公开(公告)号：US20190187126A1

公开(公告)日：2019-06-20

申请号：US15848302

申请日：2017-12-20

申请人： General Electric Company ,  TOKITAE LLC

发明人： Matthew Jeremiah MISNER ,  Gregory Andrew GROSSMANN ,  Cathryn Ellen OLSEN ,  John Richard NELSON ,  David Roger MOORE ,  Paul Michael SMIGELSKI, JR. ,  John Thomas CONNELLY ,  Benjamin David GRANT ,  Bernhard Hans WEIGL

IPC分类号： G01N33/493 ,  C07K1/22 ,  B01D15/08 ,  G01N33/50 ,  G01N33/569 ,  B01D63/02 ,  C12M1/26 ,  A61B10/00

CPC分类号： G01N33/493 ,  A61B10/007 ,  A61M2202/0028 ,  A61M2202/005 ,  A61M2202/0071 ,  A61M2202/0496 ,  B01D15/08 ,  B01D63/02 ,  C07K1/22 ,  C12M33/14 ,  G01N33/5038 ,  G01N33/558 ,  G01N33/569 ,  G01N33/5695 ,  G01N2015/1493

摘要： A device for rapid detection of a tuberculosis lipoarabinomannan (TB-LAM) is provided. The device includes a pre-concentrator unit for concentrating the TB-LAM comprising: an ion-exchange medium comprising one or more ligands configured to capture the TB-LAM from the source biological sample, wherein the captured-TB-LAM is eluted from the ion-exchange medium as an eluate comprising a concentrated form of TB-LAM; a cassette; a lateral flow assay unit disposed in the cassette; and an integration unit attached to the pre-concentrator unit and the cassette. The integration unit is configured to operatively couple and de-couple the pre-concentrator unit and the cassette. The pre-concentrator unit and the lateral flow assay unit disposed in the cassette are in a fluidic communication in a coupled form. The device for rapid detection of TB-LAM further comprises a dilutor unit.

公开(公告)号：US20180327811A1

公开(公告)日：2018-11-15

申请号：US16042320

申请日：2018-07-23

申请人： General Electric Company

发明人： Patrick McCoy SPOONER ,  Peter James TATNELL ,  Jeffrey Kenneth HORTON ,  John Richard NELSON ,  Michael John GERDES ,  Suzana KIEL ,  Ralf LENIGK ,  Alexander SCHENK ,  Wei SUN ,  Thomas HANSEN

IPC分类号： C12Q1/6804 ,  C12Q1/6869 ,  C12Q1/6876 ,  C07K16/44 ,  C12N15/11

CPC分类号： C12Q1/6804 ,  C07K16/44 ,  C12N15/11 ,  C12N2310/16 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q2522/101

摘要： The invention generally relates to methods and kits for capturing sperm nucleic acids from or in a biological sample. In one embodiment the method the method comprises, contacting the sample with a lysis solution, having a protamine-DNA complex, to lyse the cell and applying a protamine-specific binding element. This results in the protamine-specific binding element binding to the protamine-DNA to form a complex which may be captured, purified, or detected. Also provided are kits for carrying out the disclosed methods.

公开(公告)号：US20190048379A1

公开(公告)日：2019-02-14

申请号：US15631510

申请日：2017-08-11

申请人： General Electric Company

发明人： Erik Leeming KVAM ,  John Richard NELSON ,  Wei GAO

IPC分类号： C12P21/00 ,  C12P19/34

摘要： Methods for in vitro transcription and translation using a double-stranded concatemeric DNA in a eukaryotic cell-free expression system are provided. The method includes the steps of (a) contacting a double-stranded concatemeric DNA with a eukaryotic cell-free expression system, and (b) expressing a protein in vitro from the double-stranded concatemeric DNA in the eukaryotic cell-free expression system. The double-stranded concatemeric DNA includes a plurality of tandem repeat sequences. The plurality of tandem repeat sequences includes an expression sequence including a promoter, a cap-independent translation element (CITE), and an open reading frame. A final concentration of the double-stranded concatemeric DNA in the eukaryotic cell-free expression system is in a range from about 0.1 ng/μL to about 35 ng/μL. A RCA product DNA may be used as the double stranded concatemer DNA for the methods.