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公开(公告)号：US20120108459A1
公开(公告)日：2012-05-03
申请号：US13279302
申请日：2011-10-23
IPC分类号： C40B30/04
摘要： The present invention is directed to devices and methods for molecular association, particularly to devices and methods for hybridization of nucleic acids utilizing temperature gradients and imaging thereof. In one aspect, a molecular hybridization system generally includes a substrate having a plurality of molecular probes attached thereto, the plurality of probes being generally present in multiple copies arranged in localized formations on the surface of the substrate. The molecular hybridization system further generally includes a chamber that encloses the plurality of molecular probes such that a fluid containing sample may be applied and kept in contact with the substrate having the probes thereon. The molecular hybridization system also includes a temperature affecting system that generally produces at least one desired temperature on the surface of the substrate and in the adjacent fluid within the chamber.
摘要翻译： 本发明涉及用于分子缔合的装置和方法，特别涉及利用温度梯度和其成像对核酸进行杂交的装置和方法。 一方面，分子杂交系统通常包括具有附着于其上的多个分子探针的基底，所述多个探针通常存在于基片的表面上以局部化形式布置的多个拷贝。 分子杂交系统还通常包括包围多个分子探针的室，使得含有流体的样品可以被施加并保持与其上具有探针的基底接触。 分子杂交系统还包括温度影响系统，其通常在衬底的表面和室内的相邻流体中产生至少一个期望的温度。
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公开(公告)号：US20090099045A1
公开(公告)日：2009-04-16
申请号：US12249525
申请日：2008-10-10
摘要： The present invention is directed to devices and methods for molecular association, particularly to devices and methods for hybridization of nucleic acids utilizing temperature gradients and imaging thereof. In one aspect, a molecular hybridization system generally includes a substrate having a plurality of molecular probes attached thereto, the plurality of probes being generally present in multiple copies arranged in localized formations on the surface of the substrate. The molecular hybridization system further generally includes a chamber that encloses the plurality of molecular probes such that a fluid containing sample may be applied and kept in contact with the substrate having the probes thereon. The molecular hybridization system also includes a temperature affecting system that generally produces at least one desired temperature on the surface of the substrate and in the adjacent fluid within the chamber.
摘要翻译： 本发明涉及用于分子缔合的装置和方法，特别涉及利用温度梯度和其成像对核酸进行杂交的装置和方法。 一方面，分子杂交系统通常包括具有附着于其上的多个分子探针的基底，多个探针通常以多个拷贝布置在基底表面上的局部形成中。 分子杂交系统还通常包括包围多个分子探针的室，使得含有流体的样品可以被施加并保持与其上具有探针的基底接触。 分子杂交系统还包括温度影响系统，其通常在衬底的表面和室内的相邻流体中产生至少一个期望的温度。
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公开(公告)号：US20100087336A1
公开(公告)日：2010-04-08
申请号：US12558523
申请日：2009-09-12
申请人： George Jackson , Roger McNichols , Ulrich Strych , George E. Fox , Victor G. Stepanov , Yamei Liu
发明人： George Jackson , Roger McNichols , Ulrich Strych , George E. Fox , Victor G. Stepanov , Yamei Liu
CPC分类号： G01N33/5308
摘要： The present invention relates to methods of generating amounts of selective nucleic acids. The present invention further relates to selective nucleic acids incorporated within non-coding nucleic acids, capable of binding to or altering a target molecule. Selective nucleic acids may generally refer to, but are not limited to, deoxyribonucleic acids (DNAs), ribonucleic acids (RNAs), artificially modified nucleic acids, combinations or modifications thereof. Selective nucleic acids may also generally refer to, but are not limited to, nucleic acid aptamers, aptazymes, ribozymes, deoxyribozymes, nucleic acid probes, small interfering RNAs (siRNAs), micro RNAs (miRNAs), short hairpin RNAs (shRNAs), antisense nucleic acids, diagnostic probes or probe libraries, aptamer inhibitors, precursors of any of the above and/or combinations or modifications thereof. In one aspect, a method for generating amounts of selective nucleic acids includes incorporating a selective nucleic acid sequence into a carrier nucleic acid. In general, the carrier nucleic acid may be transcribed by a cell into a product nucleic acid which may carry an incorporated selective nucleic acid sequence.
摘要翻译： 本发明涉及产生选择性核酸量的方法。 本发明还涉及掺入非编码核酸中的能够结合或改变靶分子的选择性核酸。 选择性核酸通常可以指但不限于脱氧核糖核酸（DNA），核糖核酸（RNA），人造修饰的核酸，其组合或修饰。 选择性核酸还可以通常指但不限于核酸适体，适体，核酶，脱氧核酶，核酸探针，小干扰RNA（siRNA），微RNA（miRNA），短发夹RNA（shRNA），反义 核酸，诊断探针或探针文库，适体抑制剂，上述任一种的前体和/或其组合或修饰。 一方面，产生选择性核酸量的方法包括将选择性核酸序列掺入载体核酸中。 通常，载体核酸可以被细胞转录成可以携带掺入的选择性核酸序列的产物核酸。
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公开(公告)号：US08314052B2
公开(公告)日：2012-11-20
申请号：US12683429
申请日：2010-01-07
申请人： George Jackson
发明人： George Jackson
CPC分类号： C12Q1/6837 , C07K1/22
摘要： The present invention relates to methods for generating functional biomolecules. In one exemplary aspect of the invention, generation of functional biomolecules may be performed against multiple targets simultaneously within a single system. In general, a plurality of targets may be disposed within in a single reaction volume and a library of biomolecules, such as a nucleic acid library, may be applied to the reaction volume. The members of the library that do not bind to any of the plurality of targets under given conditions may then be partitioned. The remaining members of the library may then be marked and/or tagged, such as to identify the particular target or targets to which the member of the library binds. The binding members of the library may then be isolated and, by virtue of the marking or tagging, be matched to a particular target or targets.
摘要翻译： 本发明涉及产生功能性生物分子的方法。 在本发明的一个示例性方面，功能性生物分子的产生可以在单个系统内同时针对多个靶标进行。 通常，多个靶可以设置在单个反应体积内，并且生物分子文库（例如核酸文库）可以应用于反应体积。 在给定条件下不绑定到任何多个目标的库的成员可以被划分。 然后可以标记和/或标记图书馆的剩余成员，例如识别图书馆成员所绑定的特定目标或目标。 然后可以分离图书馆的约束成员，并且通过标记或标记来匹配特定的目标或目标。
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公开(公告)号：US08034569B2
公开(公告)日：2011-10-11
申请号：US12479806
申请日：2009-06-06
申请人： George Jackson , Christopher Quan
发明人： George Jackson , Christopher Quan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6804 , C12Q1/6844 , C12Q2525/205 , C12Q2537/161 , G01N33/5308 , C12Q2537/163 , C12Q2525/207
摘要： This invention relates to methods for molecular detection, particularly to methods utilizing target-specific molecular probes. In exemplary embodiments, target-specific molecular probes include single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) aptamers. In general, the molecular probe may bind with relatively high specificity to a given target. In one aspect, a method for molecular detection comprises a molecular probe paired to a reporter molecule wherein the molecular probe impairs the amplification of the reporter molecule in the absence of the target molecule.
摘要翻译： 本发明涉及分子检测方法，特别涉及利用靶特异性分子探针的方法。 在示例性实施方案中，靶特异性分子探针包括单链脱氧核糖核酸（DNA）或核糖核酸（RNA）适体。 通常，分子探针可以以相对高的特异性结合给定的靶。 在一个方面，用于分子检测的方法包括与报告分子配对的分子探针，其中分子探针在不存在靶分子的情况下损害了报告分子的扩增。
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公开(公告)号：US20090148493A1
公开(公告)日：2009-06-11
申请号：US12329527
申请日：2008-12-05
申请人： Ralph Ballerstadt , George Jackson
发明人： Ralph Ballerstadt , George Jackson
IPC分类号： A61F2/00 , B05D1/00 , A61K31/7088 , A61K31/7105 , A61K31/711
CPC分类号： A61L27/34 , A61K31/7088 , A61K31/7105 , A61K31/711 , A61L2400/18
摘要： The present invention is directed to devices with enhanced biocompatibility and methods for generating and utilizing such devices. The present invention is further directed to enhanced biocompatibility utilizing oligonucleotide functionalization. In one aspect, a device for implantation and/or prolonged exposure to the body tissues includes a functionalized surface. The functionalized surface generally enhances the biocompatibility of the device with body tissues. In some embodiments, the functionalized surface includes substances for controlling interaction between the device and the body tissues. Substances for controlling interactions may include, but are not limited to, polymeric materials, biomolecules, ions and/or ion-releasing substances, and/or any other appropriate substance or combination thereof. In exemplary embodiments, the functionalized surface includes oligonucleotides for controlling interaction between the device and the body tissues. In some exemplary embodiments, the oligonucleotides are aptamers.
摘要翻译： 本发明涉及具有增强的生物相容性的装置和用于产生和利用这些装置的方法。 本发明进一步涉及利用寡核苷酸功能化的增强的生物相容性。 一方面，用于植入和/或延长暴露于身体组织的装置包括功能化表面。 功能化表面通常增强了与身体组织的装置的生物相容性。 在一些实施方案中，功能化表面包括用于控制装置和身体组织之间的相互作用的物质。 用于控制相互作用的物质可以包括但不限于聚合物材料，生物分子，离子和/或离子释放物质，和/或任何其它合适的物质或其组合。 在示例性实施方案中，功能化表面包括用于控制装置和身体组织之间的相互作用的寡核苷酸。 在一些示例性实施方案中，寡核苷酸是适体。
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公开(公告)号：US20060136350A1
公开(公告)日：2006-06-22
申请号：US10898212
申请日：2004-11-26
申请人： George Jackson
发明人： George Jackson
CPC分类号： G06F16/00
摘要： Process for space time detection with the past, present and future using factual real life objects, actions and events allowing for desired actions. The process can run on any chosen computer system or software using file or database formats containing the object information or can be processed with human intellect without computers. Information is entered into a file or database system with automated or manual methods storing the date and time of the objects being people, places and things. The objects locations and surroundings are mapped and stored. Information about the objects are obtained and if not on file they are entered. A recognition process is then used to decode variations of the object information and written to storage. As each record is stored an automated monitoring, reporting alarm and analysis process is then available for human or computer analysis allowing for any preprogrammed or chosen action.
摘要翻译： 使用过去，现在和将来的空间时间检测过程，使用事实的现实生活对象，允许所需操作的动作和事件。 该过程可以在任何选定的计算机系统或软件上运行，使用包含对象信息的文件或数据库格式，或者可以在没有计算机的情况下用人类智力进行处理。 信息以自动或手动方式输入到文件或数据库系统中，将对象的日期和时间存储为人员，地点和事物。 映射和存储对象位置和周围环境。 获取有关对象的信息，如果不是文件，则输入它们。 然后使用识别过程来解码对象信息的变化并写入存储。 随着每个记录的存储，自动监控，报告报警和分析过程随后可用于人或计算机分析，允许任何预编程或选择的操作。
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公开(公告)号：US20050071936A1
公开(公告)日：2005-04-07
申请号：US10743751
申请日：2003-12-24
申请人： George Jackson
发明人： George Jackson
CPC分类号： E01D21/00
摘要： A bridge overhang bracket includes an elongate top member having inner and outer ends an elongate diagonal member having an inner end and an outer end, the outer end of the diagonal member pivotally attached to the outer end of the top member, upper and lower pivot joints secured to the top member and diagonal member, respectively, adjacent the respective inner ends thereof, and an elongate side member extending between and engaged with the upper and lower pivot joints. At least one of the pivot joints comprises an adjustable axial engagement mechanism for adjusting the position along the length of the side member at which the at least one pivot joint engages the side member.
摘要翻译： 桥式悬挂支架包括细长的顶部构件，其具有内部和外部端部，具有内端和外端的细长对角构件，对角构件的外端可枢转地附接到顶部构件的外端，上部和下部枢转接头 分别固定到顶部构件和对角构件，邻近其相应的内端，以及在上部和下部枢转接头之间延伸并与其接合的细长侧部构件。 枢转接头中的至少一个包括可调节的轴向接合机构，用于调节沿着侧部件的长度的位置，在该位置处，至少一个枢转接头接合侧部件。
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公开(公告)号：US4106757A
公开(公告)日：1978-08-15
申请号：US765767
申请日：1977-02-04
申请人： George Jackson
发明人： George Jackson
CPC分类号： C21D9/63
摘要： A furnace for continuously heat treating metallic strip comprises a substantially flat horizontal entry floatation table having a trailing portion which inclines downwardly towards the base of a floatation trough. The trough extends along substantially the full length of the heating zone furnace. An exit table includes a leading portion which inclines upwardly from the base of the trough to a generally horizontal flat floatation surface. Entry and exit seals are positioned above the entry and exit tables respectively.
摘要翻译： 用于连续热处理金属条的炉包括一个基本平坦的水平入口漂浮台，其具有向下朝向浮动槽的底部倾斜的后部。 槽基本上在加热区炉的整个长度上延伸。 出口台包括从槽的底部向上倾斜到大致水平的平坦浮动表面的引导部分。 入口和出口密封件分别位于入口和出口表面上方。
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公开(公告)号：US20110306054A1
公开(公告)日：2011-12-15
申请号：US13212136
申请日：2011-08-17
申请人： George Jackson , Christopher Quan
发明人： George Jackson , Christopher Quan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6804 , C12Q1/6844 , C12Q2525/205 , C12Q2537/161 , G01N33/5308 , C12Q2537/163 , C12Q2525/207
摘要： This invention relates to methods for molecular detection, particularly to methods utilizing target-specific molecular probes. In exemplary embodiments, target-specific molecular probes include single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) aptamers. In general, the molecular probe may bind with relatively high specificity to a given target. In one aspect, a method for molecular detection comprises a molecular probe paired to a reporter molecule wherein the molecular probe impairs the amplification of the reporter molecule in the absence of the target molecule.
摘要翻译： 本发明涉及用于分子检测的方法，特别涉及利用靶特异性分子探针的方法。 在示例性实施方案中，靶特异性分子探针包括单链脱氧核糖核酸（DNA）或核糖核酸（RNA）适体。 通常，分子探针可以以相对高的特异性结合给定的靶。 在一个方面，用于分子检测的方法包括与报告分子配对的分子探针，其中分子探针在不存在靶分子的情况下损害了报告分子的扩增。
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