公开(公告)号：US5830134A

公开(公告)日：1998-11-03

申请号：US725912

申请日：1996-10-04

申请人： Giuseppe Caputo ,  Giampiero Porro ,  Stefano Rinaldi

发明人： Giuseppe Caputo ,  Giampiero Porro ,  Stefano Rinaldi

IPC分类号： G01N21/27 ,  A61B5/00 ,  G01N21/31

CPC分类号： G01N21/27

摘要： Physico-chemical parameters, such as pH, pO.sub.2, pCO.sub.2, % O.sub.2, sat, etc., are determined with the use of a sensitive medium, such as a chromophore, having at least one optical property the profile of the spectrum whereof over a predetermined wavelength spectrum of optical radiation varies as a function of the parameter to be determined. The profile of the spectrum is analysed, for example by means of polychromatic sensor means acting on the medium's spectrum, which is preferably substantially continuous, so as to enable the analysis to be carried out with the aid of a profile-recognition function by typical pattern-recognition operations. The determination is thus rendered largely independent of numerous perturbing factors such as those resulting from the use of partially disposable monitoring units, thus eliminating the need for calibration steps.

摘要翻译： 通过使用具有至少一种光学性质的敏感介质如发色团来确定物理化学参数，例如pH，pO2，pCO2，％O2，sat等物理化学参数， 光辐射的预定波长谱根据要确定的参数的函数而变化。 分析光谱的分布，例如通过作用在介质光谱上的多色传感器装置，其优选地基本上是连续的，以便能够借助于通过典型图案的轮廓识别功能进行分析 - 识别操作。 因此，该确定在很大程度上独立于许多扰动因素，例如由于使用部分一次性监视单元而导致的扰动因素，从而消除了对校准步骤的需要。

公开(公告)号：US06731379B2

公开(公告)日：2004-05-04

申请号：US10634169

申请日：2003-08-05

申请人： Giuseppe Caputo ,  Raffaele Castelli ,  Elena Comoglio ,  Leopoldo Della Ciana ,  Arnaldo Giannetti

发明人： Giuseppe Caputo ,  Raffaele Castelli ,  Elena Comoglio ,  Leopoldo Della Ciana ,  Arnaldo Giannetti

IPC分类号： G01N3349

CPC分类号： G01N33/5308 ,  G01N2400/40

摘要： A device for determining the concentration of heparin in a fluid sample comprising: a container for holding the fluid sample; a container for holding the dye solution; a mixer for mixing the fluid sample and the dye solution; an illumination source for illuminating a mixture comprising the fluid sample and the dye solution with electromagnetic radiation having a substantially continuous range of wavelengths in the visible range; a detector for detecting the absorption spectrum of the mixture within the substantially continuous range of wavelengths; a recorder for recording the absorption spectrum of the mixture within the substantially continuous range of wavelengths; and a calculator for calculating a spectral parameter.

摘要翻译： 一种用于确定流体样品中肝素浓度的装置，包括：用于保持流体样品的容器; 用于保持染料溶液的容器; 用于混合流体样品和染料溶液的混合器; 照明源，用于通过电磁辐射照射包含流体样品和染料溶液的混合物，所述电磁辐射具有在可见光范围内的基本上连续的波长范围; 检测器，用于在基本连续的波长范围内检测混合物的吸收光谱; 用于在基本上连续的波长范围内记录混合物的吸收光谱的记录器; 以及用于计算光谱参数的计算器。

公开(公告)号：US20050245734A1

公开(公告)日：2005-11-03

申请号：US10822442

申请日：2004-04-12

申请人： Giuseppe Caputo ,  Leopoldo Ciana

发明人： Giuseppe Caputo ,  Leopoldo Ciana

IPC分类号： C09B23/06 ,  C09B23/08 ,  C07H19/048 ,  C07D43/14

CPC分类号： C09B23/06 ,  C09B23/083

摘要： A process for preparing an asymmetrical indocyanine dye comprising the steps of: a) reacting a first quaternised indolenine or substituted indolenine with a compound of the formula (II) or hydrochloride thereof, wherein n is 0 or 1 Ph is phenyl or substituted phenyl X is hydrogen, halogen or alkyl, preferably chlorine, in a solvent selected from the group consisting of acetic acid, acetic anhydride and mixtures thereof in the presence of acetyl chloride, to obtain an intermediate hemicyanine, and b) further reacting said intermediate hemicyanine with a second quaternised indolenine or substituted indolenine different from said first indolenine.

摘要翻译： 一种制备不对称吲哚菁染料的方法，包括以下步骤：a）使第一季铵化的吲哚宁或取代的吲哚宁与式（II）化合物或其盐酸盐反应，其中n为0或1 Ph为苯基或取代的苯基X为 氢，卤素或烷基，优选氯，在乙酰氯存在下，在选自乙酸，乙酸酐及其混合物的溶剂中，得到中间体半花青碱，和b）进一步使所述中间体半花青与第二 与所述第一种吲哚宁不同的季铵化吲哚宁或取代的吲哚宁。

公开(公告)号：US06448008B1

公开(公告)日：2002-09-10

申请号：US09609035

申请日：2000-06-30

申请人： Giuseppe Caputo ,  Leopoldo Della Ciana

发明人： Giuseppe Caputo ,  Leopoldo Della Ciana

IPC分类号： C12Q168

CPC分类号： C07H21/00 ,  C07H19/04 ,  C09B23/02 ,  G01N33/533 ,  G01N33/582

摘要： A fluorescent cyanine dye of the following general formula is disclosed: wherein: X1 and X2 are independently selected from the group consisting of —O—, —S—, —C(CH3)2 or —C═CH2; Y1 and Y2 are nonmetal atoms required to form a benzo-condensed or naphtho-condensed ring; Q is a conjugated moiety that increases the fluorescent quantum yield and the stability of the compound; R1 and R2 are independently selected from the group consisting of H, C1-C4, alkyl, alkylensulfonic group or alkylensulfonate group wherein the alkylene group has from 1 to 4 carbon atoms; R3, R4 and R5 are independently selected from the group consisting of H, a sulfonic group, a sulfonate group, alkylensulfonic, alkylensulfonate and —SO2NH(CH2)m—W—(CH2)nZ, wherein alkylene has 1 to 4 carbon atoms, with the proviso that at least one of R1 to R5 contains a sulfonic or sulfonate group; W is absent or is a group selected from —SO2NH, —O—, —COO—, or —CONH—; n=0-12 and m=0-12 with the provisos that m+n≦12 and at least one of m and n≠0; and Z is, or contains a N, O or S nucleophile functionality or is, or contains a functionality capable of reacting with N, O or S nucleophiles. Nucleophile functionalities include —NH2, —OH, and —SH groups; groups capable of reacting with such functionalities include —COCl, —COOCOR, —CONHNH2, N-hydroxysuccinimido esters, —NCS, —CHO, —COCH2I, phosphoramidite and maleimido.

摘要翻译： 公开了以下通式的荧光花青染料：其中：X 1和X 2独立地选自-O - ， - S - ， - C（CH 3）2或-C = CH 2; Y 1和Y 2是非金属 需要形成苯并稠合或萘并稠环的原子; Q是增加荧光量子产率和化合物稳定性的共轭部分; R1和R2独立地选自H，C1-C4，烷基，亚烷基磺基或亚烷基磺酸酯基，其中亚烷基具有1〜 4个碳原子; R 3，R 4和R 5独立地选自H，磺酸基，磺酸基，亚烷基磺酸，亚烷基磺酸酯和-SO 2 NH（CH 2）m W-（CH 2）n Z，其中亚烷基具有1至4个碳原子， 条件是R 1至R 5中的至少一个含有磺酸基或磺酸酯基; W不存在或是选自-SO 2 NH，-O - ， - COO-或-CONH-的基团; n = 0-12且m = 0-12，条件是m + n <= 12，m和n <0中的至少一个; 且Z为或含有N，O或S亲核试剂官能团，或为或含有能够与N，O或S亲核试剂反应的官能团。 亲核体官能团包括-NH 2，-OH和-SH基团; 能够与这些官能团反应的基团包括-COCl，-COOCOR，-CONHNH 2，N-羟基琥珀酰亚胺酯，-NCS，-CHO，-COCH2I，亚磷酰胺和马来酰亚胺。

公开(公告)号：US6136612A

公开(公告)日：2000-10-24

申请号：US43767

申请日：1998-06-18

申请人： Leopoldo Della Ciana ,  Andrea Grignani ,  Mariacristina Cassullo ,  Giuseppe Caputo

发明人： Leopoldo Della Ciana ,  Andrea Grignani ,  Mariacristina Cassullo ,  Giuseppe Caputo

IPC分类号： C09B23/01 ,  C09B23/02 ,  G01N33/533 ,  C07D209/56

CPC分类号： G01N33/533 ,  C09B23/0066 ,  C09B23/02 ,  Y10S436/80 ,  Y10S436/817

摘要： The present invention is directed to fluorescent dyes and its valence tautomers of the formula wherein Q represents a conjugated moiety that increases the fluorescent quantum yield of the compound; R.sub.1 is a functionalized group of the formula--(CH.sub.2).sub.j Y, wherein Y is selected from the group consisting of SO.sub.3 H, COOH, NH.sub.2, CHO, NCS, epoxy, phthalimido, and COOZ, wherein Z represents a leaving group; R.sub.2 is a functionalized group of the formula --(CH.sub.2).sub.k Y', wherein Y' is selected from the group consisting of SO.sub.3 H, COOH, NH.sub.2, CHO, NCS, epoxy, phthalimido, and COOZ, wherein Z represents a leaving group; M.sup.+ is a counterion selected from the group consisting of ammonium, alkali metal cations, and alkaline earth metal cations; n=1 to 4; m=1 to 4; j=2 to 10; and k=2 to 10.

摘要翻译： PCT No.PCT / EP96 / 04377 371日期：1998年5月11日 102（e）日期1998年5月11日PCT提交1996年10月8日PCT公布。 公开号WO97 / 13810PC。 日期1997年04月17日本发明涉及下式的荧光染料及其价态互变异构体：其中Q表示增加化合物的荧光量子产率的共轭部分; R 1是式 - （CH 2）j Y的官能化基团，其中Y选自SO 3 H，COOH，NH 2，CHO，NCS，环氧，邻苯二甲酰亚氨基和COOZ，其中Z表示离去基团; R2是式 - （CH2）kY'的官能化基团，其中Y'选自SO 3 H，COOH，NH 2，CHO，NCS，环氧，邻苯二甲酰亚氨基和COOZ，其中Z表示离去基团; M +是选自铵，碱金属阳离子和碱土金属阳离子的抗衡离子; n = 1〜4; m = 1〜4; j = 2〜10; k = 2〜10。

公开(公告)号：US20070231818A1

公开(公告)日：2007-10-04

申请号：US11628226

申请日：2005-06-01

申请人： Giuseppe Caputo ,  Roberto Gobetto ,  Guido Viscardi

发明人： Giuseppe Caputo ,  Roberto Gobetto ,  Guido Viscardi

IPC分类号： C12Q1/68 ,  C07D209/00 ,  G01N33/53 ,  C07F15/00

CPC分类号： C07F15/0053 ,  G01N33/533 ,  G01N2458/40 ,  Y10S436/80

摘要： The present invention relates to luminescent compounds having a functionalised linker arm, their synthesis and use in bioconjugation and labelling of biomolecules, such as for example nucleosides, nucleotides, nucleic acids (DNA, RNA or PNA) and proteins, as well as their use in the execution of in vitro and in vivo analytic and diagnostic assays.

摘要翻译： 本发明涉及具有官能化连接臂的发光化合物，它们的合成和用于生物分子的生物缀合和标记，例如核苷，核苷酸，核酸（DNA，RNA或PNA）和蛋白质，以及它们在 执行体外和体内分析和诊断测定。

公开(公告)号：US06747159B2

公开(公告)日：2004-06-08

申请号：US10038554

申请日：2002-01-02

申请人： Giuseppe Caputo ,  Leopoldo Della Ciana

发明人： Giuseppe Caputo ,  Leopoldo Della Ciana

IPC分类号： C07D40306

CPC分类号： G01N33/533 ,  C09B23/0008 ,  C09B23/0066 ,  C09B23/02 ,  C09B23/06 ,  C09B23/08

摘要： A symmetric cyanine of the formula: wherein: X is selected from the group consisting of O, S and C(CH3)2; W represents non-metal atoms required to form a benzo-condensed or a naphto-condensed ring; R1 is selected from the group consisting of (CH2)nCH3, (CH2)nSO3− and (CH2)nSO3H, wherein n is an integer selected from 0 to 6 when R1 is (CH2)nCH3, and n is an integer selected from 3 to 6 when R1 is (CH2)nSO3− or (CH2)nSO3H; R2 and R3 are independently selected from the group consisting of H, a sulphonic moiety and a sulphonate moiety and Q is a substituted polymethine bridge.

摘要翻译： 一种下式的对称花青：其中：X选自O，S和C（CH 3）2; W表示形成苯并稠合或萘并稠环所需的非金属原子;选择R1 由（CH 2）n CH 3，（CH 2）n SO 3 - 和（CH 2）n SO 3 H组成的组，其中当R 1是（CH 2）n CH 3时，n是选自0至6的整数，n是选自3至 6，当R 1是（CH 2）n SO 3 - 或（CH 2）n SO 3 H时; R 2和R 3独立地选自H，磺酸基和磺酸酯部分，Q是取代的聚甲炔桥。

公开(公告)号：US08624035B2

公开(公告)日：2014-01-07

申请号：US13125516

申请日：2009-10-21

申请人： Giuseppe Caputo ,  Ivana Miletto ,  Chiara Alessandra Bertolino ,  Gianmario Martra ,  Salvatore Coluccia

发明人： Giuseppe Caputo ,  Ivana Miletto ,  Chiara Alessandra Bertolino ,  Gianmario Martra ,  Salvatore Coluccia

IPC分类号： C07F7/02 ,  C07F5/02

CPC分类号： A61K49/0032 ,  C09B69/008 ,  G01N33/533

摘要： A silane-modified cyanine of Formula (I) includes the valence tautomers thereof: wherein R1 is a linear, saturated or unsaturated alkyl chain, having 1 to 30 carbon atoms, wherein one or more carbon atoms are optionally substituted by a 4-, 5- or 6-membered aromatic or non aromatic cyclic grouping of carbon atoms; R8 and R9 are independently selected from the group consisting of —OCH3, —OCH2CH3, —OCH2CH2CH3, —OCH(CH3)2, —OCH2CH2OCH3, —Cl, —Br, —I, Formula (II), Formula (III), —N(CH3)2, Formula (IV), Formula (V), methyl, ethyl, propyl, isopropyl. The synthesis method and the use as a fluorescent marker are for inorganic solid supports, for example silica nanoparticles, and/or for biomolecules such as peptides, antibodies, DNA, RNA, etc.

摘要翻译： 式（I）的硅烷改性花青包括其价态互变异构体：其中R1是具有1至30个碳原子的直链，饱和或不饱和的烷基链，其中一个或多个碳原子任选被4-，5 - 或6元芳族或非芳族环状基团; R 8和R 9独立地选自-OCH 3，-OCH 2 CH 3，-OCH 2 CH 2 CH 3，-OCH（CH 3）2，-OCH 2 CH 2 OCH 3，-Cl，-Br，-I，式（II），式（III） N（CH 3）2，式（IV），式（V），甲基，乙基，丙基，异丙基。 合成方法和荧光标记物的用途是用于无机固体载体，例如二氧化硅纳米颗粒，和/或用于生物分子如肽，抗体，DNA，RNA等。

公开(公告)号：US20130255997A1

公开(公告)日：2013-10-03

申请号：US13881150

申请日：2011-10-26

申请人： Adriano Zecchina ,  Fabrizio Bardelli ,  Serena Bertarione ,  Giuseppe Caputo ,  Paolo Castelli ,  Federico Cesano ,  Pierluigi Civera ,  Danilo Demarchi ,  Roberta Galli ,  Gianfranco Innocenti ,  Domenica Scarano ,  Antonino Veca ,  Marco Zanetti

发明人： Adriano Zecchina ,  Fabrizio Bardelli ,  Serena Bertarione ,  Giuseppe Caputo ,  Paolo Castelli ,  Federico Cesano ,  Pierluigi Civera ,  Danilo Demarchi ,  Roberta Galli ,  Gianfranco Innocenti ,  Domenica Scarano ,  Antonino Veca ,  Marco Zanetti

IPC分类号： H05K3/10 ,  H05K1/02

CPC分类号： H05K3/105 ,  H05K1/02 ,  H05K1/0373 ,  H05K2201/026 ,  H05K2201/0323 ,  H05K2203/107 ,  H05K2203/1136 ,  Y10T29/49155

摘要： Process for producing conductive and/or piezoresistive traces in a non-conductive polymeric substrate through laser irradiation, characterised in that said substrate is a composite polymeric material, comprising the matrix of a polymer not susceptible to carbonisation through laser irradiation and a dispersed phase comprising carbon nano fibres and/or nanotubes.

摘要翻译： 用于通过激光照射在非导电聚合物基底中产生导电和/或压阻迹线的方法，其特征在于，所述基底是复合聚合物材料，其包含通过激光照射不易于碳化的聚合物基体和包含碳的分散相 纳米纤维和/或纳米管。

公开(公告)号：US06740755B2

公开(公告)日：2004-05-25

申请号：US09995350

申请日：2001-11-27

申请人： Giuseppe Caputo ,  Leopoldo Della Ciana

发明人： Giuseppe Caputo ,  Leopoldo Della Ciana

IPC分类号： C07D23924

CPC分类号： C09B23/06 ,  C09B23/083

摘要： A process for preparing an asymmetrical indocyanine dye comprising the steps of: a) reacting a first quaternised indolenine or substituted indolenine with a compound of the formula (II) or hydrochloride thereof, wherein n is 0 or 1 Ph is phenyl or substituted phenyl X is hydrogen, halogen or alkyl, preferably chlorine, in a solvent selected from the group consisting of acetic acid, acetic anhydride and mixtures thereof in the presence of acetyl chloride, to obtain an intermediate hemicyanine, and b) further reacting said intermediate hemicyanine with a second quaternised indolenine or substituted indolenine different from said first indolenine.

摘要翻译： 一种制备不对称吲哚菁染料的方法，包括以下步骤：a）使第一季铵化的吲哚宁或取代的吲哚宁与式（II）化合物或其盐酸盐反应，其中n为0或1 Ph为苯基或取代的苯基X为 氢，卤素或烷基，优选氯，在乙酰氯存在下，在选自乙酸，乙酸酐及其混合物的溶剂中，得到中间体半花青碱，和b）进一步使所述中间体半花青与第二季铵化反应 吲哚宁或取代的吲哚宁不同于所述第一种吲哚宁。