日：2017-01-12

申请号：US15277167

申请日：2016-09-27

Applicant: GEN-PROBE INCORPORATED

Inventor： James D. CARLSON ,  Steven T. BRENTANO

IPC: C12Q1/68 ,  C12Q1/70

CPC classification number: C12Q1/6865 ,  C12Q1/706 ,  C12Q2600/158 ,  C12Q2600/16 ,  Y02A50/451 ,  Y02A50/54

Abstract: Nucleic acid oligomeric sequences and in vitro nucleic acid amplification and detection methods for detecting the presence of HAV RNA sequences in samples are disclosed. Kits comprising nucleic acid oligomers for amplifying and detecting HAV nucleic acid sequences are disclosed.

Abstract translation: 公开了用于检测样品中HAV RNA序列的存在的核酸寡聚序列和体外核酸扩增和检测方法。 公开了包含用于扩增和检测HAV核酸序列的核酸低聚物的试剂盒。

公开(公告)号：US20150051092A1

公开(公告)日：2015-02-19

申请号：US14031353

申请日：2013-09-19

Applicant: Gen-Probe Incorporated

Inventor： James D. CARLSON ,  Steven T. BRENTANO

IPC: C12Q1/70

CPC classification number: C12Q1/6865 ,  C12Q1/706 ,  C12Q2600/158 ,  C12Q2600/16 ,  Y02A50/451 ,  Y02A50/54

Abstract: Nucleic acid oligomeric sequences and in vitro nucleic acid amplification and detection methods for detecting the presence of HAV RNA sequences in samples are disclosed. Kits comprising nucleic acid oligomers for amplifying and detecting HAV nucleic acid sequences are disclosed.

公开(公告)号：US20150037785A1

公开(公告)日：2015-02-05

申请号：US13924087

申请日：2013-06-21

Applicant: Gen-Probe Incorporated

Inventor： Patricia GORDON ,  Nick M. CARTER ,  Steven T. BRENTANO ,  Philip W. HAMMOND

IPC: C12Q1/70

CPC classification number: C12Q1/708 ,  C12Q1/6865 ,  C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/16

Abstract: Hybridization assay detection probes targeted to HPV Type 16 nucleic acid sequences which are particularly useful to aid in detecting HPV type 16 are described. The oligonucleotides can aid in detecting HPV Type 16 by acting singly or as part of a detection probe mixture. Compositions, reaction mixtures and methods of use are provided.

Abstract translation: 描述了特别可用于帮助检测HPV 16型的HPV 16型核酸序列的杂交测定检测探针。 寡核苷酸可以通过单独作用或作为检测探针混合物的一部分来帮助检测HPV 16型。 提供了组合物，反应混合物和使用方法。

公开(公告)号：US20130095470A1

公开(公告)日：2013-04-18

申请号：US13712789

申请日：2012-12-12

Applicant: GEN-PROBE INCORPORATED

Inventor： Steven T. BRENTANO ,  Margarita BATRANINA-KAMINSKY ,  Cynthia S. HASSELKUS-LIGHT ,  Daniel P. KOLK

IPC: C12Q1/70

CPC classification number: C12Q1/701 ,  C12Q1/6865 ,  C12Q2525/161 ,  C12Q2525/113

Abstract: Nucleic acid oligomers specific for human parvovirus B19 genomic DNA are disclosed. An assay for amplifying and detecting human parvovirus B19 nucleic acid in biological specimens is disclosed. Compositions for detecting the presence of parvovirus B19 genomic DNA in human biological specimens are disclosed.

Abstract translation: 公开了对人细小病毒B19基因组DNA特异性的核酸低聚物。 公开了用于在生物样品中扩增和检测人细小病毒B19核酸的测定法。 公开了用于检测人生物标本中细小病毒B19基因组DNA存在的组合物。

公开(公告)号：US20180208981A1

公开(公告)日：2018-07-26

申请号：US15902819

申请日：2018-02-22

Applicant: GEN-PROBE INCORPORATED

Inventor： Steven T. BRENTANO ,  Dmitry LYAKHOV ,  Norman C. NELSON ,  James D. CARLSON ,  Michael M. BECKER ,  Lyle J. ARNOLD, JR.

IPC: C12Q1/6865 ,  C12Q1/6853 ,  C12Q1/6844 ,  C12Q1/6848

CPC classification number: C12Q1/6865 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q2549/119 ,  C12Q2537/143 ,  C12Q2531/143

Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.

公开(公告)号：US20170321273A1

公开(公告)日：2017-11-09

申请号：US15417736

申请日：2017-01-27

Applicant: GEN-PROBE INCORPORATED

Inventor： Steven T. BRENTANO ,  Dmitry LYAKHOV ,  James D. CARLSON ,  Norman C. NELSON ,  Lyle J. ARNOLD ,  Michael M. BECKER

IPC: C12Q1/68

CPC classification number: C12Q1/6881 ,  C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6865 ,  C12Q2525/155 ,  C12Q2525/186 ,  C12Q2525/197 ,  C12Q2531/143 ,  C12Q2565/543

Abstract: Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.

公开(公告)号：US20140127700A1

公开(公告)日：2014-05-08

申请号：US14109709

申请日：2013-12-17

Applicant: GEN-PROBE INCORPORATED

Inventor： Steven T. BRENTANO ,  Dmitry LYAKHOV ,  James D. CARLSON ,  Norman C. NELSON ,  Lyle J. ARNOLD, JR. ,  Michael M. BECKER

IPC: C12Q1/68

CPC classification number: C12Q1/6881 ,  C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6865 ,  C12Q2525/155 ,  C12Q2525/186 ,  C12Q2525/197 ,  C12Q2531/143 ,  C12Q2565/543

Abstract: Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.

Abstract translation: 公开了用于体外核酸扩增的组合物，其包括目标特异性通用（TSU）启动子引物或启动子提供者寡核苷酸，其包括与扩增的靶序列特异性杂交的靶特异性（TS）序列和通用 （U）序列，通过使用通用序列的引物引入扩增的序列。 公开了体外核酸扩增方法，其中使用一种或多种TSU寡核苷酸将U序列连接到目标捕获步骤中的靶核酸，然后在基本上等温条件下进行的随后扩增步骤中使用U序列引物 制备含有指示样品中靶核酸存在的U序列的扩增产物。