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公开(公告)号：US20070004041A1
公开(公告)日：2007-01-04
申请号：US11331927
申请日：2006-01-12
申请人： George Church , Brian Baynes , Edmund Pitcher
发明人： George Church , Brian Baynes , Edmund Pitcher
IPC分类号： C12N15/74
CPC分类号： C12N15/8201 , A01H1/06 , C12N15/102 , C12N15/1031 , C12N15/1058 , C12N15/1079 , C12N15/1082 , C12N15/66 , C12N15/67 , C12N15/8265 , C12N15/90
摘要： The present invention provides recombination based methods for assembling nucleic acids. In certain aspects the present invention provides hierarchical assembly methods for producing genome sized polynucleotide constructs. The methods may be used for assembling large polynucleotide constructs, for synthesizing synthetic genomes, or for introducing a plurality of nucleotide changes throughout the genome of an organism. In another aspect, the invention provides cells having increased genomic stability. For example, cells comprising alterations in at least a substantial portion of the transposons in the genome are provided.
摘要翻译： 本发明提供了用于组装核酸的基于重组的方法。 在某些方面，本发明提供了用于产生基因组大小的多核苷酸构建体的分层装配方法。 该方法可用于组装大型多核苷酸构建体，用于合成合成基因组，或用于在生物体的整个基因组中引入多个核苷酸变化。 另一方面，本发明提供具有增加的基因组稳定性的细胞。 例如，提供包含在基因组中的至少大部分转座子中的改变的细胞。
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公开(公告)号：US20060281113A1
公开(公告)日：2006-12-14
申请号：US11436478
申请日：2006-05-17
申请人： George Church , Edmund Pitcher
发明人： George Church , Edmund Pitcher
CPC分类号： C12N15/66 , C12N15/10 , C12N15/1093
摘要： Disclosed are methods of assembling nucleic acid constructs from component parts in a manner that is not dependent on the sequence of the component parts. The methods may be used to assemble large nucleic acid constructs from multiple component parts in one or more reactions. The methods may also be used to assemble two or more nucleic acid constructs in the same reaction mixture. In exemplary embodiments, the methods involve formation of a Holliday junction or bridge structure using a junction oligonucleotide.
摘要翻译： 公开了以不依赖于组分部分序列的方式从组分部分组装核酸构建体的方法。 该方法可用于在一个或多个反应中从多个组分部分组装大的核酸构建体。 该方法还可用于在相同的反应混合物中组装两个或多个核酸构建体。 在示例性实施方案中，所述方法包括使用连接寡核苷酸形成Holliday结或桥结构。
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