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    Device and method for monitoring leukocyte migration
    1.
    发明授权
    Device and method for monitoring leukocyte migration 失效
    用于监测白细胞迁移的装置和方法

    公开(公告)号：US07419823B2

    公开(公告)日：2008-09-02

    申请号：US10688904

    申请日：2003-10-21

    申请人： Gregory L. Kirk ,  Emanuele Ostuni ,  Enoch Kim ,  Olivier Schueller ,  Paul Sweetnam

    发明人： Gregory L. Kirk ,  Emanuele Ostuni ,  Enoch Kim ,  Olivier Schueller ,  Paul Sweetnam

    IPC分类号： C12M1/36 ,  C12M1/38 ,  C12M3/00 ,  C12M1/34 ,  C12M1/22

    CPC分类号： G01N33/5029 ,  G01N33/5047 ,  G01N33/5064

    摘要： A device for monitoring leukocyte migration is provided. The invention also provides a method of using the device to monitor leukocyte migration in the presence of physiological shear flow and therefore simulate physiological conditions of a blood vessel in vivo. The invention further provides a method of using the device to high-throughput screen a plurality of test agents. The present invention further provides a flexible assay system and numerous assays that can be used to test biological interactions and systems. Laminar flow gradients are employed that mimic gradient situations present in vivo.

    摘要翻译： 提供了一种用于监测白细胞迁移的装置。 本发明还提供了一种使用该装置在存在生理剪切流的情况下监测白细胞迁移并因此模拟体内血管生理条件的方法。 本发明还提供了一种使用该设备来高通量筛选多个测试代理的方法。 本发明进一步提供灵活的测定系统和可用于测试生物相互作用和系统的许多测定。 使用模拟体内存在的梯度情况的层流流动梯度。

    Method of monitoring haptotaxis
    3.
    发明授权
    Method of monitoring haptotaxis 失效
    监测触觉的方法

    公开(公告)号：US06818403B2

    公开(公告)日：2004-11-16

    申请号：US10097304

    申请日：2002-03-15

    申请人： Gregory L. Kirk ,  Matthew Brown ,  Emanuele Ostuni ,  Enoch Kim ,  Bernardo D. Aumond ,  Olivier Schueller ,  Paul Sweetnam ,  Brian Benoit

    发明人： Gregory L. Kirk ,  Matthew Brown ,  Emanuele Ostuni ,  Enoch Kim ,  Bernardo D. Aumond ,  Olivier Schueller ,  Paul Sweetnam ,  Brian Benoit

    IPC分类号： C12Q168

    CPC分类号： B82Y30/00 ,  B01J2219/00317 ,  B01J2219/00662 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00743 ,  B01L3/5085 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0829 ,  B01L2300/0887 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N33/54366 ,  G06T7/0012 ,  G06T7/70 ,  G06T2207/30072

    摘要： The present invention discloses a method of monitoring haptotaxis comprising: providing a device for monitoring haptotaxis having a housing defining a chamber. The chamber includes: a first well region including at least one first well, the first well region configured to receive a test agent therein and further including biomolecules immobilized therein; a second well region including at least one second well, the second well region configured to receive a sample comprising cells therein and further being horizontally offset with respect to the first well region in a test orientation of the device; and a channel region with biomolecules immobilized therein and including at least one channel connecting the first well region and the second well region with one another. The method also includes forming a surface concentration gradient along a longitudinal axis of the chamber by decreasing the concentration of biomolecules from the at least one first well to the at least one second well; placing a first sample comprising cells in the at least one second well; and monitoring haptotaxis of the cells.

    摘要翻译： 本发明公开了一种监测触觉性的方法，包括：提供一种用于监测触觉性的装置，其具有限定腔室的外壳。 所述腔室包括：第一井区域，其包括至少一个第一井，所述第一井区域构造成在其中接收测试剂并且还包括固定在其中的生物分子; 包括至少一个第二阱的第二阱区域，所述第二阱区域被配置为接收包含单元的样品，并且在所述器件的测试取向中进一步相对于所述第一阱区域水平偏移; 以及具有固定在其中的生物分子的通道区域，并且包括将第一阱区域和第二阱区域彼此连接的至少一个通道。 该方法还包括通过降低生物分子从至少一个第一孔到至少一个第二孔的浓度来形成沿腔室的纵向轴线的表面浓度梯度; 将包含细胞的第一样品置于所述至少一个第二孔中; 并监测细胞的触发性。

    Method of monitoring cell motility and chemotaxis
    6.
    发明授权
    Method of monitoring cell motility and chemotaxis 失效
    监测细胞运动性和趋化性的方法

    公开(公告)号：US06811968B2

    公开(公告)日：2004-11-02

    申请号：US10097322

    申请日：2002-03-15

    申请人： Gregory L. Kirk ,  Matthew Brown ,  Emanuele Ostuni ,  Enoch Kim ,  Bernardo D. Aumond ,  Olivier Schueller ,  Paul Sweetnam ,  Brian Benoit

    发明人： Gregory L. Kirk ,  Matthew Brown ,  Emanuele Ostuni ,  Enoch Kim ,  Bernardo D. Aumond ,  Olivier Schueller ,  Paul Sweetnam ,  Brian Benoit

    IPC分类号： C12Q100

    CPC分类号： B01L3/5085 ,  B01D2325/08 ,  B01J2219/00317 ,  B01J2219/00662 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00743 ,  B01L3/5025 ,  B01L3/5027 ,  B01L2200/0647 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0829 ,  B01L2300/0887 ,  B82Y30/00 ,  C12Q1/025 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N15/1468 ,  G01N33/54366 ,  G01N2015/1497 ,  G06T7/0012 ,  G06T7/70 ,  G06T2207/30072 ,  Y10S435/81

    摘要： The present invention discloses a method of monitoring chemotaxis or chemoinvasion comprising: providing a device for monitoring chemotaxis. The device includes a support member; a top member mounted to the support member by being placed in substantially fluid-tight, conformal contact with the support member. The support member and the top member are configured such that they together define a discrete assay chamber. The discrete assay chamber includes a first well region including at least one first well, the at least one first well being configured to receive a soluble test substance therein; a second well region including at least one second well, the at least one second well being configured to receive a sample comprising cells therein; and a channel region including at least one channel connecting the first well region and the second well region. Preferably, at least one second well is horizontally offset with respect to the first well region in a test orientation of the device. The method further comprises placing the soluble test substance in the at least one first well; forming a solution concentration gradient along a longitudinal axis of the chamber; placing a first sample comprising cells in the at least one second well; and monitoring chemotaxis of the cells.

    摘要翻译： 本发明公开了一种监测趋化性或化学侵蚀的方法，包括：提供用于监测趋化性的装置。 该装置包括支撑构件; 顶部构件通过与支撑构件基本上流体密封地共形接触而安装到支撑构件。 支撑构件和顶部构件被构造成使得它们在一起限定离散的测定室。 离散测定室包括包括至少一个第一阱的第一阱区，所述至少一个第一阱被配置为在其中接收可溶性测试物质; 包括至少一个第二阱的第二阱区，所述至少一个第二阱被配置为在其中接收包含电池的样品; 以及包括连接第一阱区域和第二阱区域的至少一个沟道的沟道区域。 优选地，至少一个第二井在装置的测试方向上相对于第一井区水平偏移。 该方法还包括将可溶性测试物质置于至少一个第一井中; 沿着所述室的纵向轴线形成溶液浓度梯度; 将包含细胞的第一样品置于所述至少一个第二孔中; 并监测细胞的趋化性。

    Cell motility and chemotaxis test device and methods of using same
    7.
    发明授权
    Cell motility and chemotaxis test device and methods of using same 失效
    细胞运动性和趋化性试验装置及其使用方法

    公开(公告)号：US06921660B2

    公开(公告)日：2005-07-26

    申请号：US10097351

    申请日：2002-03-15

    申请人： Gregory L. Kirk ,  Matthew Brown ,  Emanuele Ostuni ,  Enoch Kim ,  Bernardo D. Aumond ,  Olivier Schueller ,  Paul Sweetnam ,  Brian Benoit

    发明人： Gregory L. Kirk ,  Matthew Brown ,  Emanuele Ostuni ,  Enoch Kim ,  Bernardo D. Aumond ,  Olivier Schueller ,  Paul Sweetnam ,  Brian Benoit

    IPC分类号： B01L3/00 ,  C12M1/34

    CPC分类号： B82Y30/00 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502746 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2400/0406 ,  Y10S435/809

    摘要： The present invention discloses a device for monitoring chemotaxis or chemoinvasion including a housing comprising: a support member and a top member, the top member mounted to the support member by being placed in substantially fluid-tight conformal contact with the support member. The support member and the top member are configured such that they together define a discrete chamber adapted to allow a monitoring of chemotaxis or chemoinvasion therein. The discrete chamber includes a first well region including at least one first well, the at least one first well configured to received a test agent therein; a second well region including at least one second well, the at least one second well configured to receive a sample comprising cells therein; and a channel region including at least one channel connecting the first well region and the second well region with one another. The second well region is preferably horizontally offset with respect to the first well region in a test orientation of the device.

    摘要翻译： 本发明公开了一种用于监测趋化性或化学侵蚀的装置，包括：壳体，包括：支撑构件和顶部构件，顶部构件通过与支撑构件基本上不流体密封地共形接触而安装到支撑构件。 支撑构件和顶部构件被构造成使得它们一起限定适于允许监测其中的趋化性或化学侵蚀的离散室。 离散室包括包括至少一个第一井的第一井区，所述至少一个第一井构造成在其中接收测试剂; 包括至少一个第二阱的第二阱区，所述至少一个第二阱被配置为在其中接收包含电池的样品; 以及沟道区域，其包括将第一阱区域和第二阱区域彼此连接的至少一个沟道。 在设备的测试方向上，第二阱区域优选地相对于第一阱区域水平偏移。

    Method of making device for arraying biomolecules and for monitoring cell motility in real-time
    10.
    发明授权
    Method of making device for arraying biomolecules and for monitoring cell motility in real-time 失效
    制备用于排列生物分子和实时监测细胞运动性的装置的方法

    公开(公告)号：US07211209B2

    公开(公告)日：2007-05-01

    申请号：US10206329

    申请日：2002-07-29

    申请人： Enoch Kim ,  Gregory L. Kirk ,  Olivier Schueller ,  Emanuele Ostuni

    发明人： Enoch Kim ,  Gregory L. Kirk ,  Olivier Schueller ,  Emanuele Ostuni

    IPC分类号： B29C39/02

    CPC分类号： C12M23/12 ,  B01D2325/08 ,  B01J2219/00317 ,  B01J2219/00662 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00743 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/5085 ,  B01L2200/0647 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0829 ,  B01L2300/0887 ,  B82Y30/00 ,  C12M25/06 ,  C12M41/46 ,  C12Q1/025 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N15/1468 ,  G01N33/54366 ,  G01N2015/1497 ,  G06K9/0014 ,  G06T7/0012 ,  G06T7/70 ,  G06T2207/30072

    摘要： The invention relates to devices, devices for arraying biomolecules, including cells, methods for arraying biomolecules, assays for monitoring cellular movement, and systems for monitoring cellular movement. The devices include a support; a first layer configured to be placed in fluid-tight contact with the support, the first layer having an upper surface and defining a pattern of micro-orifices, each micro-orifice of the pattern of micro-orifices having walls and defining a micro-region on the support when the first layer is placed in fluid-tight contact with the support such that the walls of said each micro-orifice and the micro-region on the support together define a micro-well; and a second layer configured to be placed in fluid-tight contact with the upper surface of the first layer, the second layer defining a pattern of macro-orifices, each macro-orifice of the pattern of macro-orifices having walls and defining a macro-region when the first layer is placed in fluid-tight contact with the support and the second layer is placed in fluid-tight contact with the first layer such that the walls of the macro-orifice and the macro-region together define a macro-well.

    摘要翻译： 本发明涉及用于排列生物分子的装置，包括细胞，用于排列生物分子的方法，用于监测细胞运动的测定法和用于监测细胞运动的系统。 这些装置包括一个支架; 第一层，其被配置为与所述支撑件流体密封接触，所述第一层具有上表面并限定微孔图案，所述微孔图案的每个微孔具有壁并限定微孔， 当第一层与支撑件流体密封地接触时，支撑件上的区域在支撑件上，使得所述每个微孔的壁和支撑件上的微区域一起限定微孔; 以及第二层，其被配置为与所述第一层的上表面流体密封接触，所述第二层限定宏观孔的图案，所述宏观孔的图案的每个宏观孔口具有壁并且限定宏观 当所述第一层与所述支撑件流体密封接触时，所述第二层与所述第一层流体密封接触，使得所述宏观孔和所述宏观区域的壁一起限定宏观尺寸， 好。