公开(公告)号：US20090138206A1

公开(公告)日：2009-05-28

申请号：US12282440

申请日：2007-02-23

申请人： Gun Wook Park ,  Kyung-Hoon Kwon ,  Jin Young Kim ,  Jong Shin Yoo ,  Young Mok Park ,  Seung Il Kim

发明人： Gun Wook Park ,  Kyung-Hoon Kwon ,  Jin Young Kim ,  Jong Shin Yoo ,  Young Mok Park ,  Seung Il Kim

IPC分类号： G06F19/00 ,  G01N33/68

CPC分类号： G01N33/6848 ,  H01J49/004

摘要： The present invention relates to a method of analyzing protein modification. The method of invention for analyzing protein distribution and characteristics on one-dimensional gel provides the way to analyze proteins of samples on one-dimensional gel quantitatively and provides information on interactions among proteins and further can be effectively used for the development of a novel diagnostic and therapeutic method for a disease by screening a disease marker protein.

摘要翻译： 本发明涉及分析蛋白质修饰的方法。 本发明用于分析一维凝胶上的蛋白质分布和特征的方法提供了定量分析一维凝胶上样品蛋白质的方法，提供蛋白质间相互作用信息，进一步有效地用于开发新型诊断和 通过筛选疾病标记蛋白来治疗疾病的治疗方法。

公开(公告)号：US20120322160A1

公开(公告)日：2012-12-20

申请号：US13538273

申请日：2012-06-29

申请人： Kyu Hwan Park ,  Hyun Sik Kim ,  Kyung Hoon Kwon ,  Jong Shin Yoo

发明人： Kyu Hwan Park ,  Hyun Sik Kim ,  Kyung Hoon Kwon ,  Jong Shin Yoo

IPC分类号： G01N27/62

CPC分类号： G01N30/06 ,  G01N30/8686 ,  G01N30/96 ,  G01N33/6848 ,  G01N2030/8809 ,  G01N2030/8813 ,  G01N2500/00 ,  Y10T436/174614 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/25375

摘要： A method for discovering pharmacologically active substances from natural products at high speed, including: obtaining an activity profile by testing pharmacological activity of a plurality of samples; obtaining mass profiles based on mass spectra resulting from analysis of the samples by mass spectrometry; and determining molecular weight of pharmacologically active substances by comparing and analyzing the activity profile and the mass profile. The method allows fast discovery of pharmacologically active substances by performing high resolution mass spectrometry for numerous components included in an extract sample of natural products and comparing with the activity test data. The information about the intensity of the activity of the pharmacologically active substances of the natural products allows effective utilization of the natural products.

摘要翻译： 一种用于从天然产物高速发现药理活性物质的方法，包括：通过测试多个样品的药理活性来获得活性谱; 基于通过质谱分析样品得到的质谱获得质谱; 并通过比较和分析活性谱和质量曲线来确定药理学活性物质的分子量。 该方法允许通过对天然产物的提取物样品中包括的许多组分进行高分辨率质谱法与活性测试数据进行比较来快速发现药理学活性物质。 关于天然产物的药理活性物质的活性强度的信息允许有效利用天然产物。

公开(公告)号：US08518712B2

公开(公告)日：2013-08-27

申请号：US13538273

申请日：2012-06-29

申请人： Kyu Hwan Park ,  Hyun Sik Kim ,  Kyung Hoon Kwon ,  Jong Shin Yoo

发明人： Kyu Hwan Park ,  Hyun Sik Kim ,  Kyung Hoon Kwon ,  Jong Shin Yoo

IPC分类号： G01N27/62

CPC分类号： G01N30/06 ,  G01N30/8686 ,  G01N30/96 ,  G01N33/6848 ,  G01N2030/8809 ,  G01N2030/8813 ,  G01N2500/00 ,  Y10T436/174614 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/25375

摘要： A method for discovering pharmacologically active substances from natural products at high speed, including: obtaining an activity profile by testing pharmacological activity of a plurality of samples; obtaining mass profiles based on mass spectra resulting from analysis of the samples by mass spectrometry; and determining molecular weight of pharmacologically active substances by comparing and analyzing the activity profile and the mass profile. The method allows fast discovery of pharmacologically active substances by performing high resolution mass spectrometry for numerous components included in an extract sample of natural products and comparing with the activity test data. The information about the intensity of the activity of the pharmacologically active substances of the natural products allows effective utilization of the natural products.

摘要翻译： 一种用于从天然产物高速发现药理活性物质的方法，包括：通过测试多个样品的药理活性来获得活性谱; 基于通过质谱分析样品得到的质谱获得质谱; 并通过比较和分析活性谱和质量曲线来确定药理学活性物质的分子量。 该方法允许通过对天然产物的提取物样品中包括的许多组分进行高分辨率质谱法与活性测试数据进行比较来快速发现药理学活性物质。 关于天然产物的药理活性物质的活性强度的信息允许有效利用天然产物。

公开(公告)号：US08296300B2

公开(公告)日：2012-10-23

申请号：US12373495

申请日：2006-08-18

申请人： Kyung-Hoon Kwon ,  Jong Shin Yoo

发明人： Kyung-Hoon Kwon ,  Jong Shin Yoo

IPC分类号： G06F7/00

CPC分类号： G06F19/28 ,  G06F19/18 ,  G06F19/22

摘要： The present invention relates to a method for reconstructing protein database for identifying a protein and a method for screening a protein using the same, more precisely a method for reconstructing protein database, and a method for identifying a protein using the same. The method for reconstructing protein database and the method for identifying the protein of the invention are very useful for the investigation of endogenous proteins and their functions and interactions, and are further effectively used for the development of diagnostic and therapeutic agents for various diseases.

摘要翻译： 本发明涉及一种用于重建用于鉴定蛋白质的蛋白质数据库的方法和使用该蛋白质筛选蛋白质的方法，更准确地说，重建蛋白质数据库的方法，以及用于鉴定使用该蛋白质的蛋白质的方法。 重组蛋白质数据库的方法和鉴定本发明蛋白质的方法对于内源蛋白质及其功能和相互作用的研究非常有用，并且进一步有效地用于各种疾病的诊断和治疗剂的开发。

公开(公告)号：US20090319558A1

公开(公告)日：2009-12-24

申请号：US12373495

申请日：2006-08-18

申请人： Kyung-Hoon Kwon ,  Jong Shin Yoo

发明人： Kyung-Hoon Kwon ,  Jong Shin Yoo

IPC分类号： G06F17/30 ,  G06F19/00

CPC分类号： G06F19/28 ,  G06F19/18 ,  G06F19/22

摘要： The present invention relates to a method for reconstructing protein database for identifying a protein and a method for screening a protein using the same, more precisely a method for reconstructing protein database comprising the steps of i) Grouping proteins having a required homology by using the clustering software from the primary database which minimizes the overlapping of proteins and then recording sequence information of each protein group on a recording system of a computer; and ii) Designating a representative protein in each protein group and then constructing the representative protein database composed of the representative proteins, followed by recording the results containing sequence information on a recording system of a computer, and a method for identifying a protein using the same; and iii) Identifying proteins from the protein sequences of the protein group which the identified representative protein belongs to. The method for reconstructing protein database and the method for identifying the protein of the invention are very useful for the investigation of endogenous proteins and their functions and interactions, and are further effectively used for the development of diagnostic and therapeutic agents for various diseases.

摘要翻译： 本发明涉及一种用于重建用于鉴定蛋白质的蛋白质数据库的方法和使用该蛋白质筛选蛋白质的方法，更准确地说，一种重建蛋白质数据库的方法，包括以下步骤：i）通过使用聚类分组具有所需同源性的蛋白质 来自主数据库的软件，其最小化蛋白质的重叠，然后在计算机的记录系统上记录每个蛋白质组的序列信息; 和ii）指定每个蛋白质组中的代表性蛋白质，然后构建由代表性蛋白质组成的代表性蛋白质数据库，然后将含有序列信息的结果记录在计算机的记录系统上，以及用于鉴定使用其的蛋白质的方法 ; 和iii）从鉴定的代表性蛋白质所属的蛋白质组的蛋白质序列鉴定蛋白质。 重组蛋白质数据库的方法和鉴定本发明蛋白质的方法对于内源蛋白质及其功能和相互作用的研究非常有用，并且进一步有效地用于各种疾病的诊断和治疗剂的开发。

公开(公告)号：US20120107858A1

公开(公告)日：2012-05-03

申请号：US13266893

申请日：2009-11-19

申请人： Jong Shin Yoo ,  Yeong Hee Ahn ,  Ju Yeon Lee ,  Jin Young Kim

发明人： Jong Shin Yoo ,  Yeong Hee Ahn ,  Ju Yeon Lee ,  Jin Young Kim

IPC分类号： C12Q1/37 ,  C07K16/18

CPC分类号： C07K14/47 ,  C07K14/4748 ,  G01N33/574 ,  G01N2333/4728

摘要： The present invention relates to a cancer diagnosis method using peptides containing information on the glycosylation of a glycoprotein involving cancer development. More particularly, the present invention relates to a cancer diagnosis method which obtains peptides from the glycoprotein involving cancer development through a hydrolysis process using an enzyme, and quantitatively detects, from among the thus-obtained peptides, glycosylation-related specific peptides which are influenced by the glycosylation of proteins and show specific quantitative changes in the hydrolysis process, to thereby select glycosylation-related specific peptides which show specific quantitative changes in accordance with cancer development. The cancer diagnosis method of the present invention uses the thus-selected glycosylation-related specific peptides as a marker.

摘要翻译： 本发明涉及使用含有涉及癌症发生的糖蛋白的糖基化信息的肽的癌症诊断方法。 更具体地，本发明涉及通过使用酶的水解方法从涉及癌症发展的糖蛋白中获得肽的癌症诊断方法，并且从如此获得的肽定量检测受到影响的糖基化相关特异性肽 蛋白质的糖基化，并在水解过程中显示出特定的定量变化，从而选择根据癌症发展显示特定定量变化的糖基化相关特异性肽。 本发明的癌症诊断方法使用如此选择的糖基化相关特异性肽作为标记物。

公开(公告)号：US07250266B2

公开(公告)日：2007-07-31

申请号：US10907945

申请日：2005-04-21

申请人： Yeong Hee Ahn ,  Jong Shin Yoo ,  Jin Young Kim ,  Kun Cho

发明人： Yeong Hee Ahn ,  Jong Shin Yoo ,  Jin Young Kim ,  Kun Cho

IPC分类号： G01N33/00

CPC分类号： G01N33/6848 ,  A61K31/155 ,  G01N33/6803 ,  G01N33/6842 ,  G01N2458/15

摘要： Disclosed is a method of analyzing mass of the phosphoproteins or phosphopeptides and of analyzing phosphorylated positions at a phosphoprotein or phosphopeptide, comprising the steps of: 1) dephosphorylating at least one Ser and/or Thr residue of the phosphoprotein or phosphopeptide; 2) tagging the dephosphorylated amino acid residues with a tag having a R-L-G moiety wherein R is a nucleophilic functional group that selectively bind with dephosphorylated amino acid residues, G is selected from the group consisting of guanidine moiety or protected guanidine moiety such as a mono-N-protected guanidino group, a di-N,N′-protected guanidino group and an N′-protected guanidino group, and L is a linker linking the R and the G; and 3) subjecting the tagged proteins or peptides to mass spectrometry. The method is capable of precisely analyzing mass of phosphoproteins of trace amounts as well as positions of phosphoryated amino acids.

摘要翻译： 公开了一种分析磷蛋白或磷酸肽的质量并分析磷蛋白或磷酸肽的磷酸化位置的方法，包括以下步骤：1）使磷酸蛋白或磷酸肽的至少一个Ser和/或Thr残基去磷酸化; 2）用具有RLG部分的标签标记去磷酸化的氨基酸残基，其中R是与去磷酸化的氨基酸残基选择性结合的亲核官能团，G选自胍部分或被保护的胍部分，例如单 - N-保护的胍基，二-N，N'保护的胍基和N'保护的胍基，L是连接R和G的连接基团; 和3）对标记的蛋白质或肽进行质谱分析。 该方法能够精确分析痕量的磷蛋白质量以及磷酸化氨基酸的位置。

公开(公告)号：US07629744B2

公开(公告)日：2009-12-08

申请号：US11574660

申请日：2004-10-08

申请人： Yeong Hee Ahn ,  Jong Shin Yoo ,  Jae Yong Lee ,  Jin Young Kim ,  Kun Cho

发明人： Yeong Hee Ahn ,  Jong Shin Yoo ,  Jae Yong Lee ,  Jin Young Kim ,  Kun Cho

IPC分类号： H01J17/26

CPC分类号： G01N33/6842 ,  G01N33/6848

摘要： The present invention relates to a method for phosphorylation site-specific labeling of phosphoproteome with a site-specific tagging reagent and analyzing of the resulting labeled one, more especially, a method for in-situ tagging of phosphorylation sites of phosphoproteins retained in polymeric gel with a nucleophilic tagging reagent. It also relates a method for generating new proteolytic cleavable sites at formerly phosphorylation sites by a proper choice of a nucleophilic tagging reagent. It also relates to a method for phosphopeptides analysis and phosphorylation site identification by in-gel digestion of the previously in-gel tagged proteins and subsequent mass analysis of the resulting peptides. The invention provides in-gel chemical tagging method for phosphoaminoacid residue of phosphoproteins retained in polymeric gel matrix. Phosphoprotein can be immobilized into gel matrix by a variety of methods such as gel electrophoresis. The immobilized phosphoproteins are retained in gel matrix during tagging reaction to phosphorylated aminoacid residue of phosphoproteins, and the resulting tagged proteins are also retained in gel matrix till following purification steps like washing of the tagging reagents are accomplished. The tagged proteins is digested by protease, and the resulting digested peptides is released from gel into solution and applied for peptide mass analysis.

摘要翻译： 本发明涉及用位点特异性标记试剂对磷酸化蛋白质进行磷酸化位点特异性标记的方法，并分析得到的标记物，更具体地说，一种用于原位标记保留在聚合物凝胶中的磷酸化蛋白的磷酸化位点的方法， 亲核标记试剂。 它还涉及通过适当选择亲核标记试剂在以前的磷酸化位点产生新的蛋白水解可切割位点的方法。 它还涉及通过凝胶内消化先前凝胶内标记蛋白的磷酸肽分析和磷酸化位点鉴定的方法以及随后对所得肽的质量分析。 本发明提供了凝胶化学标记方法，用于保留在聚合物凝胶基质中的磷酸蛋白的磷酸氨基酸残基。 磷酸蛋白可以通过各种方法如凝胶电泳固定在凝胶基质中。 在磷酸化蛋白质的磷酸化氨基酸残基的标签反应期间，将固定的磷酸蛋白质保留在凝胶基质中，并且所得到的标记蛋白质也保留在凝胶基质中，直到完成标记试剂的洗涤之后的纯化步骤。 标记的蛋白质被蛋白酶消化，并将得到的消化肽从凝胶释放到溶液中并用于肽质量分析。

公开(公告)号：US08796618B2

公开(公告)日：2014-08-05

申请号：US13511157

申请日：2010-11-16

申请人： Seung Yong Kim ,  Hyun Sik Kim ,  Jong Shin Yoo

发明人： Seung Yong Kim ,  Hyun Sik Kim ,  Jong Shin Yoo

IPC分类号： H01J49/38 ,  H01J49/02

CPC分类号： H01J49/38 ,  H01J49/022

摘要： The present invention relates to an apparatus and method for controlling a pipeline-type ion cyclotron resonance mass spectrometer, in which an ion trap unit of the ion cyclotron resonance mass spectrometer is capable of using two digitizers at the same time, thus enabling a measurement process for detecting an electrical signal which indicates the mass of ions corresponding to a specific purpose, and another measurement process for detecting another electrical signal which indicates the mass of ions corresponding to another specific purpose, to be simultaneously performed. Accordingly, it is an aim of the present invention to provide an apparatus and method for controlling a pipeline-type ion cyclotron resonance mass spectrometer, which can overcome the problems of time delay among control procedures, and can present a signal detection step wherein an excitation electrode is utilized to improve the sensitivity and speed of signal detection.

摘要翻译： 本发明涉及一种用于控制管道型离子回旋共振质谱仪的装置和方法，其中离子回旋共振质谱仪的离子阱单元能够同时使用两个数字化仪，因此能够进行测量过程 用于检测指示对应于特定目的的离子质量的电信号，以及用于检测指示与另一特定目的相对应的离子质量的另一电信号的另一测量处理。 因此，本发明的目的是提供一种用于控制管道型离子回旋共振质谱仪的装置和方法，其可以克服控制过程之间的时间延迟的问题，并且可以呈现信号检测步骤，其中激发 电极用于提高信号检测的灵敏度和速度。

公开(公告)号：US08723112B2

公开(公告)日：2014-05-13

申请号：US13699577

申请日：2011-10-31

申请人： Seung Yong Kim ,  Hyun Sik Kim ,  Myoung Choul Choi ,  Jong Shin Yoo

发明人： Seung Yong Kim ,  Hyun Sik Kim ,  Myoung Choul Choi ,  Jong Shin Yoo

IPC分类号： H01J49/02

CPC分类号： H01J49/02 ,  H01J49/022 ,  H01J49/38

摘要： Provided are a controller and a control method for improving signal performance of an ion cyclotron resonance mass spectrometer. The controller and control method apply electric signals for causing ions injected into an ion trap of the ion cyclotron resonance mass spectrometer to be injected to the center of the trap as close as possible to trap electrodes, and adjust biased ion motion by appropriately adjusting signals of trap electrodes for causing the injected ions to make ion motion, thereby improving the fidelity of ion signals. The control method for improving signal performance of an ion cyclotron resonance mass spectrometer includes an ion position adjustment process and an ion signal detection process.

摘要翻译： 提供了一种用于改善离子回旋共振质谱仪的信号性能的控制器和控制方法。 控制器和控制方法应用电信号，使离子回旋共振质谱仪的离子阱中注入的离子尽可能的靠近陷阱的中心捕获电极，并通过适当调整信号 用于引起注入的离子离子运动的捕获电极，从而提高离子信号的保真度。 用于提高离子回旋共振质谱仪的信号性能的控制方法包括离子位置调整处理和离子信号检测处理。