公开(公告)号：US20180045571A1

公开(公告)日：2018-02-15

申请号：US15556190

申请日：2015-03-11

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Manabu SHIOZAWA ,  Koichi WATANABE ,  Masataka SHIRAI

IPC: G01J3/44 ,  G01N21/65 ,  G01J3/02

CPC classification number: G01J3/4412 ,  G01J3/0208 ,  G01J3/0218 ,  G01J3/0237 ,  G01N21/65 ,  G01N2021/653

Abstract: In multiplex CARS, a fingerprint region is a region where signals are densely concentrated. Information about the intensities and spatial distribution of these signals is important in cell analysis. However, there has been a problem in that the signal intensities are low. Accordingly, mechanisms 702 and 703 for adjusting the power branching ratio between light that enters a photonic crystal fiber 705 and pumping light; mechanisms 710 and 711 for adjusting the divergence/convergence state of the pumping light; and mechanisms 706 and 2101 for adjusting the divergence/convergence state of Stokes light are provided to enable adjustment for emphasizing a desired wavelength band.

公开(公告)号：US20180265918A1

公开(公告)日：2018-09-20

申请号：US15775408

申请日：2015-12-01

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Masataka SHIRAI ,  Tomoyuki SAKAI ,  Kenko UCHIDA

IPC: C12Q1/6837 ,  C12Q1/6809 ,  C12N15/10 ,  C40B40/08 ,  C40B50/06 ,  G01N33/543

CPC classification number: C12Q1/6837 ,  C12N15/1003 ,  C12N15/1093 ,  C12N15/1096 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6809 ,  C40B40/08 ,  C40B50/06 ,  G01N33/54306

Abstract: The purpose of the present invention is to provide a single cell analysis device in which the improvement of the nucleic acid capturing efficiency and the improvement of the cell capturing efficiency are both achieved and a highly accurate single cell analysis data is thereby obtained. The present invention relates to an improvement of a cell analysis device including a two-dimensional array chip having a plurality of cell capture parts capable of capturing a single cell in each of the capture parts, and nucleic acid capture parts corresponding to the respective cell capture parts, the nucleic acid capture parts being capable of capturing a nucleic acid extracted from the cell captured by the cell capture part.

公开(公告)号：US20160299080A1

公开(公告)日：2016-10-13

申请号：US15036241

申请日：2014-09-22

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Manabu SHIOZAWA ,  Koichi WATANABE ,  Masataka SHIRAI ,  Kentaro OSAWA

IPC: G01N21/65

CPC classification number: G01N21/65 ,  G01N2021/653 ,  G01N2201/06113 ,  G01N2201/0638

Abstract: To measure a surface state, reflective CARS is suitable in terms of the signal intensity. However, with the reflective CARS, it has been difficult to identify the surface position because the shape information is not acquired. Thus, a reflective CARS microscope is combined with a high-resolution phase sensor. The surface position is identified with the phase sensor, and reflected CARS generated from the surface is detected, so that composition analysis is performed.

Abstract translation: 为了测量表面状态，反射CARS在信号强度方面是合适的。 然而，对于反射CARS，由于未获取形状信息，因此难以识别表面位置。 因此，反射CARS显微镜与高分辨率相位传感器组合。 用相位传感器识别表面位置，并检测从表面产生的反射CARS，从而进行组成分析。