利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

8 条记录 (耗时 0.033 秒)

    Methods for the identification and characterization of reticulocytes in whole blood
    2.
    发明授权
    Methods for the identification and characterization of reticulocytes in whole blood 失效
    全血中网织红细胞的鉴定和表征方法

    公开(公告)号：US5350695A

    公开(公告)日：1994-09-27

    申请号：US802593

    申请日：1991-12-05

    申请人： Gregory M. Colella ,  Daniel Ben-David ,  Albert Cupo ,  Sophie S. Fan ,  Gena Fischer ,  Grace E. Martin ,  Leonard Ornstein

    发明人： Gregory M. Colella ,  Daniel Ben-David ,  Albert Cupo ,  Sophie S. Fan ,  Gena Fischer ,  Grace E. Martin ,  Leonard Ornstein

    IPC分类号： G01N15/14 ,  G01N1/30 ,  G01N21/27 ,  G01N21/64 ,  G01N33/48 ,  G01N33/487 ,  G01N33/49 ,  G01N33/50 ,  G01N33/72 ,  G01N33/80

    CPC分类号： G01N33/80 ,  G01N1/30 ,  G01N33/5094 ,  Y10S436/80 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497

    摘要： Methods for characterizing and distinguishing reticulocytes and mature red blood cells use reagent compositions which include organic cationic dyes for staining the reticulocytes in the blood sample and buffer solutions for maintaining a pH of about 6 to about 9. The dyes may be the blue absorption dyes Oxazine 750 or New Methylene Blue. When a zwitterionic surfactant is included in the reagent composition for isovolumetric sphering of the reticulocytes and erythrocytes, and the reagent composition and whole blood sample mixture is passed through the sensing region of a flow cytometer, the light scattered and absorbed by each cell is measured, the erythrocytes can be distinguished from reticulocytes and the volume, hemoglobin concentration and the hemoglobin content of each reticulocyte or erythrocyte, and the mean cell volume, mean corpuscular hemoglobin concentration, and mean cell hemoglobin of the reticulocytes or erythrocytes are calculated from the measured cell-by-cell volume and hemoglobin concentration. One method also includes adjusting the measured absorption signals for pseudo-absorption.

    摘要翻译： 表征和区分网织红细胞和成熟红细胞的方法使用试剂组合物，其包括用于染色血液样品中的网织红细胞的有机阳离子染料和用于保持约6至约9的pH的缓冲溶液。染料可以是蓝色吸收染料恶嗪 750或新亚甲基蓝。 当用于网织红细胞和红细胞的等容体积的试剂组合物中包含两性离子表面活性剂时，并且将试剂组合物和全血样品混合物通过流式细胞仪的感测区域，测量每个细胞散射和吸收的光， 红细胞可以与网织红细胞区分开，并从测定的细胞 - 细胞计算出网织红细胞或红细胞的体积，血红蛋白浓度和每种网织红细胞或红细胞的血红蛋白含量，平均细胞体积，平均红细胞血红蛋白浓度和平均细胞血红蛋白， 细胞内体积和血红蛋白浓度。 一种方法还包括调整测量的吸收信号用于伪吸收。

    Methods for the identification and characterization of reticulocytes in whole blood
    5.
    发明授权
    Methods for the identification and characterization of reticulocytes in whole blood 失效
    全血中网织红细胞的鉴定和表征方法

    公开(公告)号：US5360739A

    公开(公告)日：1994-11-01

    申请号：US802585

    申请日：1991-12-05

    申请人： Sophie S. Fan ,  Daniel Ben-David ,  Gregory M. Colella ,  Albert Cupo ,  Gena Fischer ,  Leonard Ornstein

    发明人： Sophie S. Fan ,  Daniel Ben-David ,  Gregory M. Colella ,  Albert Cupo ,  Gena Fischer ,  Leonard Ornstein

    IPC分类号： G01N1/30 ,  G01N15/14 ,  G01N21/27 ,  G01N21/64 ,  G01N33/48 ,  G01N33/487 ,  G01N33/49 ,  G01N33/50 ,  G01N33/72 ,  G01N33/80

    CPC分类号： G01N33/80 ,  G01N1/30 ,  G01N15/1459 ,  G01N33/5094 ,  G01N1/38 ,  G01N15/1463 ,  G01N2015/0076 ,  G01N2015/1486 ,  Y10S436/80 ,  Y10T436/10 ,  Y10T436/101666

    摘要： Methods for characterizing and distinguishing reticulocytes and mature red blood cells use reagent compositions which include an organic cationic dye for staining the reticulocytes in the blood sample and a buffer solution for maintaining a pH of about 6 to about 9. The dyes may be the red excitable fluorescent dye Oxazine 750, or the blue excitable fluorescent dyes Acridine Orange or derivatives of Acridine Orange. When a zwitterionic surfactant is included in the reagent composition for isovolumetric sphering of the reticulocytes and erythrocytes, and the reagent composition and whole blood sample mixture is passed through the sensing region of a flow cytometer, the light scattered through at least one angular interval and that fluoresced by each cell is measured, the erythrocytes can be distinguished from reticulocytes and the volume, hemoglobin concentration and the hemoglobin content of each reticulocyte or erythrocyte, and the mean cell volume, mean corpuscular hemoglobin concentration, and mean cell hemoglobin of the reticulocytes and/or erythrocytes are calculated from the measured cell-by-cell volume and hemoglobin concentration.

    摘要翻译： 表征和区分网织红细胞和成熟红细胞的方法使用试剂组合物，其包括用于染色血液样品中的网织红细胞的有机阳离子染料和用于保持约6至约9的pH的缓冲溶液。染料可以是红色可激发 荧光染料Oxazine 750，或蓝色可激发荧光染料吖啶橙或吖啶橙衍生物。 当用于网织红细胞和红细胞的等容体积的试剂组合物中包含两性离子表面活性剂时，试剂组合物和全血样品混合物通过流式细胞计数器的感测区域时，散射在至少一个角度间隔的光， 测量每个细胞发荧光的红细胞，网织红细胞和网织红细胞或红细胞的体积，血红蛋白浓度和血红蛋白含量以及网织红细胞的平均细胞体积，平均红细胞血红蛋白浓度和平均细胞血红蛋白和/ 或红细胞从测量的细胞逐个体积和血红蛋白浓度计算。

    Reagent compositions and their use in the identification and characterization of reticulocytes in whole blood
    6.
    发明授权
    Reagent compositions and their use in the identification and characterization of reticulocytes in whole blood 失效
    试剂组合物及其在全血中鉴定和表征网织红细胞的用途

    公开(公告)号：US5411891A

    公开(公告)日：1995-05-02

    申请号：US961582

    申请日：1992-10-15

    申请人： Sophie S. Fan ,  Daniel Ben-David ,  Gregory M. Colella ,  Albert Cupo ,  Gena Fischer ,  Leonard Ornstein

    发明人： Sophie S. Fan ,  Daniel Ben-David ,  Gregory M. Colella ,  Albert Cupo ,  Gena Fischer ,  Leonard Ornstein

    IPC分类号： G01N1/30 ,  G01N15/14 ,  G01N21/27 ,  G01N21/64 ,  G01N33/48 ,  G01N33/487 ,  G01N33/49 ,  G01N33/50 ,  G01N33/72 ,  G01N33/80

    CPC分类号： G01N33/80 ,  G01N1/30 ,  G01N15/1459 ,  G01N33/5094 ,  G01N1/38 ,  G01N15/1463 ,  G01N2015/0076 ,  G01N2015/1486 ,  Y10S436/80 ,  Y10T436/10 ,  Y10T436/101666

    摘要： A reagent composition which include an organic cationic dye for staining the reticulocytes in the blood sample and a buffer solution for maintaining a pH of about 6 to about 9 is provided. The dyes may be the red excitable fluorescent dye Oxazine 750, or the blue excitable fluorescent dyes Acridine Orange or derivatives of Acridine Orange. A zwitterionic surfactant is included in the reagent composition for isovolumetric sphering of the reticulocytes and erythrocytes. The reagent composition and whole blood sample mixture is passed through the sensing region of a flow cytometer, the light scattered through at least one angular interval and that fluoresced by each cell is measured, the erythrocytes can be distinguished from reticulocytes and the volume, hemoglobin concentration and the hemoglobin content of each reticulocyte or erythrocyte, and the mean cell volume, mean corpuscular hemoglobin concentration, and mean cell hemoglobin of the reticulocytes and/or erythrocytes are calculated from the measured cell-by-cell volume and hemoglobin concentration.

    摘要翻译： 提供了一种试剂组合物，其包括用于染色血液样品中的网织红细胞的有机阳离子染料和用于保持约6至约9的pH的缓冲溶液。 染料可以是红色可激发荧光染料Oxazine 750，或蓝色可激发荧光染料吖啶橙或吖啶橙衍生物。 用于网织红细胞和红细胞的等容体积的试剂组合物中包含两性离子表面活性剂。 试剂组合物和全血样品混合物通过流式细胞仪的感测区域，测量散布在至少一个角度间隔内并由每个细胞发荧光的光，红细胞可与网织红细胞和体积，血红蛋白浓度 并根据测定的细胞间细胞体积和血红蛋白浓度计算网织红细胞或红细胞的血红蛋白含量，以及网织红细胞和/或红细胞的平均细胞体积，平均红细胞血红蛋白浓度和平均细胞血红蛋白。