公开(公告)号：US20220151221A1

公开(公告)日：2022-05-19

申请号：US17497330

申请日：2021-10-08

Applicant: Genentech, Inc.

Inventor： Angela Meier ,  Steven J. Meier ,  Phillip Duffy ,  Marcia Coyne ,  Kara Calhoun

IPC: A01N1/02 ,  C12N5/00 ,  C12N5/071

Abstract: Provided herein are methods of freezing mammalian cells for storage or improving thaw recovery of cell banks comprising freezing mammalian cells in a freezing medium having a pH of 6.7 to 8.5.

公开(公告)号：US11912823B2

公开(公告)日：2024-02-27

申请号：US17319886

申请日：2021-05-13

Applicant: Genentech, Inc.

Inventor： Ganesh Prasadh Vissvesvaran ,  Robert David Kiss ,  Steven J. Meier ,  Inchan Kwon ,  Kara Calhoun ,  Kate Winchester ,  Amelia Adams ,  Marion Glenn ,  Stefan Koenig ,  Alan Deese

IPC: C08G65/30 ,  C12N5/00 ,  C07K16/00

CPC classification number: C08G65/30 ,  C07K16/00 ,  C12N5/0018 ,  C07K2317/14 ,  C08G2650/50 ,  C12N2500/50

Abstract: Provided herein are methods of preparing a poloxamer for use in a cell culture medium. Also provided herein are cell culture media containing the poloxamer prepared by the methods herein, as well as methods of using the media for cell culturing and polypeptide production from cells.

公开(公告)号：US10602739B2

公开(公告)日：2020-03-31

申请号：US15399666

申请日：2017-01-05

Applicant: Genentech, Inc.

Inventor： Angela Meier ,  Steven J. Meier ,  Phillip Duffy ,  Marcia Coyne ,  Kara Calhoun

IPC: A01N1/02 ,  C12N5/00 ,  C12N5/071

Abstract: Provided herein are methods of freezing mammalian cells for storage or improving thaw recovery of cell banks comprising freezing mammalian cells in a freezing medium having a pH of 6.7 to 8.5.

公开(公告)号：US12016328B2

公开(公告)日：2024-06-25

申请号：US17497330

申请日：2021-10-08

Applicant: Genentech, Inc.

Inventor： Angela Meier ,  Steven J. Meier ,  Phillip Duffy ,  Marcia Coyne ,  Kara Calhoun

IPC: A01N1/02 ,  C12N5/00 ,  C12N5/071 ,  C12N5/10 ,  C12N15/16

CPC classification number: A01N1/0221 ,  A01N1/0284 ,  C12N5/0018 ,  C12N5/0682 ,  C12N2510/02

Abstract: Provided herein are methods of freezing mammalian cells for storage or improving thaw recovery of cell banks comprising freezing mammalian cells in a freezing medium having a pH of 6.7 to 8.5.

公开(公告)号：US11034793B2

公开(公告)日：2021-06-15

申请号：US15273155

申请日：2016-09-22

Applicant: Genentech, Inc.

Inventor： Ganesh Prasadh Vissvesvaran ,  Robert David Kiss ,  Steven J. Meier ,  Inchan Kwon ,  Kara Calhoun ,  Kate Winchester ,  Amelia Adams ,  Marion Glenn ,  Stefan Koenig ,  Alan Deese

IPC: C08G65/30 ,  C12N5/00 ,  C07K16/00

Abstract: Provided herein are methods of preparing a poloxamer for use in a cell culture medium. Also provided herein are cell culture media containing the poloxamer prepared by the methods herein, as well as methods of using the media for cell culturing and polypeptide production from cells.