公开(公告)号：US10184106B2

公开(公告)日：2019-01-22

申请号：US15290986

申请日：2016-10-11

Applicant: Genentech, Inc.

Inventor： Masaru Ken Shiratori ,  Robert David Kiss ,  Hardayal Prashad ,  Raquel Iverson ,  Justin Bourret ,  Michael Kim ,  Salim Charaniya

IPC: C12N7/00 ,  C12N1/36

Abstract: The invention provides for methods of viral inactivation using high temperature short time (HTST) treatment and adjustment of various parameters such that generation of precipitate and depositions of precipitate are reduced and/or minimized.

公开(公告)号：US09493744B2

公开(公告)日：2016-11-15

申请号：US13844051

申请日：2013-03-15

Applicant: Genentech, Inc.

Inventor： Masaru Ken Shiratori ,  Robert David Kiss ,  Hardayal Prashad ,  Raquel Iverson ,  Justin Bourret ,  Michael Kim ,  Salim Charaniya

IPC: C12Q1/70 ,  C12N7/00 ,  C12N1/36

CPC classification number: C12N1/36 ,  C12N7/00 ,  C12N2750/14061 ,  C12N2760/12061 ,  C12N2760/16061 ,  C12N2760/18061 ,  C12N2760/20061 ,  C12N2770/16061 ,  C12N2770/32061 ,  C12N2770/36061

Abstract: The invention provides for methods of viral inactivation using high temperature short time (HTST) treatment and adjustment of various parameters such that generation of precipitate and depositions of precipitate are reduced and/or minimized.

Abstract translation: 本发明提供使用高温短时间（HTST）处理和调整各种参数的病毒灭活方法，使得沉淀物的产生和沉淀的沉积减少和/或最小化。

公开(公告)号：US11034793B2

公开(公告)日：2021-06-15

申请号：US15273155

申请日：2016-09-22

Applicant: Genentech, Inc.

Inventor： Ganesh Prasadh Vissvesvaran ,  Robert David Kiss ,  Steven J. Meier ,  Inchan Kwon ,  Kara Calhoun ,  Kate Winchester ,  Amelia Adams ,  Marion Glenn ,  Stefan Koenig ,  Alan Deese

IPC: C08G65/30 ,  C12N5/00 ,  C07K16/00

Abstract: Provided herein are methods of preparing a poloxamer for use in a cell culture medium. Also provided herein are cell culture media containing the poloxamer prepared by the methods herein, as well as methods of using the media for cell culturing and polypeptide production from cells.

公开(公告)号：US11912823B2

公开(公告)日：2024-02-27

申请号：US17319886

申请日：2021-05-13

Applicant: Genentech, Inc.

Inventor： Ganesh Prasadh Vissvesvaran ,  Robert David Kiss ,  Steven J. Meier ,  Inchan Kwon ,  Kara Calhoun ,  Kate Winchester ,  Amelia Adams ,  Marion Glenn ,  Stefan Koenig ,  Alan Deese

IPC: C08G65/30 ,  C12N5/00 ,  C07K16/00

CPC classification number: C08G65/30 ,  C07K16/00 ,  C12N5/0018 ,  C07K2317/14 ,  C08G2650/50 ,  C12N2500/50

Abstract: Provided herein are methods of preparing a poloxamer for use in a cell culture medium. Also provided herein are cell culture media containing the poloxamer prepared by the methods herein, as well as methods of using the media for cell culturing and polypeptide production from cells.