Bacillus thuringiensis toxins and genes for controlling coleopteran pests
    4.
    发明授权
    Bacillus thuringiensis toxins and genes for controlling coleopteran pests 失效
    苏云金芽孢杆菌毒素和防治鞘翅目害虫的基因

    公开(公告)号:US06710027B2

    公开(公告)日:2004-03-23

    申请号:US09991582

    申请日:2001-11-16

    IPC分类号: A01N3718

    CPC分类号: C07K14/325

    摘要: The subject invention concerns materials and methods useful in the control of pests and, particularly, plant pests. More specifically, the subject invention concerns novel genes and pesticidal toxins referred to as 86A1(b) and 52A1(b). In preferred embodiments, the subject toxins are used for controlling flea beetles of the genus Phyllotreta. Using the genes described herein, the transformation of plants can be accomplished using techniques known to those skilled in the art. In addition, the subject invention provides toxin genes optimized for expression in plants.

    摘要翻译: 本发明涉及可用于防治害虫,特别是植物害虫的材料和方法。 更具体地,本发明涉及被称为86A1(b)和52A1(b)的新基因和杀虫毒素。 在优选的实施方案中,本发明的毒素用于控制Phyllotreta属的跳蚤甲虫。 使用本文所述的基因,可以使用本领域技术人员已知的技术来实现植物的转化。 此外,本发明提供了优化用于在植物中表达的毒素基因。

    Bacillus thuringiensis toxins and genes for controlling coleopteran pests
    5.
    发明授权
    Bacillus thuringiensis toxins and genes for controlling coleopteran pests 有权
    苏云金芽孢杆菌毒素和防治鞘翅目害虫的基因

    公开(公告)号:US06344553B1

    公开(公告)日:2002-02-05

    申请号:US09307925

    申请日:1999-05-10

    IPC分类号: C12N121

    CPC分类号: C07K14/325

    摘要: The subject invention concerns materials and methods useful in the control of pests and, particularly, the plant pests. More specifically, the subject invention concerns novel genes and pesticidal toxins referred to as 86A1(b) and 52A1(b). In preferred embodiments, the subject toxins are used for controlling flea beetles of the genus Phyllotreta. Using the genes described herein, the transformation of plants can be accomplished using techniques known to those skilled in the art. In addition, the subject invention provides toxin genes optimized for expression in plants.

    摘要翻译: 本发明涉及可用于防治害虫,特别是植物害虫的材料和方法。 更具体地,本发明涉及被称为86A1(b)和52A1(b)的新基因和杀虫毒素。 在优选的实施方案中,本发明的毒素用于控制Phyllotreta属的跳蚤甲虫。 使用本文所述的基因,可以使用本领域技术人员已知的技术来实现植物的转化。 此外,本发明提供了优化用于在植物中表达的毒素基因。

    Bacillus thuringiensis isolates, toxins, and genes selectively active
against certain coleopteran pests
    6.
    发明授权
    Bacillus thuringiensis isolates, toxins, and genes selectively active against certain coleopteran pests 失效
    苏云金芽孢杆菌分离株,毒素和对某些鞘翅目害虫选择性活性的基因

    公开(公告)号:US6071511A

    公开(公告)日:2000-06-06

    申请号:US100437

    申请日:1998-06-19

    IPC分类号: A01N63/00 C12N5/04 C12N5/10

    CPC分类号: A01N63/00

    摘要: The subject invention concerns Bacillus thuringiensis microbes with activity against select coleopteran pests, e.g., Diabrotica sp., Hypera sp., and various flea beetles. For example, the B.t. isolates of the invention are active against alfalfa weevils (AW, Hypera brunneipennis), rape flea beetles (RFB, Phyllotreta cruciferae), and corn rootworms (CRW, Diabrotica undecimpunctata undecimpunctata). Thus, these microbes can be used to control these pests. Further, genes encoding toxins active against these pests can be isolated from the B.t. isolates and used to transform other microbes. The transformed microbes then can be used to control susceptible coleopteran pests. In preferred embodiments, a gene from PS140E2 is used to transform plants so that the transformed plants are resistant to flea beetles of the genus Phyllotreta.

    摘要翻译: 本发明涉及具有针对选择性鞘翅目害虫的活性的苏云金芽孢杆菌微生物,例如Diabrotica sp。,Hypera sp。和各种跳蚤甲虫。 例如,B.t. 本发明的分离株对苜蓿象鼻虫(AW,Hypera brunneipennis),强奸跳蚤(RFB,十字花科植物)和玉米根虫(CRW,Diabrotica undecimpunctata undecimpunctata)具有活性。 因此,这些微生物可用于控制这些害虫。 此外,编码对这些害虫有活性的毒素的基因可以从B.t. 分离并用于转化其他微生物。 然后转化的微生物可用于控制易感的鞘翅目害虫。 在优选的实施方案中,使用来自PS140E2的基因来转化植物,使得转化的植物对Phyllotreta属的跳蚤甲虫具有抗性。