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    NUCLEIC ACID ANALYSIS DEVICE
    1.
    发明申请
    NUCLEIC ACID ANALYSIS DEVICE 有权
    核酸分析装置

    公开(公告)号：US20150202618A1

    公开(公告)日：2015-07-23

    申请号：US14421239

    申请日：2013-07-08

    Applicant: Hitachi High-Technologies Corporation

    Inventor： Takanobu Haga ,  Yoshitaka Kodama ,  Tomohiro Shoji ,  Takamichi Muramatsu ,  Shuhei Yamamoto

    IPC: B01L3/00 ,  B01L99/00

    CPC classification number: B01L3/50 ,  B01L3/5027 ,  B01L99/00 ,  B01L2300/0636 ,  C12Q1/6869 ,  C12Q2535/122 ,  G01N21/6428 ,  C12Q2563/107 ,  C12Q2565/629

    Abstract: A nucleic acid analysis device which can determine a DNA sequence has a flowcell in which two or more DNA fragment clusters of two or more DNA fragments having identical nucleotide sequences are immobilized. At least a part of the flowcell is made of a transparent material. An irradiation unit irradiates a part in which the DNA fragment clusters are immobilized. The device has a lens for collecting fluorescence, and a light-detection element. A solution containing only dATP having a fluorescently modified phosphate terminal among four bases, a solution containing only dCTP having a fluorescently modified phosphate terminal among the four bases, a solution containing only dGTP having a fluorescently modified phosphate terminal among the four bases, a solution containing only dTTP having a fluorescently modified phosphate terminal among the four bases, and a buffer solution are sent sequentially to where the DNA fragment clusters are immobilized.

    Abstract translation: 可以确定DNA序列的核酸分析装置具有其中固定有两个或更多个具有相同核苷酸序列的DNA片段的两个或更多个DNA片段的流式细胞池。 流通池的至少一部分由透明材料制成。 照射单元照射固定有DNA片段簇的部分。 该装置具有用于收集荧光的透镜和光检测元件。 在四个碱中仅含有具有荧光改性磷酸酯末端的dATP的溶液，仅含有四碱基中具有荧光改性磷酸酯末端的dCTP的溶液，仅含有四碱基中具有荧光改性磷酸酯末端的dGTP的溶液，含有 在四个碱基中只有具有荧光改性的磷酸酯末端的dTTP和缓冲溶液依次送到固定有DNA片段簇的位置。

    FLOW CELL FOR BIOMATERIAL ANALYSIS AND BIOMATERIAL ANALYSIS DEVICE
    2.
    发明申请
    FLOW CELL FOR BIOMATERIAL ANALYSIS AND BIOMATERIAL ANALYSIS DEVICE 审中-公开
    用于生物体分析和生物物质分析装置的流动细胞

    公开(公告)号：US20150176070A1

    公开(公告)日：2015-06-25

    申请号：US14414235

    申请日：2013-07-11

    Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

    Inventor： Yuichiro Ota ,  Tomohiro Shoji ,  Michiru Fujioka ,  Takanobu Haga

    IPC: C12Q1/68 ,  G01N21/03 ,  G01N15/14 ,  G01N21/64

    CPC classification number: C12Q1/6869 ,  G01N15/1436 ,  G01N15/1459 ,  G01N15/1484 ,  G01N21/0332 ,  G01N21/05 ,  G01N21/6428 ,  G01N21/645 ,  G01N33/5436 ,  G01N2015/0038 ,  G01N2015/1006 ,  G01N2021/6482 ,  G01N2201/0231 ,  G01N2201/061

    Abstract: In a biomaterial analysis, erroneous detection of a particle emitting fluorescence is prevented, and highly sensitive and highly accurate optical detection in biomaterial analysis is performed. A flow cell (104) for biomaterial analysis includes: a light-transmissive upper substrate (310); an antireflective lower substrate (313); and an inner layer section interposed between the upper substrate (310) and the lower substrate (313) and including a flow path (311) in which a particle (312) configured to emit fluorescence is provided. A biomaterial analysis device includes: a flow cell (104) for biomaterial analysis as described above; and an irradiation unit configured to irradiate excitation light; and an optical detection unit (106) configured to detect fluorescence emitted by the particle (312).

    Abstract translation: 在生物材料分析中，防止发射荧光的颗粒的错误检测，并且进行生物材料分析中的高灵敏度和高精度的光学检测。 用于生物材料分析的流动池（104）包括：透光上基板（310）; 抗反射下基板（313）; 以及插入在上基板（310）和下基板（313）之间的内层部分，并且包括设置有发射荧光的颗粒（312）的流路（311）。 生物材料分析装置包括：如上所述的用于生物材料分析的流动池（104）; 以及被配置为照射激发光的照射单元; 以及被配置为检测由所述粒子（312）发射的荧光的光学检测单元（106）。

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