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    Heirarchical assembly methods for genome engineering
    1.
    发明申请
    Heirarchical assembly methods for genome engineering 审中-公开
    基因工程的组织方法

    公开(公告)号：US20070004041A1

    公开(公告)日：2007-01-04

    申请号：US11331927

    申请日：2006-01-12

    申请人： George Church ,  Brian Baynes ,  Edmund Pitcher

    发明人： George Church ,  Brian Baynes ,  Edmund Pitcher

    IPC分类号： C12N15/74

    CPC分类号： C12N15/8201 ,  A01H1/06 ,  C12N15/102 ,  C12N15/1031 ,  C12N15/1058 ,  C12N15/1079 ,  C12N15/1082 ,  C12N15/66 ,  C12N15/67 ,  C12N15/8265 ,  C12N15/90

    摘要： The present invention provides recombination based methods for assembling nucleic acids. In certain aspects the present invention provides hierarchical assembly methods for producing genome sized polynucleotide constructs. The methods may be used for assembling large polynucleotide constructs, for synthesizing synthetic genomes, or for introducing a plurality of nucleotide changes throughout the genome of an organism. In another aspect, the invention provides cells having increased genomic stability. For example, cells comprising alterations in at least a substantial portion of the transposons in the genome are provided.

    摘要翻译： 本发明提供了用于组装核酸的基于重组的方法。 在某些方面，本发明提供了用于产生基因组大小的多核苷酸构建体的分层装配方法。 该方法可用于组装大型多核苷酸构建体，用于合成合成基因组，或用于在生物体的整个基因组中引入多个核苷酸变化。 另一方面，本发明提供具有增加的基因组稳定性的细胞。 例如，提供包含在基因组中的至少大部分转座子中的改变的细胞。

    Accessible polynucleotide libraries and methods of use thereof
    2.
    发明申请
    Accessible polynucleotide libraries and methods of use thereof 审中-公开
    可接近的多核苷酸文库及其使用方法

    公开(公告)号：US20060281113A1

    公开(公告)日：2006-12-14

    申请号：US11436478

    申请日：2006-05-17

    申请人： George Church ,  Edmund Pitcher

    发明人： George Church ,  Edmund Pitcher

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12M1/34

    CPC分类号： C12N15/66 ,  C12N15/10 ,  C12N15/1093

    摘要： Disclosed are methods of assembling nucleic acid constructs from component parts in a manner that is not dependent on the sequence of the component parts. The methods may be used to assemble large nucleic acid constructs from multiple component parts in one or more reactions. The methods may also be used to assemble two or more nucleic acid constructs in the same reaction mixture. In exemplary embodiments, the methods involve formation of a Holliday junction or bridge structure using a junction oligonucleotide.

    摘要翻译： 公开了以不依赖于组分部分序列的方式从组分部分组装核酸构建体的方法。 该方法可用于在一个或多个反应中从多个组分部分组装大的核酸构建体。 该方法还可用于在相同的反应混合物中组装两个或多个核酸构建体。 在示例性实施方案中，所述方法包括使用连接寡核苷酸形成Holliday结或桥结构。

    Compositions and Methods for the Regulation of Multiple Genes of Interest in a Cell
    5.
    发明申请
    Compositions and Methods for the Regulation of Multiple Genes of Interest in a Cell 审中-公开
    用于调节细胞中多种感兴趣基因的组合物和方法

    公开(公告)号：US20120315670A1

    公开(公告)日：2012-12-13

    申请号：US13505376

    申请日：2010-11-02

    申请人： Joseph Jacobson ,  George Church

    发明人： Joseph Jacobson ,  George Church

    IPC分类号： C12P21/00 ,  C12N15/63

    CPC分类号： C12N15/1051 ,  C12N15/63

    摘要： Methods and compositions are provided for manipulating the genome of host cell to produce at least one exogenous gene product. Also provided are methods and composition for producing a programmable cell comprising a plurality of exogenous genes, wherein each exogenous gene is under the control of a disrupted regulatory sequence and wherein the disrupted regulatory sequences are restored by in vivo recombination. Preferably, the gene of interest is under the control of a genetically altered promoter which sequence recombination effects the expression of the exogenous gene(s).

    摘要翻译： 提供了用于操纵宿主细胞基因组以产生至少一种外源基因产物的方法和组合物。 还提供了用于生产包含多个外源基因的可编程细胞的方法和组合物，其中每个外源基因处于破坏的调节序列的控制下，并且其中破坏的调节序列通过体内重组恢复。 优选地，感兴趣的基因在遗传改变的启动子的控制下，该序列重组影响外源基因的表达。

    CHARACTERIZATION OF INDIVIDUAL POLYMER MOLECULES BASED ON MONOMER-INTERFACE INTERACTIONS
    6.
    发明申请
    CHARACTERIZATION OF INDIVIDUAL POLYMER MOLECULES BASED ON MONOMER-INTERFACE INTERACTIONS 审中-公开
    基于单体界面相互作用的个体聚合物分子的表征

    公开(公告)号：US20120160687A1

    公开(公告)日：2012-06-28

    申请号：US13186966

    申请日：2011-07-20

    申请人： Mark Akeson ,  Daniel Branton ,  George Church ,  David W. Deamer

    发明人： Mark Akeson ,  Daniel Branton ,  George Church ,  David W. Deamer

    IPC分类号： C07K1/26

    CPC分类号： C12Q1/6869 ,  G01N27/447 ,  G01N33/48721 ,  C12Q2565/631 ,  C12Q2565/607

    摘要： The invention relates to a method for detecting a double-stranded region in a nucleic acid by (1) providing two separate, adjacent pools of a medium and a interface between the two pools, the interface having a channel so dimensioned as to allow sequential monomer-by-monomer passage of a single-stranded nucleic acid, but not of a double-stranded nucleic acid, from one pool to the other pool; (2) placing a nucleic acid polymer in one of the two pools; and (3) taking measurements as each of the nucleotide monomers of the single-stranded nucleic acid polymer passes through the channel so as to differentiate between nucleotide monomers that are hybridized to another nucleotide monomer before entering the channel and nucleotide monomers that are not hybridized to another nucleotide monomer before entering the channel.

    摘要翻译： 本发明涉及一种用于通过（1）在两个池之间提供两个独立的相邻的介质池和界面来检测核酸中的双链区域的方法，所述界面具有如此尺寸以允许顺序单体 单链核酸但不是双链核酸的单体通过从一个池到另一个池; （2）将核酸聚合物置于两个池中的一个中; 和（3）测量，因为单链核酸聚合物的每个核苷酸单体通过通道，以便在进入通道之前与另一个核苷酸单体杂交的核苷酸单体与未杂交的核苷酸单体区分 进入通道之前的另一种核苷酸单体。

    Methods for assembly of high fidelity synthetic polynucleotides
    10.
    发明申请
    Methods for assembly of high fidelity synthetic polynucleotides 审中-公开
    装配高保真合成多核苷酸的方法

    公开(公告)号：US20060194214A1

    公开(公告)日：2006-08-31

    申请号：US11068321

    申请日：2005-02-28

    申请人： George Church ,  Joseph Jacobson ,  Brian Baynes

    发明人： George Church ,  Joseph Jacobson ,  Brian Baynes

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

    CPC分类号： C12Q1/6813 ,  C12Q2523/305 ,  C12Q2525/131 ,  C12Q2525/155

    摘要： Disclosed are methods of manufacturing synthetic DNAs, that is, DNAs made at least in significant part by chemical synthesis of polynucleotide polymers. Also provided are methods for assembling plural DNAs in the same pool by multiplexed assembly of synthetic oligonucleotides. In exemplary embodiments, the methods involve pre-amplification of one or more oligonucleotides using “universal” primers, reduction of the error rate in oligonucleotide and/or polynucleotide products, and sequence optimization and oligonucleotides design.

    摘要翻译： 公开了制造合成DNA的方法，即通过化学合成多核苷酸聚合物至少部分制备的DNA。 还提供了通过合成寡核苷酸的多重组装在同一池中组装多个DNA的方法。 在示例性实施方案中，所述方法包括使用“通用”引物预扩增一个或多个寡核苷酸，降低寡核苷酸和/或多核苷酸产物中的错误率，以及序列优化和寡核苷酸设计。