公开(公告)号：US20240200019A1

公开(公告)日：2024-06-20

申请号：US18288954

申请日：2022-04-28

申请人： Ginkgo Bioworks, Inc.

发明人： Annapurna Kamineni ,  Arthur J. Shaw ,  Vasiliki Tsakraklides

IPC分类号： C12N1/16 ,  C12N9/10 ,  C12N9/88 ,  C12N15/81 ,  C12P5/00 ,  C12P7/6454 ,  C12P7/6463 ,  C12R1/645

CPC分类号： C12N1/165 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/815 ,  C12P5/007 ,  C12P7/6454 ,  C12P7/6463 ,  C07K2319/00 ,  C12R2001/645 ,  C12Y203/01008 ,  C12Y401/02009

摘要： Aspects of the disclosure are directed to genetically modified yeast cells and methods for use. Certain aspects are directed to recombinant yeast cells comprising exogenous nucleic acid sequences encoding phosphotransacetylase and/or phosphoketolase proteins, including a phosphoketolase protein from Clostridium acetobutylicum. Also disclosed are methods for generating recombinant yeast cells and methods of use of such cells for production of one or more products, including lipids, oils, fatty acids, and triacylglycerides.

公开(公告)号：US20240084340A1

公开(公告)日：2024-03-14

申请号：US18227147

申请日：2023-07-27

申请人： Ginkgo Bioworks, Inc.

发明人： Arthur J. Shaw ,  Hannah Blitzblau ,  Donald V. Crabtree

IPC分类号： C12P7/6409 ,  A23D9/02 ,  C11B1/10 ,  C11C3/00 ,  C12N9/02 ,  C12N9/10 ,  C12Q1/686

CPC分类号： C12P7/6409 ,  A23D9/02 ,  C11B1/10 ,  C11C3/00 ,  C12N9/001 ,  C12N9/1007 ,  C12Q1/686

摘要： Nucleic acids and cells comprising a methyltransferase gene and/or a reductase gene are disclosed. These nucleic acids and cells may be used to produce branched (methyl)lipids, such as 10-methylstearate.

公开(公告)号：US11236373B2

公开(公告)日：2022-02-01

申请号：US16648117

申请日：2018-09-20

申请人： Ginkgo Bioworks, Inc.

发明人： Arthur J. Shaw ,  Hannah Blitzblau ,  Donald V. Crabtree

IPC分类号： C12N9/00 ,  C12P7/64 ,  C12N9/02 ,  C12N9/10

摘要： Disclosed herein are cells, nucleic acids, and proteins that can be used to produce branched (methyl)lipids, such as 10-methylstearic acids, and compositions that include such lipids. Cells disclosed herein comprise methyltransferase and/or reductase genes from bacteria of the class Gammaproteobacteria, which encode enzymes capable of catalyzing the production of branched (methyl)lipids from unbranched, unsaturated lipids. Saturated branched (methyl)lipids produced using embodiments of the present invention have favorable low-temperature fluidity and favorable oxidative stability, which are desirable properties for lubricants and specialty fluids.

公开(公告)号：US20210269835A1

公开(公告)日：2021-09-02

申请号：US17197710

申请日：2021-03-10

申请人： Ginkgo Bioworks, Inc.

发明人： Arthur J. Shaw ,  Hannah Blitzblau ,  Donald V. Crabtree

IPC分类号： C12P7/64 ,  C11B1/10 ,  A23D9/02 ,  C11C3/00 ,  C12N9/02 ,  C12N9/10 ,  C12Q1/686

摘要： Nucleic acids and cells comprising a methyltransferase gene and/or a reductase gene are disclosed. These nucleic acids and cells may be used to produce branched (methyl)lipids, such as 10-methylstearate.

公开(公告)号：US20220340939A1

公开(公告)日：2022-10-27

申请号：US17649365

申请日：2022-01-30

申请人： Ginkgo Bioworks, Inc.

发明人： Arthur J. Shaw ,  Hannah Blitzblau ,  Donald V. Crabtree

IPC分类号： C12P7/6463 ,  C12N9/02 ,  C12N9/10

摘要： Disclosed herein are cells, nucleic acids, and proteins that can be used to produce branched (methyl)lipids, such as 10-methylstearic acids, and compositions that include such lipids. Cells disclosed herein comprise methyltransferase and/or reductase genes from bacteria of the class Gammaproteobacteria, which encode enzymes capable of catalyzing the production of branched (methyl)lipids from unbranched, unsaturated lipids. Saturated branched (methyl)lipids produced using embodiments of the present invention have favorable low-temperature fluidity and favorable oxidative stability, which are desirable properties for lubricants and specialty fluids.

公开(公告)号：US10975398B2

公开(公告)日：2021-04-13

申请号：US16664378

申请日：2019-10-25

申请人： Ginkgo Bioworks, Inc.

发明人： Arthur J. Shaw ,  Hannah Blitzblau ,  Donald V. Crabtree

IPC分类号： C12P7/64 ,  C11B1/10 ,  A23D9/02 ,  C11C3/00 ,  C12N9/02 ,  C12N9/10 ,  C12Q1/686

摘要： Nucleic acids and cells comprising a methyltransferase gene and/or a reductase gene are disclosed. These nucleic acids and cells may be used to produce branched (methyl)lipids, such as 10-methylstearate.