公开(公告)号：US11723553B2

公开(公告)日：2023-08-15

申请号：US16788035

申请日：2020-02-11

申请人： Giner, Inc.

发明人： Badawi M. Dweik ,  Avni A. Argun ,  Anahita Karimi

IPC分类号： A61B5/097 ,  G01N27/327 ,  A61B5/08 ,  A61B5/18 ,  A61K31/352 ,  G01N27/30 ,  G01N27/333

CPC分类号： A61B5/082 ,  A61B5/18 ,  A61K31/352 ,  G01N27/308 ,  G01N27/327 ,  G01N27/3335

摘要： Method and system for detecting and/or quantifying Δ9-tetrahydrocannibinol (THC) in exhaled breath. In one embodiment, the method involves providing an electrochemical sensing element, the electrochemical sensing element including a working electrode, and also providing a filter that traps THC in exhaled breath. Next, a subject exhales onto the filter, whereby at least some of the THC, if present, is trapped in the filter. Next, the filter is washed with an eluent, whereby at least some of the THC trapped in the filter is eluted in an eluate. Next, the eluate is deposited onto the working electrode of the electrochemical sensing element, and the eluate is dried, whereby any THC present is immobilized on the working electrode. Next, an electrolytic solution is delivered to the electrochemical sensing element, and the THC immobilized on the working electrode is directly electrochemically detected and/or quantified using a pulse voltammetry technique, such as square-wave voltammetry.

公开(公告)号：US11092585B2

公开(公告)日：2021-08-17

申请号：US16287431

申请日：2019-02-27

申请人： Giner, Inc.

发明人： Badawi M. Dweik ,  Avni A. Argun ,  Katherine E. Harrison

IPC分类号： G01N33/18 ,  G01N27/327 ,  G01N27/42

摘要： Method and system for detection and quantification of oxidizable organics in water. The method involves the partial electrolytic decomposition of the oxidizable organics in a short time frame, preferably less than five seconds, and does not involve the use of toxic reagents. The system includes an electrochemical sensor probe that, in turn, includes a boron-doped diamond microelectrode array. The system additionally includes an electronic transducer and a computing device. The system utilizes an analysis technique to convert sensor signal to a result that can be correlated with COD or BOD values obtained by standard methods. The method and system are particularly suitable for, but not limited to, use in monitoring of water quality at wastewater treatment plants. By employing the method before and after adding aerobic microorganisms to the sample, the method may be used to distinguish biologically oxidizable organics from total oxidizable organics.

公开(公告)号：US20230374570A1

公开(公告)日：2023-11-23

申请号：US18295613

申请日：2023-04-04

申请人： Giner, Inc.

发明人： Avni A. Argun ,  Muhit Rana

IPC分类号： C12Q1/6834 ,  C12Q1/04

CPC分类号： C12Q1/6834 ,  C12Q1/04

摘要： Method and system for detecting a fungal gene and kit for use with same. According to one embodiment, a nucleotide capture probe is coupled to a surface, such as a well of a 96-well plate, using a biotin-streptavidin interaction. The capture probe is preferably specific for a portion of a target sequence of a fungal gene of interest. Upon capture, additional portions of the target sequence of the fungal gene of interest are tagged with nucleotide labeling probes. An enzyme label, which is preferably a poly-horseradish peroxidase conjugate, is then attached to each of the labeling probes, for example, by a biotin-streptavidin interaction. The enzyme label catalyzes the oxidation of a substrate, such as 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide. The oxidized substrate may then be detected photonically, by visually detecting a colorimetric change or by absorbance readings, and/or detected electrochemically in the presence of an acid.

公开(公告)号：US20230048049A1

公开(公告)日：2023-02-16

申请号：US17887347

申请日：2022-08-12

申请人： Giner, Inc.

发明人： Avni A. Argun ,  Muhit Rana ,  Badawi M. Dweik ,  Niazul I. Khan ,  Andrew Weber

IPC分类号： G01N27/06 ,  G01N33/94 ,  G01N33/543

摘要： Method and system for detecting drug and/or drug metabolites in a liquid sample, such as a wastewater sample. According to one embodiment, the method involves providing a device that includes a graphene field effect transistor and a first aptamer coupled to the graphene field effect transistor in a first well, the first aptamer being selective for a first drug or drug metabolite. Next, a liquid sample is introduced to the first aptamer of the device. Next, a sweeping liquid gate voltage is applied to the device to obtain a resistance versus liquid gate voltage plot for the device. Next, the Dirac voltage shift, if any, in the liquid gate voltage plot for the device is used to determine the presence and/or quantity of the drug or drug metabolite. Additional aptamers selective for different drugs or drug metabolites of interest may also be included in other wells of the device.

公开(公告)号：US20240288452A1

公开(公告)日：2024-08-29

申请号：US18590417

申请日：2024-02-28

申请人： Giner, Inc.

发明人： Avni A. Argun ,  Muhit Rana

IPC分类号： G01N33/74 ,  G01N27/327 ,  G01N33/53 ,  G01N33/543

CPC分类号： G01N33/74 ,  G01N27/3276 ,  G01N27/3277 ,  G01N33/5308 ,  G01N33/5438

摘要： Method and kit for detecting oxytocin in a biological sample. According to one embodiment, the method involves providing a test sample. The test sample is prepared by obtaining a biological sample from one or more subjects and then diluting the biological sample with 1×PBS (phosphate-buffered saline) and 2 mM MgCl2, pH 7.4. The method also involves providing a test cassette. The test cassette includes an electrochemical sensing element and a capture element. The capture element is coupled to the electrochemical sensing element, and the capture element has a binding affinity and specificity for oxytocin. The test sample is then added to the test cassette, whereby at least some of the oxytocin present in the test sample binds to the capture element. Then, the test cassette is electrochemically analyzed for any captured oxytocin,

公开(公告)号：US20200025740A1

公开(公告)日：2020-01-23

申请号：US16287431

申请日：2019-02-27

申请人： Giner, Inc.

发明人： Badawi M. Dweik ,  Avni A. Argun ,  Katherine E. Harrison

IPC分类号： G01N33/18 ,  G01N27/327 ,  G01N27/42

摘要： Method and system for detection and quantification of oxidizable organics in water. The method involves the partial electrolytic decomposition of the oxidizable organics in a short time frame, preferably less than five seconds, and does not involve the use of toxic reagents. The system includes an electrochemical sensor probe that, in turn, includes a boron-doped diamond microelectrode array. The system additionally includes an electronic transducer and a computing device. The system utilizes an analysis technique to convert sensor signal to a result that can be correlated with COD or BOD values obtained by standard methods. The method and system are particularly suitable for, but not limited to, use in monitoring of water quality at wastewater treatment plants. By employing the method before and after adding aerobic microorganisms to the sample, the method may be used to distinguish biologically oxidizable organics from total oxidizable organics.